##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33218498
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98138892372557	31.0	31.0	33.0	28.0	34.0
2	31.15931232050287	31.0	31.0	34.0	28.0	34.0
3	31.176910075825823	31.0	31.0	34.0	27.0	34.0
4	35.03680717291914	35.0	35.0	37.0	32.0	37.0
5	34.796206017502655	35.0	35.0	37.0	32.0	37.0
6	34.857552499815014	35.0	35.0	37.0	32.0	37.0
7	34.74679418076037	35.0	35.0	37.0	31.0	37.0
8	34.7199445622135	35.0	35.0	37.0	30.0	37.0
9	36.20701583196206	38.0	35.0	39.0	32.0	39.0
10-11	36.07528446951454	38.0	35.0	39.0	30.5	39.0
12-13	36.01202637157165	37.0	35.0	39.0	30.0	39.0
14-15	36.95234012386713	38.0	36.0	40.0	31.0	41.0
16-17	36.91580757203411	38.0	36.0	40.0	31.0	41.0
18-19	36.94305966211958	38.5	36.0	40.0	31.0	41.0
20-21	36.918581866605635	39.0	36.0	40.0	31.0	41.0
22-23	36.81146918100428	39.0	35.5	40.0	30.5	41.0
24-25	36.679964947293	38.0	35.0	40.0	30.0	41.0
26-27	36.40921519133006	38.0	35.0	40.0	30.0	41.0
28-29	36.20459877289434	38.0	34.5	40.0	29.5	41.0
30-31	36.004435881936224	38.0	34.0	40.0	29.0	41.0
32-33	35.76784369532533	38.0	34.0	40.0	28.5	41.0
34-35	35.57961333503977	38.0	34.0	40.0	27.0	41.0
36-37	35.400378597836806	38.0	34.0	40.0	27.0	41.0
38-39	35.207016030601984	38.0	33.0	40.0	27.0	41.0
40-41	35.0410370223461	37.5	33.0	40.0	26.5	41.0
42-43	34.85603802811973	37.0	33.0	40.0	26.0	40.5
44-45	34.65675243558697	37.0	33.0	39.5	26.0	40.0
46-47	34.95840404685437	37.5	33.0	39.5	26.5	40.5
48-49	35.36402065705162	38.0	33.0	40.0	27.0	41.0
50-51	35.377138363088136	38.0	33.0	40.0	27.0	41.0
52-53	35.26938221509353	38.0	33.0	40.0	27.0	41.0
54-55	35.14122120925394	37.0	33.0	40.0	27.0	41.0
56-57	34.982019514329274	37.0	33.0	40.0	26.5	41.0
58-59	34.7921381205237	37.0	33.0	40.0	26.0	41.0
60-61	34.592037607327285	37.0	32.0	39.0	26.0	41.0
62-63	34.36298748183072	36.0	32.0	39.0	26.0	40.0
64-65	34.047965466430284	36.0	31.5	39.0	26.0	40.0
66-67	33.77224024736153	35.5	31.0	39.0	26.0	40.0
68-69	33.50278284044727	35.0	31.0	38.0	25.5	40.0
70-71	33.2310900127103	35.0	31.0	38.0	25.0	40.0
72-73	32.9353982975624	34.5	30.5	37.5	25.0	39.5
74-75	32.599289648053315	34.0	30.0	37.0	25.0	39.0
76-77	31.025681663923308	32.5	28.5	35.5	23.0	38.0
78-79	32.1429666808868	34.0	30.0	36.0	25.0	39.0
80-81	32.24083007808269	34.0	30.0	36.0	25.0	38.0
82-83	32.01557589282923	34.0	30.0	35.5	25.0	37.0
84-85	31.796366876447458	34.0	30.0	35.0	25.0	37.0
86-87	31.579695965580896	34.0	30.0	35.0	25.0	36.0
88-89	31.385378024605235	34.0	30.0	35.0	25.0	36.0
90-91	31.286509021963184	33.5	30.0	35.0	25.0	36.0
92-93	31.247851841079395	33.0	30.0	35.0	25.0	35.0
94-95	31.27900216385104	33.0	30.0	35.0	25.0	35.0
96-97	31.433493984219133	33.0	30.0	35.0	25.0	35.0
98-99	31.70921666185606	33.0	30.0	35.0	25.5	35.0
100	30.07411568480442	31.0	27.0	34.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	2.0
16	0.0
17	2.0
18	9.0
19	118.0
20	933.0
21	5599.0
22	25359.0
23	79627.0
24	189975.0
25	367944.0
26	603618.0
27	875686.0
28	1154190.0
29	1423784.0
30	1674953.0
31	1927625.0
32	2217191.0
33	2562047.0
34	3004784.0
35	3567168.0
36	4288607.0
37	4720312.0
38	3469286.0
39	1046880.0
40	12796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.3484918553512	23.329618937015155	16.050791941285244	22.271097266348406
2	15.348126909082517	23.994050891911176	39.89474909907247	20.763073099933845
