FastQCFastQC Report
Fri 27 May 2016
SRR2139978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27270776
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA355400.050.8555221
GTATCAA607450.032.814971
ATCAACG760250.025.706173
TCAACGC763100.025.6319434
CAACGCA777000.025.1626645
AACGCAG794400.024.663876
TATCAAC805350.024.4672092
ACGCAGA918500.021.31347
CGCAGAG926900.021.1067988
GTGGTAT157900.018.6252881
GTACATG763600.018.2928121
TACATGG753300.018.0846062
GAGTACT667450.018.02443912-13
GCAGAGT1078100.017.8384829
TGGTATC152500.017.7027442
AGAGTAC990950.017.58137510-11
ACATGGG759250.017.5741483
CAGAGTA1064850.017.30954710-11
AGTACTT732000.015.81903112-13
GTACTTT765650.015.686748514-15
Produced by FastQC (version 0.11.5)