FastQCFastQC Report
Fri 27 May 2016
SRR2139981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6919806
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.052.2265241
GTATCAA140650.035.6027371
TCAACGC174900.028.0715774
ATCAACG176300.027.8727723
CAACGCA179500.027.304835
AACGCAG184500.026.6570226
TATCAAC186300.026.5821972
GACCGAG56450.026.3548690-91
GAGACCG57150.026.13545688-89
CGAGACC56350.025.50724888-89
AGACCGA57800.025.16030590-91
ACCGAGG59850.024.99058392-93
ACGAGAC59100.024.73441786-87
GCCCACG60350.023.38336882-83
CACGAGA60750.023.2015386-87
ACGCAGA217450.022.598167
CCACGAG63900.022.59124884-85
CCCACGA62000.022.5473284-85
CGCAGAG220300.022.34448
ATCTCCG62700.022.2514474-75
Produced by FastQC (version 0.11.5)