3	18.149625548993818	28.309675530784084	32.190471104382866	21.350227815839233
4	13.107508726915796	20.556859079764422	39.10582460481201	27.229807588507775
5	12.106628060064606	39.285773848052976	36.591675517658864	12.015922574223554
6	28.05122916755598	40.861212328143196	18.752497478964884	12.335061025335945
7	24.37692095530629	34.004168400389446	24.641144220307613	16.977766423996655
8	23.466434274060195	38.1508880985528	21.63243503664735	16.750242590739653
9	25.037932178631316	16.41717214306318	21.71723417476612	36.82766150353938
10-11	23.264895962484516	27.61140344154031	30.118211545868206	19.00548905010696
12-13	24.127614727824334	25.333988163681052	30.062074713760346	20.476322394734268
14-15	21.342927796345965	26.757460916107984	27.613250864024124	24.286360423521927
16-17	20.695295454787317	29.75527042223752	28.326609132613406	21.22282499036176
18-19	20.73992779565169	28.76641803611951	30.170751549332543	20.322902618896254
20-21	21.57651505423819	27.977842283313308	30.04541463339	20.4002280290585
22-23	21.28840705402095	27.940979864594123	29.899863144425282	20.870749936959644
24-25	21.113395963454977	28.077239960232664	29.9714007308836	20.837963345428758
26-27	21.1442490280225	28.182088144825684	29.81093037907594	20.862732448075878
28-29	21.118606644416424	28.281443633254817	29.790228068524215	20.809721653804548
30-31	21.394136005560426	28.128993407279573	29.685558191478677	20.79131239568132
32-33	21.282925118930844	28.302630280490792	29.530708434157187	20.883736166421173
34-35	21.34514495689303	28.285945915437587	29.594711879073206	20.774197248596177
36-37	21.29717280224369	28.2495070529929	29.58210094989044	20.87121919487297
38-39	21.218213764588935	28.30724461097862	29.57737306258362	20.89716856184882
40-41	21.449899991426378	28.241557945910994	29.368052760510032	20.940489302152592
42-43	21.376039414699967	28.360320517151937	29.13990387181386	21.123736196334235
44-45	21.47453802437253	28.512405610274516	28.8429993958163	21.17005696953665
46-47	21.55920100358884	28.458679953734716	28.54961530060793	21.43250374206852
48-49	21.463105593090877	28.59568995411863	28.578706503751206	21.36249794903929
50-51	21.464963227894447	28.72273663364294	28.386439223224496	21.425860915238122
52-53	21.531454334722564	28.65453915844811	28.351675175836277	21.462331330993045
54-55	21.591176585501476	28.515767995966197	28.4211962893273	21.47185912920503
56-57	21.586715689257158	28.561554040057946	28.340264124833382	21.511466145851514
58-59	21.51066156173151	28.632325828708822	28.399929763879232	21.457082845680436
60-61	21.586935162069334	28.672440007430378	28.35818446865275	21.38244036184754
62-63	21.517875665808617	28.731315466896795	28.285573478821608	21.465235388472983
64-65	21.503916504887346	28.72518646725224	28.249822324784763	21.521074703075655
66-67	21.54974333225316	28.791879908023166	28.066507854268597	21.591868905455076
68-69	21.526332977473224	28.770600816632907	28.152212737959566	21.5508534679343
70-71	21.55352564612248	28.47586441759381	28.240494816581908	21.73011511970181
72-73	21.61749137753095	28.492205696624207	28.19936342888198	21.690939496962862
74-75	21.495944594570346	28.641299221647316	28.14502559706391	21.717730586718428
76-77	21.626303271336013	28.56880387559765	28.132395419764684	21.672497433301654
