##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25064795
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232649100062456	31.0	31.0	34.0	30.0	34.0
2	31.40781618201944	31.0	31.0	34.0	30.0	34.0
3	31.440425425382493	31.0	31.0	34.0	28.0	34.0
4	35.27293560549767	37.0	35.0	37.0	33.0	37.0
5	35.04394733729121	37.0	35.0	37.0	32.0	37.0
6	35.08173711374859	37.0	35.0	37.0	32.0	37.0
7	34.967802609197484	37.0	35.0	37.0	32.0	37.0
8	34.941840856867174	37.0	35.0	37.0	32.0	37.0
9	36.454875573488636	38.0	35.0	39.0	32.0	39.0
10-11	36.351075362874504	38.0	35.0	39.0	32.0	39.0
12-13	36.28450932872182	38.0	35.0	39.0	32.0	39.0
14-15	37.300777445018	39.0	36.0	41.0	32.0	41.0
16-17	37.278124417135665	39.0	36.0	41.0	32.0	41.0
18-19	37.30602356811616	39.0	36.0	41.0	31.5	41.0
20-21	37.28750575878711	39.0	36.0	41.0	31.0	41.0
22-23	37.19253696218941	39.0	36.0	40.5	31.0	41.0
24-25	37.07869044981045	39.0	36.0	40.0	31.0	41.0
26-27	36.84450075794312	39.0	36.0	40.0	30.5	41.0
28-29	36.66192160702589	39.0	35.0	40.0	30.0	41.0
30-31	36.4894572690496	38.0	35.0	40.0	30.0	41.0
32-33	36.27943469132967	38.0	35.0	40.0	30.0	41.0
34-35	36.10883787757136	38.0	34.5	40.0	29.5	41.0
36-37	35.95650192932076	38.0	34.0	40.0	29.0	41.0
38-39	35.779947372614544	38.0	34.0	40.0	28.5	41.0
40-41	35.63378034642432	38.0	34.0	40.0	28.0	41.0
42-43	35.472629234564806	38.0	33.5	40.0	27.0	41.0
44-45	35.300121085723646	38.0	33.0	40.0	27.0	41.0
46-47	35.60180020807893	38.0	33.5	40.0	27.5	41.0
48-49	35.99131330574808	38.0	34.0	40.0	29.0	41.0
50-51	36.00534054080978	38.0	34.0	40.0	28.5	41.0
52-53	35.916299301725054	38.0	34.0	40.0	28.0	41.0
54-55	35.804377371641564	38.0	34.0	40.0	28.0	41.0
56-57	35.65893341277986	38.0	34.0	40.0	28.0	41.0
58-59	35.484031534410335	38.0	33.5	40.0	28.0	41.0
60-61	35.30065536551115	37.0	33.0	40.0	28.0	41.0
62-63	35.089861747046285	37.0	33.0	40.0	27.0	41.0
64-65	34.796504071314246	36.5	32.5	39.5	26.5	41.0
66-67	34.53507420959373	36.0	32.0	39.0	26.0	40.5
68-69	34.27760245433424	36.0	32.0	39.0	26.0	40.0
70-71	34.012517039682585	35.0	32.0	39.0	26.0	40.0
72-73	33.727108386653725	35.0	31.0	38.0	26.0	40.0
74-75	33.40286934136131	35.0	31.0	37.5	26.0	39.5
76-77	31.702159113101846	33.0	29.5	35.5	24.0	38.5
78-79	32.79308195505091	34.0	31.0	36.0	26.0	39.0
80-81	32.8714812436163	34.0	31.0	36.0	26.0	39.0
82-83	32.64146564859981	34.0	31.0	36.0	26.0	37.5
84-85	32.415126086783204	34.0	31.0	35.5	26.0	37.0
86-87	32.197764584804034	34.0	31.0	35.0	26.0	37.0
88-89	32.008588992222684	34.0	31.0	35.0	26.0	36.0
90-91	31.90157419439733	34.0	31.0	35.0	25.5	36.0
92-93	31.84815122236943	34.0	31.0	35.0	25.0	36.0
94-95	31.854733745087206	34.0	31.0	35.0	26.0	35.0
96-97	31.963546338583967	34.0	31.0	35.0	26.0	35.0
98-99	32.17426703004338	34.0	31.0	35.0	26.5	35.0
100	30.59763660079051	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	8.0
19	55.0
20	496.0
21	3129.0
22	14087.0
23	46260.0
24	112120.0
25	219303.0
26	366517.0
27	539357.0
28	720944.0
29	902332.0
30	1076291.0
31	1259166.0
32	1466832.0
33	1728015.0
34	2071522.0
35	2516810.0
36	3185191.0
37	3920749.0
38	3512501.0
39	1368689.0
40	34417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.04651923145591	22.44625579423251	15.356969805657696	23.15025516865388
