##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9845447
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12926228743093	33.0	31.0	34.0	30.0	34.0
2	32.27616653667426	33.0	31.0	34.0	30.0	34.0
3	33.019210910383244	34.0	31.0	34.0	30.0	37.0
4	35.842863203671705	37.0	35.0	37.0	35.0	37.0
5	35.7518373721376	37.0	35.0	37.0	33.0	37.0
6	35.75460748506391	37.0	35.0	37.0	33.0	37.0
7	35.72067809617989	37.0	35.0	37.0	33.0	37.0
8	35.98482780924015	37.0	35.0	37.0	33.0	39.0
9	37.206342586578344	39.0	37.0	39.0	33.0	39.0
10-11	37.14649019998787	39.0	37.0	39.0	33.0	39.0
12-13	37.267926433406224	39.0	37.0	39.0	33.0	39.5
14-15	38.291230657175845	40.0	38.0	41.0	33.0	41.0
16-17	38.28659963331274	40.0	38.0	41.0	33.0	41.0
18-19	38.32511449200834	40.0	38.0	41.0	33.5	41.0
20-21	38.293126557589325	40.0	38.0	41.0	33.0	41.0
22-23	38.214599499045036	40.0	38.0	41.0	33.0	41.0
24-25	38.12590243232911	40.0	37.0	41.0	33.0	41.0
26-27	37.96526025434992	40.0	37.0	41.0	33.0	41.0
28-29	37.867749454881945	40.0	37.0	41.0	33.0	41.0
30-31	37.771571741733574	40.0	37.0	41.0	33.0	41.0
32-33	37.9271595037581	40.0	37.0	41.0	33.0	41.0
34-35	37.90432196733252	40.0	37.0	41.0	33.0	41.0
36-37	37.80652127955703	40.0	37.0	41.0	33.0	41.0
38-39	37.65507292227454	40.0	37.0	41.0	32.0	41.0
40-41	37.67231924667306	40.0	37.0	41.0	32.5	41.0
42-43	37.64127107048773	40.0	37.0	41.0	32.0	41.0
44-45	37.6363201672594	40.0	37.0	41.0	32.0	41.0
46-47	37.648138734606434	40.0	37.0	41.0	32.0	41.0
48-49	37.573191627528146	40.0	37.0	41.0	32.0	41.0
50-51	37.48182566342615	40.0	36.0	41.0	32.0	41.0
52-53	37.35882461547732	39.0	36.0	41.0	31.0	41.0
54-55	37.25629875126728	39.0	36.0	41.0	31.0	41.0
56-57	37.113571174852666	39.0	35.5	41.0	31.0	41.0
58-59	36.97135636219757	39.0	35.0	40.0	31.0	41.0
60-61	36.775242465866235	38.5	35.0	40.0	31.0	41.0
62-63	36.55634494268108	38.0	35.0	40.0	31.0	41.0
64-65	36.35346782905293	38.0	35.0	40.0	30.0	41.0
66-67	36.07778330255094	37.5	34.0	40.0	30.0	41.0
68-69	35.80259372611461	37.0	34.0	39.5	30.0	41.0
70-71	35.5212065333143	36.5	34.0	39.0	29.0	41.0
72-73	35.21578138389846	36.0	34.0	39.0	29.0	40.0
74-75	34.76909587950662	35.5	33.5	38.5	29.0	40.0
76-77	33.92950595883218	35.0	32.5	37.0	28.0	39.0
78-79	34.19013408416977	35.0	33.0	37.0	29.0	39.0
80-81	34.061204096083046	35.0	33.0	37.0	29.0	39.0
82-83	33.836509834688584	35.0	33.0	36.0	29.0	38.5
84-85	33.61079467678354	35.0	33.0	36.0	29.0	37.0
86-87	33.39387037828213	35.0	33.0	35.5	29.0	37.0
88-89	33.18831758714693	35.0	33.0	35.0	29.0	36.5
90-91	33.08833131231596	35.0	33.0	35.0	29.0	36.0
92-93	33.01959138951961	34.0	33.0	35.0	29.0	36.0
94-95	32.966311306057044	34.0	32.5	35.0	29.0	35.5
96-97	32.91576981187872	34.0	32.0	35.0	29.0	35.0
98-99	33.02607078925536	34.0	32.0	35.0	29.0	35.0
100-101	32.46010951031121	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	3.0
19	12.0
20	69.0
21	452.0
22	2113.0
23	7081.0
24	18056.0
25	37616.0
26	66986.0
27	103193.0
28	145191.0
29	189769.0
30	238850.0
31	293591.0
32	360653.0
33	451589.0
34	592872.0
35	800745.0
36	1197981.0
37	1939784.0
38	2302439.0
39	1043251.0
40	53148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.732176935773985	0.010827388172937156	27.530889289143367	30.726106386909706
