FastQCFastQC Report
Fri 27 May 2016
SRR2139985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12358789
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121950.042.1095661
CTTATAC131850.038.572661
GTATCAA207450.034.608221
TATACAC183500.030.5452613
TCAACGC243200.028.690144
ATCAACG243250.028.5996443
CAACGCA246000.028.2461995
TTATACA191600.027.554862
AACGCAG257100.027.1228796
TATCAAC262650.026.9267792
ACGCAGA301550.023.1519787
CGCAGAG306150.022.8041118
ACATCTC239250.021.6701898
ACACATC237450.021.6610726
TACACAT255750.021.658145
GTACATG303500.020.029861
CACATCT261500.019.7002777
TACATGG300600.019.5375652
GCAGAGT372700.018.8096499
ACATGGG300500.018.6935773
Produced by FastQC (version 0.11.5)