##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10708560
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93078658568472	31.0	31.0	34.0	30.0	34.0
2	32.161437298759125	33.0	31.0	34.0	30.0	34.0
3	32.25228079218868	34.0	31.0	34.0	30.0	34.0
4	35.857156797926145	37.0	35.0	37.0	35.0	37.0
5	35.518772178518866	37.0	35.0	37.0	33.0	37.0
6	35.51413224560538	37.0	35.0	37.0	33.0	37.0
7	35.53030024578468	37.0	35.0	37.0	33.0	37.0
8	35.49626541757248	37.0	35.0	37.0	33.0	37.0
9	37.28508165430273	39.0	37.0	39.0	34.0	39.0
10-11	37.090267692388146	39.0	37.0	39.0	33.0	39.0
12-13	37.12982562548092	39.0	37.0	39.0	33.0	39.0
14-15	38.27627421427344	40.0	38.0	41.0	34.0	41.0
16-17	38.2018621551357	40.0	38.0	41.0	33.5	41.0
18-19	38.22519400367557	40.0	38.0	41.0	33.5	41.0
20-21	38.260690361697044	40.0	38.0	41.0	34.0	41.0
22-23	38.206633177252385	40.0	38.0	41.0	34.0	41.0
24-25	38.17775372094715	40.0	38.0	41.0	34.0	41.0
26-27	38.04687462556769	40.0	37.5	41.0	33.5	41.0
28-29	37.94625409173526	40.0	37.0	41.0	33.0	41.0
30-31	37.805281901797414	39.0	37.0	40.5	33.0	41.0
32-33	37.72665090392941	39.0	37.0	40.0	33.0	41.0
34-35	37.64612425787321	39.0	37.0	40.0	33.0	41.0
36-37	37.56978218990635	39.0	37.0	40.0	33.0	41.0
38-39	37.474658202711915	39.0	37.0	40.0	32.5	41.0
40-41	37.44197622321491	39.0	37.0	40.0	32.0	41.0
42-43	37.37732058706768	39.0	36.0	40.0	32.0	41.0
44-45	37.3230214523252	39.0	36.0	40.0	32.0	41.0
46-47	37.212945446750396	39.0	36.0	40.0	31.5	41.0
48-49	37.19088618586656	39.0	36.0	40.0	31.0	41.0
50-51	37.1066804088737	39.0	36.0	40.0	31.0	41.0
52-53	36.902959084714766	39.0	35.5	40.0	31.0	41.0
54-55	36.7269233153599	38.5	35.0	40.0	31.0	41.0
56-57	36.545286880796034	38.0	35.0	40.0	30.0	41.0
58-59	36.31604353224971	38.0	35.0	40.0	30.0	41.0
60-61	36.14227201999521	38.0	34.0	40.0	30.0	41.0
62-63	35.98190874636026	38.0	34.0	40.0	29.5	41.0
64-65	35.71156785354205	37.0	34.0	40.0	29.0	41.0
66-67	35.51861888193435	37.0	34.0	39.0	29.0	41.0
68-69	35.2471661680551	36.0	34.0	39.0	29.0	40.0
70-71	34.9402018590914	36.0	33.5	39.0	29.0	40.0
72-73	34.70379582671271	35.5	33.0	38.5	29.0	40.0
74-75	34.3573952862002	35.0	33.0	37.5	28.5	39.5
76-77	32.30156775105718	33.5	30.0	35.5	25.5	38.5
78-79	33.57883806812458	35.0	32.0	36.0	28.0	39.0
80-81	33.62541752426177	35.0	33.0	36.0	29.0	39.0
82-83	33.40806122629144	35.0	32.5	36.0	28.5	37.5
84-85	33.14248556097897	34.5	32.0	35.5	28.0	37.0
86-87	32.91047894858524	34.0	32.0	35.0	27.0	37.0
88-89	32.709122461614115	34.0	32.0	35.0	27.0	36.0
90-91	32.68721552749635	34.0	32.0	35.0	27.0	36.0
92-93	32.61996307340764	34.0	32.0	35.0	27.0	36.0
94-95	32.54846612907218	34.0	32.0	35.0	27.0	35.0
96-97	32.61176044765948	34.0	32.0	35.0	28.0	35.0
98-99	32.77185070509472	34.0	32.0	35.0	29.0	35.0
100-101	31.54009298767111	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	37.0
20	135.0
21	544.0
22	1957.0
23	6074.0
24	15227.0
25	31288.0
26	56141.0
27	90264.0
28	133791.0
29	184935.0
30	246364.0
31	325251.0
32	430451.0
33	586264.0
34	834939.0
35	1231060.0
36	1860516.0
37	2411390.0
38	1820069.0
39	438715.0
40	3142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43291908529251	20.590163383312042	14.004459983415138	23.97245754798031
