##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11745341
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4468504575559	33.0	31.0	34.0	31.0	34.0
2	32.642566273725045	34.0	31.0	34.0	31.0	34.0
3	32.73088427147411	34.0	31.0	34.0	31.0	34.0
4	36.16579850682922	37.0	37.0	37.0	35.0	37.0
5	36.009267674731625	37.0	35.0	37.0	35.0	37.0
6	36.03451649466797	37.0	35.0	37.0	35.0	37.0
7	36.05232023489143	37.0	35.0	37.0	35.0	37.0
8	36.038736380663615	37.0	35.0	37.0	35.0	37.0
9	37.8859586963035	39.0	38.0	39.0	35.0	39.0
10-11	37.76551247000832	39.0	37.5	39.0	35.0	39.0
12-13	37.79280444050113	39.0	38.0	39.0	35.0	39.0
14-15	39.09735328246323	40.0	38.5	41.0	36.0	41.0
16-17	39.066511478891925	40.0	38.0	41.0	36.0	41.0
18-19	39.10053492699787	40.0	38.5	41.0	36.0	41.0
20-21	39.14085607096777	40.0	39.0	41.0	36.0	41.0
22-23	39.10305459810073	40.0	39.0	41.0	36.0	41.0
24-25	39.09120701643543	40.0	39.0	41.0	36.0	41.0
26-27	38.99119383575173	40.0	39.0	41.0	35.5	41.0
28-29	38.91473646678178	40.0	38.0	41.0	35.0	41.0
30-31	38.79662951870484	40.0	38.0	41.0	35.0	41.0
32-33	38.733930321553075	40.0	38.0	41.0	35.0	41.0
34-35	38.67611633869086	40.0	38.0	41.0	35.0	41.0
36-37	38.61603892007256	40.0	38.0	41.0	35.0	41.0
38-39	38.542023920216636	40.0	38.0	41.0	35.0	41.0
40-41	38.521864476270196	40.0	38.0	41.0	34.5	41.0
42-43	38.46851997040391	40.0	38.0	41.0	34.0	41.0
44-45	38.44114378872441	40.0	38.0	41.0	34.0	41.0
46-47	38.3399699240122	40.0	38.0	41.0	34.0	41.0
48-49	38.33105818493141	40.0	38.0	41.0	34.0	41.0
50-51	38.27230611355802	40.0	38.0	41.0	34.0	41.0
52-53	38.10972732125221	40.0	37.0	41.0	33.5	41.0
54-55	37.97400257810399	40.0	37.0	41.0	33.0	41.0
56-57	37.799480547467965	39.5	37.0	41.0	33.0	41.0
58-59	37.58804987714669	39.0	36.0	41.0	33.0	41.0
60-61	37.43988774245394	39.0	36.0	41.0	33.0	41.0
62-63	37.27952650507717	39.0	35.5	40.0	32.5	41.0
64-65	37.01670976242664	38.5	35.0	40.0	32.0	41.0
66-67	36.816070959220966	38.0	35.0	40.0	32.0	41.0
68-69	36.53962847678626	37.5	35.0	40.0	31.5	41.0
70-71	36.21382325895742	37.0	35.0	39.5	31.0	41.0
72-73	35.95332490650269	36.5	35.0	39.0	31.0	41.0
74-75	35.59729971779008	36.0	34.0	39.0	31.0	40.0
76-77	33.56382313523034	34.5	32.0	36.5	28.0	39.0
78-79	34.7325442350746	35.0	34.0	37.0	30.5	39.0
80-81	34.70902137149885	35.0	34.0	37.0	31.0	39.0
82-83	34.460275896748996	35.0	34.0	36.5	31.0	39.0
84-85	34.18210290735736	35.0	34.0	36.0	30.0	37.0
86-87	33.950112800387075	35.0	34.0	36.0	30.0	37.0
88-89	33.738059359442005	35.0	34.0	35.0	30.0	36.5
90-91	33.675014013981695	35.0	34.0	35.0	30.0	36.0
92-93	33.587876404796525	35.0	34.0	35.0	30.0	36.0
94-95	33.501436974975505	35.0	33.5	35.0	30.0	36.0
96-97	33.50459632028646	35.0	33.0	35.0	30.0	35.5
98-99	33.56856392457379	35.0	33.0	35.0	30.0	35.0
100-101	32.293937278174184	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	16.0
20	53.0
21	261.0
22	956.0
23	3131.0
24	8044.0
25	17539.0
26	32894.0
27	53577.0
28	79967.0
29	112526.0
30	152578.0
31	200946.0
32	267456.0
33	369519.0
34	548466.0
35	859417.0
36	1556556.0
37	2866225.0
38	3287019.0
39	1302847.0
40	25342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.90658236316851	20.37017912038484	13.955056732707888	23.76818178373876
2	14.646567461386798	21.181533708279876	41.91231080154763	22.25958802878569
