##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9142110
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.479583050302395	34.0	31.0	34.0	31.0	34.0
2	32.68208794249905	34.0	31.0	34.0	31.0	34.0
3	32.77301837322019	34.0	31.0	34.0	31.0	34.0
4	36.19376413103758	37.0	37.0	37.0	35.0	37.0
5	36.037632340892856	37.0	35.0	37.0	35.0	37.0
6	36.06447144040052	37.0	35.0	37.0	35.0	37.0
7	36.08523087121026	37.0	35.0	37.0	35.0	37.0
8	36.07855735710903	37.0	35.0	37.0	35.0	37.0
9	37.94781303222123	39.0	38.0	39.0	35.0	39.0
10-11	37.83511897144095	39.0	38.0	39.0	35.0	39.0
12-13	37.85970197252057	39.0	38.0	39.0	35.0	39.0
14-15	39.16393622478837	40.0	39.0	41.0	36.0	41.0
16-17	39.13504989548365	40.0	39.0	41.0	36.0	41.0
18-19	39.178505399738135	40.0	39.0	41.0	36.0	41.0
20-21	39.22524506965695	40.0	39.0	41.0	36.0	41.0
22-23	39.183798109987926	40.0	39.0	41.0	36.0	41.0
24-25	39.16453103312076	40.0	39.0	41.0	36.0	41.0
26-27	39.05655641393625	40.0	39.0	41.0	36.0	41.0
28-29	38.97817956481673	40.0	38.5	41.0	35.0	41.0
30-31	38.84785354077105	40.0	38.0	41.0	35.0	41.0
32-33	38.76556764526999	40.0	38.0	41.0	35.0	41.0
34-35	38.702239701087194	40.0	38.0	41.0	35.0	41.0
36-37	38.63494621371116	40.0	38.0	41.0	35.0	41.0
38-39	38.5475292072756	40.0	38.0	41.0	35.0	41.0
40-41	38.54633145847129	40.0	38.0	41.0	35.0	41.0
42-43	38.49612032311239	40.0	38.0	41.0	34.0	41.0
44-45	38.45962047847814	40.0	38.0	41.0	34.0	41.0
46-47	38.35794281661347	40.0	38.0	41.0	34.0	41.0
48-49	38.339858888212866	40.0	38.0	41.0	34.0	41.0
50-51	38.25926914545522	40.0	37.5	41.0	34.0	41.0
52-53	38.08109065073194	40.0	37.0	41.0	33.5	41.0
54-55	37.932988228552034	40.0	37.0	41.0	33.0	41.0
56-57	37.73422553781555	39.0	36.0	41.0	33.0	41.0
58-59	37.49606073889473	39.0	36.0	41.0	33.0	41.0
60-61	37.327398706482285	39.0	35.5	40.5	32.5	41.0
62-63	37.15133694657081	39.0	35.0	40.0	32.0	41.0
64-65	36.88676338916458	38.0	35.0	40.0	32.0	41.0
66-67	36.68344710279077	37.5	35.0	40.0	32.0	41.0
68-69	36.40339999583357	37.0	35.0	40.0	32.0	41.0
70-71	36.07617487380452	36.5	35.0	39.0	31.0	41.0
72-73	35.82278961008894	36.0	35.0	39.0	31.0	41.0
74-75	35.47553236243909	35.5	34.0	38.5	31.0	40.0
76-77	33.498936835210195	34.5	32.0	36.5	28.0	39.0
78-79	34.63631976666626	35.0	34.0	37.0	30.5	39.0
80-81	34.628503476664136	35.0	34.0	36.5	31.0	39.0
82-83	34.40608703858383	35.0	34.0	36.0	31.0	38.0
84-85	34.15425050481163	35.0	34.0	36.0	31.0	37.0
86-87	33.953339256907654	35.0	34.0	35.5	30.0	37.0
88-89	33.76366228479198	35.0	34.0	35.0	30.0	36.5
90-91	33.7212359353518	35.0	34.0	35.0	30.0	36.0
92-93	33.64679505007949	35.0	34.0	35.0	30.0	36.0
94-95	33.57088426378441	35.0	34.0	35.0	30.0	36.0
96-97	33.578728230205286	35.0	34.0	35.0	30.0	35.0
98-99	33.64238770971127	35.0	34.0	35.0	31.0	35.0
100-101	32.38496522950851	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	3.0
18	14.0
19	54.0
20	72.0
21	244.0
22	790.0
23	2449.0
24	6273.0
25	13244.0
26	24483.0
27	39935.0
28	60419.0
29	83793.0
30	112830.0
31	148165.0
32	198809.0
33	274013.0
34	413139.0
35	654003.0
36	1212906.0
37	2272625.0
38	2538484.0
39	1066362.0
40	18999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18020785136035	20.106189927708154	13.717369403780966	23.996232817150524
2	14.877390622253381	20.849758130650685	41.65780729407032	22.61504395302562
