FastQCFastQC Report
Fri 27 May 2016
SRR2139989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8074558
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97250.045.1062241
GTATCAA165000.035.1007541
CTTATAC68000.032.0390321
ATCAACG197450.028.4639663
TCAACGC198550.028.3055694
CAACGCA201650.027.9326325
TATCAAC208350.027.4363022
AACGCAG208850.027.0898026
TATACAC90500.026.4302163
GTACATG269050.024.1062341
TACATGG270300.023.7781662
ACGCAGA241000.023.493167
TTATACA96800.023.1547472
CGCAGAG246100.023.0063068
ACATGGG279050.022.5381933
CATGGGG201000.020.2006654
GCAGAGT297000.019.0071569
TACACAT123050.018.4527345
GAGTACT146800.018.24442712-13
ACACATC118000.018.1793296
Produced by FastQC (version 0.11.5)