2	14.947486262232879	21.226853295850113	41.430728072708284	22.394932369208725
3	18.78985763173785	25.870450054938043	30.131384388331206	25.208307924992894
4	12.914857477597058	17.97997054123317	38.42215647399498	30.683015507174797
5	13.26653754253957	37.88327635865853	35.630863125468146	13.21932297333376
6	30.373773476404487	38.004063680894035	18.433960703059004	13.18820213964248
7	26.861352039863434	32.257941310503	22.94060079039572	17.940105859237843
8	24.281478821096066	37.52176880601186	20.971714489636653	17.22503788325542
9	25.390158993974797	16.842786570206396	21.001849088562107	36.7652053472567
10-11	24.372450887049634	26.766501335457253	28.799245496885213	20.061802280607903
12-13	25.01190053686538	24.06381237124483	29.03386877617097	21.890418315718826
14-15	22.066524833059916	25.604176846414656	26.334854157527754	25.994444162997677
16-17	21.95309549663427	28.87094430567649	26.993155172458877	22.18280502523037
18-19	22.033023114218906	28.141262690781954	28.41525844744765	21.41045574755149
20-21	22.63217164463765	27.332525180682165	28.35271011692656	21.68259305775362
22-23	22.35695239691052	27.334221291579965	28.23520890345563	22.073617408053895
24-25	22.18357261712642	27.40562766257456	28.346925445039457	22.063874275259565
26-27	22.206384149851623	27.51861569359027	28.259182273173238	22.015817883384866
28-29	22.053278190001215	27.604182815728574	28.30619028237668	22.036348711893535
30-31	22.393745722057666	27.420471360531053	28.11672769216664	22.06905522524464
32-33	22.24938804179474	27.568213926606305	28.185816645732388	21.996581385866566
34-35	22.285759634485146	27.70414273723205	28.166573185789257	21.843524442493546
36-37	22.263984662788424	27.523757597950784	28.134270666128685	22.077987073132107
38-39	22.162595313355656	27.610635784065902	28.24568554297635	21.981083359602096
40-41	22.364246168018493	27.49372882484372	28.159498806516158	21.98252620062163
42-43	22.295379106937943	27.531954395525915	28.028081820905964	22.144584676630174
44-45	22.269209267379512	27.716534738768424	27.871119614159408	22.143136379692663
46-47	22.456119013549404	27.548060547020885	27.615529897887715	22.380290541541996
48-49	22.414157029212358	27.623220357522914	27.66765501308233	22.294967600182396
50-51	22.273671749583745	27.78001410102251	27.644883032391316	22.301431117002426
52-53	22.428243770022828	27.724271581176684	27.596310025605852	22.25117462319464
54-55	22.39368384818843	27.64824659652001	27.5554768148354	22.40259274045616
56-57	22.501865898498693	27.828153337847105	27.41413741554727	22.255843348106932
58-59	22.3565138538046	27.94136152136729	27.544663271023772	22.157461353804344
60-61	22.487541834113735	28.00087656766501	27.339146059648762	22.17243553857249
62-63	22.41789735873537	28.03783927165439	27.331418833853572	22.212844535756666
64-65	22.358127315531473	27.96015247926537	27.372471534486547	22.30924867071661
66-67	22.45915191231182	27.8905930819747	27.282967905020232	22.367287100693247
68-69	22.4005351833337	27.84763354391374	27.389093548167182	22.362737724585376
70-71	22.427464373354784	27.610601978988914	27.41287117302701	22.5490624746293
72-73	22.444110790509335	27.684664039905616	27.406625265215723	22.464599904369326
74-75	22.319586063087836	27.84695748025961	27.367644022700212	22.465812433952344
