##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12358789
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45267703817906	33.0	31.0	34.0	31.0	34.0
2	32.65702691420656	34.0	31.0	34.0	31.0	34.0
3	32.7494890478347	34.0	31.0	34.0	31.0	34.0
4	36.17737263739999	37.0	37.0	37.0	35.0	37.0
5	36.018156552393606	37.0	35.0	37.0	35.0	37.0
6	36.042745612049856	37.0	35.0	37.0	35.0	37.0
7	36.0573618499353	37.0	35.0	37.0	35.0	37.0
8	36.047367747762344	37.0	35.0	37.0	35.0	37.0
9	37.90672500355819	39.0	38.0	39.0	35.0	39.0
10-11	37.78645314682531	39.0	37.5	39.0	35.0	39.0
12-13	37.81465805428024	39.0	38.0	39.0	35.0	39.0
14-15	39.12786196123261	40.0	39.0	41.0	36.0	41.0
16-17	39.088311079669694	40.0	38.0	41.0	36.0	41.0
18-19	39.12102884837665	40.0	39.0	41.0	36.0	41.0
20-21	39.159551046511126	40.0	39.0	41.0	36.0	41.0
22-23	39.11894370839832	40.0	39.0	41.0	36.0	41.0
24-25	39.1105437146401	40.0	39.0	41.0	36.0	41.0
26-27	39.006095192238675	40.0	39.0	41.0	36.0	41.0
28-29	38.921079748519965	40.0	38.0	41.0	35.0	41.0
30-31	38.810077627599455	40.0	38.0	41.0	35.0	41.0
32-33	38.74876442604476	40.0	38.0	41.0	35.0	41.0
34-35	38.69294410329424	40.0	38.0	41.0	35.0	41.0
36-37	38.634792289550674	40.0	38.0	41.0	35.0	41.0
38-39	38.5456734975467	40.0	38.0	41.0	34.5	41.0
40-41	38.54784043284215	40.0	38.0	41.0	35.0	41.0
42-43	38.506596081191944	40.0	38.0	41.0	34.0	41.0
44-45	38.476973785174934	40.0	38.0	41.0	34.0	41.0
46-47	38.385414288653465	40.0	38.0	41.0	34.0	41.0
48-49	38.37332186901544	40.0	38.0	41.0	34.0	41.0
50-51	38.29101348243522	40.0	38.0	41.0	34.0	41.0
52-53	38.13150750540862	40.0	37.0	41.0	33.5	41.0
54-55	37.98194908862992	40.0	37.0	41.0	33.0	41.0
56-57	37.813559630283	39.5	37.0	41.0	33.0	41.0
58-59	37.613207645392734	39.0	36.0	41.0	33.0	41.0
60-61	37.45855832203877	39.0	36.0	41.0	32.5	41.0
62-63	37.30408974069907	39.0	35.5	40.5	32.0	41.0
64-65	37.060996047977824	39.0	35.0	40.0	32.0	41.0
66-67	36.876419964941846	38.0	35.0	40.0	32.0	41.0
68-69	36.607886418613525	37.5	35.0	40.0	32.0	41.0
70-71	36.28806520070952	37.0	35.0	39.5	31.0	41.0
72-73	36.02926424799382	36.5	35.0	39.0	31.0	41.0
74-75	35.66843245972508	36.0	34.5	39.0	31.0	40.0
76-77	33.63966826187402	34.5	32.0	36.5	28.0	39.0
78-79	34.80648600743511	35.0	34.0	37.0	30.5	39.0
80-81	34.77727245151203	35.0	34.0	37.0	31.0	39.0
82-83	34.52921075971104	35.0	34.0	36.5	31.0	39.0
84-85	34.25309446931197	35.0	34.0	36.0	31.0	37.0
86-87	34.02242248347325	35.0	34.0	36.0	30.0	37.0
88-89	33.81205020731027	35.0	34.0	35.0	30.0	36.5
90-91	33.75018029376852	35.0	34.0	35.0	30.0	36.0
92-93	33.66153804906685	35.0	34.0	35.0	30.0	36.0
94-95	33.572240310618476	35.0	34.0	35.0	30.0	36.0
96-97	33.571143421915	35.0	34.0	35.0	30.0	35.5
98-99	33.62835997810912	35.0	34.0	35.0	30.5	35.0
100-101	32.392562543321404	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	23.0
20	62.0
21	256.0
22	1017.0
23	3224.0
24	8362.0
25	18152.0
26	33012.0
27	54281.0
28	80761.0
29	113477.0
30	153600.0
31	202516.0
32	270761.0
33	370990.0
34	548850.0
35	858684.0
36	1562270.0
37	2946162.0
38	3558182.0
39	1543568.0
40	30577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81038198807342	20.71600219082954	14.087035550165957	24.386580270931077