3	18.61327793422464	26.21212167618474	30.119633721815497	25.054966667775112
4	12.668195427630845	17.73573830972592	38.09493806855448	31.50112819408876
5	13.228555905426198	37.95054292177002	35.258844540138384	13.562056632665396
6	30.08334861066689	37.50734515237445	18.478696979813357	13.930609257145306
7	26.664522741467778	32.49837291391156	22.569702180350053	18.26740216427061
8	24.75313945748818	36.65189800379672	21.044912522881766	17.550050015833335
9	25.899958838585235	16.362094438921133	20.769122310219682	36.96882441227395
10-11	24.687083928931617	26.547543728618077	28.292542781348818	20.472829561101495
12-13	25.445256062092525	23.967974969424418	28.591300531748782	21.995468436734278
14-15	22.606476536921242	25.29084101828594	26.179334131690414	25.923348313102405
16-17	22.109757939756047	28.606568342397114	26.737438803473566	22.546234914373265
18-19	22.162580629635826	27.58031242240577	28.587147824736302	21.669959123222103
20-21	23.057533145498905	26.63710372829502	28.421775113028275	21.8835880131778
22-23	22.682108292493957	26.81649907631074	28.33600660686841	22.16538602432689
24-25	22.48972516660391	27.02457632691954	28.482968545151	22.00272996132555
26-27	22.472778820382526	27.189481830123285	28.371331450477832	21.966407899016353
28-29	22.158259729354732	27.3203002358658	28.39318942977624	22.12825060500323
30-31	22.86433102504878	27.040984731496952	28.282813760326743	21.811870483127525
32-33	22.109741964913145	27.458177833040683	28.340407165664832	22.091673036381344
34-35	22.365274083862115	27.343543317765256	28.499733319927977	21.791449278444656
36-37	22.252112695767146	27.310131720969327	28.395424141876656	22.042331441386867
38-39	22.164515665201495	27.059129587831265	28.596447266886628	22.17990748008061
40-41	22.387331607825516	26.91368072887203	28.53152643513101	22.167461228171444
42-43	22.453861927546136	26.991740097837287	28.292527892633245	22.261870081983336
44-45	22.26948224602645	27.33836671225434	28.079798126822126	22.312352914897083
46-47	22.700668627794236	26.99230423458041	27.590849493522008	22.716177644103343
48-49	22.547517034810273	27.151592193712766	27.59670598061718	22.704184790859777
50-51	22.32876380510191	27.33227610472963	27.676305030887004	22.66265505928146
52-53	22.57517465536305	27.310632896044407	27.662902320229776	22.451290128362768
54-55	22.459518613997677	27.2094005409979	27.516964518483277	22.814116326521138
56-57	22.801785520030855	27.465047369930478	27.114253997765008	22.61891311227366
58-59	22.411203442050915	27.792525438096906	27.476086817987134	22.32018430186504
60-61	22.695369613971376	27.938143878696053	27.047312011698466	22.319174495634105
62-63	22.51010999489293	28.020597234158434	27.042121249992928	22.427171520955707
64-65	22.504791685184784	27.830261345925916	27.119993233121313	22.544953735767983
66-67	22.692344534203688	27.70527207328296	26.936825382995412	22.66555800951794
68-69	22.614019358557048	27.565534462935616	27.167941422811836	22.652504755695503
70-71	22.645472035203614	27.256118622440482	27.229326051989005	22.869083290366895
72-73	22.730640874813176	27.276220931573707	27.231352361160315	22.761785832452798
74-75	22.483275057153605	27.4831593136045	27.208393889521528	22.825171739720368