2	14.530969060278833	23.028925967614494	41.091429340414024	21.34867563169265
3	17.462093745430593	27.98710701603584	32.006956370478996	22.543842868054575
4	12.309843287317781	19.80823136458331	39.86634316900439	28.015582179094523
5	12.06996905420531	39.02586476370543	36.75785898109281	12.146307200996457
6	28.028415951536807	40.51270317590868	19.09314239354441	12.365738479010101
7	24.44306446551827	33.83324300079055	24.712362498875414	17.011330034815764
8	23.290212427430586	38.10471220690215	21.82307894399296	16.781996421674307
9	24.892994337276647	16.662877154989697	21.913572403045787	36.530556104687875
10-11	23.11288203234856	27.56717938447133	30.280143922980418	19.039794660199693
12-13	24.017006730658856	25.24546478604175	30.136861877039607	20.600666606259786
14-15	21.234423508998642	26.705766276481807	27.64638914262198	24.413421071897574
16-17	20.687149893156857	29.754350338666775	28.32353314451507	21.2349666236613
18-19	20.679929758053078	28.86748126206498	30.060104221877737	20.392484758004205
20-21	21.54461641145062	28.072711137271156	29.884578422107204	20.49809402917102
22-23	21.21588057393996	27.99793557686868	29.784014768227795	21.002169080963558
24-25	21.026545997404558	28.199839495519935	29.831496644792054	20.942117862283453
26-27	21.054055505668597	28.349811343796272	29.66093415915168	20.93519899138345
28-29	21.02573078837776	28.422874610889302	29.658688189628112	20.892706411104825
30-31	21.38878307597033	28.19233592848193	29.557944640389678	20.860936355158064
32-33	21.18003839158505	28.37981483720901	29.47250150066577	20.967645270540167
34-35	21.248313776213593	28.364685926135717	29.48400524503551	20.90299505261518
36-37	21.211775312608342	28.367998474964207	29.464589765230304	20.955636447197147
38-39	21.102828057317826	28.42261679801951	29.487333752896383	20.987221391766283
40-41	21.331074412074916	28.309690636794638	29.362938660153056	20.996296290977398
42-43	21.282610572743188	28.41534112036989	29.115342791052818	21.186705515834102
44-45	21.36916382199913	28.591343624747605	28.84484114746657	21.194651405786693
46-47	21.452304287522004	28.492993839112724	28.569627335505704	21.48507453785957
48-49	21.39814890933831	28.605373095431148	28.59836746124686	21.39811053398368
50-51	21.33653111865111	28.726360829419995	28.49454383634172	21.44256421558718
52-53	21.45033739957391	28.62809774370641	28.43947398859533	21.482090868124352
54-55	21.471566368851715	28.536641170007123	28.480166396567864	21.5116260645733
56-57	21.491049255944613	28.584440408126877	28.407970404885198	21.51653993104331
58-59	21.404563672175307	28.668424883233456	28.46792182721491	21.45908961737632
60-61	21.473328697707355	28.714329076417023	28.413314987094022	21.3990272387816
62-63	21.375928190086775	28.79638220295756	28.37485570082418	21.45283390613148
64-65	21.3946566788845	28.76757452994065	28.296808255321903	21.540960535852946
66-67	21.458327073654125	28.830715215224657	28.129409871217142	21.581547839904076
68-69	21.438986972924255	28.798807880305528	28.187615526060732	21.57458962070948
70-71	21.4222896839056	28.487113879469746	28.314900539532452	21.775695897092202
72-73	21.495747384554363	28.49957938502324	28.28550761923197	21.719165611190427
74-75	21.397444474814943	28.654507262235107	28.201524874274487	21.74652338867546