78-79	21.63456012869056	28.63333654766657	28.051512923578798	21.68059040006407
80-81	21.591207787184274	28.574068169813387	28.11407299256746	21.720651050434878
82-83	21.652234011458333	28.521116161702103	28.243248341853942	21.583401484985618
84-85	21.76933935102862	28.514795714757895	28.16406738915478	21.5517975450587
86-87	21.556450062130754	28.542727598320866	28.298496759298718	21.602325580249662
88-89	21.65932483404321	28.504441808387384	28.29986101023242	21.53637234733699
90-91	21.677332773620975	28.495364131841892	28.439652048545945	21.387651045991188
92-93	21.682392173625	28.462494685869792	28.408645720482472	21.446467420022728
94-95	21.374820387821323	28.48986029066326	28.614139027467655	21.521180294047763
96-97	21.33278445620177	28.69326497406029	28.767121235145616	21.206829334592328
98-99	22.302341451246836	30.64618414902542	29.10340100118744	17.948073398540306
100	21.983048029155743	31.212434539970896	22.968871871823858	23.83564555904951
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9358.0
1	8155.5
2	11249.5
3	16384.0
4	20941.5
5	32734.5
6	44097.0
7	54484.5
8	62922.5
9	63451.5
10	57652.5
11	50009.0
12	51808.0
13	67367.0
14	109244.0
15	170862.0
16	219319.0
17	243153.0
18	251277.5
19	249273.5
20	239108.0
21	223180.5
22	210699.0
23	216501.5
24	247212.0
25	304022.0
26	379816.5
27	464024.0
28	556741.5
29	661729.5
30	760749.5
31	854675.0
32	953537.5
33	1042952.5
34	1124794.0
35	1190559.5
36	1250702.5
37	1301885.5
38	1311735.5
39	1303687.5
40	1309541.5
41	1311450.5
42	1303734.0
43	1301722.0
44	1296841.0
45	1291948.0
46	1285731.0
47	1274912.0
48	1256526.0
49	1224203.5
50	1189480.5
51	1143542.0
52	1078700.0
53	1007219.5
54	933887.5
55	855234.5
56	776903.5
57	699095.0
58	619283.5
59	541904.5
60	460646.0
61	378667.5
62	303388.0
63	238195.5
64	183836.0
65	141259.0
66	109170.0
67	85625.0
68	67722.0
69	51435.0
70	38786.0
71	29898.0
72	22684.5
73	17233.0
74	13141.0
75	10005.5
76	7569.5
77	5540.5
78	4062.5
79	3069.5
80	2265.0
81	1644.0
82	1243.5
83	913.5
84	678.0
85	519.5
86	398.5
87	310.5
88	219.5
89	158.5
90	127.5
91	99.0
92	78.5
93	48.5
94	23.0
95	14.0
96	11.0
97	9.0
98	4.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10344838589631596
3	0.0
4	1.2041483633606794E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.005274169831519775
14-15	7.270045743790101E-4
16-17	0.0016481780723499298
18-19	0.0
20-21	5.569758691107912E-5
22-23	1.8520210488365605E-4
24-25	2.8170158543610636E-4
26-27	3.9198553018583326E-5
28-29	0.009053447500440334
30-31	0.0025608247657695673
32-33	0.0069527814286069614
34-35	0.005836910868849816
36-37	0.008637053801583944
38-39	0.003172367879220097
40-41	3.096287640910889E-5
42-43	2.1998105042667743E-4
44-45	0.0014583857653989632
46-47	4.42761568244911E-4
48-49	4.9856694971706246E-5
50-51	0.0
52-53	2.7794378560776664E-5
54-55	9.887093511718472E-6
56-57	0.0
58-59	8.797906742927281E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.231906107787898E-4
68-69	7.547447903473838E-4
70-71	0.0013772031188686936
72-73	5.30943030252255E-4
74-75	1.9689010226026123E-4
76-77	0.0038636571554233174
78-79	0.001959143917183068
80-81	1.2236533830481042E-4
82-83	1.778771148996038E-5
84-85	4.588661791052508E-4
86-87	0.0010119648109661022
88-89	8.815144653585384E-5
90-91	2.1536233366624763E-6
92-93	2.2183803325676E-6
