FastQCFastQC Report
Fri 27 May 2016
SRR2139984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12380333
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130050.043.178431
GTATCAA215650.033.762371
ATCAACG255200.027.5371133
TCAACGC256300.027.4674324
CAACGCA261150.026.9891245
TATCAAC273550.026.2517812
AACGCAG272850.025.9687046
ACGCAGA317900.022.1970147
CGCAGAG323950.021.7311888
GTACATG356050.021.545491
TACATGG350000.021.3601822
ACATGGG355200.020.3458123
CATGGGG261150.018.5122814
GCAGAGT393400.017.852589
GAGTACT207100.016.8355412-13
AGAGTAC348300.016.77756110-11
CAGAGTA380650.016.3882410-11
TATACCG10700.016.3028135
GTGGTAT71700.016.1616441
TGGTATC66900.015.334562
Produced by FastQC (version 0.11.5)