FastQCFastQC Report
Fri 27 May 2016
SRR2139983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33218498
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA457400.052.2254831
GTATCAA794200.033.6266861
ATCAACG1029550.025.4193923
TCAACGC1034750.025.2956474
CAACGCA1051500.024.8966275
TATCAAC1072600.024.6401562
AACGCAG1079000.024.3732156
ACGCAGA1247050.021.024297
CGCAGAG1253800.020.884728
GTGGTAT189100.019.7190321
AGAGTAC1308350.018.25944910-11
TGGTATC191450.018.1583422
GAGTACT926600.018.13341512-13
GCAGAGT1425900.018.0076279
CAGAGTA1404200.017.39588210-11
GTACATG890900.017.1740531
TACATGG875200.017.1416652
AGTACTT1011250.016.43149412-13
ACATGGG893900.016.326563
GTACTTT1047100.016.03896914-15
Produced by FastQC (version 0.11.5)