2	13.976446713087975	26.398532909240775	37.59379442141888	22.031225956252378
3	15.36264036062166	25.603906294516154	34.711358341112735	24.32209500374945
4	12.502987421495437	25.376511599727262	37.72822097361349	24.39228000516381
5	16.551955436863356	37.40160299476499	32.93183133279779	13.114610235573865
6	26.780805381411326	38.76101308554097	20.88672053183568	13.571461001212032
7	24.77422304949689	33.40223150863541	23.592966373187522	18.23057906868017
8	23.46667449431194	32.78465670476922	22.711340582098508	21.037328218820335
9	22.879773767508983	20.605930842957157	24.702768701106205	31.811526688427655
10-11	23.974503138354205	26.516099269032683	29.69333946950301	19.816058123110103
12-13	24.05641409679012	24.701438136836245	29.344630060981487	21.897517705392147
14-15	21.6150318009939	27.299283618102866	27.07963386527803	24.0060507156252
16-17	21.008702906907416	29.402187569765758	28.13226033640325	21.45684918692358
18-19	21.066164179496354	28.639209143373236	29.507180771415108	20.7874459057153
20-21	21.80992739428539	28.037440468895674	29.25383010038296	20.898802036435974
22-23	21.570077568457037	27.860161614158592	29.110886443826157	21.458874373558213
24-25	21.414132268771645	28.07720480511954	29.206205783724908	21.302457142383908
26-27	21.394582469310073	28.31442711921958	29.009059885929865	21.28193052554048
28-29	21.363770639970348	28.143064908430915	29.078906464816274	21.414257986782463
30-31	21.620501452757185	28.09062227213423	29.075612213017827	21.213264062090754
32-33	21.484682252717562	28.30705737060693	28.93887754777661	21.2693828288989
34-35	21.585318145937872	28.203230426323582	28.931787735663654	21.279663692074887
36-37	21.522619309683645	28.180065711105673	28.993970995768077	21.303343983442613
38-39	21.39082791149642	28.290105901190614	29.013130150158776	21.30593603715419
40-41	21.6250662485468	28.0349537299799	28.85515276681985	21.484827254653453
42-43	21.503372428001974	28.110383479648192	28.84551813443404	21.54072595791579
44-45	21.59167775686665	28.465803929842497	28.48348978998147	21.459028523309385
46-47	21.660138869241123	28.196005727021806	28.311693657762188	21.832161745974876
48-49	21.577497483730003	28.316640259620414	28.432621405823433	21.673240850826154
50-51	21.562971051211843	28.506547411743675	28.177030657039825	21.753450880004653
52-53	21.726878555024058	28.278081784681003	28.169255103544224	21.825784556750715
54-55	21.65417558591453	28.250527769243888	28.293397480266318	21.801899164575268
56-57	21.718142537729115	28.48116005746604	28.032129478609825	21.768567926195022
58-59	21.726871655488793	28.314865128863374	28.189811870841403	21.768451344806433
60-61	21.74565563908863	28.4038264375354	28.117539810008186	21.732978113367782
62-63	21.660458898749845	28.55027765793259	28.068798377505537	21.720465065812025
64-65	21.673781661786045	28.363402087165362	28.048105615941843	21.91471063510675
66-67	21.698100536683995	28.462179381766944	27.943931051429725	21.895789030119335
68-69	21.73209375483444	28.48349397491438	27.95675049350821	21.827661776742964
70-71	21.755105326290725	28.120517210892395	28.014787254392083	22.1095902084248
72-73	21.72062472984696	28.215777844276985	28.016624802391554	22.0469726234845
