FastQCFastQC Report
Fri 27 May 2016
SRR2139986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9142110
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138750.045.5649031
CTTATAC93150.037.2865681
GTATCAA252550.034.5618361
TATACAC121200.031.5566083
ATCAACG307400.027.7489513
TCAACGC308850.027.618224
CAACGCA309600.027.5514685
TATCAAC317050.027.13942
AACGCAG320000.026.759416
TTATACA142150.025.4517212
ACGCAGA366600.023.3126757
TACACAT164300.023.0517735
CGCAGAG371550.022.9798328
ACACATC155700.022.5989046
GTACATG345550.022.3163871
TACATGG339200.022.3075162
ACATCTC166750.021.3987798
ACATGGG346100.021.396623
GCAGAGT423750.020.0904989
CACATCT177100.019.6812697
Produced by FastQC (version 0.11.5)