##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23555166
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.437301694244056	31.0	31.0	34.0	30.0	34.0
2	31.605498513574474	31.0	31.0	34.0	30.0	34.0
3	31.65666576070829	31.0	31.0	34.0	30.0	34.0
4	35.445926129325514	37.0	35.0	37.0	33.0	37.0
5	35.260101329788974	37.0	35.0	37.0	33.0	37.0
6	35.253656756229184	37.0	35.0	37.0	32.0	37.0
7	35.162059651797826	37.0	35.0	37.0	32.0	37.0
8	35.09635979640305	37.0	35.0	37.0	32.0	37.0
9	36.64723398680358	39.0	35.0	39.0	32.0	39.0
10-11	36.57918515624131	39.0	35.0	39.0	32.0	39.0
12-13	36.533818016820604	39.0	35.0	39.0	32.0	39.0
14-15	37.621631237920376	39.0	36.0	41.0	32.0	41.0
16-17	37.586161396612525	39.0	36.0	41.0	32.0	41.0
18-19	37.59572874587256	39.0	36.0	41.0	32.0	41.0
20-21	37.567014526480676	39.0	36.0	41.0	32.0	41.0
22-23	37.489813438837544	39.0	36.0	41.0	32.0	41.0
24-25	37.369047259142974	39.0	36.0	41.0	31.5	41.0
26-27	37.134560060076666	39.0	36.0	40.5	31.0	41.0
28-29	36.9725139982708	39.0	36.0	40.0	31.0	41.0
30-31	36.800383077823284	39.0	35.5	40.0	30.0	41.0
32-33	36.57634103776942	38.0	35.0	40.0	30.0	41.0
34-35	36.41696157682954	38.0	35.0	40.0	30.0	41.0
36-37	36.25725697258714	38.0	35.0	40.0	30.0	41.0
38-39	36.086862350243564	38.0	34.5	40.0	29.5	41.0
40-41	35.92517153091539	38.0	34.0	40.0	29.0	41.0
42-43	35.707333473723324	38.0	34.0	40.0	28.5	41.0
44-45	36.14625034686529	38.0	34.5	40.0	29.5	41.0
46-47	36.410322944123614	38.0	35.0	40.0	30.0	41.0
48-49	36.46270299144092	38.0	35.0	40.0	30.0	41.0
50-51	36.38889074268394	38.0	35.0	40.0	30.0	41.0
52-53	36.255304856343955	38.0	34.5	40.0	29.0	41.0
54-55	36.13280193952154	38.0	34.0	40.0	29.0	41.0
56-57	36.014084406150246	38.0	34.0	40.0	28.5	41.0
58-59	35.84598575066185	38.0	34.0	40.0	28.0	41.0
60-61	35.66677507946878	38.0	34.0	40.0	28.0	41.0
62-63	35.45714287540125	37.0	34.0	40.0	28.0	41.0
64-65	35.21498591181289	37.0	33.0	40.0	28.0	41.0
66-67	34.91812771563137	36.5	33.0	39.0	27.5	41.0
68-69	34.64793634895463	36.0	32.5	39.0	27.0	40.5
70-71	34.365105300921	36.0	32.0	39.0	26.5	40.0
72-73	34.05743059790852	35.0	32.0	38.5	26.0	40.0
74-75	33.71312533991467	35.0	32.0	37.5	26.0	40.0
76-77	32.00236922732844	33.5	29.5	36.0	25.0	38.5
78-79	32.99467837480465	34.0	31.0	36.0	26.0	39.0
80-81	33.036582620252936	34.0	31.0	36.0	26.0	39.0
82-83	32.79545393985272	34.0	31.0	36.0	26.0	37.5
84-85	32.561350422716274	34.0	31.0	35.0	26.0	37.0
86-87	32.343603306935954	34.0	31.0	35.0	26.0	37.0
88-89	32.13346067230969	34.0	31.0	35.0	26.0	36.0
90-91	31.99435215325782	34.0	31.0	35.0	26.0	36.0
92-93	31.966633718688897	34.0	31.0	35.0	26.0	35.5
94-95	31.932486437345464	34.0	31.0	35.0	26.0	35.0
96-97	32.032679270050444	34.0	31.0	35.0	26.0	35.0
98-99	32.24640389507834	34.0	31.0	35.0	27.0	35.0
100	30.373173426119077	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	43.0
20	290.0
21	2207.0
22	10177.0
23	33849.0
24	84577.0
25	170222.0
26	292380.0
27	438232.0
28	598559.0
29	762439.0
30	923748.0
31	1096615.0
32	1294494.0
33	1549603.0
34	1892258.0
35	2353190.0
36	3068658.0
37	3946006.0
38	3661271.0
39	1336178.0