74-75	21.625495186212035	28.357483313363808	27.967652421328314	22.049369079095847
76-77	21.89811198132293	28.20802433783894	27.793223210414876	22.100640470423258
78-79	21.75286846107239	28.266128385074975	27.9091005300559	22.071902623796742
80-81	21.813888132756496	28.28888408269037	27.834920766470166	22.062307018082965
82-83	21.88297614939835	28.135500825906295	27.941780935563198	22.039742089132154
84-85	21.928387259277223	28.188041776493876	27.950617732330272	21.93295323189863
86-87	21.8233774736139	28.244960003392688	27.92011678267185	22.011545740321566
88-89	21.911351935517764	28.114296029818814	27.892217833174005	22.08213420148942
90-91	21.90204736855577	28.100423265460932	28.038349441273986	21.95917992470931
92-93	21.862673579530973	28.13868249012875	28.01603662182898	21.982607308511287
94-95	21.84188434386631	28.026075768560716	28.08217251840232	22.049867369170652
96-97	21.676943231434652	28.200623264555148	28.25021760220421	21.872215901805994
98-99	22.03800200174181	29.151290078754457	28.186323358722515	20.62438456078122
100-101	22.468939471391376	31.232557970033838	25.08564532876164	21.212857229813142
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1521.0
1	1412.0
2	2175.5
3	3233.0
4	4313.0
5	6698.0
6	8665.0
7	10144.5
8	11170.0
9	11021.0
10	10360.0
11	9796.5
12	11020.0
13	14523.0
14	22784.0
15	35530.0
16	46487.0
17	52074.5
18	52853.5
19	51209.5
20	48719.0
21	46560.5
22	46817.0
23	51433.5
24	61642.5
25	76998.5
26	97150.5
27	120852.5
28	147014.5
29	174561.5
30	201271.5
31	227157.5
32	253055.5
33	277928.5
34	301996.5
35	322889.0
36	344418.5
37	363885.0
38	375098.5
39	383061.5
40	391291.0
41	398004.5
42	402111.5
43	406384.0
44	409326.0
45	410088.5
46	408651.0
47	405578.5
48	401953.5
49	392097.0
50	377522.5
51	361262.5
52	340359.0
53	315748.0
54	289849.0
55	263761.5
56	237608.0
57	211873.5
58	187085.5
59	161665.5
60	134522.0
61	108392.0
62	84978.0
63	65682.5
64	49248.0
65	36903.0
66	28383.5
67	21928.5
68	16994.0
69	12694.5
70	9424.5
71	6992.0
72	5192.0
73	3967.0
74	3004.5
75	2225.5
76	1615.0
77	1146.0
78	818.0
79	588.5
80	414.0
81	300.5
82	226.5
83	162.5
84	111.5
85	74.0
86	49.5
87	40.0
88	36.0
89	28.5
90	20.0
91	14.0
92	9.0
93	4.0
94	2.5
95	3.0
96	2.5
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.4690708304051606E-4
2	0.0011375816659213137
3	4.0627916640046916E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.0156979160011729E-5
18-19	2.1837505194025218E-4
20-21	0.002234644021461318
22-23	6.501583338717458E-4
24-25	0.002956915455537975
26-27	0.01007879657034258
28-29	3.966556638792791E-4
30-31	0.002790160955628091
32-33	0.003107391448458734
34-35	0.004344808728879381
36-37	0.06170236432521854
38-39	0.026603755473836466
40-41	0.02299921287535483
42-43	0.002907926252475274
44-45	0.0016745292063777483
46-47	0.003556466883161478
48-49	0.002238427986462461
50-51	7.542731609477296E-4
52-53	0.0010609654829646133
54-55	0.0022266620737168446
56-57	0.01276316594778431
58-59	0.007938279567805278
60-61	0.004140700434314726
62-63	0.002482321041096884
64-65	0.001010888657199627
66-67	4.905252708108267E-4
68-69	0.0013290388249453853
70-71	1.6732232117411985E-4
72-73	4.5944697335074556E-4
74-75	8.195696598736981E-4
76-77	0.0044447192989526246