76-77	22.409668283967374	27.818181591070058	27.2837762599394	22.488373865023163
78-79	22.41460477768025	27.816259073711585	27.22880294329807	22.540333205310095
80-81	22.37511259548818	27.834308037074084	27.31401457498313	22.476564792454603
82-83	22.480956066010492	27.73832647941682	27.39173700742823	22.38898044714446
84-85	22.53630499739501	27.757524032507696	27.314938093777013	22.391232876320277
86-87	22.316256984816416	27.80574224996591	27.40733486453394	22.470665900683738
88-89	22.4079818521181	27.780968708581177	27.335777178483323	22.475272260817402
90-91	22.398393199854482	27.81257400781678	27.389606400561657	22.39942639176708
92-93	22.41337778423291	27.80335233832461	27.373806555990406	22.409463321452073
94-95	22.232283471178686	27.897009929438628	27.42043873074018	22.450267868642506
96-97	22.214105267227133	27.917132567821216	27.494723611504234	22.374038553447413
98-99	22.435177224911556	28.554045341486695	27.520487587573733	21.490289846028016
100-101	23.25541169160495	31.159516252468038	24.533969565457298	21.051102490469713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1404.0
1	1313.0
2	2104.0
3	3190.0
4	4449.5
5	7271.0
6	9613.5
7	11314.5
8	12451.0
9	12037.5
10	10673.0
11	9447.5
12	10051.5
13	13331.5
14	21364.5
15	32155.0
16	40469.0
17	45105.0
18	46282.0
19	44732.5
20	42117.0
21	40144.5
22	40831.5
23	46401.5
24	57127.5
25	72600.0
26	92748.5
27	114457.5
28	136261.0
29	158828.5
30	179106.5
31	199130.5
32	221919.5
33	244110.0
34	265963.5
35	287599.0
36	310805.5
37	332025.0
38	348911.5
39	365157.5
40	382646.5
41	399263.5
42	411438.5
43	423253.5
44	436623.0
45	447935.0
46	455311.5
47	463224.5
48	472137.5
49	471746.5
50	462117.0
51	449498.5
52	428975.5
53	402052.5
54	374888.5
55	346563.5
56	317167.5
57	285624.0
58	253111.0
59	220428.0
60	185036.0
61	151118.0
62	121362.5
63	94846.5
64	71617.0
65	53482.5
66	40072.0
67	30440.0
68	23362.0
69	17260.5
70	12612.5
71	9230.5
72	6665.5
73	4679.0
74	3299.5
75	2404.5
76	1652.0
77	1114.0
78	790.5
79	557.0
80	409.0
81	301.0
82	219.5
83	161.0
84	121.0
85	84.0
86	54.0
87	37.5
88	26.5
89	18.5
90	12.5
91	7.5
92	4.5
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011579521429585305
3	0.0012513353802938957
4	3.0816468320670566E-4
5	9.338323733536536E-6
6	3.9220959680853453E-4
7	0.0
8	2.8014971200609602E-5
9	3.8287127307499796E-4
10-11	5.182769672112777E-4
12-13	8.871407546859709E-5
14-15	2.3345809333841337E-5
16-17	3.3617965440731526E-4
18-19	0.0
20-21	3.735537225585684E-5
22-23	1.8680090054846146E-5
24-25	0.0
26-27	6.075385533450863E-5
28-29	2.33832023772673E-4
30-31	6.133884340319703E-4
32-33	3.190571223919074E-4
34-35	9.408370165926486E-4
36-37	1.085909358201602E-4
38-39	9.967958235584053E-5
40-41	2.0059314437586494E-4
42-43	0.0012114944866183945
44-45	3.197595873008352E-4
46-47	1.4665103301476494E-4
48-49	3.896031201785073E-4
50-51	0.0
52-53	3.7609062520402916E-4
54-55	6.117873833565416E-4
56-57	6.225150741770083E-4
58-59	5.771247336595119E-4
60-61	5.517556996493904E-4
62-63	4.732956845898659E-5
64-65	5.739142578475601E-4
66-67	0.002181325298032971
68-69	0.0010212488754718998
70-71	1.867609758326903E-4
72-73	0.0
74-75	3.368881962966667E-5
76-77	5.67779372291515E-6
78-79	9.014561445030754E-4
