##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12380333
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25010837753718	33.0	31.0	34.0	30.0	34.0
2	32.46139736305962	34.0	31.0	34.0	31.0	34.0
3	32.55139009588837	34.0	31.0	34.0	31.0	34.0
4	36.052179614231704	37.0	35.0	37.0	35.0	37.0
5	35.84705419474581	37.0	35.0	37.0	35.0	37.0
6	35.86499280754403	37.0	35.0	37.0	35.0	37.0
7	35.88075506531206	37.0	35.0	37.0	35.0	37.0
8	35.862811767664084	37.0	35.0	37.0	35.0	37.0
9	37.689079041734985	39.0	37.0	39.0	35.0	39.0
10-11	37.54473264168257	39.0	37.0	39.0	35.0	39.0
12-13	37.574206404625784	39.0	37.0	39.0	35.0	39.0
14-15	38.822166374684755	40.0	38.0	41.0	35.5	41.0
16-17	38.775735717286445	40.0	38.0	41.0	35.0	41.0
18-19	38.80401427812968	40.0	38.0	41.0	35.0	41.0
20-21	38.84103112384078	40.0	38.0	41.0	35.0	41.0
22-23	38.79881291961531	40.0	38.0	41.0	35.0	41.0
24-25	38.78077907347139	40.0	38.0	41.0	35.0	41.0
26-27	38.67061952333802	40.0	38.0	41.0	35.0	41.0
28-29	38.587617982020745	40.0	38.0	41.0	34.5	41.0
30-31	38.46247625368545	40.0	38.0	41.0	34.0	41.0
32-33	38.398707483378956	40.0	38.0	41.0	34.0	41.0
34-35	38.33405631606515	40.0	38.0	41.0	34.0	41.0
36-37	38.26665743732303	40.0	38.0	41.0	34.0	41.0
38-39	38.184694408347326	40.0	38.0	41.0	34.0	41.0
40-41	38.1628269497936	40.0	38.0	41.0	34.0	41.0
42-43	38.10130274379209	40.0	38.0	41.0	33.0	41.0
44-45	38.05985204677561	40.0	37.5	41.0	33.0	41.0
46-47	37.95580169897747	40.0	37.0	41.0	33.0	41.0
48-49	37.935858757115554	40.0	37.0	41.0	33.0	41.0
50-51	37.86228580422215	40.0	37.0	41.0	33.0	41.0
52-53	37.678147857309234	39.5	37.0	41.0	33.0	41.0
54-55	37.522781248508394	39.0	36.0	40.5	32.5	41.0
56-57	37.33821274725325	39.0	36.0	40.0	32.0	41.0
58-59	37.11454783026742	39.0	35.5	40.0	31.5	41.0
60-61	36.94795191367309	39.0	35.0	40.0	31.0	41.0
62-63	36.77726877027686	38.0	35.0	40.0	31.0	41.0
64-65	36.50592882196712	38.0	35.0	40.0	31.0	41.0
66-67	36.30607385113825	37.5	35.0	40.0	31.0	41.0
68-69	36.022828732944006	37.0	34.5	39.5	31.0	41.0
70-71	35.70131337599676	36.5	34.0	39.0	30.5	41.0
72-73	35.448594611805724	36.0	34.0	39.0	30.0	40.0
74-75	35.093286379750694	35.5	34.0	38.5	30.0	40.0
76-77	33.041760690387484	34.0	31.0	36.0	27.5	38.5
78-79	34.262152879245264	35.0	33.5	37.0	29.5	39.0
80-81	34.271586173324025	35.0	34.0	36.5	30.0	39.0
82-83	34.043773467161635	35.0	34.0	36.0	30.0	37.5
84-85	33.78214551385284	35.0	33.0	36.0	29.0	37.0
86-87	33.561513475278105	35.0	33.0	35.0	29.0	37.0
88-89	33.36277183934388	35.0	33.0	35.0	29.0	36.0
90-91	33.322127152379025	35.0	33.0	35.0	29.0	36.0
92-93	33.245466330661415	35.0	33.0	35.0	29.0	36.0
94-95	33.16830797162988	35.0	33.0	35.0	29.0	35.5
96-97	33.19160525546751	35.0	33.0	35.0	29.0	35.0
98-99	33.28475392355556	34.0	33.0	35.0	29.5	35.0
100-101	32.047380985601954	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	28.0
20	99.0
21	380.0
22	1422.0
23	4444.0
24	11142.0
25	23539.0
26	42514.0
27	69048.0
28	102018.0
29	143591.0
30	192934.0
31	254809.0
32	340625.0
33	471343.0
34	695074.0
35	1082896.0
36	1874381.0
37	3029032.0
38	2969206.0
39	1057756.0
40	14049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49936031607551	20.486169475409103	13.871097005225948	24.143373203289443
2	14.925998835393099	21.22941552452181	41.404085558624	22.440500081461096
