##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6919806
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945871603914906	31.0	31.0	34.0	30.0	34.0
2	32.116860357067814	33.0	31.0	34.0	30.0	34.0
3	32.17270700941616	34.0	31.0	34.0	30.0	34.0
4	35.84761119025591	37.0	35.0	37.0	35.0	37.0
5	35.720782634657674	37.0	35.0	37.0	33.0	37.0
6	35.74008534343304	37.0	35.0	37.0	33.0	37.0
7	35.62543082855213	37.0	35.0	37.0	33.0	37.0
8	35.6104111878281	37.0	35.0	37.0	33.0	37.0
9	37.18536661287903	39.0	37.0	39.0	33.0	39.0
10-11	37.096512315518666	39.0	37.0	39.0	33.0	39.0
12-13	37.05956864975694	39.0	37.0	39.0	33.0	39.0
14-15	38.227015251583644	40.0	38.0	41.0	33.0	41.0
16-17	38.22990052033251	40.0	38.0	41.0	33.0	41.0
18-19	38.2694726557363	40.0	38.0	41.0	33.0	41.0
20-21	38.24295112946075	40.0	38.0	41.0	33.0	41.0
22-23	38.15982039144973	40.0	37.5	41.0	33.0	41.0
24-25	38.08060760626815	40.0	37.0	41.0	33.0	41.0
26-27	37.90989339569268	40.0	37.0	41.0	33.0	41.0
28-29	37.804245353644916	40.0	37.0	41.0	32.5	41.0
30-31	37.677509177675766	40.0	37.0	41.0	32.0	41.0
32-33	37.850372816291	40.0	37.0	41.0	33.0	41.0
34-35	37.83790905536276	40.0	37.0	41.0	33.0	41.0
36-37	37.7571067197631	40.0	37.0	41.0	33.0	41.0
38-39	37.56810350395891	40.0	37.0	41.0	32.0	41.0
40-41	37.58576167993196	40.0	37.0	41.0	32.0	41.0
42-43	37.55222536534761	40.0	37.0	41.0	32.0	41.0
44-45	37.529140830522415	40.0	36.5	41.0	32.0	41.0
46-47	37.553835202336245	40.0	37.0	41.0	32.0	41.0
48-49	37.46833234336864	40.0	36.0	41.0	32.0	41.0
50-51	37.38622069818184	39.0	36.0	41.0	31.0	41.0
52-53	37.243464184874185	39.0	36.0	41.0	31.0	41.0
54-55	37.134150175069834	39.0	35.5	41.0	31.0	41.0
56-57	36.98734483444893	39.0	35.0	40.0	31.0	41.0
58-59	36.83684545923759	39.0	35.0	40.0	31.0	41.0
60-61	36.63599319659642	38.0	35.0	40.0	31.0	41.0
62-63	36.39243578126578	38.0	35.0	40.0	30.0	41.0
64-65	36.191968645353384	38.0	34.5	40.0	30.0	41.0
66-67	35.90627702398226	37.0	34.0	40.0	30.0	41.0
68-69	35.62375962370261	37.0	34.0	39.5	29.0	41.0
70-71	35.338487321244635	36.0	34.0	39.0	29.0	40.5
72-73	35.04049988567818	36.0	34.0	39.0	29.0	40.0
74-75	34.70983306669174	35.0	33.5	38.0	29.0	40.0
76-77	33.66967449843021	35.0	32.0	37.0	27.5	39.0
78-79	34.00861180230615	35.0	33.0	37.0	29.0	39.0
80-81	33.90503080291809	35.0	33.0	36.5	29.0	39.0
82-83	33.69574738733394	35.0	33.0	36.0	29.0	37.5
84-85	33.484494964755775	35.0	33.0	36.0	29.0	37.0
86-87	33.29238072521859	35.0	33.0	35.0	29.0	37.0
88-89	33.086716900125325	35.0	33.0	35.0	29.0	36.0
90-91	32.98712289728908	34.5	32.5	35.0	29.0	36.0
92-93	32.9312804050635	34.0	32.0	35.0	29.0	36.0
94-95	32.8831254094807	34.0	32.0	35.0	29.0	35.5
96-97	32.80418083680841	34.0	32.0	35.0	29.0	35.0
98-99	32.94326838907282	34.0	32.0	35.0	29.0	35.0
100-101	32.49141732613303	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	67.0
21	287.0
22	1486.0
23	5305.0
24	13395.0
25	27743.0
26	49528.0
27	76995.0
28	107637.0
29	139247.0
30	174622.0
31	215321.0
32	263072.0
33	331628.0
34	432968.0
35	592098.0
36	888332.0
37	1411364.0
38	1535012.0
39	632948.0
40	20740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.85208718279096	22.223310884727116	15.616319301437063	24.308282631044857