40	40165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.508242056116266	21.87597404323111	14.95643885506899	23.65934504558363
2	14.440666964301197	22.565337353326303	41.17089719129796	21.823098491074536
3	17.540712725183088	27.415667544011363	31.640706756216446	23.4029129745891
4	12.303469545994401	19.35330175238275	39.807501788994735	28.535726912628114
5	12.366548382635045	38.68106469723032	36.57404070088065	12.378346219253984
6	28.522851420363583	39.867114500487915	19.137216014525222	12.472818064623276
7	24.962112345122083	33.42850990733837	24.462680500744508	17.146697246795036
8	23.518484964473153	37.644278464186	21.851231306418676	16.986005264922177
9	25.082022347029948	16.54199762379089	21.792234450820683	36.58374557835848
10-11	23.271048405161782	27.296980978289536	30.144469173393766	19.287501443154916
12-13	24.119311189038896	24.996022099972905	29.982464126091873	20.902202584896326
14-15	21.378840280134842	26.45713653760961	27.41462618368815	24.7493969985674
16-17	21.00144279254333	29.46418416630174	28.10516146986109	21.42921157129384
18-19	20.977298330134563	28.71434780865332	29.698524885936354	20.60982897527576
20-21	21.79462015929032	27.95482363054781	29.46515982251034	20.785396387651534
22-23	21.487603095224042	27.895598397100958	29.33370007341879	21.283098434256214
24-25	21.332710334683576	28.00086249588059	29.379437102496404	21.286990066939435
26-27	21.35636119765691	28.155575039280293	29.223291418769797	21.264772344292997
28-29	21.291471682166545	28.224501611685675	29.267507158688037	21.21651954745974
30-31	21.565445736706067	28.0257085302548	29.205392156789678	21.203453576249455
32-33	21.395819405436846	28.203455331654816	29.08487862488684	21.315846638021497
34-35	21.490565665203295	28.241435385480727	29.069525756127806	21.198473193188168
36-37	21.48022563367129	28.153394818214128	29.0773583923288	21.28902115578578
38-39	21.337602439548522	28.223175948604634	29.078649891054702	21.36057172079214
40-41	21.634839449744003	28.104250056250883	29.003093651914526	21.257816842090588
42-43	21.4624759907171	28.199122911370857	28.786072246938293	21.552328850973755
44-45	21.537520803926473	28.31791067741364	28.592496707997345	21.552071810662536
46-47	21.601551811412893	28.266484797075307	28.451343263386313	21.68062012812548
48-49	21.523485189836563	28.427140416654435	28.421448636939957	21.627925756569045
50-51	21.47255231793994	28.528970081303356	28.244129861515056	21.75434773924165
52-53	21.56904941515185	28.36354908600444	28.130012080580517	21.937389418263194
54-55	21.672534320891643	28.124597821824043	28.405897603903618	21.796970253380696
56-57	21.527353399692586	27.900668803647218	28.500171382872804	22.071806413787392
58-59	21.39062921477089	27.75984897918385	28.551244448088493	22.29827735795677
60-61	21.527163220584725	27.95926970653955	28.772008059119873	21.74155901375585
62-63	21.13959753877302	28.15084023251586	28.74732124166276	21.962240987048354
64-65	20.9843668921435	28.42735639099554	28.47552360307387	22.112753113787086
66-67	21.12074225112462	28.93086661008405	28.01845645439946	21.92993468439187
68-69	21.200246460319846	28.958346767611136	27.82146592225715	22.019940849811864