2	14.409167149934826	21.065588288864976	42.00659089572145	22.518653665478748
3	18.176896278144124	26.09731694459313	30.647114108242228	25.078672669020513
4	12.421185178136628	18.18613681227079	38.61196422023129	30.780713789361293
5	13.008888897519725	37.803140566858175	35.98089877421637	13.20707176140573
6	29.814727037663552	38.18597821233851	18.6832506684744	13.316044081523534
7	26.09231373721163	32.56976067800817	23.12590659165716	18.21201899312303
8	23.911761869128874	37.54678453802575	21.466755952689194	17.074697640156185
9	24.905789351844174	16.9086559868841	21.23411276278061	36.95144189849112
10-11	23.895572003775634	26.91785186592799	29.030834229428276	20.155741900868104
12-13	24.60796883186238	24.225305501556587	29.143005678151756	22.023719988429274
14-15	21.837736773920216	25.892978297296747	26.3513931752018	25.91789175358124
16-17	21.761717857413345	29.215258443738296	27.044495783713945	21.978527915134418
18-19	21.759886830335883	28.23164955725031	28.47545985290306	21.53300375951074
20-21	22.3452235783048	27.437509659492278	28.4921603358388	21.72510642636412
22-23	21.938509695259476	27.738877419970915	28.338590782442765	21.984022102326847
24-25	21.913774325138522	27.82456003598123	28.504639365955953	21.7570262729243
26-27	22.030191216979052	28.010160774333926	28.32186690057717	21.637781108109856
28-29	21.498206832985165	27.79753888126038	28.57700565520926	22.127248630545196
30-31	22.13576066412542	27.514488060654536	28.68375650861329	21.665994766606758
32-33	21.745984166232454	28.065421573948974	28.383217282790916	21.805376977027656
34-35	21.691039694267182	28.126450694371798	28.80478312323465	21.377726488126374
36-37	22.08565188439619	27.838892226687506	28.333176784141017	21.742279104775292
38-39	21.78924703283208	27.723715422943933	28.55878722929403	21.928250314929958
40-41	21.887078588619442	27.598301076676314	28.64878539252934	21.865834942174903
42-43	21.951904901281853	27.561534886427953	28.631812994339324	21.85474721795087
44-45	21.725685876085294	27.93811445802824	28.429265358928273	21.90693430695819
46-47	22.224113680484884	27.55840264351395	27.896596530991747	22.320887145009415
48-49	21.999286757018925	27.633875759915362	28.145583086610493	22.22125439645522
50-51	21.857902898405573	27.907872589386884	28.139128658418617	22.095095853788923
52-53	21.938891956052554	28.02342379976318	28.232332523753833	21.80535172043043
54-55	21.849002144450782	27.99057921832242	27.967203260720446	22.193215376506352
56-57	22.239364689139972	28.149052576125904	27.601213112295202	22.01036962243892
58-59	21.8172899226526	28.39863365955633	28.065859579345627	21.71821683844544
60-61	22.183220705774705	28.58353494611645	27.52396614052793	21.70927820758092
62-63	21.963810388787135	28.71207055132362	27.527021931828838	21.79709712806041
64-65	21.941169065665324	28.487532144608142	27.621716513608163	21.949582276118374
66-67	22.07318759407923	28.302618487948894	27.554975181134374	22.069218736837502
68-69	22.03367623101477	28.224882522225688	27.676446912326057	22.06499433443349
70-71	22.046600080976315	27.95888658475873	27.74676077056229	22.247752563702665
72-73	22.086864284785307	28.009777668995973	27.732844716037263	22.17051333018146