2	14.11166261206046	22.822138872454335	40.40236125775358	22.66383725773162
3	16.954254333540998	27.281210173179787	31.763115221632752	24.001420271646463
4	12.219143137827853	19.425501235150232	38.96947399970462	29.385881627317296
5	12.63268074278383	38.36152342999211	36.184627141281126	12.82116868594293
6	29.316732289893675	39.310668536083234	18.598180353611067	12.774418820412018
7	25.548331846297422	33.05734293707078	23.934760598779793	17.459564617852003
8	24.09466392554936	37.233298158936826	21.323690288427162	17.348347627086653
9	25.429600193993878	16.181537459287153	21.202949909289366	37.1859124374296
10-11	23.773245955161173	26.971529259635314	29.628605195001136	19.626619590202385
12-13	24.583774458416897	24.68426137958203	29.465826065065986	21.266138096935087
14-15	21.81436878432719	26.071959242788022	26.879611942878167	25.23406003000662
16-17	21.461223045848396	29.022128655051887	27.605961785633877	21.91068651346584
18-19	21.501029075092568	28.27239665389463	29.183730006303644	21.042844264709153
20-21	22.323997791976208	27.461201150201475	28.999331508440257	21.21546954938206
22-23	22.038605876656113	27.363295213770595	28.866341986844805	21.731756922728483
24-25	21.840253449793945	27.545945870927184	28.88281127513006	21.730989404148808
26-27	21.80429231056725	27.644517123016225	28.832783808505013	21.71840675791151
28-29	21.761960343604457	27.677083057517677	28.893565757853167	21.6673908410247
30-31	22.052009617896548	27.582741931967703	28.712755692202684	21.652492757933068
32-33	21.880600739293047	27.723757938247857	28.65722628146222	21.738415040996877
34-35	21.954983047491773	27.693243308162973	28.684240791126758	21.667532853218503
36-37	21.91796901482677	27.650629033228864	28.685701180017354	21.745700771927012
38-39	21.84131717917509	27.794925917608555	28.640167427109414	21.72358947610694
40-41	22.017579704145575	27.655836687623925	28.579340214235533	21.74724339399497
42-43	21.916708798129594	27.78782965247	28.34254961412745	21.95291193527296
44-45	22.02645699804551	27.86814960776892	28.15864524402497	21.946748150160595
46-47	22.108924415145232	27.79329734997205	27.93061086386234	22.167167371020373
48-49	22.00091931593952	27.91904630005192	27.963549827337335	22.116484556671217
50-51	22.004877044706088	28.054487818428907	27.79324072857341	22.147394408291593
52-53	22.190659348568953	27.908082057419342	27.68994754982883	22.211311044182878
54-55	22.154499085071134	27.806043235328985	27.76919672570263	22.270260953897246
56-57	22.135716192739313	27.900677707889677	27.72907595870486	22.23453014066614
58-59	22.092402794997852	27.945534561689893	27.762852825395612	22.199209817916646
60-61	22.110428345291762	27.809741808615367	27.849857309164545	22.229972536928326
62-63	22.28345684340226	27.857532631937726	27.819301492788284	22.03970903187173
64-65	22.003597567079023	27.833484785739003	27.787329419777002	22.375588227404968
66-67	22.09882835093723	27.926571138362384	27.611331535686922	22.363268975013465
68-69	22.04190653131875	28.101834677845623	27.660227721240748	22.19603106959488
70-71	22.060054698780085	27.860981133900246	27.747326161955964	22.331638005363697
72-73	22.058083756030435	27.881665336785037	27.51980924964743	22.540441657537094