80-81	1.6276037882245658E-4
82-83	4.710053798234483E-5
84-85	7.333747083866645E-4
86-87	1.7520348676688065E-4
88-89	1.2255787351304362E-5
90-91	6.224047278858978E-6
92-93	5.0672631679959254E-5
94-95	3.294170809732376E-4
96-97	1.98584397740189E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	378.0
20-21	1125.0
22-23	2409.0
24-25	4437.0
26-27	6394.0
28-29	10609.0
30-31	21014.0
32-33	25637.0
34-35	34390.0
36-37	52382.0
38-39	64620.0
40-41	66824.0
42-43	76099.0
44-45	90699.0
46-47	91170.0
48-49	87225.0
50-51	82252.0
52-53	80294.0
54-55	88121.0
56-57	94873.0
58-59	97073.0
60-61	98872.0
62-63	98268.0
64-65	100350.0
66-67	103629.0
68-69	101162.0
70-71	101528.0
72-73	97225.0
74-75	100182.0
76-77	95471.0
78-79	104936.0
80-81	109723.0
82-83	106452.0
84-85	108368.0
86-87	114750.0
88-89	123062.0
90-91	137205.0
92-93	147534.0
94-95	173938.0
96-97	269820.0
98-99	966970.0
100-101	6371090.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.0491240295843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69047487284755	76.28096321733126
2	7.0806012512646745	12.043978680456783
3	1.7962175054138108	4.5830017620614925
4	0.6697978805715761	2.278628920779387
5	0.31893703065254014	1.356265753879761
6	0.15673078775900048	0.7997889724421812
7	0.09237835669242617	0.5499688821188332
8	0.05541478962568704	0.3770383452758704
9	0.032804999650624014	0.25110328356687533
>10	0.10475885139449065	1.3151386848200683
>50	0.0015554547829081033	0.08834394538534159
>100	2.5848375268918675E-4	0.03739331499357217
>500	6.973559197881998E-5	0.0383862368885476
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.338323733536536E-6
2	0.0	4.669161866768267E-5	0.0	0.0	9.338323733536536E-6
3	0.0	4.669161866768267E-5	0.0	9.338323733536536E-6	9.338323733536536E-6
4	0.0	7.470658986829229E-5	0.0	9.338323733536536E-6	9.338323733536536E-6
5	0.0	7.470658986829229E-5	0.0	9.338323733536536E-6	9.338323733536536E-6
6	0.0	7.470658986829229E-5	0.0	9.338323733536536E-6	1.867664746707307E-5
7	0.0	7.470658986829229E-5	0.0	3.735329493414614E-5	1.867664746707307E-5
8	0.0	9.338323733536535E-5	0.0	3.735329493414614E-5	1.867664746707307E-5
9	0.0	1.4007485600304803E-4	0.0	7.470658986829229E-5	1.867664746707307E-5
10-11	0.0	1.4007485600304803E-4	0.0	1.0739072293567016E-4	1.867664746707307E-5
12-13	0.0	1.4007485600304803E-4	0.0	1.1205988480243842E-4	2.334580933384134E-5
14-15	0.0	1.7742815093719415E-4	0.0	1.1205988480243842E-4	2.8014971200609605E-5
16-17	0.0	1.867664746707307E-4	0.0	1.1205988480243842E-4	2.8014971200609605E-5
18-19	0.0	1.9610479840426724E-4	0.0	1.3540569413627977E-4	2.8014971200609605E-5
20-21	0.0	2.0544312213780378E-4	0.0	1.5408234160335283E-4	2.8014971200609605E-5
22-23	0.0	2.4279641707194993E-4	0.0	1.587515034701211E-4	4.202245680091441E-5
24-25	0.0	2.7081138827255954E-4	0.0	1.7742815093719418E-4	5.602994240121921E-5
26-27	0.0	2.7081138827255954E-4	0.0	2.1478144587134033E-4	6.536826613475574E-5
28-29	0.0	2.8948803573963257E-4	0.0	5.322844528115826E-4	6.536826613475574E-5
30-31	0.0	3.5485630187438835E-4	0.0	0.00142876353123109	7.003742800152401E-5
32-33	0.0	3.6419462560792487E-4	0.0	0.0033524582203396163	8.404491360182882E-5
34-35	9.338323733536536E-6	4.2489372987591234E-4	0.0	0.005915828085195396	9.338323733536535E-5