76-77	21.5475517198736	28.59242372191225	28.154321308044473	21.705703250169684
78-79	21.510328629876906	28.632458221412175	28.1174304120422	21.739782736668715
80-81	21.505233539529296	28.59827816004559	28.124568947003	21.771919353422106
82-83	21.55626859443264	28.566427687040484	28.26489543125427	21.612408287272604
84-85	21.649849649570985	28.536860154756695	28.22626189371053	21.587028301961787
86-87	21.441514035796263	28.616433669164948	28.312598006935595	21.629454288103194
88-89	21.5140740778245	28.594570118115996	28.304810987221135	21.586544816838373
90-91	21.586080299227525	28.525941986778125	28.435374969240033	21.45260274475431
92-93	21.63021312708687	28.41070030920911	28.42526335515364	21.533823208550384
94-95	21.3493089424537	28.44344454150553	28.62782982088879	21.579416695151984
96-97	21.270863143519836	28.62706209701394	28.79636909559473	21.30570566387149
98-99	22.292310117439797	30.565083105925606	29.10179660660401	18.04081017003058
100	21.819754017321372	31.186579551962428	22.95774509345298	24.035921337263222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5641.0
1	5044.5
2	7309.5
3	10739.0
4	14150.0
5	23048.0
6	31291.5
7	37493.5
8	41621.0
9	40882.5
10	36952.0
11	32747.5
12	35069.0
13	46327.5
14	74697.0
15	115773.0
16	148627.5
17	166234.5
18	173263.5
19	172481.5
20	166658.5
21	157829.0
22	153151.5
23	162638.5
24	188145.0
25	231643.5
26	291117.5
27	357198.5
28	425207.0
29	501858.5
30	574914.5
31	643082.0
32	714570.0
33	779278.0
34	841174.0
35	891946.0
36	938976.5
37	978915.5
38	990734.0
39	994784.0
40	1008336.5
41	1014716.0
42	1008633.5
43	1004675.0
44	1002159.0
45	1001132.5
46	996576.5
47	987522.5
48	968597.5
49	936354.0
50	905370.5
51	867133.5
52	812365.0
53	750791.5
54	690467.0
55	633079.0
56	576920.5
57	518223.0
58	458344.5
59	399396.0
60	336389.0
61	274249.0
62	218913.5
63	171117.5
64	131052.0
65	99386.0
66	76053.5
67	59567.5
68	47005.5
69	35550.0
70	26847.0
71	20551.0
72	15511.5
73	11616.5
74	8726.5
75	6682.5
76	5233.5
77	3862.5
78	2745.5
79	1980.5
80	1430.0
81	1052.5
82	789.5
83	565.0
84	392.5
85	278.5
86	217.5
87	175.5
88	125.5
89	86.0
90	57.0
91	37.5
92	27.5
93	18.0
94	13.5
95	14.0
96	14.0
97	14.5
98	8.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11950626366583089
3	0.0
4	3.191727680198461E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.005681275270753262
14-15	7.181387280446539E-4
16-17	0.001923015927319573
18-19	0.0
20-21	2.194485389884344E-5
22-23	1.7160263350979204E-4
24-25	4.131963248841738E-4
26-27	5.9921575839972166E-5
28-29	0.00938575857569964
30-31	0.0028492586971719555
32-33	0.007643038348458064
34-35	0.006578429211739568
36-37	0.00911147728451007
38-39	0.003143567971650762
40-41	4.1031490643281456E-5
42-43	2.6052660863705256E-4
44-45	0.0015133016817415518
46-47	6.712747780344454E-4
48-49	6.822280613363962E-5
50-51	0.0
52-53	2.8184826902235165E-5
54-55	2.185471192943603E-5
56-57	0.0
58-59	0.0010823560244893964
60-61	0.0
62-63	0.0
64-65	0.0
66-67	5.7530488189049E-4
68-69	0.0011491417786822307
70-71	0.001327183714006688
72-73	6.011173122610044E-4
74-75	1.9657242558677117E-4
76-77	0.004121188763127849
78-79	0.00212663462621353
80-81	1.589901071483055E-4
82-83	2.606177903020263E-5
84-85	4.5843182661631335E-4