3	18.610585950986348	26.147051173277013	30.07543148863419	25.166931387102448
4	12.493185562238049	17.887759707450215	39.0165737172957	30.602481013016035
5	13.062900951603016	38.17262319972203	35.600045260507166	13.164430588167782
6	29.92547570316868	38.04439342828027	18.886895750699196	13.143235117851852
7	26.700076225969088	32.5883088451838	23.13032886827211	17.581286060574996
8	24.25154559196168	37.36678331436694	21.14939561551996	17.232275478151415
9	25.70617692919525	16.823098220000773	21.276127290557252	36.19459756024672
10-11	24.19468954518256	26.986882564951177	28.91186794631786	19.90655994354841
12-13	24.921028173925837	24.49474378237883	29.170577441515473	21.413650602179864
14-15	21.95785681093213	25.900203978804186	26.78833933940428	25.3535998708594
16-17	21.564414583218838	29.06457560514979	27.4731765985093	21.897833213122073
18-19	21.56156215473012	28.34273606871014	29.10131770546296	20.994384071096786
20-21	22.42144528566988	27.472466141269962	28.975467060051496	21.130621513008666
22-23	22.128815762858377	27.40885015026395	28.81794272560636	21.64439136127131
24-25	21.83049471445521	27.49764258139966	28.940740907612227	21.731121796532896
26-27	21.804385362845142	27.68050216197409	28.813938333052942	21.70117414212782
28-29	21.65522292332454	27.849402568602244	28.861620193622468	21.633754314450748
30-31	21.95528091352426	27.6728582781466	28.790291181652407	21.58156962667674
32-33	21.770010382467085	27.81163575717556	28.686691512016516	21.73166234834084
34-35	21.865094418696813	27.876778125407853	28.71847075345904	21.53965670243629
36-37	21.861863601543664	27.79281523002819	28.6806441696266	21.664676998801543
38-39	21.748091741465334	27.90153201171307	28.760210396656987	21.590165850164606
40-41	21.92753072994658	27.74941799914566	28.655584252485365	21.667467018422393
42-43	21.863678830668245	27.827787767547452	28.47631695292357	21.83221644886073
44-45	21.90153483078982	27.99669374456525	28.300366794652515	21.801404629992412
46-47	21.982619905015866	27.940479839996478	28.06844944076418	22.008450814223472
48-49	21.970196468233418	28.013773545034965	28.04423278714989	21.971797199581726
50-51	21.9496899202694	28.1287073891908	27.940164664339783	21.981438026200017
52-53	22.066114503625656	28.08838039617626	27.822650277265726	22.022854822932363
54-55	22.065593378108847	28.006263724329006	27.85042573095817	22.077717166603975
56-57	22.10774393343248	28.069419605037744	27.797859522957886	22.024976938571886
58-59	22.03938829352767	28.09255451198563	27.86753573704548	22.00052145744122
60-61	22.12346082727604	28.09216937470892	27.81651700832932	21.96785278968572
62-63	22.116282656494555	28.09178615876998	27.754728019673426	22.037203165062042
64-65	22.055746436763894	28.0673016758345	27.752646958946087	22.12430492845552
66-67	22.14001459842883	28.081249337143987	27.66109641508655	22.117639649340628
68-69	22.080710326976703	28.09403271269006	27.68709666114279	22.138160299190442
70-71	22.121653832388787	27.877751116682703	27.754314764925258	22.246280286003252
72-73	22.11569812626514	27.940303210309654	27.769902216360197	22.17409644706501
74-75	22.052149078238532	28.062560707272837	27.6720249338884	22.213265280600233
76-77	22.09299688835898	28.020108227940945	27.631358650759523	22.255536232940557
78-79	22.094485372528798	28.00042507335217	27.606119742485223	22.29896981163381