70-71	21.149211897289444	28.454231674278883	27.819049830318583	22.577506598113093
72-73	21.325812137970697	28.038135267547563	27.89343019232127	22.74262240216047
74-75	21.457192239623353	28.03956671980432	27.99390278454107	22.509338256031256
76-77	21.69708160742135	27.97351460618287	27.508113927535643	22.82128985886013
78-79	21.915682381772797	28.02002127886185	27.142073459945195	22.922222879420158
80-81	21.76473550056669	28.27445220972234	27.288184193753835	22.672628095957133
82-83	21.711143723534644	28.05675117678274	27.406754498168674	22.825350601513943
84-85	21.800131078481762	28.410058551015773	27.34699689021704	22.44281348028543
86-87	22.128262033297165	28.272426631430392	27.459480855680045	22.139830479592394
88-89	21.978001277031865	28.3724323044885	27.723497177968515	21.926069240511122
90-91	21.889813927551675	28.0189369562427	27.764395091425286	22.32685402478034
92-93	21.818968357595566	27.58502701081515	28.00081503215845	22.59518959943084
94-95	21.154209026909633	27.967806455108814	28.6096957063605	22.268288811621055
96-97	21.242657612994346	27.878001216006503	28.817761812462873	22.061579358536278
98-99	22.291578530091563	29.81809382129948	29.31527539066033	18.575052257948627
100	21.94060261131365	30.455778120090542	22.88089107871081	24.722728189884993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3931.0
1	3671.0
2	5634.0
3	8298.5
4	11306.0
5	18908.0
6	25661.5
7	30157.0
8	32685.0
9	31782.5
10	28913.5
11	25988.5
12	27829.0
13	36511.0
14	56831.5
15	88681.5
16	115699.0
17	130288.5
18	136769.5
19	135933.5
20	130526.0
21	125047.0
22	121439.5
23	127267.5
24	148630.0
25	182344.0
26	228065.0
27	280489.5
28	336028.5
29	399139.0
30	459453.0
31	518847.0
32	581651.5
33	637897.0
34	695881.5
35	749355.5
36	800529.5
37	846758.5
38	874842.5
39	896942.5
40	924254.0
41	948011.0
42	960999.0
43	972041.5
44	980689.0
45	987417.5
46	985452.0
47	974990.0
48	960614.5
49	933382.5
50	899292.5
51	856271.0
52	801738.5
53	743206.0
54	683301.5
55	620121.5
56	555647.0
57	491811.0
58	430374.0
59	371824.0
60	310372.0
61	250651.5
62	197625.0
63	153117.0
64	116082.0
65	87420.5
66	66558.5
67	52025.5
68	41290.0
69	31426.5
70	23355.0
71	17626.5
72	13387.0
73	10079.0
74	7632.5
75	5812.5
76	4416.0
77	3245.0
78	2335.5
79	1740.5
80	1288.0
81	911.0
82	691.0
83	509.0
84	366.0
85	269.5
86	198.0
87	144.5
88	109.5
89	82.0
90	57.0
91	41.5
92	32.5
93	20.0
94	9.5
95	4.5
96	2.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.11645003902753222
3	0.0
4	1.6132342264113105E-4
5	0.0
6	0.0
7	0.0
8	9.339777100276008E-5
9	0.0
10-11	1.0613383068495462E-5
12-13	1.0188847745755645E-4
14-15	3.43873611419253E-4
16-17	6.41048337337126E-4
18-19	1.910408952329183E-5
20-21	7.217576037375835E-5
22-23	0.0
24-25	0.0064882632839120226
26-27	3.1874721295405674E-5
28-29	0.0022243145049717894
30-31	0.0020740567295400424
32-33	0.02364892445950842
34-35	0.016036934022554652
36-37	0.008423143641930766
38-39	0.004419965411502345
40-41	8.795061006452057E-4
42-43	2.951649876078806E-4
44-45	0.0010539942279924463
46-47	0.001844923025538453
48-49	0.0013264184513559598