86-87	0.001116752720466274
88-89	1.15570016360862E-4
90-91	2.8149131282622203E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1208.0
20-21	4036.0
22-23	7754.0
24-25	14454.0
26-27	19035.0
28-29	32110.0
30-31	62681.0
32-33	81772.0
34-35	114123.0
36-37	146708.0
38-39	164660.0
40-41	183796.0
42-43	215828.0
44-45	265048.0
46-47	245129.0
48-49	211893.0
50-51	188772.0
52-53	179945.0
54-55	196891.0
56-57	220798.0
58-59	231576.0
60-61	239750.0
62-63	242593.0
64-65	258047.0
66-67	277077.0
68-69	279368.0
70-71	282239.0
72-73	278475.0
74-75	288187.0
76-77	262560.0
78-79	292679.0
80-81	311853.0
82-83	315389.0
84-85	325621.0
86-87	355785.0
88-89	395526.0
90-91	440649.0
92-93	502568.0
94-95	614861.0
96-97	1158545.0
98-99	1837039.0
100-101	1.3317767E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.4910245864504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.83925922616093	73.28441517067282
2	7.2393834524391965	11.943683167318078
3	2.0152218099772274	4.987131356219475
4	0.8123394690608975	2.6804286045938626
5	0.41012705674528277	1.691590056077183
6	0.21975074803822686	1.0876478615587342
7	0.1423644239148824	0.8220651035378888
8	0.08355956656274399	0.5514331407808363
9	0.05618838437125758	0.4171533656978101
>10	0.1785046482763878	2.2002186332381264
>50	0.00261785494344986	0.1411847274057657
>100	6.024803903345087E-4	0.08720081467364446
>500	3.60693959537333E-5	0.02211091084251319
>1k	4.4809723243176364E-5	0.08373708738327307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9948298001240386E-5	0.0	0.0	0.0
3	0.0	2.3937957601488463E-5	0.0	0.0	0.0
4	0.0	3.989659600248077E-5	0.0	0.0	7.979319200496155E-6
5	3.989659600248077E-6	4.7875915202976925E-5	0.0	0.0	7.979319200496155E-6
6	7.979319200496155E-6	4.7875915202976925E-5	0.0	0.0	1.1968978800744231E-5
7	7.979319200496155E-6	5.1865574803225E-5	0.0	0.0	1.1968978800744231E-5
8	1.1968978800744231E-5	6.383455360396924E-5	0.0	0.0	1.1968978800744231E-5
9	1.595863840099231E-5	1.1968978800744231E-4	0.0	0.0	1.9948298001240386E-5
10-11	3.5906936402232696E-5	1.3165876680818655E-4	0.0	5.9844894003721165E-6	1.9948298001240386E-5
12-13	3.5906936402232696E-5	1.4163291580880673E-4	0.0	9.974149000620193E-6	1.9948298001240386E-5
14-15	3.790176620235673E-5	2.074622992129E-4	0.0	1.595863840099231E-5	4.388625560272885E-5
16-17	3.989659600248077E-5	2.61322703816249E-4	0.0	3.790176620235673E-5	5.785006420359712E-5
18-19	4.388625560272885E-5	2.7728134221724135E-4	0.0	6.18397238038452E-5	6.782421320421731E-5
20-21	4.388625560272885E-5	2.992244700186058E-4	0.0	1.1171046880694614E-4	7.181387280446539E-5
22-23	4.388625560272885E-5	3.271520872203423E-4	0.0	3.3114174682059036E-4	9.774666020607789E-5
24-25	5.1865574803225E-5	3.790176620235673E-4	0.0	0.0010173631980632597	1.1171046880694616E-4
26-27	5.1865574803225E-5	3.8699698122406345E-4	0.0	0.0027568547837714215	1.1570012840719424E-4
28-29	5.1865574803225E-5	3.9298147062443556E-4	0.0	0.010434954684448845	1.1968978800744231E-4
30-31	5.585523440347308E-5	4.229039176262962E-4	0.0	0.013824170514859586	1.2168461780756635E-4
32-33	5.9844894003721155E-5	4.3287806662691637E-4	0.0	0.020724286793488637	1.3963808600868269E-4
34-35	6.18397238038452E-5	4.8075398182989326E-4	0.0	0.02802137420234237	1.4761740520917885E-4