80-81	22.06747483527785	28.06099278212999	27.6376835627094	22.23384881988276
82-83	22.152801399537687	27.994039062462665	27.71512852626718	22.13803101173247
84-85	22.24751357248821	27.991379479509256	27.60007781070704	22.1610291372955
86-87	22.062701691691935	28.02620321028227	27.693178525829282	22.217916572196515
88-89	22.130419692115446	27.982600256744334	27.651048758021147	22.235931293119076
90-91	22.168323103710737	27.98353697637384	27.685050017970187	22.16308990194524
92-93	22.164312297104104	28.008323791155398	27.641791498950237	22.18557241279026
94-95	22.026894115890393	28.05533333471043	27.70565494098008	22.2121176084191
96-97	22.045391690416903	28.07364073786569	27.730876917443037	22.150090654274372
98-99	22.249980451167016	28.668263321777797	27.78574002859142	21.296016198463764
100-101	22.985473569305913	31.286675387114794	24.846910711459394	20.8809403321199
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3239.0
1	2731.5
2	3224.5
3	4497.5
4	6165.5
5	9979.0
6	13099.5
7	15282.5
8	16694.5
9	16206.5
10	14579.0
11	13113.5
12	14268.5
13	19110.5
14	30186.0
15	44487.0
16	55221.0
17	60796.5
18	61595.5
19	58743.0
20	54464.5
21	51060.0
22	51196.0
23	57483.0
24	69534.5
25	86456.0
26	108636.5
27	133906.0
28	161025.5
29	188606.5
30	214118.0
31	240062.0
32	266699.0
33	291035.5
34	315545.0
35	341580.0
36	367675.0
37	389873.5
38	407204.5
39	422956.5
40	438629.0
41	453116.5
42	463243.0
43	472910.5
44	483070.0
45	492739.0
46	499537.5
47	503210.0
48	503481.0
49	496555.5
50	485919.5
51	468979.5
52	445613.5
53	420723.5
54	393200.0
55	361232.0
56	328865.5
57	295045.5
58	259180.5
59	223800.0
60	188490.5
61	154638.5
62	123170.5
63	95836.0
64	71529.5
65	52006.5
66	38196.5
67	28403.5
68	21053.5
69	14883.5
70	10423.5
71	7435.0
72	5270.5
73	3663.5
74	2530.0
75	1712.0
76	1178.5
77	819.0
78	559.5
79	380.5
80	274.0
81	194.5
82	142.0
83	96.5
84	61.5
85	41.0
86	31.5
87	25.5
88	20.0
89	15.5
90	9.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017028028390150612
3	0.0020944474919885256
4	4.938128233143678E-4
5	1.7028028390150616E-5
6	3.746166245833135E-4
7	0.0
8	2.554204258522592E-5
9	4.6827078072914183E-4
10-11	4.8529880911929246E-4
12-13	1.1068218453597898E-4
14-15	2.554204258522592E-5
16-17	3.9590166007100175E-4
18-19	0.0
20-21	5.10859641398669E-5
22-23	4.2575530054705296E-6
24-25	0.0
26-27	6.816272777693781E-5
28-29	2.557810241600098E-4
30-31	5.933448393853938E-4
32-33	3.123630603552612E-4
34-35	8.454751286967649E-4
36-37	9.913846518569605E-5
38-39	9.976905199576928E-5
40-41	3.2768365926352094E-4
42-43	0.0012546056131847517
44-45	3.819883943930678E-4
46-47	1.3016427314766252E-4
48-49	3.763745095057917E-4
50-51	0.0
52-53	4.4811728143968904E-4
54-55	6.75869838655886E-4
56-57	6.447745470494105E-4
58-59	6.228163689644042E-4
60-61	6.820653977752563E-4
62-63	1.0188144958090824E-4
64-65	6.663255014773073E-4
66-67	0.002450836103974667
68-69	0.0012115664342428325
70-71	2.2789132561548128E-4
72-73	0.0
74-75	4.1427348212138053E-5
76-77	1.0473907114563753E-5
78-79	8.687864420154447E-4
80-81	2.037972577684565E-4
82-83	1.2489650559669387E-4
84-85	8.739611679814927E-4
86-87	2.6156616357846997E-4
88-89	5.639014219112695E-6
90-91	3.431606537484982E-5
92-93	9.291492349559415E-5
94-95	5.606720983005734E-4