50-51	5.202847604454008E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	6.628768397689531E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.2507241873437633E-4
70-71	5.771070824870111E-4
72-73	9.863171914716605E-6
74-75	5.2444196752445555E-5
76-77	3.11219209603405E-4
78-79	1.4607541114910582E-4
80-81	2.599429544788678E-5
82-83	2.639162372089697E-6
84-85	0.0
86-87	1.4722959703150233E-4
88-89	5.084540346716751E-4
90-91	5.678315532240014E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	955.0
20-21	3148.0
22-23	5929.0
24-25	11874.0
26-27	14808.0
28-29	25773.0
30-31	51373.0
32-33	67531.0
34-35	94923.0
36-37	121460.0
38-39	95559.0
40-41	151882.0
42-43	179094.0
44-45	214337.0
46-47	193465.0
48-49	178084.0
50-51	107383.0
52-53	47342.0
54-55	49546.0
56-57	52694.0
58-59	131070.0
60-61	214850.0
62-63	213853.0
64-65	229489.0
66-67	248483.0
68-69	252611.0
70-71	256639.0
72-73	254518.0
74-75	266156.0
76-77	243683.0
78-79	270189.0
80-81	288490.0
82-83	292112.0
84-85	306020.0
86-87	333327.0
88-89	373088.0
90-91	414798.0
92-93	478574.0
94-95	591174.0
96-97	1175199.0
98-99	1989607.0
100-101	1.3064076E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.74355369556162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07280675037912	71.99384207718438
2	7.82624597708212	12.794903165245708
3	2.1274529166302387	5.217166851760292
4	0.864110230390926	2.825417640673791
5	0.41490543699903915	1.6957922433955712
6	0.2352201286703285	1.1536637530943945
7	0.1369081527680354	0.7833951256007838
8	0.08946237882361299	0.5850378213680547
9	0.054575746461265284	0.40150939151986165
>10	0.17475454378992142	2.1911533772651075
>50	0.0026344250588502737	0.14134685114751705
>100	8.154495491124652E-4	0.1055097495750191
>500	4.923275511762657E-5	0.02530757240146241
>1k	5.863064237494175E-5	0.08595437976801544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2736059682194555E-5	0.0	4.245353227398185E-6	0.0
3	0.0	1.2736059682194555E-5	0.0	1.698141290959274E-5	0.0
4	0.0	3.820817904658367E-5	0.0	2.9717472591787295E-5	0.0
5	0.0	4.245353227398185E-5	0.0	3.396282581918548E-5	0.0
6	0.0	4.245353227398185E-5	0.0	3.820817904658367E-5	8.49070645479637E-6
7	0.0	4.245353227398185E-5	0.0	3.820817904658367E-5	8.49070645479637E-6
8	0.0	6.368029841097277E-5	0.0	3.820817904658367E-5	1.2736059682194555E-5
9	0.0	1.1037918391235282E-4	0.0	7.641635809316734E-5	2.1226766136990925E-5
10-11	0.0	1.2311524359454737E-4	0.0	9.552044761645916E-5	2.1226766136990925E-5
12-13	0.0	1.2736059682194554E-4	0.0	1.0825650729865373E-4	2.3349442750690016E-5
14-15	0.0	1.8679554200552015E-4	0.0	1.3372862666304283E-4	2.9717472591787295E-5
16-17	0.0	1.995316016877147E-4	0.0	1.6556877586852922E-4	2.9717472591787295E-5
18-19	0.0	2.2288104443840472E-4	0.0	1.974089250740156E-4	2.9717472591787295E-5
20-21	0.0	2.4623048718909474E-4	0.0	2.589665468712893E-4	2.9717472591787295E-5
22-23	0.0	2.801933130082802E-4	0.0	4.967063276055877E-4	3.820817904658367E-5
24-25	0.0	3.3113755173705845E-4	0.0	0.0012014349633536862	4.245353227398185E-5
26-27	0.0	3.523643178740494E-4	0.0	0.0028783494881759694	4.245353227398185E-5
28-29	0.0	3.651003775562439E-4	0.0	0.010415974143421448	4.4576208887680944E-5