74-75	21.963571005356965	28.140304431405283	27.744893060988137	22.15123150224962
76-77	22.063781784549743	28.118393301279088	27.609954973567035	22.207869940604134
78-79	22.040730950125443	28.14529714556847	27.571635356730646	22.242336547575448
80-81	22.019814157817347	28.15877854785108	27.641380450652616	22.180026843678956
82-83	22.10712514790924	28.070212693759732	27.757958639048912	22.064703519282112
84-85	22.211147122293255	28.06402272066768	27.642066036989437	22.08276412004962
86-87	21.999850902252362	28.124842638580027	27.697708536408683	22.177597922758927
88-89	22.08901735477179	28.083499067722645	27.63224736288739	22.195236214618173
90-91	22.11127213888574	28.069040036357134	27.71846332397449	22.10122450078264
92-93	22.137979635974826	28.07152582496904	27.66710714717971	22.123387391876427
94-95	21.990712095484586	28.148554860774453	27.71681889450387	22.143914149237094
96-97	22.013260381190946	28.134566425732878	27.768842207269362	22.083330985806814
98-99	22.19670516565608	28.784592999969043	27.796851208411244	21.221850625963633
100-101	26.225493980244668	35.79508071048643	28.388033525125888	9.59139178414301
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2693.0
1	2341.0
2	2959.0
3	4245.5
4	5721.0
5	8898.0
6	11629.5
7	13751.5
8	15392.0
9	15374.0
10	14084.0
11	12687.5
12	13490.0
13	17446.5
14	26465.0
15	38384.5
16	47811.0
17	53192.0
18	54882.5
19	54172.0
20	52709.0
21	51883.0
22	54094.0
23	62241.0
24	76914.0
25	97116.0
26	122381.0
27	149893.5
28	178285.5
29	207516.0
30	232812.0
31	256998.0
32	283657.5
33	309590.0
34	335029.0
35	359455.5
36	386809.0
37	412391.5
38	431753.5
39	448220.5
40	465797.5
41	484891.5
42	500548.0
43	514239.0
44	526639.5
45	542324.0
46	562551.5
47	583356.5
48	584780.5
49	573574.5
50	564961.5
51	543498.5
52	508085.5
53	467408.0
54	428256.0
55	390928.5
56	353764.5
57	314802.5
58	276523.0
59	238426.5
60	198495.0
61	161214.0
62	128195.5
63	99791.5
64	75369.0
65	55995.5
66	42106.5
67	32026.0
68	24333.0
69	17779.5
70	12770.5
71	9142.5
72	6387.0
73	4420.0
74	3036.5
75	2109.0
76	1490.5
77	992.0
78	664.0
79	458.0
80	324.5
81	216.5
82	148.5
83	101.5
84	76.5
85	64.5
86	48.0
87	36.0
88	28.5
89	21.0
90	13.0
91	9.0
92	7.5
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018286581314722664
3	0.00245978792906004
4	5.906727592808647E-4
5	8.091407661381709E-6
6	4.126617907304672E-4
7	0.0
8	4.045703830690855E-5
9	3.883875677463221E-4
10-11	5.86627055450174E-4
12-13	1.1327970725934393E-4
14-15	4.854844596829026E-5
16-17	3.641133447621769E-4
18-19	0.0
20-21	8.496300124276595E-5
22-23	4.046264993940718E-5
24-25	0.0
26-27	1.0526771381320107E-4
28-29	2.3500681823661015E-4
30-31	6.696633492761913E-4
32-33	3.3789750078795254E-4
34-35	0.0010175189382136756
36-37	1.2737582100393594E-4
38-39	1.6982909849288274E-4
40-41	2.6329848556980114E-4
42-43	0.0013284830347444704
44-45	3.196310486020744E-4
46-47	1.6820388484555023E-4
48-49	4.7566761586357605E-4
50-51	0.0
52-53	4.729750743474428E-4
54-55	5.144489299778146E-4
56-57	7.133751810189521E-4
58-59	5.982190323802514E-4
60-61	7.101585642982972E-4
62-63	7.630546489247559E-5
64-65	7.547158857534314E-4
66-67	0.002396975135263321
68-69	0.001166192033154142
70-71	2.6776473785958466E-4
72-73	0.0
74-75	4.6649459581560566E-5