76-77	22.6833913299445	27.38452798442425	27.081641883214658	22.85043880241659
78-79	22.639374611332595	27.45478577046135	27.05588867348932	22.849950944716735
80-81	22.642987776425908	27.413347107791864	27.074459724295057	22.869205391487167
82-83	22.750690033342412	27.208820650213816	27.265407064469354	22.775082251974414
84-85	22.86973462526246	27.252312683458964	27.116372112596608	22.761580578681976
86-87	22.63406517066416	27.302823665706693	27.21892761480501	22.844183548824134
88-89	22.770357969974977	27.22979173212935	27.13579416467339	22.86405613322228
90-91	22.79691920686455	27.133430059197668	27.283651015036604	22.78599971890118
92-93	22.856533600052547	27.11145660360115	27.221162545031873	22.810847251314424
94-95	22.66510648672125	27.177807234089958	27.27592258622833	22.881163692960463
96-97	22.70958061770211	27.140446803102975	27.33433136665935	22.815641212535567
98-99	22.891587545405322	27.8101655619628	27.33200405898823	21.966242833643644
100-101	27.221302119282107	34.77620329688389	27.93607447875843	10.06642010507557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1735.0
1	1662.5
2	2711.0
3	4058.0
4	5666.0
5	9750.0
6	13194.0
7	15844.0
8	17767.0
9	17038.5
10	14534.0
11	12280.0
12	13086.5
13	17825.0
14	29302.0
15	44871.0
16	56823.5
17	62790.0
18	62940.0
19	58257.0
20	51747.0
21	45351.0
22	41539.5
23	43261.0
24	50750.5
25	63224.0
26	80316.5
27	98227.5
28	114759.0
29	132269.5
30	147820.5
31	161758.5
32	178472.0
33	197293.0
34	215358.5
35	233132.5
36	253596.0
37	271675.0
38	283401.5
39	293179.5
40	307092.0
41	320754.5
42	331535.0
43	345915.0
44	360450.5
45	375020.0
46	395369.0
47	417259.5
48	424626.5
49	422089.0
50	419409.0
51	413100.0
52	398036.5
53	377523.5
54	355890.5
55	330126.5
56	303961.5
57	274888.0
58	245560.5
59	216043.0
60	181404.0
61	146721.5
62	116129.5
63	89952.5
64	67573.5
65	50027.5
66	37609.0
67	28814.0
68	21940.5
69	15710.5
70	11117.0
71	7947.0
72	5409.0
73	3602.0
74	2502.5
75	1737.0
76	1223.0
77	861.5
78	593.0
79	414.0
80	282.5
81	190.0
82	140.5
83	106.5
84	74.0
85	51.0
86	37.0
87	26.0
88	14.5
89	9.5
90	6.0
91	2.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0020892332295279754
3	0.002253309137606089
4	7.219339955436984E-4
5	1.0938393871874217E-5
6	2.843982406687297E-4
7	0.0
8	5.469196935937109E-5
9	5.797348752093334E-4
10-11	4.594125426187171E-4
12-13	1.2032233259061639E-4
14-15	2.1876787743748435E-5
16-17	5.141045119780882E-4
18-19	0.0
20-21	3.828591759175015E-5
22-23	0.0
24-25	0.0
26-27	3.284176509161211E-5
28-29	2.5199274217077724E-4
30-31	6.255722408354353E-4
32-33	3.577447922402623E-4
34-35	0.001093776835165566
36-37	1.3883584588265916E-4
38-39	1.2313436741201951E-4
40-41	2.8801677409692343E-4
42-43	0.001487889011004735
44-45	4.035141470618838E-4
46-47	1.8101605244513855E-4
48-49	2.423910350315939E-4
50-51	0.0
52-53	5.802520288421524E-4
54-55	6.047157936555785E-4
56-57	5.871760140452503E-4
58-59	5.946882321903237E-4
60-61	6.342928259768792E-4
62-63	1.9286518395770542E-5
64-65	6.646928330603097E-4
66-67	0.0024392859092963763
68-69	0.0011196845573709269
70-71	2.2429042156880003E-4
72-73	0.0
74-75	9.761494613119196E-5
76-77	1.4124842419726754E-5
78-79	9.875364315070493E-4
80-81	1.7416396579477767E-4
82-83	9.56845319256225E-5
84-85	6.34221155901633E-4
86-87	2.042550719182108E-4