36-37	6.582938340409327E-5	5.266350672327462E-4	0.0	0.04148647535317963	1.4761740520917885E-4
38-39	6.981904300434134E-5	5.425937056337385E-4	0.0	0.08083249833082616	1.5360189460955094E-4
40-41	7.580353240471346E-5	6.124127486380798E-4	0.0	0.12035606116068374	1.595863840099231E-4
42-43	8.378285160520961E-5	6.423351956399404E-4	0.0	0.14535127855623795	1.595863840099231E-4
44-45	8.577768140533365E-5	7.221283876449019E-4	0.0	0.17994761177978913	1.6158121381004714E-4
46-47	9.176217080570577E-5	7.879577710489952E-4	0.0	0.21496884375076675	1.6956053301054328E-4
48-49	9.575183040595385E-5	8.039164094499876E-4	0.0	0.24715941223536836	1.8352434161141154E-4
50-51	9.575183040595385E-5	8.81714771654825E-4	0.0	0.2785101573741178	1.8352434161141154E-4
52-53	9.575183040595385E-5	8.976734100558173E-4	0.0	0.3606333105856242	2.1344678861327213E-4
54-55	9.575183040595385E-5	9.076475590564376E-4	0.0	0.46080967348825314	2.4536406541525674E-4
56-57	9.575183040595385E-5	9.116372186566856E-4	0.0	0.5580496469250995	2.493537250155048E-4
58-59	9.575183040595385E-5	9.256010272575539E-4	0.0	0.609617992088106	2.57333044216001E-4
60-61	9.575183040595385E-5	9.455493252587942E-4	0.0	0.6392970698543514	2.7129685281686923E-4
62-63	9.974149000620192E-5	9.774666020607788E-4	0.0	0.6607754023122869	2.8725549121786157E-4
64-65	9.974149000620192E-5	0.001091171900667849	0.0	0.6683697193613591	2.8725549121786157E-4
66-67	1.0173631980632596E-4	0.0011131150284692136	0.0	0.6748190041051603	2.8725549121786157E-4
68-69	1.0373114960645E-4	0.001182934071473555	0.0	0.6815874616169811	2.8725549121786157E-4
70-71	1.0772080920669807E-4	0.0012208358376759117	0.0	0.6866224120324942	2.952348104183577E-4
72-73	1.0772080920669807E-4	0.0012407841356771518	0.0	0.6906419940797441	3.311417468205904E-4
74-75	1.0772080920669807E-4	0.0012687117528788886	0.0	0.6937499389083374	3.311417468205904E-4
76-77	1.0772080920669807E-4	0.0013305514766827338	0.0	0.6967282197999225	3.3313657662071444E-4
78-79	1.0772080920669807E-4	0.001346510115083726	0.0	0.6988886204734569	3.3513140642083846E-4
80-81	1.1171046880694616E-4	0.0013564842640843462	0.0	0.6997045058617076	3.471003852215827E-4
82-83	1.1171046880694616E-4	0.0013664584130849665	0.0	0.7002810116739435	3.6305902362257503E-4
84-85	1.1171046880694616E-4	0.001388401540886331	0.0	0.7005722568247617	3.6305902362257503E-4
86-87	1.1171046880694616E-4	0.0014243084772885635	0.0	0.7007178794001706	3.6505385342269905E-4
88	1.1171046880694616E-4	0.0014761740520917886	0.0	0.7007358328683717	3.6704868322282307E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31035	0.0	50.740635	1
GTATCAA	55530	0.0	34.116695	1
ATCAACG	69395	0.0	26.588348	3
TCAACGC	69390	0.0	26.578308	4
CAACGCA	70745	0.0	26.122019	5
AACGCAG	72300	0.0	25.692158	6
TATCAAC	72950	0.0	25.525816	2
ACGCAGA	82545	0.0	22.415714	7
CGCAGAG	83635	0.0	22.128578	8
GCAGAGT	97340	0.0	18.736174	9
GTACATG	65960	0.0	18.472208	1
GAGTACT	61695	0.0	18.352015	12-13
TACATGG	64720	0.0	18.332052	2
AGAGTAC	88890	0.0	18.310305	10-11
GTGGTAT	13835	0.0	18.250036	1
ACATGGG	64960	0.0	17.758642	3
CAGAGTA	95405	0.0	17.564383	10-11
TGGTATC	13900	0.0	16.670887	2
AGTACTT	67825	0.0	16.387514	12-13
GTACTTT	71115	0.0	15.876578	14-15
>>END_MODULE