78-79	9.905848049055767E-4
80-81	7.118151075511317E-4
82-83	8.312153450858161E-4
84-85	0.002998166807158107
86-87	0.01202536656663196
88-89	0.032660953265163405
90-91	3.216604578213828E-4
92-93	6.205113152678552E-4
94-95	0.0021287690247162186
96-97	0.016370114525643353
98-99	1.86555272440656E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	294.0
20-21	994.0
22-23	1930.0
24-25	3648.0
26-27	4721.0
28-29	9227.0
30-31	19474.0
32-33	26309.0
34-35	38457.0
36-37	57625.0
38-39	58229.0
40-41	64116.0
42-43	74068.0
44-45	86848.0
46-47	86922.0
48-49	79960.0
50-51	72953.0
52-53	69407.0
54-55	75463.0
56-57	81301.0
58-59	84823.0
60-61	85891.0
62-63	84846.0
64-65	90947.0
66-67	98094.0
68-69	97456.0
70-71	96914.0
72-73	95267.0
74-75	97882.0
76-77	99935.0
78-79	106869.0
80-81	108500.0
82-83	105028.0
84-85	106748.0
86-87	113512.0
88-89	122622.0
90-91	132351.0
92-93	141865.0
94-95	179323.0
96-97	273818.0
98-99	966195.0
100-101	5644615.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.77443983603585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.907098758061	77.11731033255836
2	7.0610821704772455	12.113207356178119
3	1.8089672593991093	4.6548946007006275
4	0.5874080782160338	2.0153839545657055
5	0.26167517069676766	1.1222520592757155
6	0.14503494322906174	0.746417460727442
7	0.0751344452699208	0.4511230468793125
8	0.039322933948756106	0.26983221057311935
9	0.025629872716334687	0.19785491777812558
>10	0.08672065377591877	1.1202304993278762
>50	0.0015363722065971747	0.09271431299894518
>100	3.1686252730251383E-4	0.052122829809013664
>500	5.4361421229442504E-5	0.030758323488235765
>1k	1.8118054737573708E-5	0.01589809513942803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.10988541200821E-5	0.0	1.0156979160011729E-5	0.0
3	0.0	7.10988541200821E-5	0.0	1.0156979160011729E-5	0.0
4	0.0	7.10988541200821E-5	0.0	2.0313958320023458E-5	0.0
5	0.0	7.10988541200821E-5	0.0	6.094187496007038E-5	0.0
6	0.0	8.125583328009383E-5	0.0	6.094187496007038E-5	0.0
7	0.0	8.125583328009383E-5	0.0	7.10988541200821E-5	0.0
8	0.0	1.2188374992014075E-4	0.0	7.10988541200821E-5	0.0
9	0.0	2.5392447900029325E-4	0.0	8.125583328009383E-5	0.0
10-11	0.0	2.5392447900029325E-4	0.0	1.421977082401642E-4	0.0
12-13	0.0	2.5392447900029325E-4	0.0	1.4727619782017008E-4	0.0
14-15	0.0	3.2502333312037533E-4	0.0	1.625116665601877E-4	0.0
16-17	0.0	3.6565124976042224E-4	0.0	1.8282562488021112E-4	0.0
18-19	0.0	3.6565124976042224E-4	0.0	3.859652080804457E-4	0.0
20-21	5.0784895800058645E-6	3.707297393404281E-4	0.0	7.160670307808269E-4	0.0
22-23	1.0156979160011729E-5	4.2659312472049263E-4	0.0	0.001010619426421167	1.5235468740017594E-5
24-25	1.0156979160011729E-5	4.51985572620522E-4	0.0	0.0014930759365217243	3.554942706004105E-5
26-27	1.0156979160011729E-5	4.6722104136053954E-4	0.0	0.003722532862144299	4.570640622005278E-5
28-29	1.0156979160011729E-5	4.87534999680563E-4	0.0	0.01501709368807734	5.078489580005864E-5
30-31	1.0156979160011729E-5	5.433983850606275E-4	0.0	0.023818116130227503	5.078489580005864E-5
32-33	1.0156979160011729E-5	5.586338538006451E-4	0.0	0.03384813305073908	6.094187496007038E-5
34-35	1.0156979160011729E-5	6.348111975007331E-4	0.0	0.046854144865134104	6.094187496007038E-5