3	18.6968371727396	25.960527380739094	30.107593412215678	25.23504203430563
4	12.944720533267636	17.85323831659636	38.477780178550155	30.72426097158585
5	13.372332290631	37.76048475602147	35.52444599421453	13.342736959133001
6	30.284937981577624	37.79766407412825	18.6196217159347	13.297776228359423
7	26.703377041635314	32.259899632748166	23.189004689938468	17.847718635678056
8	24.215792055879625	37.26670040837374	21.203351188005872	17.314156347740763
9	25.56359360357265	16.761575621112467	21.2749608530853	36.399869922229584
10-11	24.478245447060303	26.74758600791477	28.720398314398704	20.05377023062622
12-13	25.051404164028067	24.13057578479393	28.939470062163174	21.87854998901482
14-15	22.184960159788943	25.523917736932884	26.392174005686925	25.89894809759125
16-17	21.971326822064864	28.64324727362812	27.12315657455594	22.262269329751078
18-19	22.031261194670613	28.04095818747363	28.541187058538732	21.386593559317024
20-21	22.742893727947102	27.27702387863148	28.336164771656748	21.643917621764672
22-23	22.474279406527884	27.21124962111444	28.193334716386794	22.12113625597088
24-25	22.272175342239052	27.281040481218888	28.338546128520292	22.108238048021768
26-27	22.27081679203594	27.462930550863955	28.19521478851506	22.071037868585037
28-29	22.11887852269792	27.60387816228444	28.202896688831625	22.074346626186017
30-31	22.49396481574178	27.431968072725667	28.108362475283442	21.965704636249107
32-33	22.298458045058275	27.568992995251335	28.0390279661685	22.093520993521885
34-35	22.33064962063326	27.565406575418315	28.120126996929486	21.983816807018943
36-37	22.326149686382074	27.51251880362683	28.080584324548795	22.080747185442302
38-39	22.192062078955548	27.56138856445556	28.1681797912053	22.07836956538359
40-41	22.42543687667435	27.371571268596327	28.079380975102953	22.12361087962637
42-43	22.2992377069563	27.469662560657216	27.94135115045546	22.289748581931022
44-45	22.33954078572393	27.658612824787664	27.795334885547014	22.206511503941393
46-47	22.473764675825837	27.520886660527456	27.521587490249654	22.483761173397056
48-49	22.42766177815912	27.602712613455978	27.561853870013266	22.407771738371647
50-51	22.340430444300306	27.688759651587013	27.54872635090994	22.422083553202736
52-53	22.50079624173899	27.62681571553695	27.439276792112654	22.4331112506114
54-55	22.496094958819484	27.527204177302966	27.429454670001796	22.547246193875754
56-57	22.56031340874517	27.64798300749562	27.348015641372854	22.443687942386354
58-59	22.434689788413408	27.756255776856364	27.423908620729666	22.385145814000563
60-61	22.562144612166644	27.74066815365565	27.351473966555908	22.345713267621793
62-63	22.58297276626005	27.7692487399558	27.236896589391606	22.410881904392543
64-65	22.462704137488814	27.726841946127344	27.303002882276967	22.50745103410687
66-67	22.552941225220366	27.71585683543634	27.17657431189248	22.554627627450813
68-69	22.50533181195712	27.684612463897412	27.274416770537357	22.53563895360811
70-71	22.50727652269348	27.453196046515366	27.31808940451781	22.721438026273344
72-73	22.572884220190065	27.496831164399133	27.29290715557025	22.637377459840554
74-75	22.433816664922812	27.65006071047067	27.272931889908435	22.64319073469808
76-77	22.53990396401924	27.63267071672723	27.154744744783287	22.67268057447024
