FastQCFastQC Report
Fri 27 May 2016
SRR2139987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10708560
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105750.039.712721
GTATCAA178650.034.3095741
TCAACGC206150.028.9078454
ATCAACG208450.028.6088713
CAACGCA211300.028.3037245
AACGCAG221850.027.091876
TATCAAC224550.026.8042262
ACGCAGA262900.022.8615577
CGCAGAG267550.022.4324518
CTTATAC78250.021.0765951
TACATGG262100.020.9531122
GTACATG269400.020.6535681
ACATGGG269850.019.8469163
GCAGAGT323750.018.380929
CATGGGG204950.017.815434
TATACAC123650.017.5315763
AGAGTAC281800.017.26329810-11
GAGTACT178200.017.03089512-13
CAGAGTA313900.016.1411310-11
CTAACGC7500.015.8687133
Produced by FastQC (version 0.11.5)