30-31	0.0	4.2028996951242034E-4	0.0	0.014207074575488025	4.669888550138004E-5
32-33	0.0	4.3302602919461486E-4	0.0	0.022960992930383086	5.943494518357459E-5
34-35	0.0	5.009516808329858E-4	0.0	0.03373994477474708	6.792565163837096E-5
36-37	0.0	5.540185961754633E-4	0.0	0.05405820532107479	6.792565163837096E-5
38-39	0.0	5.794907155398523E-4	0.0	0.1841528096214648	7.004832825207005E-5
40-41	0.0	6.346803074960287E-4	0.0	0.32087228763320963	7.641635809316734E-5
42-43	0.0	6.559070736330197E-4	0.0	0.3646864556165726	7.641635809316734E-5
44-45	0.0	7.195873720439924E-4	0.0	0.4285981257784386	7.641635809316734E-5
46-47	0.0	7.662862575453724E-4	0.0	0.49451572534025023	8.278438793426461E-5
48-49	0.0	7.960037301371597E-4	0.0	0.5554789976856882	8.915241777536189E-5
50-51	0.0	8.787881180714243E-4	0.0	0.6162045302503918	8.915241777536189E-5
52-53	0.0	8.978922075947161E-4	0.0	1.0466769794787267	9.764312423015827E-5
54-55	0.0	9.106282672769107E-4	0.0	1.6279847061999053	1.0825650729865373E-4
56-57	0.0	9.38223063254999E-4	0.0	2.2735543447242104	1.1037918391235282E-4
58-59	0.0	9.679405358467862E-4	0.0	2.9477164372350426	1.1037918391235282E-4
60-61	0.0	0.0010019033616659716	0.0	3.134210559161417	1.14624537139751E-4
62-63	0.0	0.0010252528044166617	0.0	3.1430939607897477	1.14624537139751E-4
64-65	0.0	0.001101669162509829	0.0	3.1506995110966316	1.14624537139751E-4
66-67	0.0	0.001125018605260519	2.1226766136990925E-6	3.1562248383220903	1.2311524359454737E-4
68-69	0.0	0.0011632267843071026	4.245353227398185E-6	3.1616503997467054	1.3160595004934374E-4
70-71	0.0	0.0011695948141482	4.245353227398185E-6	3.1665240652517586	1.3585130327674192E-4
72-73	0.0	0.0011780855206029964	4.245353227398185E-6	3.1706420578823344	1.698141290959274E-4
74-75	0.0	0.0012226617294906774	4.245353227398185E-6	3.174053199200549	1.719368057096265E-4
76-77	0.0	0.001284219351287951	4.245353227398185E-6	3.177468585871991	1.740594823233256E-4
78-79	0.0	0.0013033234408112429	4.245353227398185E-6	3.1796464520776464	1.740594823233256E-4
80-81	0.0	0.00130969147065234	4.245353227398185E-6	3.1804297197481013	1.9316357184661742E-4
82-83	0.0	0.0013203048537208356	4.245353227398185E-6	3.1810007197571863	2.1651301459730743E-4
84-85	0.0	0.0013521450029263219	4.245353227398185E-6	3.1813085078661727	2.1863569121100652E-4
86-87	0.0	0.001375494445677012	4.245353227398185E-6	3.1814889353783373	2.2075836782470563E-4
88	0.0	0.001422193331178392	4.245353227398185E-6	3.1815016714380193	2.250037210521038E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26745	0.0	46.761005	1
ATCTCGT	17680	0.0	43.56585	94
TATCTCG	21880	0.0	34.538906	94
GTATCAA	45775	0.0	33.752857	1
TCAACGC	55155	0.0	27.249857	4
ATCAACG	55235	0.0	27.218061	3
CGCTATC	32940	0.0	26.75025	94
CAACGCA	56375	0.0	26.645117	5
AACGCAG	57960	0.0	25.996885	6
GCCGTCT	10645	0.0	25.87967	90-91
TATCAAC	59160	0.0	25.650194	2
ATGCCGT	10430	0.0	25.263693	88-89
TATGCCG	9840	0.0	25.117807	86-87
TGCCGTC	10500	0.0	24.040129	88-89
CCGTCTT	10830	0.0	23.910757	90-91
TCGTATG	11710	0.0	22.709614	84-85
ACGCAGA	66950	0.0	22.480724	7
CGCAGAG	67320	0.0	22.338284	8
CTATCTC	36140	0.0	21.905401	94
TCTCGTA	11745	0.0	21.853304	82-83
>>END_MODULE