78-79	22.531784743479157	27.661957151219475	27.09880204718426	22.707456058117106
80-81	22.496352482507973	27.694922380416497	27.151755867545514	22.656969269530016
82-83	22.584990992683633	27.592837540713106	27.30183275750277	22.520338709100493
84-85	22.723601977829365	27.604009207323582	27.136407463633837	22.535981351213216
86-87	22.45785436338377	27.63968276772203	27.253595771438082	22.648867097456122
88-89	22.560525211681416	27.56927875830008	27.207951945670494	22.662244084348007
90-91	22.562186766569248	27.604826667559866	27.23584817813754	22.59713838773335
92-93	22.604224956013823	27.57394514754553	27.224736587401416	22.597093309039227
94-95	22.420273047238158	27.714628778793948	27.249640395870173	22.615457778097724
96-97	22.434094513047853	27.72412547161091	27.28620405282282	22.55557596251841
98-99	22.66944735014305	28.304028283685952	27.326698841987223	21.699825524183776
100-101	26.89259829429482	35.3512597437173	27.86788163819741	9.888260323790465
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2456.0
1	2216.5
2	2927.5
3	4164.5
4	5682.0
5	9248.5
6	12259.5
7	14233.5
8	15452.0
9	15052.5
10	13812.0
11	12499.5
12	13103.5
13	16949.0
14	26015.5
15	38037.0
16	47634.5
17	53123.0
18	54343.0
19	52444.5
20	49871.5
21	48005.0
22	48995.5
23	55224.5
24	67525.0
25	85833.0
26	109226.5
27	134336.0
28	159594.0
29	185494.5
30	208405.0
31	230964.0
32	256091.0
33	280879.5
34	307461.0
35	335518.5
36	366401.5
37	394358.0
38	414818.0
39	434900.0
40	457554.0
41	478398.0
42	495053.5
43	512050.0
44	528995.5
45	544362.5
46	555551.5
47	562683.0
48	563907.0
49	557970.0
50	550438.0
51	536791.0
52	511940.5
53	482699.5
54	451720.0
55	417723.5
56	383528.5
57	345923.0
58	306835.5
59	267672.0
60	224809.5
61	183403.0
62	147060.5
63	115191.0
64	86987.0
65	64686.5
66	48441.0
67	36848.0
68	28221.5
69	20582.0
70	14773.5
71	10526.0
72	7212.0
73	4921.5
74	3437.5
75	2308.0
76	1537.0
77	1082.5
78	759.0
79	536.0
80	382.0
81	263.0
82	192.5
83	136.0
84	93.0
85	64.5
86	42.5
87	30.5
88	22.0
89	13.5
90	8.0
91	5.0
92	3.5
93	2.0
94	1.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001534692160542047
3	0.0016720067222747562
4	4.684849753233617E-4
5	1.6154654321495228E-5
6	4.846396296448569E-4
7	0.0
8	5.6541290125233306E-5
9	3.796343765551379E-4
10-11	5.048329475467259E-4
12-13	1.0500525308971898E-4
14-15	1.6154654321495228E-5
16-17	4.2002101235887594E-4
18-19	0.0
20-21	9.693239268523932E-5
22-23	1.2117873463468793E-5
24-25	0.0
26-27	8.892528897384218E-5
28-29	2.1438690663923483E-4
30-31	6.603866997632493E-4
32-33	3.494641373806915E-4
34-35	9.217249928260431E-4
36-37	1.6400868721214425E-4
38-39	1.7356343712633497E-4
40-41	2.1677763875290493E-4
42-43	0.0013038964548064862
44-45	3.612026228579728E-4
46-47	1.7628201200721276E-4
48-49	3.479548756459293E-4
50-51	0.0
52-53	4.7543226012276374E-4
54-55	7.229010981945297E-4
56-57	6.539448359384105E-4
58-59	5.605946015566977E-4
60-61	7.02150729534608E-4
62-63	6.58612525455374E-5
64-65	7.138892338031501E-4
66-67	0.0022211833144516694
68-69	0.0012200793832449915
70-71	2.2741012838241052E-4
72-73	0.0
74-75	1.2166412185878446E-4
76-77	0.0
78-79	8.113361973847826E-4
80-81	1.370898410364203E-4
82-83	1.069338644524513E-4
84-85	8.075583559824546E-4
86-87	2.7479817173478767E-4
88-89	2.789263033194127E-5
90-91	2.833761250173851E-5
92-93	1.1530539614264246E-4