96-97	6.016144202644914E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	266.0
20-21	861.0
22-23	2016.0
24-25	4250.0
26-27	6552.0
28-29	11954.0
30-31	26963.0
32-33	32418.0
34-35	44471.0
36-37	68342.0
38-39	82798.0
40-41	83603.0
42-43	96276.0
44-45	115176.0
46-47	115816.0
48-49	108093.0
50-51	99080.0
52-53	94378.0
54-55	101864.0
56-57	106698.0
58-59	107215.0
60-61	105161.0
62-63	100201.0
64-65	104867.0
66-67	110641.0
68-69	113878.0
70-71	112252.0
72-73	107111.0
74-75	108446.0
76-77	106941.0
78-79	114533.0
80-81	116501.0
82-83	111552.0
84-85	111318.0
86-87	115644.0
88-89	122921.0
90-91	131100.0
92-93	135419.0
94-95	150962.0
96-97	232302.0
98-99	981205.0
100-101	7143296.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.57101735660216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85505313145397	76.88988311090057
2	7.150411177565903	12.237359179646678
3	1.709139902179253	4.387585207027264
4	0.6188508561335664	2.11822789405414
5	0.2753529792894466	1.1781117284984672
6	0.144048399646862	0.7395820863823436
7	0.07571825068381592	0.45355014204334554
8	0.0440067650491097	0.3012562924660223
9	0.03065717052431349	0.23610287439363073
>10	0.09394630327156925	1.2284202057863611
>50	0.0023019185001330353	0.13016330481342323
>100	4.5067176471547E-4	0.05387973911634676
>500	4.68589699866237E-5	0.02742546979787409
>1k	1.5614967287177324E-5	0.01845276507357033
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.514014195075306E-6	0.0	0.0	0.0	8.514014195075306E-6
2	8.514014195075306E-6	0.0	0.0	0.0	8.514014195075306E-6
3	8.514014195075306E-6	0.0	0.0	0.0	8.514014195075306E-6
4	8.514014195075306E-6	8.514014195075306E-6	0.0	0.0	1.7028028390150613E-5
5	8.514014195075306E-6	8.514014195075306E-6	0.0	8.514014195075306E-6	1.7028028390150613E-5
6	8.514014195075306E-6	8.514014195075306E-6	0.0	8.514014195075306E-6	1.7028028390150613E-5
7	8.514014195075306E-6	8.514014195075306E-6	0.0	1.7028028390150613E-5	1.7028028390150613E-5
8	8.514014195075306E-6	8.514014195075306E-6	0.0	3.4056056780301226E-5	2.554204258522592E-5
9	8.514014195075306E-6	3.4056056780301226E-5	0.0	5.108408517045184E-5	4.257007097537654E-5
10-11	5.108408517045184E-5	3.4056056780301226E-5	0.0	1.0642517743844134E-4	5.108408517045184E-5
12-13	5.9598099365527146E-5	3.4056056780301226E-5	0.0	1.1919619873105429E-4	5.108408517045184E-5
14-15	6.38551064630648E-5	9.791116324336603E-5	0.0	1.1919619873105429E-4	8.514014195075306E-5
16-17	7.23691206581401E-5	1.0216817034090368E-4	0.0	1.362242271212049E-4	9.365415614582837E-5
18-19	7.662612775567776E-5	1.0642517743844134E-4	0.0	1.4899524841381786E-4	1.0642517743844134E-4
20-21	7.662612775567776E-5	1.1919619873105429E-4	0.0	2.0007933358426972E-4	1.2345320582859195E-4
22-23	8.939714904829073E-5	1.4899524841381786E-4	0.0	2.1710736197442035E-4	1.362242271212049E-4
24-25	1.4048123421874257E-4	1.575092626088932E-4	0.0	2.469064116571839E-4	1.5325225551135552E-4
26-27	1.745372909990438E-4	1.6176626970643084E-4	0.0	2.8096246843748514E-4	1.6176626970643084E-4
28-29	1.7879429809658143E-4	1.6602327680396848E-4	0.0	5.53410922679895E-4	1.6176626970643084E-4
30-31	1.7879429809658143E-4	1.9156531938919439E-4	0.0	0.0015367795622110928	1.6176626970643084E-4
32-33	1.7879429809658143E-4	2.0433634068180737E-4	0.0	0.0035631149406390155	1.7879429809658143E-4
