FastQCFastQC Report
Fri 27 May 2016
SRR2139982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25064795
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA310350.050.7406351
GTATCAA555300.034.1166951
ATCAACG693950.026.5883483
TCAACGC693900.026.5783084
CAACGCA707450.026.1220195
AACGCAG723000.025.6921586
TATCAAC729500.025.5258162
ACGCAGA825450.022.4157147
CGCAGAG836350.022.1285788
GCAGAGT973400.018.7361749
GTACATG659600.018.4722081
GAGTACT616950.018.35201512-13
TACATGG647200.018.3320522
AGAGTAC888900.018.31030510-11
GTGGTAT138350.018.2500361
ACATGGG649600.017.7586423
CAGAGTA954050.017.56438310-11
TGGTATC139000.016.6708872
AGTACTT678250.016.38751412-13
GTACTTT711150.015.87657814-15
Produced by FastQC (version 0.11.5)