94-95	5.284598220511369E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	365.0
20-21	1090.0
22-23	2458.0
24-25	4938.0
26-27	7602.0
28-29	14508.0
30-31	35347.0
32-33	41787.0
34-35	57195.0
36-37	88927.0
38-39	106599.0
40-41	104761.0
42-43	115858.0
44-45	135247.0
46-47	135053.0
48-49	129282.0
50-51	117992.0
52-53	115199.0
54-55	123944.0
56-57	128817.0
58-59	128586.0
60-61	126131.0
62-63	121987.0
64-65	126048.0
66-67	132625.0
68-69	131487.0
70-71	129263.0
72-73	123266.0
74-75	125301.0
76-77	122810.0
78-79	129602.0
80-81	132603.0
82-83	127102.0
84-85	126209.0
86-87	132913.0
88-89	138930.0
90-91	148228.0
92-93	153923.0
94-95	173189.0
96-97	261135.0
98-99	1022829.0
100-101	7099197.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.62115395141579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.68205266735288	75.88998785443047
2	6.982214171682759	11.816860406874477
3	1.8293608048320302	4.644078668951314
4	0.7049793133159056	2.3862465201867455
5	0.3198125161985692	1.353145208441439
6	0.1671229967397501	0.8485284501561811
7	0.09828714152619951	0.5822019934173185
8	0.05858598322349666	0.39660908046004584
9	0.039900658208573844	0.30387957669274945
>10	0.11478996862224733	1.4755822352878103
>50	0.0022086106503821285	0.13130313001809082
>100	5.910196248732374E-4	0.08429982087596376
>500	6.27659528564713E-5	0.0413619560011568
>1k	3.1382069590537955E-5	0.04591509820628467
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.077327160747614E-6	8.077327160747614E-6
2	0.0	0.0	0.0	8.077327160747614E-6	8.077327160747614E-6
3	0.0	0.0	0.0	2.4231981482242846E-5	8.077327160747614E-6
4	0.0	8.077327160747614E-5	0.0	4.038663580373807E-5	8.077327160747614E-6
5	0.0	8.885059876822377E-5	0.0	4.038663580373807E-5	8.077327160747614E-6
6	5.6541290125233306E-5	8.885059876822377E-5	0.0	4.038663580373807E-5	8.077327160747614E-6
7	5.6541290125233306E-5	8.885059876822377E-5	0.0	4.038663580373807E-5	1.6154654321495228E-5
8	6.461861728598091E-5	1.05005253089719E-4	0.0	4.038663580373807E-5	1.6154654321495228E-5
9	6.461861728598091E-5	2.0193317901869037E-4	0.0	6.461861728598091E-5	1.6154654321495228E-5
10-11	6.865728086635471E-5	2.0193317901869037E-4	0.0	8.481193518784996E-5	1.6154654321495228E-5
12-13	7.269594444672853E-5	2.0193317901869037E-4	0.0	1.3327589815233563E-4	1.6154654321495228E-5
14-15	7.269594444672853E-5	2.786677870457927E-4	0.0	1.5346921605420468E-4	1.6154654321495228E-5
16-17	1.3731456173270945E-4	2.9886110494766176E-4	0.0	1.6154654321495228E-4	1.6154654321495228E-5
18-19	1.3731456173270945E-4	3.190544228495308E-4	0.0	1.7770119753644754E-4	1.6154654321495228E-5
20-21	1.3731456173270945E-4	3.473250679121474E-4	0.0	1.8577852469719514E-4	1.6154654321495228E-5
22-23	1.3731456173270945E-4	4.1194368519812833E-4	0.0	1.8981718827756894E-4	1.6154654321495228E-5
24-25	1.4135322531308325E-4	4.442529938411188E-4	0.0	2.3828115124205465E-4	1.6154654321495228E-5
26-27	1.4539188889345705E-4	4.6848497532336165E-4	0.0	3.35209077171026E-4	1.6154654321495228E-5
28-29	1.5346921605420468E-4	4.6848497532336165E-4	0.0	8.60235342619621E-4	1.6154654321495228E-5
30-31	1.5346921605420468E-4	5.169489382878473E-4	0.0	0.0030734229846644677	1.6154654321495228E-5
32-33	1.5346921605420468E-4	5.25026265448595E-4	0.0	0.007346329052699956	1.6154654321495228E-5