88-89	1.5354059624880363E-5
90-91	3.899815608918379E-5
92-93	1.586604488694491E-4
94-95	6.458102254362045E-4
96-97	8.233239101167407E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	769.0
22-23	1655.0
24-25	3593.0
26-27	6057.0
28-29	12084.0
30-31	25869.0
32-33	31211.0
34-35	42134.0
36-37	65253.0
38-39	79897.0
40-41	80796.0
42-43	92215.0
44-45	109691.0
46-47	107721.0
48-49	100130.0
50-51	89559.0
52-53	84763.0
54-55	93543.0
56-57	101870.0
58-59	102804.0
60-61	107565.0
62-63	104654.0
64-65	105181.0
66-67	109657.0
68-69	101890.0
70-71	97419.0
72-73	90562.0
74-75	91412.0
76-77	90824.0
78-79	96443.0
80-81	98073.0
82-83	92788.0
84-85	91245.0
86-87	94784.0
88-89	100635.0
90-91	107937.0
92-93	107035.0
94-95	115697.0
96-97	169044.0
98-99	720466.0
100-101	5216955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.41065696294318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00390103247446	76.87292316411363
2	6.938627808547122	11.852655190987127
3	1.7248640260684784	4.419653089147676
4	0.6551908189743627	2.2384111313875636
5	0.2753222965350274	1.1757729111801472
6	0.13716399584733094	0.7029160198189767
7	0.07311474103746338	0.43713446459796457
8	0.05032781049582086	0.34388250863636527
9	0.029897445830049284	0.22982044408726643
>10	0.10859299224864759	1.4182738542144353
>50	0.002386379243380121	0.13484020408396938
>100	4.681425268634905E-4	0.06196190820837136
>500	1.0179323112786226E-4	0.05913810485103896
>1k	4.0716939913594E-5	0.05261700468547245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.281518161562265E-5	0.0	1.0938393871874217E-5	0.0
3	0.0	3.281518161562265E-5	0.0	1.0938393871874217E-5	0.0
4	0.0	1.0938393871874218E-4	0.0	2.1876787743748435E-5	0.0
5	0.0	1.0938393871874218E-4	0.0	2.1876787743748435E-5	0.0
6	0.0	1.0938393871874218E-4	0.0	2.1876787743748435E-5	3.281518161562265E-5
7	0.0	1.2032233259061639E-4	0.0	3.281518161562265E-5	3.281518161562265E-5
8	0.0	1.2032233259061639E-4	0.0	3.281518161562265E-5	3.281518161562265E-5
9	0.0	2.7345984679685544E-4	0.0	3.281518161562265E-5	4.375357548749687E-5
10-11	1.0938393871874217E-5	2.7345984679685544E-4	0.0	3.281518161562265E-5	4.375357548749687E-5
12-13	1.0938393871874217E-5	2.843982406687296E-4	0.0	7.109956016718242E-5	4.375357548749687E-5
14-15	1.0938393871874217E-5	4.156589671312202E-4	0.0	7.656875710311953E-5	4.922277242343398E-5
16-17	1.6407590807811325E-5	4.375357548749687E-4	0.0	8.203795403905663E-5	6.56303632312453E-5
18-19	2.1876787743748435E-5	4.484741487468429E-4	0.0	1.0938393871874218E-4	6.56303632312453E-5
20-21	2.1876787743748435E-5	4.594125426187171E-4	0.0	1.257915295265535E-4	6.56303632312453E-5
22-23	3.281518161562265E-5	5.086353150421511E-4	0.0	1.5860671114217617E-4	9.844554484686795E-5
24-25	4.9222772423433975E-5	5.414504966577737E-4	0.0	2.2423707437342144E-4	1.312607264624906E-4
26-27	7.109956016718242E-5	5.797348752093335E-4	0.0	3.336210130921636E-4	1.5860671114217617E-4
28-29	7.656875710311953E-5	5.797348752093335E-4	0.0	0.0010227398270202393	1.6407590807811326E-4
30-31	7.656875710311953E-5	7.438107832874468E-4	0.0	0.002575991756826378	1.6954510501405036E-4
32-33	7.656875710311953E-5	7.711567679671322E-4	0.0	0.005102760741229322	1.859526958218617E-4