94-95	2.2998821908346567E-6
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2081.0
20-21	6788.0
22-23	12745.0
24-25	24944.0
26-27	30581.0
28-29	46565.0
30-31	83655.0
32-33	107870.0
34-35	153005.0
36-37	185488.0
38-39	210112.0
40-41	239697.0
42-43	285414.0
44-45	349645.0
46-47	320719.0
48-49	274834.0
50-51	245230.0
52-53	234231.0
54-55	257235.0
56-57	292419.0
58-59	305579.0
60-61	313625.0
62-63	328177.0
64-65	348278.0
66-67	375147.0
68-69	385000.0
70-71	392671.0
72-73	389621.0
74-75	401509.0
76-77	357368.0
78-79	406759.0
80-81	440689.0
82-83	451349.0
84-85	473738.0
86-87	521123.0
88-89	585862.0
90-91	658091.0
92-93	760959.0
94-95	941982.0
96-97	1679926.0
98-99	2534785.0
100-101	1.6803002E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.15323559670112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58964540236745	71.89336364766537
2	7.192812356987277	11.674399916189033
3	2.0525835572302427	4.997213910054622
4	0.8779562858663387	2.849959732420627
5	0.4358558446053081	1.768555602172686
6	0.2703912713315748	1.3165875927397699
7	0.1627657077841643	0.9246274681610468
8	0.10827514269033625	0.7029502531212232
9	0.06961323464917246	0.5084405308919457
>10	0.23571110543038798	2.882290138591125
>50	0.003455510072617827	0.1854706893285548
>100	8.134767065640964E-4	0.12344358725208478
>500	6.111478387050548E-5	0.034427510467407836
>1k	5.998949465171524E-5	0.1382694209445058
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.0103709084016985E-6	3.0103709084016985E-6
2	0.0	1.5051854542008492E-5	0.0	6.020741816803397E-6	6.020741816803397E-6
3	0.0	2.107259635881189E-5	0.0	9.031112725205095E-6	6.020741816803397E-6
4	0.0	6.622815998483737E-5	0.0	1.806222545041019E-5	1.5051854542008492E-5
5	0.0	6.923853089323907E-5	0.0	3.0103709084016984E-5	1.5051854542008492E-5
6	4.214519271762378E-5	6.923853089323907E-5	0.0	3.0103709084016984E-5	3.0103709084016984E-5
7	4.214519271762378E-5	6.923853089323907E-5	0.0	3.0103709084016984E-5	3.0103709084016984E-5
8	4.214519271762378E-5	7.525927271004246E-5	0.0	3.0103709084016984E-5	3.913482180922208E-5
9	4.214519271762378E-5	1.4750817451168323E-4	0.0	3.913482180922208E-5	4.5155563626025474E-5
10-11	4.214519271762378E-5	1.4750817451168323E-4	0.0	4.6660749080226325E-5	4.8165934534427177E-5
12-13	4.214519271762378E-5	1.4901335996588406E-4	0.0	6.472297453063652E-5	5.117630544282887E-5
14-15	4.214519271762378E-5	1.8513781086670444E-4	0.0	8.429038543524756E-5	7.826964361844416E-5
16-17	5.418667635123057E-5	1.89653367229307E-4	0.0	1.1890965088186709E-4	9.633186906885435E-5
18-19	5.418667635123057E-5	2.1373633449652058E-4	0.0	1.7911706904990106E-4	1.09878538156662E-4
20-21	5.418667635123057E-5	2.4082967267213587E-4	0.0	2.2728300358432823E-4	1.1890965088186709E-4
22-23	5.569186180543142E-5	2.814696799355588E-4	0.0	4.2596748353884034E-4	1.3847706178647814E-4
24-25	5.719704725963227E-5	3.145837599279775E-4	0.0	0.001089754268841415	1.5202373087428578E-4
26-27	5.719704725963227E-5	3.3114079992418684E-4	0.0	0.0025994552794048665	1.5653928723688833E-4
28-29	5.719704725963227E-5	3.341511708325885E-4	0.0	0.008821891947071177	1.5653928723688833E-4
30-31	5.8702232713833116E-5	3.6576006537080634E-4	0.0	0.012691723749821561	1.5804447269108916E-4
32-33	6.321778907643566E-5	3.702756217334089E-4	0.0	0.019811250948191576	1.7309632723309766E-4