34-35	1.5346921605420468E-4	6.623408271813044E-4	0.0	0.012519857099158803	1.6154654321495228E-5
36-37	1.5346921605420468E-4	7.996553889140139E-4	0.0	0.02067391886793352	1.6154654321495228E-5
38-39	1.5346921605420468E-4	8.279260339766304E-4	0.0	0.03242239122324092	2.4231981482242846E-5
40-41	1.5346921605420468E-4	8.885059876822376E-4	0.0	0.044449531365594125	2.4231981482242846E-5
42-43	1.5346921605420468E-4	9.248539599056018E-4	0.0	0.054804664785672565	2.4231981482242846E-5
44-45	1.5750787963457848E-4	0.0010379365401560685	0.0	0.06635524262554165	2.4231981482242846E-5
46-47	1.6154654321495228E-4	0.0011146711481831708	0.0	0.07817237226171542	2.4231981482242846E-5
48-49	1.6154654321495228E-4	0.0011267871389242924	0.0	0.08919792383613592	2.827064506261665E-5
50-51	1.6154654321495228E-4	0.0012277537284336375	0.0	0.09980345439819752	3.2309308642990457E-5
52-53	1.6154654321495228E-4	0.0012439083827551327	0.0	0.10946797634603206	3.2309308642990457E-5
54-55	1.6154654321495228E-4	0.0012479470463355065	0.0	0.11943539806239462	3.2309308642990457E-5
56-57	1.6154654321495228E-4	0.0012519857099158804	0.0	0.1291645386275151	4.4425299384111885E-5
58-59	1.6154654321495228E-4	0.0012519857099158804	0.0	0.1383080729734814	4.846396296448569E-5
60-61	1.8981718827756894E-4	0.0012762176913981233	0.0	0.1469911996712851	4.846396296448569E-5
62-63	2.10010506179438E-4	0.0012842950185588708	0.0	0.15507660415919344	4.846396296448569E-5
64-65	2.10010506179438E-4	0.0013973775988093374	0.0	0.1630085394310476	4.846396296448569E-5
66-67	2.10010506179438E-4	0.0014054549259700849	0.0	0.1711666398634027	4.846396296448569E-5
68-69	2.10010506179438E-4	0.0014216095802915803	0.0	0.17922377370624842	4.846396296448569E-5
70-71	2.180878333401856E-4	0.0014418028981934491	0.0	0.18639240156141196	4.846396296448569E-5
72-73	2.5847446914392365E-4	0.001445841561773823	0.0	0.191751708132568	4.846396296448569E-5
74-75	2.5847446914392365E-4	0.0014700735432560657	0.0	0.19606096217282687	4.846396296448569E-5
76-77	2.5847446914392365E-4	0.0014983441883186827	0.0	0.19971191404948477	4.846396296448569E-5
78-79	2.5847446914392365E-4	0.001510460179059804	0.0	0.20324170601873148	4.846396296448569E-5
80-81	2.5847446914392365E-4	0.001510460179059804	0.0	0.20487332610520248	4.846396296448569E-5
82-83	2.5847446914392365E-4	0.001530653496961673	0.0	0.2055195122780623	4.846396296448569E-5
84-85	2.5847446914392365E-4	0.0015589241420242896	0.0	0.2061980077595651	4.846396296448569E-5
86-87	2.705904598850451E-4	0.0015750787963457848	0.0	0.20646859821945016	4.846396296448569E-5
88-89	2.827064506261665E-4	0.0016235427593102706	0.0	0.20657764213612026	4.846396296448569E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13005	0.0	43.17843	1
GTATCAA	21565	0.0	33.76237	1
ATCAACG	25520	0.0	27.537113	3
TCAACGC	25630	0.0	27.467432	4
CAACGCA	26115	0.0	26.989124	5
TATCAAC	27355	0.0	26.251781	2
AACGCAG	27285	0.0	25.968704	6
ACGCAGA	31790	0.0	22.197014	7
CGCAGAG	32395	0.0	21.731188	8
GTACATG	35605	0.0	21.54549	1
TACATGG	35000	0.0	21.360182	2
ACATGGG	35520	0.0	20.345812	3
CATGGGG	26115	0.0	18.512281	4
GCAGAGT	39340	0.0	17.85258	9
GAGTACT	20710	0.0	16.83554	12-13
AGAGTAC	34830	0.0	16.777561	10-11
CAGAGTA	38065	0.0	16.38824	10-11
TATACCG	1070	0.0	16.302813	5
GTGGTAT	7170	0.0	16.161644	1
TGGTATC	6690	0.0	15.33456	2
>>END_MODULE