34-35	8.750715097499374E-5	9.735170545968053E-4	0.0	0.008581169992485323	1.859526958218617E-4
36-37	9.844554484686795E-5	0.001159469750418667	0.0	0.01397926736825525	1.859526958218617E-4
38-39	9.844554484686795E-5	0.0011922849320342896	0.0	0.022046332848762486	1.859526958218617E-4
40-41	1.0938393871874218E-4	0.001301668870753032	0.0	0.031201768519521205	1.859526958218617E-4
42-43	1.0938393871874218E-4	0.0013344840523686545	0.0	0.03936181034793937	1.859526958218617E-4
44-45	1.0938393871874218E-4	0.0014438679910873968	0.0	0.04824378617190124	1.859526958218617E-4
46-47	1.0938393871874218E-4	0.0015204367481905163	0.0	0.058947004575530156	2.0236028662967302E-4
48-49	1.0938393871874218E-4	0.0015204367481905163	0.0	0.06861107556133103	2.2970627130935856E-4
50-51	1.0938393871874218E-4	0.0016735742623967553	0.0	0.07830249253181158	2.3517546824529568E-4
52-53	1.0938393871874218E-4	0.0016899818532045666	0.0	0.08703680003850314	2.679906498609183E-4
54-55	1.0938393871874218E-4	0.0017063894440123779	0.0	0.09692510809867744	3.0080583147654096E-4
56-57	1.0938393871874218E-4	0.0017282662317561262	0.0	0.10586177589199867	3.062750284124781E-4
58-59	1.0938393871874218E-4	0.001766550610307686	0.0	0.11375382707055592	3.062750284124781E-4
60-61	1.0938393871874218E-4	0.0018376501704748685	0.0	0.12172244700621629	3.062750284124781E-4
62-63	1.0938393871874218E-4	0.001870465352090491	0.0	0.1288542798106783	3.062750284124781E-4
64-65	1.0938393871874218E-4	0.0021001716233998496	0.0	0.13573999875302312	3.062750284124781E-4
66-67	1.2032233259061639E-4	0.0021384560019514095	0.0	0.14284448557280538	3.117442253484152E-4
68-69	1.312607264624906E-4	0.002209555562118592	0.0	0.15015680187615332	3.172134222843523E-4
70-71	1.312607264624906E-4	0.002236901546798277	0.0	0.15702064403075439	3.2815181615622653E-4
72-73	1.312607264624906E-4	0.0022697167284139	0.0	0.16224919630151027	4.156589671312203E-4
74-75	1.312607264624906E-4	0.0023408162885810825	0.0	0.16689254450012087	4.156589671312203E-4
76-77	1.312607264624906E-4	0.002461138621171699	0.0	0.17064441359817373	4.156589671312203E-4
78-79	1.312607264624906E-4	0.0025322381813388814	0.0	0.17410094606168597	4.156589671312203E-4
80-81	1.367299233984277E-4	0.002543176575210755	0.0	0.17574717433940307	4.430049518109058E-4
82-83	1.421991203343648E-4	0.0025595841660185667	0.0	0.17635425519929204	4.976969211702769E-4
84-85	1.421991203343648E-4	0.0025978685445701265	0.0	0.17686289051433424	5.03166118106214E-4
86-87	1.421991203343648E-4	0.0026470913169935606	0.0	0.17714181955806702	5.03166118106214E-4
88-89	1.421991203343648E-4	0.0028658591944310447	0.0	0.17727854948146543	5.195737089140253E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13875	0.0	45.564903	1
CTTATAC	9315	0.0	37.286568	1
GTATCAA	25255	0.0	34.561836	1
TATACAC	12120	0.0	31.556608	3
ATCAACG	30740	0.0	27.748951	3
TCAACGC	30885	0.0	27.61822	4
CAACGCA	30960	0.0	27.551468	5
TATCAAC	31705	0.0	27.1394	2
AACGCAG	32000	0.0	26.75941	6
TTATACA	14215	0.0	25.451721	2
ACGCAGA	36660	0.0	23.312675	7
TACACAT	16430	0.0	23.051773	5
CGCAGAG	37155	0.0	22.979832	8
ACACATC	15570	0.0	22.598904	6
GTACATG	34555	0.0	22.316387	1
TACATGG	33920	0.0	22.307516	2
ACATCTC	16675	0.0	21.398779	8
ACATGGG	34610	0.0	21.39662	3
GCAGAGT	42375	0.0	20.090498	9
CACATCT	17710	0.0	19.681269	7
>>END_MODULE