FastQCFastQC Report
Fri 27 May 2016
SRR2139980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23555166
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267450.046.7610051
ATCTCGT176800.043.5658594
TATCTCG218800.034.53890694
GTATCAA457750.033.7528571
TCAACGC551550.027.2498574
ATCAACG552350.027.2180613
CGCTATC329400.026.7502594
CAACGCA563750.026.6451175
AACGCAG579600.025.9968856
GCCGTCT106450.025.8796790-91
TATCAAC591600.025.6501942
ATGCCGT104300.025.26369388-89
TATGCCG98400.025.11780786-87
TGCCGTC105000.024.04012988-89
CCGTCTT108300.023.91075790-91
TCGTATG117100.022.70961484-85
ACGCAGA669500.022.4807247
CGCAGAG673200.022.3382848
CTATCTC361400.021.90540194
TCTCGTA117450.021.85330482-83
Produced by FastQC (version 0.11.5)