76-77	5.250834462612798E-6
78-79	9.418247114158626E-4
80-81	1.5109799676434432E-4
82-83	8.75687606327631E-5
84-85	7.767613424078292E-4
86-87	2.5318421345792245E-4
88-89	3.426229453901282E-5
90-91	1.1602352121640917E-5
92-93	1.297610968445994E-4
94-95	6.001297960722945E-4
96-97	6.123981849742594E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	288.0
20-21	1007.0
22-23	2173.0
24-25	4396.0
26-27	7665.0
28-29	15078.0
30-31	36653.0
32-33	42883.0
34-35	58786.0
36-37	91388.0
38-39	108770.0
40-41	106409.0
42-43	117507.0
44-45	137580.0
46-47	137381.0
48-49	132098.0
50-51	123239.0
52-53	121732.0
54-55	141169.0
56-57	152819.0
58-59	150014.0
60-61	150450.0
62-63	148278.0
64-65	149585.0
66-67	153857.0
68-69	137499.0
70-71	130638.0
72-73	122713.0
74-75	124348.0
76-77	123382.0
78-79	130506.0
80-81	131285.0
82-83	124824.0
84-85	123823.0
86-87	129063.0
88-89	135623.0
90-91	141308.0
92-93	143726.0
94-95	156975.0
96-97	235474.0
98-99	967775.0
100-101	7008622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98295161591537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.87230626608934	76.37613855021799
2	6.961486828973627	11.83215396722996
3	1.7537029979555245	4.471045710718203
4	0.6465538663159442	2.197842237528436
5	0.30757775430479795	1.3069432706108275
6	0.15522331943353382	0.7914801507049068
7	0.08836475385270068	0.5256648320851434
8	0.05592494410388458	0.3802133455122556
9	0.03825137322172328	0.2925643139769627
>10	0.117064571919442	1.5383913809607646
>50	0.00272443099282104	0.15709240228251195
>100	7.559709275573663E-4	0.09852083622474732
>500	6.292190910398933E-5	0.03194900194738469
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.09140766138171E-6	0.0	8.09140766138171E-6	0.0
3	0.0	8.09140766138171E-6	0.0	1.618281532276342E-5	0.0
4	0.0	7.282266895243539E-5	0.0	1.618281532276342E-5	0.0
5	0.0	8.90054842751988E-5	0.0	2.427422298414513E-5	0.0
6	0.0	9.709689193658052E-5	0.0	2.427422298414513E-5	0.0
7	0.0	9.709689193658052E-5	0.0	3.236563064552684E-5	0.0
8	0.0	1.0518829959796223E-4	0.0	3.236563064552684E-5	0.0
9	0.0	1.699195608890159E-4	0.0	4.854844596829026E-5	8.09140766138171E-6
10-11	0.0	1.699195608890159E-4	0.0	1.1327970725934393E-4	8.09140766138171E-6
12-13	0.0	1.7396526471970676E-4	0.0	1.3350822641279821E-4	8.09140766138171E-6
14-15	0.0	2.0228519153454275E-4	0.0	1.4969104173556162E-4	2.0228519153454275E-5
16-17	0.0	2.0228519153454275E-4	0.0	1.618281532276342E-4	4.045703830690855E-5
18-19	0.0	2.1846800685730616E-4	0.0	1.9823948770385187E-4	4.854844596829026E-5
20-21	0.0	2.3869652601076044E-4	0.0	2.5487934133352385E-4	4.854844596829026E-5
22-23	0.0	2.710621566562873E-4	0.0	2.8319926814835984E-4	7.282266895243539E-5
24-25	4.045703830690855E-6	3.034277873018141E-4	0.0	2.993820834711233E-4	8.90054842751988E-5
26-27	8.09140766138171E-6	3.155648987938867E-4	0.0	3.8029616008494033E-4	9.709689193658052E-5
28-29	8.09140766138171E-6	3.1961060262457753E-4	0.0	7.889122469847168E-4	9.709689193658052E-5
30-31	8.09140766138171E-6	3.560219371007952E-4	0.0	0.0022655941451868787	9.709689193658052E-5
32-33	8.09140766138171E-6	3.560219371007952E-4	0.0	0.005053084084532878	9.709689193658052E-5
34-35	8.09140766138171E-6	4.207531983918489E-4	0.0	0.00861330345554083	9.709689193658052E-5