74-75	21.977189555558173	27.951187123237798	27.463338688498943	22.60828463270509
76-77	22.130521871698367	27.667745725567567	27.607913480050783	22.593818922683287
78-79	22.070649542590193	27.747884523436074	27.557811141505468	22.623654792468265
80-81	22.354899299222303	27.703827725430642	27.307010141323335	22.63426283402372
82-83	22.32959631977597	27.850479232295	27.469164215012963	22.350760232916063
84-85	22.190193208863658	27.633985986074773	27.39518197407128	22.780638830990288
86-87	22.08100240352537	27.85310868942644	27.46681845511645	22.59907045193174
88-89	22.35986844297212	27.60577632449464	27.45744625152904	22.57690898100419
90-91	22.3944677002879	27.80760688307599	27.524069652836953	22.27385576379916
92-93	22.218476813058942	27.70247534091601	27.54113691303771	22.537910932987337
94-95	22.292446488462016	27.49594357712172	27.61569241939032	22.59591751502594
96-97	22.221417839882978	27.76860598314501	27.679243809916443	22.330732367055564
98-99	22.409074185996623	28.24632900544636	27.829298009542676	21.51529879901434
100-101	22.845472928308862	30.990348003619804	25.065383311339694	21.09879575673164
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1606.0
1	1420.0
2	1826.5
3	2539.0
4	3274.5
5	4939.5
6	6439.5
7	7912.5
8	8990.0
9	9100.0
10	8557.5
11	7942.5
12	8829.5
13	11732.0
14	18835.5
15	28693.0
16	35994.0
17	39054.0
18	39400.5
19	38097.0
20	35519.5
21	32989.0
22	32256.0
23	34912.5
24	41261.0
25	51320.0
26	64370.0
27	78563.5
28	93985.0
29	111068.5
30	127227.0
31	143361.0
32	161217.5
33	178984.5
34	194692.5
35	208180.0
36	223350.0
37	236142.0
38	244528.5
39	250958.5
40	258280.0
41	265716.0
42	268328.0
43	271395.5
44	275455.5
45	278163.0
46	279479.5
47	279058.0
48	277331.5
49	273530.0
50	268901.5
51	260958.5
52	248455.5
53	235982.0
54	222812.5
55	206121.5
56	188974.0
57	171703.5
58	153540.0
59	133831.0
60	112545.0
61	92592.0
62	74029.0
63	57409.0
64	43321.5
65	32611.0
66	24979.5
67	19538.0
68	15194.5
69	11270.5
70	8418.0
71	6386.0
72	4803.5
73	3554.0
74	2621.0
75	1921.5
76	1403.0
77	988.5
78	685.0
79	454.0
80	293.0
81	208.0
82	159.0
83	117.5
84	86.0
85	62.0
86	45.5
87	32.0
88	21.0
89	14.0
90	9.0
91	6.0
92	4.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015029323076398383
3	4.33538165665338E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.002485735383496581
22-23	3.468840955448881E-4
24-25	0.001098700215959647
26-27	0.012025134628360069
28-29	2.3873822939141068E-4
30-31	0.002737631680627019
32-33	0.0030265258661996866
34-35	0.0016449994999492672
36-37	0.05853180338839791
38-39	0.02734830295654348
40-41	0.02541989057610083
42-43	0.0030838758360078257
44-45	0.0016223927453807062
46-47	0.0034054169337656265
48-49	0.0028096225053978055
50-51	6.48538972945888E-4
52-53	9.41534152867485E-4
54-55	0.0012938062202909747
56-57	0.011544754585402559
58-59	0.008568082027658151
60-61	0.004340308768442601
62-63	0.0024379856633483603
64-65	0.001128520308704277
66-67	4.1319968031567136E-4
68-69	0.0012200785145594225
70-71	3.3815808839908944E-5
72-73	5.131349724574804E-4
74-75	1.2113134600632548E-4
76-77	0.0043085767650014655
78-79	0.001055410094225949
80-81	6.742437277802979E-4
82-83	5.468681499067681E-4
84-85	0.003058061312928273
