FastQCFastQC Report
Fri 27 May 2016
SRR2139979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9845447
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106000.046.1432041
GTATCAA186400.034.2826961
ATCAACG215750.028.401453
TCAACGC226650.027.0542584
CAACGCA230600.026.6275675
AACGCAG234500.026.2208396
ACGCAGA236050.025.994847
TATCAAC242550.025.7177562
CGCAGAG273350.022.4632058
GTACATG255550.018.85811
ACATGGG244550.018.5803933
GCAGAGT328650.018.5030619
TACATGG253350.018.4702592
AGAGTAC299000.018.33384910-11
GAGTACT194300.017.2504712-13
CAGAGTA323450.016.87596310-11
AGTACTT218200.015.96261212-13
GTGGTAT54300.015.5977431
CATGGGG176050.015.4907574
GTACTTT222900.015.11495814-15
Produced by FastQC (version 0.11.5)