34-35	6.622815998483737E-5	4.1392599990523353E-4	0.0	0.02844499471348765	2.0018966540871294E-4
36-37	6.622815998483737E-5	4.620919344396607E-4	0.0	0.043761761895435486	2.047052217713155E-4
38-39	6.622815998483737E-5	4.8466971625267345E-4	0.0	0.0859912449984945	2.107259635881189E-4
40-41	6.773334543903821E-5	5.478875053291091E-4	0.0	0.1277526154252971	2.1373633449652058E-4
42-43	6.923853089323907E-5	5.539082471459126E-4	0.0	0.15673345616048023	2.1373633449652058E-4
44-45	7.375408725584162E-5	6.126104798597457E-4	0.0	0.19590590760605733	2.1825189085912313E-4
46-47	7.826964361844416E-5	6.637867853025745E-4	0.0	0.23639840669496856	2.2577781813012737E-4
48-49	7.826964361844416E-5	6.68302341665177E-4	0.0	0.2725017849994301	2.3480893085533246E-4
50-51	8.730075634364926E-5	7.074371634743992E-4	0.0	0.308832446307476	2.4233485812633673E-4
52-53	9.031112725205095E-5	7.164682761996043E-4	0.0	0.39675333905825605	2.603970835767469E-4
54-55	9.031112725205095E-5	7.315201307416127E-4	0.0	0.5020079474996131	2.754489381187554E-4
56-57	9.332149816045265E-5	7.435616143752195E-4	0.0	0.6066439247192934	2.814696799355588E-4
58-59	9.332149816045265E-5	7.450667998294203E-4	0.0	0.6675602852362561	2.874904217523622E-4
60-61	9.633186906885435E-5	7.571082834630272E-4	0.0	0.7137273334875045	2.905007926607639E-4
62-63	1.09878538156662E-4	7.67644581642433E-4	0.0	0.7479687973851196	3.0404746174857156E-4
64-65	1.1439409451926454E-4	8.203260725394628E-4	0.0	0.7562653796086747	3.0404746174857156E-4
66-67	1.1589927997346539E-4	8.218312579936636E-4	0.0	0.7629694756216852	3.1006820356537494E-4
68-69	1.1740446542766623E-4	8.398934834440738E-4	0.0	0.7696916338601463	3.175941308363792E-4
70-71	1.2041483633606793E-4	8.564505234402832E-4	0.0	0.7753857504333881	3.3114079992418684E-4
72-73	1.3245631996967473E-4	8.639764507112874E-4	0.0	0.7800442994141397	3.642548799166055E-4
74-75	1.3245631996967473E-4	8.985957161579069E-4	0.0	0.783703405253302	3.642548799166055E-4
76-77	1.339615054238756E-4	9.332149816045265E-4	0.0	0.7868823569325741	3.642548799166055E-4
78-79	1.3546669087807642E-4	9.48266836146535E-4	0.0	0.7892740966192993	3.642548799166055E-4
80-81	1.3697187633227728E-4	9.512772070549367E-4	0.0	0.7901636612227321	3.702756217334089E-4
82-83	1.3847706178647814E-4	9.527823925091375E-4	0.0	0.7910637621243441	3.9736895990902417E-4
84-85	1.3847706178647814E-4	9.693394325053469E-4	0.0	0.7915469266551426	3.9736895990902417E-4
86-87	1.3847706178647814E-4	9.813809161389536E-4	0.0	0.7918133444805362	3.9736895990902417E-4
88	1.4449780360328152E-4	0.0010024535124977655	0.0	0.7918299015205323	4.0640007263422926E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	45740	0.0	52.225483	1
GTATCAA	79420	0.0	33.626686	1
ATCAACG	102955	0.0	25.419392	3
TCAACGC	103475	0.0	25.295647	4
CAACGCA	105150	0.0	24.896627	5
TATCAAC	107260	0.0	24.640156	2
AACGCAG	107900	0.0	24.373215	6
ACGCAGA	124705	0.0	21.02429	7
CGCAGAG	125380	0.0	20.88472	8
GTGGTAT	18910	0.0	19.719032	1
AGAGTAC	130835	0.0	18.259449	10-11
TGGTATC	19145	0.0	18.158342	2
GAGTACT	92660	0.0	18.133415	12-13
GCAGAGT	142590	0.0	18.007627	9
CAGAGTA	140420	0.0	17.395882	10-11
GTACATG	89090	0.0	17.174053	1
TACATGG	87520	0.0	17.141665	2
AGTACTT	101125	0.0	16.431494	12-13
ACATGGG	89390	0.0	16.32656	3
GTACTTT	104710	0.0	16.038969	14-15
>>END_MODULE