86-87	0.0034110004389320344
88-89	0.041696275272300846
90-91	3.1930855956909215E-4
92-93	5.420957559914641E-4
94-95	2.2129094498576343E-4
96-97	0.016779055360866886
98-99	1.0817632351297851E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	207.0
20-21	616.0
22-23	1157.0
24-25	2366.0
26-27	3176.0
28-29	5337.0
30-31	13815.0
32-33	18079.0
34-35	25713.0
36-37	39477.0
38-39	40812.0
40-41	44399.0
42-43	52659.0
44-45	63159.0
46-47	63224.0
48-49	56284.0
50-51	51735.0
52-53	48217.0
54-55	52452.0
56-57	55176.0
58-59	34320.0
60-61	59805.0
62-63	58311.0
64-65	62324.0
66-67	66870.0
68-69	68407.0
70-71	68655.0
72-73	67115.0
74-75	69026.0
76-77	70757.0
78-79	75334.0
80-81	76408.0
82-83	74078.0
84-85	75088.0
86-87	79586.0
88-89	85522.0
90-91	93503.0
92-93	98145.0
94-95	122028.0
96-97	157792.0
98-99	598380.0
100-101	4120292.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.0504454102385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.17673453272849	82.08379266600784
2	5.785790686429109	10.304544753538433
3	1.2068970358542852	3.2242415582136204
4	0.40508062936273415	1.4429044188724487
5	0.1779836048399933	0.792475964336064
6	0.08088227891960037	0.4321561778151334
7	0.04781415361674701	0.2980510173539422
8	0.027422191105214655	0.1953566665635236
9	0.02246650325984514	0.18005869098898067
>10	0.06766320144384927	0.9320420724252548
>50	9.536814872519733E-4	0.05634235472659736
>100	2.8884419720127556E-4	0.04437454651145058
>500	2.265675570522927E-5	0.013659112646678025
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.44512721888446E-5	0.0	0.0	0.0
3	0.0	1.44512721888446E-5	0.0	0.0	0.0
4	0.0	5.78050887553784E-5	0.0	0.0	0.0
5	0.0	7.2256360944223E-5	0.0	0.0	0.0
6	7.2256360944223E-5	7.2256360944223E-5	0.0	0.0	1.44512721888446E-5
7	7.2256360944223E-5	7.2256360944223E-5	0.0	1.44512721888446E-5	1.44512721888446E-5
8	7.2256360944223E-5	7.2256360944223E-5	0.0	1.44512721888446E-5	1.44512721888446E-5
9	7.2256360944223E-5	1.300614496996014E-4	0.0	1.44512721888446E-5	1.44512721888446E-5
10-11	9.39332692274899E-5	1.300614496996014E-4	0.0	2.16769082832669E-5	1.44512721888446E-5
12-13	1.011589053219122E-4	1.372870857940237E-4	0.0	4.33538165665338E-5	1.44512721888446E-5
14-15	1.011589053219122E-4	1.8786653845497982E-4	0.0	4.33538165665338E-5	3.61281804721115E-5
16-17	1.44512721888446E-4	2.16769082832669E-4	0.0	5.7805088755378406E-5	5.78050887553784E-5
18-19	1.44512721888446E-4	2.3122035502151362E-4	0.0	7.2256360944223E-5	7.2256360944223E-5
20-21	1.44512721888446E-4	2.601228993992028E-4	0.0	1.156101775107568E-4	7.948199703864531E-5
22-23	1.5896399407729062E-4	3.0347671596573663E-4	0.0	3.251536242490035E-4	8.67076331330676E-5
24-25	1.734152662661352E-4	3.251536242490035E-4	0.0	7.297892455366524E-4	9.39332692274899E-5
26-27	1.734152662661352E-4	3.323792603434258E-4	0.0	0.0021749164644211125	1.083845414163345E-4
28-29	1.734152662661352E-4	3.323792603434258E-4	0.0	0.013172334600131855	1.156101775107568E-4
30-31	1.734152662661352E-4	3.323792603434258E-4	0.0	0.02018842724781591	1.156101775107568E-4
32-33	1.734152662661352E-4	3.323792603434258E-4	0.0	0.024776706167774068	1.300614496996014E-4
34-35	1.8786653845497982E-4	3.829587130043819E-4	0.0	0.031120814658676846	1.300614496996014E-4