36-37	9.338323733536536E-6	4.762545104103633E-4	0.0	0.009772555787145984	9.338323733536535E-5
38-39	9.338323733536536E-6	4.996003197442046E-4	0.0	0.015823789566477658	9.338323733536535E-5
40-41	9.338323733536536E-6	5.556302621454238E-4	0.0	0.022524036845290123	9.805239920213362E-5
42-43	9.338323733536536E-6	5.74306909612497E-4	0.0	0.029014171840098017	1.1205988480243842E-4
44-45	9.338323733536536E-6	6.396751757472526E-4	0.0	0.03627471854292267	1.2139820853597495E-4
46-47	9.338323733536536E-6	6.910359562817036E-4	0.0	0.0443196844393644	1.2606737040274322E-4
48-49	9.338323733536536E-6	6.910359562817036E-4	0.0	0.05194442576779698	1.3073653226951148E-4
50-51	9.338323733536536E-6	7.84419193617069E-4	0.0	0.059335709002891146	1.3073653226951148E-4
52-53	9.338323733536536E-6	7.937575173506055E-4	0.0	0.06683438296092098	1.4474401786981629E-4
54-55	9.338323733536536E-6	8.21772488551215E-4	0.0	0.07459452998348984	1.587515034701211E-4
56-57	9.338323733536536E-6	8.497874597518247E-4	0.0	0.08248541353832822	1.587515034701211E-4
58-59	9.338323733536536E-6	8.778024309524343E-4	0.0	0.09054905608223701	1.587515034701211E-4
60-61	9.338323733536536E-6	8.964790784195074E-4	0.0	0.0981597899250693	1.587515034701211E-4
62-63	9.338323733536536E-6	9.011482402862756E-4	0.0	0.10554173483642992	1.6808982720365763E-4
64-65	9.338323733536536E-6	0.0010085389632219458	0.0	0.11263419171205091	1.6808982720365763E-4
66-67	9.338323733536536E-6	0.001031884772555787	0.0	0.11990407673860912	1.6808982720365763E-4
68-69	9.338323733536536E-6	0.0010505614200228602	0.0	0.12715061595583346	1.6808982720365763E-4
70-71	9.338323733536536E-6	0.0010692380674899335	0.0	0.13344464615223708	1.6808982720365763E-4
72-73	9.338323733536536E-6	0.0010739072293567016	0.0	0.13789435741126727	1.7742815093719418E-4
74-75	9.338323733536536E-6	0.0011112605242908477	0.0	0.14170906265641692	1.7742815093719418E-4
76-77	9.338323733536536E-6	0.001153282981091762	0.0	0.14498681428688825	1.7742815093719418E-4
78-79	9.338323733536536E-6	0.0011626213048252987	0.0	0.147993754529087	1.7742815093719418E-4
80-81	9.338323733536536E-6	0.0011766287904256035	0.0	0.14949255548831963	1.8209731280396243E-4
82-83	9.338323733536536E-6	0.0011906362760259084	0.0	0.15020226809206838	2.0544312213780378E-4
84-85	9.338323733536536E-6	0.001204643761626213	0.0	0.15083260494408213	2.0544312213780378E-4
86-87	9.338323733536536E-6	0.0012139820853597496	0.0	0.1510707321992873	2.0544312213780378E-4
88-89	9.338323733536536E-6	0.0012233204090932861	0.0	0.15113143130355527	2.0544312213780378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10575	0.0	39.71272	1
GTATCAA	17865	0.0	34.309574	1
TCAACGC	20615	0.0	28.907845	4
ATCAACG	20845	0.0	28.608871	3
CAACGCA	21130	0.0	28.303724	5
AACGCAG	22185	0.0	27.09187	6
TATCAAC	22455	0.0	26.804226	2
ACGCAGA	26290	0.0	22.861557	7
CGCAGAG	26755	0.0	22.432451	8
CTTATAC	7825	0.0	21.076595	1
TACATGG	26210	0.0	20.953112	2
GTACATG	26940	0.0	20.653568	1
ACATGGG	26985	0.0	19.846916	3
GCAGAGT	32375	0.0	18.38092	9
CATGGGG	20495	0.0	17.81543	4
TATACAC	12365	0.0	17.531576	3
AGAGTAC	28180	0.0	17.263298	10-11
GAGTACT	17820	0.0	17.030895	12-13
CAGAGTA	31390	0.0	16.14113	10-11
CTAACGC	750	0.0	15.868713	3
>>END_MODULE