36-37	1.0156979160011729E-5	7.109885412008211E-4	0.0	0.06436477693699433	6.094187496007038E-5
38-39	1.0156979160011729E-5	7.211455203608327E-4	0.0	0.09427200207364886	6.602036454007623E-5
40-41	1.0156979160011729E-5	7.973228640609207E-4	0.0	0.13945024537738104	7.10988541200821E-5
42-43	1.0156979160011729E-5	8.887356765010263E-4	0.0	0.16525405093339085	7.617734370008798E-5
44-45	1.0156979160011729E-5	9.649130202011143E-4	0.0	0.20066635877477174	8.125583328009383E-5
46-47	1.0156979160011729E-5	9.903054681011437E-4	0.0	0.237251797709134	9.649130202011143E-5
48-49	1.0156979160011729E-5	0.001015697916001173	0.0	0.2680122090952295	1.1172677076012903E-4
50-51	1.0156979160011729E-5	0.0010817182805412493	0.0	0.29873199256468497	1.1172677076012903E-4
52-53	1.0156979160011729E-5	0.001091875259701261	0.0	0.34358013404571675	1.3711921866015834E-4
54-55	1.0156979160011729E-5	0.0010969537492812667	0.0	0.4174924713931221	1.6251166656018766E-4
56-57	1.0156979160011729E-5	0.0011071107284412786	0.0	0.4919380501464281	1.7266864572019938E-4
58-59	1.0156979160011729E-5	0.001127424686761302	0.0	0.5498277528689149	1.87904114460217E-4
60-61	1.0156979160011729E-5	0.0011426601555013195	0.0	0.5873781048234783	1.9298260404022286E-4
62-63	1.0156979160011729E-5	0.001162974113821343	0.0	0.6129229074109078	1.9298260404022286E-4
64-65	1.0156979160011729E-5	0.0013000933324815013	0.0	0.6298393562019073	1.9298260404022286E-4
66-67	1.0156979160011729E-5	0.00135087822828156	0.0	0.6403467511429395	1.9298260404022286E-4
68-69	1.0156979160011729E-5	0.0013915061449216069	0.0	0.6503361401468111	1.9298260404022286E-4
70-71	1.0156979160011729E-5	0.0014118201032416304	0.0	0.6595485202449416	2.0313958320023457E-4
72-73	1.0156979160011729E-5	0.001421977082401642	0.0	0.6652516640432882	2.1329656236024631E-4
74-75	1.0156979160011729E-5	0.0014727619782017006	0.0	0.6706247060189344	2.1329656236024631E-4
76-77	1.0156979160011729E-5	0.0015337038531617712	0.0	0.6756574891927203	2.1837505194025218E-4
78-79	1.0156979160011729E-5	0.0015438608323217829	0.0	0.6800605396585854	2.2345354152025805E-4
80-81	1.0156979160011729E-5	0.0015438608323217829	0.0	0.6828384734588485	2.285320311002639E-4
82-83	1.0156979160011729E-5	0.0015590963010618003	0.0	0.6847022791347107	2.3361052068026977E-4
84-85	1.0156979160011729E-5	0.0015692532802218122	0.0	0.6861598056441723	2.3361052068026977E-4
86-87	1.0156979160011729E-5	0.0015895672385418357	0.0	0.6863832591856927	2.3361052068026977E-4
88-89	1.0156979160011729E-5	0.001655587603081912	0.0	0.6864289655919127	2.3868901026027564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10600	0.0	46.143204	1
GTATCAA	18640	0.0	34.282696	1
ATCAACG	21575	0.0	28.40145	3
TCAACGC	22665	0.0	27.054258	4
CAACGCA	23060	0.0	26.627567	5
AACGCAG	23450	0.0	26.220839	6
ACGCAGA	23605	0.0	25.99484	7
TATCAAC	24255	0.0	25.717756	2
CGCAGAG	27335	0.0	22.463205	8
GTACATG	25555	0.0	18.8581	1
ACATGGG	24455	0.0	18.580393	3
GCAGAGT	32865	0.0	18.503061	9
TACATGG	25335	0.0	18.470259	2
AGAGTAC	29900	0.0	18.333849	10-11
GAGTACT	19430	0.0	17.25047	12-13
CAGAGTA	32345	0.0	16.875963	10-11
AGTACTT	21820	0.0	15.962612	12-13
GTGGTAT	5430	0.0	15.597743	1
CATGGGG	17605	0.0	15.490757	4
GTACTTT	22290	0.0	15.114958	14-15
>>END_MODULE