36-37	1.8786653845497982E-4	4.190868934764934E-4	0.0	0.03966874215837843	1.300614496996014E-4
38-39	1.8786653845497982E-4	4.479894378541826E-4	0.0	0.05518218285310311	1.372870857940237E-4
40-41	1.8786653845497982E-4	5.05794526609561E-4	0.0	0.08737961728984887	1.44512721888446E-4
42-43	2.16769082832669E-4	5.419227070816725E-4	0.0	0.10230778146092535	1.5173835798286831E-4
44-45	2.239947189270913E-4	6.214047041203179E-4	0.0	0.1253575606021325	1.5896399407729062E-4
46-47	2.312203550215136E-4	6.792097928756963E-4	0.0	0.15052445111900536	1.734152662661352E-4
48-49	2.312203550215136E-4	6.864354289701185E-4	0.0	0.17293837428390332	1.8786653845497982E-4
50-51	2.601228993992028E-4	7.370148816310746E-4	0.0	0.19348085770034595	1.8786653845497982E-4
52-53	2.601228993992028E-4	7.370148816310746E-4	0.0	0.2213429104804383	1.9509217454940212E-4
54-55	2.601228993992028E-4	7.370148816310746E-4	0.0	0.2789673583334562	2.2399471892709132E-4
56-57	2.601228993992028E-4	7.44240517725497E-4	0.0	0.34308765303535965	2.4567162721035824E-4
58-59	2.745741715880474E-4	7.514661538199193E-4	0.0	0.396904190666617	2.601228993992028E-4
60-61	2.745741715880474E-4	7.514661538199193E-4	0.0	0.7656284005649869	2.601228993992028E-4
62-63	2.745741715880474E-4	7.586917899143415E-4	0.0	0.7751373376652467	2.601228993992028E-4
64-65	2.745741715880474E-4	8.526250591418314E-4	0.0	0.7852749051057212	2.601228993992028E-4
66-67	2.89025443776892E-4	8.743019674250983E-4	0.0	0.793006335726753	2.6734853549362507E-4
68-69	3.0347671596573663E-4	9.176557839916321E-4	0.0	0.8007883458004459	2.8179980768246974E-4
70-71	3.0347671596573663E-4	9.248814200860544E-4	0.0	0.8086642891433662	2.89025443776892E-4
72-73	3.323792603434258E-4	9.39332692274899E-4	0.0	0.8147771772792474	3.323792603434258E-4
74-75	3.396048964378481E-4	9.754608727470106E-4	0.0	0.8203120145275749	3.396048964378481E-4
76-77	3.468305325322704E-4	0.0010260403254079668	0.0	0.82518931889131	3.468305325322704E-4
78-79	3.468305325322704E-4	0.0010693941419745005	0.0	0.830066623255045	3.468305325322704E-4
80-81	3.468305325322704E-4	0.0010693941419745005	0.0	0.8331375185951745	3.685074408155373E-4
82-83	3.468305325322704E-4	0.001083845414163345	0.0	0.8351173428850462	3.7573307690995963E-4
84-85	3.61281804721115E-4	0.001083845414163345	0.0	0.8368442699116132	3.829587130043819E-4
86-87	3.61281804721115E-4	0.001083845414163345	0.0	0.8371405209914844	3.901843490988042E-4
88-89	3.829587130043819E-4	0.0011705530472964126	0.0	0.8371766491719566	4.046356212876488E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7830	0.0	52.226524	1
GTATCAA	14065	0.0	35.602737	1
TCAACGC	17490	0.0	28.071577	4
ATCAACG	17630	0.0	27.872772	3
CAACGCA	17950	0.0	27.30483	5
AACGCAG	18450	0.0	26.657022	6
TATCAAC	18630	0.0	26.582197	2
GACCGAG	5645	0.0	26.35486	90-91
GAGACCG	5715	0.0	26.135456	88-89
CGAGACC	5635	0.0	25.507248	88-89
AGACCGA	5780	0.0	25.160305	90-91
ACCGAGG	5985	0.0	24.990583	92-93
ACGAGAC	5910	0.0	24.734417	86-87
GCCCACG	6035	0.0	23.383368	82-83
CACGAGA	6075	0.0	23.20153	86-87
ACGCAGA	21745	0.0	22.59816	7
CCACGAG	6390	0.0	22.591248	84-85
CCCACGA	6200	0.0	22.54732	84-85
CGCAGAG	22030	0.0	22.3444	8
ATCTCCG	6270	0.0	22.25144	74-75
>>END_MODULE