FastQCFastQC Report
Fri 27 May 2016
SRR2139988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11745341
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140650.047.661891
GTATCAA243900.035.1547051
TCAACGC292550.028.410854
ATCAACG292650.028.3582423
CAACGCA297150.027.9424365
TATCAAC311950.027.1331862
AACGCAG309900.026.8748686
ACGCAGA360700.023.0897887
CGCAGAG366450.022.7390338
GTACATG402450.021.5784991
TACATGG395650.021.2647862
ACATGGG401700.020.3437353
GTGGTAT70800.019.9649851
TGGTATC67350.019.2282452
GCAGAGT441900.018.7895349
GAGTACT227950.017.80383312-13
AGAGTAC394600.017.6259210-11
CATGGGG266950.017.5651844
CAGAGTA431200.017.19469610-11
AGTACTT255550.015.39246112-13
Produced by FastQC (version 0.11.5)