34-35	2.0007933358426972E-4	2.639344400473345E-4	0.0	0.0059172398655773385	1.7879429809658143E-4
36-37	2.21364369071958E-4	3.2353253941286167E-4	0.0	0.00994436857984796	1.7879429809658143E-4
38-39	2.21364369071958E-4	3.490745819980876E-4	0.0	0.016206426020325845	1.830513051941191E-4
40-41	2.469064116571839E-4	3.661026103882382E-4	0.0	0.023226230724165436	1.9582232648673206E-4
42-43	2.469064116571839E-4	3.7887363168085113E-4	0.0	0.02968411049113006	1.9582232648673206E-4
44-45	2.639344400473345E-4	4.2144370265622766E-4	0.0	0.03700190569179729	1.9582232648673206E-4
46-47	2.8096246843748514E-4	4.5124275233899126E-4	0.0	0.044664518467365064	2.0433634068180737E-4
48-49	2.8096246843748514E-4	4.5975676653406655E-4	0.0	0.052476126491346656	2.469064116571839E-4
50-51	2.8096246843748514E-4	5.363828942897443E-4	0.0	0.06018982335208488	2.554204258522592E-4
52-53	2.9799049682763577E-4	5.448969084848196E-4	0.0	0.06779283802828713	2.596774329497968E-4
54-55	2.9799049682763577E-4	5.491539155823573E-4	0.0	0.07499995104441838	2.639344400473345E-4
56-57	2.9799049682763577E-4	5.704389510700456E-4	0.0	0.08196441465598997	2.9373348973009807E-4
58-59	3.0650451102271105E-4	5.746959581675832E-4	0.0	0.08842655143005214	3.277895465103993E-4
60-61	3.2353253941286167E-4	5.832099723626585E-4	0.0	0.09473543594860294	3.405605678030123E-4
62-63	3.2353253941286167E-4	6.002380007528092E-4	0.0	0.1006782178567655	3.490745819980876E-4
64-65	3.2353253941286167E-4	7.151771923863257E-4	0.0	0.10656140166556255	3.490745819980876E-4
66-67	3.2353253941286167E-4	7.236912065814011E-4	0.0	0.11269149188601676	3.490745819980876E-4
68-69	3.2353253941286167E-4	7.49233249166627E-4	0.0	0.11908551654651832	3.533315890956252E-4
70-71	3.277895465103993E-4	7.577472633617023E-4	0.0	0.12532628895150852	3.661026103882382E-4
72-73	3.3204655360793696E-4	7.577472633617023E-4	0.0	0.13019204806399404	4.3421472394884064E-4
74-75	3.3204655360793696E-4	7.747752917518528E-4	0.0	0.13420214874987452	4.5124275233899126E-4
76-77	3.3204655360793696E-4	8.045743414346164E-4	0.0	0.13766309552017264	4.5124275233899126E-4
78-79	3.3204655360793696E-4	8.173453627272295E-4	0.0	0.14083882281493573	4.5124275233899126E-4
80-81	3.3204655360793696E-4	8.173453627272295E-4	0.0	0.14241817244812222	4.7678479492421717E-4
82-83	3.3204655360793696E-4	8.216023698247671E-4	0.0	0.14295881234950947	5.193548658995938E-4
84-85	3.4056056780301224E-4	8.258593769223048E-4	0.0	0.14346965320121402	5.193548658995938E-4
86-87	3.490745819980876E-4	8.514014195075307E-4	0.0	0.14370804559867612	5.236118729971314E-4
88-89	3.490745819980876E-4	9.109995188730578E-4	0.0	0.14375912968384655	5.321258871922067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14065	0.0	47.66189	1
GTATCAA	24390	0.0	35.154705	1
TCAACGC	29255	0.0	28.41085	4
ATCAACG	29265	0.0	28.358242	3
CAACGCA	29715	0.0	27.942436	5
TATCAAC	31195	0.0	27.133186	2
AACGCAG	30990	0.0	26.874868	6
ACGCAGA	36070	0.0	23.089788	7
CGCAGAG	36645	0.0	22.739033	8
GTACATG	40245	0.0	21.578499	1
TACATGG	39565	0.0	21.264786	2
ACATGGG	40170	0.0	20.343735	3
GTGGTAT	7080	0.0	19.964985	1
TGGTATC	6735	0.0	19.228245	2
GCAGAGT	44190	0.0	18.789534	9
GAGTACT	22795	0.0	17.803833	12-13
AGAGTAC	39460	0.0	17.62592	10-11
CATGGGG	26695	0.0	17.565184	4
CAGAGTA	43120	0.0	17.194696	10-11
AGTACTT	25555	0.0	15.392461	12-13
>>END_MODULE