36-37	8.09140766138171E-6	4.895301635135935E-4	0.0	0.013787758654994434	9.709689193658052E-5
38-39	8.09140766138171E-6	5.01667275005666E-4	0.0	0.02195603468915927	9.709689193658052E-5
40-41	8.09140766138171E-6	5.58307128635338E-4	0.0	0.03037919006465763	9.709689193658052E-5
42-43	8.09140766138171E-6	5.866270554501739E-4	0.0	0.038486780541362106	1.0114259576727137E-4
44-45	8.09140766138171E-6	6.351755014184642E-4	0.0	0.04762602549489274	1.1327970725934394E-4
46-47	1.2137111492072565E-5	6.554040205719185E-4	0.0	0.057392354542180474	1.173254110900348E-4
48-49	1.618281532276342E-5	6.594497244026094E-4	0.0	0.06699280973240987	1.2946252258210736E-4
50-51	1.618281532276342E-5	7.322723933550447E-4	0.0	0.07642334536175024	1.2946252258210736E-4
52-53	1.618281532276342E-5	7.403638010164264E-4	0.0	0.08550190475782053	1.3350822641279821E-4
54-55	1.618281532276342E-5	7.646380240005716E-4	0.0	0.0946249668960284	1.3755393024348907E-4
56-57	1.618281532276342E-5	7.848665431540258E-4	0.0	0.10284988278382291	1.3755393024348907E-4
58-59	1.618281532276342E-5	7.929579508154076E-4	0.0	0.11142272920105684	1.4159963407417992E-4
60-61	1.618281532276342E-5	7.970036546460984E-4	0.0	0.11957482241989892	1.5373674556625247E-4
62-63	1.618281532276342E-5	8.131864699688618E-4	0.0	0.1278523324574924	1.618281532276342E-4
64-65	1.618281532276342E-5	8.90054842751988E-4	0.0	0.13596396863802757	1.6587385705832506E-4
66-67	1.618281532276342E-5	9.062376580747514E-4	0.0	0.14390568525767372	1.699195608890159E-4
68-69	1.618281532276342E-5	9.345575848895874E-4	0.0	0.15150756275554184	1.699195608890159E-4
70-71	1.618281532276342E-5	9.547861040430418E-4	0.0	0.15867250423969534	1.699195608890159E-4
72-73	2.0228519153454275E-5	9.628775117044235E-4	0.0	0.1643445810103239	1.9419378387316104E-4
74-75	2.427422298414513E-5	9.952431423499502E-4	0.0	0.16909828301138566	1.9419378387316104E-4
76-77	2.427422298414513E-5	0.0010680658113023855	0.0	0.17300643291183304	1.9419378387316104E-4
78-79	2.427422298414513E-5	0.001084248626625149	0.0	0.17698740548123282	1.9419378387316104E-4
80-81	2.427422298414513E-5	0.0010882943304558398	0.0	0.17867850968246163	1.982394877038519E-4
82-83	2.427422298414513E-5	0.0010923400342865309	0.0	0.17935818792601768	2.0228519153454275E-4
84-85	2.427422298414513E-5	0.0011085228496092942	0.0	0.18005404898489652	2.0228519153454275E-4
86-87	2.427422298414513E-5	0.0011327970725934393	0.0	0.180361522476029	2.0228519153454275E-4
88-89	2.427422298414513E-5	0.0011489798879162029	0.0	0.1805354877407487	2.063308953652336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12195	0.0	42.109566	1
CTTATAC	13185	0.0	38.57266	1
GTATCAA	20745	0.0	34.60822	1
TATACAC	18350	0.0	30.545261	3
TCAACGC	24320	0.0	28.69014	4
ATCAACG	24325	0.0	28.599644	3
CAACGCA	24600	0.0	28.246199	5
TTATACA	19160	0.0	27.55486	2
AACGCAG	25710	0.0	27.122879	6
TATCAAC	26265	0.0	26.926779	2
ACGCAGA	30155	0.0	23.151978	7
CGCAGAG	30615	0.0	22.804111	8
ACATCTC	23925	0.0	21.670189	8
ACACATC	23745	0.0	21.661072	6
TACACAT	25575	0.0	21.65814	5
GTACATG	30350	0.0	20.02986	1
CACATCT	26150	0.0	19.700277	7
TACATGG	30060	0.0	19.537565	2
GCAGAGT	37270	0.0	18.809649	9
ACATGGG	30050	0.0	18.693577	3
>>END_MODULE