##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27270776
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189448551079	31.0	31.0	34.0	30.0	34.0
2	31.36472038786135	31.0	31.0	34.0	30.0	34.0
3	31.402339046017612	31.0	31.0	34.0	28.0	34.0
4	35.22934305206423	37.0	35.0	37.0	33.0	37.0
5	35.03930801235726	37.0	35.0	37.0	32.0	37.0
6	35.04781301419512	37.0	35.0	37.0	32.0	37.0
7	34.95300159408738	37.0	35.0	37.0	32.0	37.0
8	34.88874999376622	36.0	35.0	37.0	32.0	37.0
9	36.40391652221411	38.0	35.0	39.0	32.0	39.0
10-11	36.31733396585415	38.0	35.0	39.0	32.0	39.0
12-13	36.26760210270511	38.0	35.0	39.0	32.0	39.0
14-15	37.283274979047164	39.0	36.0	41.0	32.0	41.0
16-17	37.238496513630565	39.0	36.0	41.0	31.5	41.0
18-19	37.24842960097652	39.0	36.0	40.0	31.0	41.0
20-21	37.21904165562633	39.0	36.0	40.0	31.0	41.0
22-23	37.127253236594555	39.0	36.0	40.0	31.0	41.0
24-25	36.99292805429507	39.0	36.0	40.0	31.0	41.0
26-27	36.7257583446779	39.0	35.5	40.0	30.0	41.0
28-29	36.54194131587745	38.0	35.0	40.0	30.0	41.0
30-31	36.3470599581357	38.0	35.0	40.0	30.0	41.0
32-33	36.096430586923546	38.0	34.5	40.0	29.5	41.0
34-35	35.9178500647166	38.0	34.0	40.0	29.0	41.0
36-37	35.7383530504921	38.0	34.0	40.0	29.0	41.0
38-39	35.54693948242807	38.0	34.0	40.0	27.5	41.0
40-41	35.36509687761354	38.0	33.5	40.0	27.0	41.0
42-43	35.12832728642512	38.0	33.0	40.0	27.0	41.0
44-45	35.586287184339724	38.0	33.5	40.0	27.5	41.0
46-47	35.85340937128235	38.0	34.0	40.0	29.0	41.0
48-49	35.89991100080479	38.0	34.0	40.0	28.5	41.0
50-51	35.81589458671475	38.0	34.0	40.0	28.0	41.0
52-53	35.67024651176608	38.0	34.0	40.0	28.0	41.0
54-55	35.52595334824733	38.0	33.5	40.0	28.0	41.0
56-57	35.390660382951694	38.0	33.0	40.0	27.5	41.0
58-59	35.1979997624306	37.0	33.0	40.0	27.0	41.0
60-61	35.007252812359994	37.0	33.0	40.0	27.0	41.0
62-63	34.77767633846162	37.0	32.5	39.5	26.5	41.0
64-65	34.505532949840784	36.0	32.0	39.0	26.0	40.5
66-67	34.19267534913557	36.0	32.0	39.0	26.0	40.0
68-69	33.923533624466394	35.5	31.0	39.0	26.0	40.0
70-71	33.63278106152789	35.0	31.0	38.0	26.0	40.0
72-73	33.321053717019105	35.0	31.0	38.0	26.0	40.0
74-75	32.984421630306414	34.5	31.0	37.0	25.5	39.0
76-77	31.358424996112763	33.0	29.0	35.5	23.5	38.5
78-79	32.40647605422227	34.0	30.0	36.0	25.5	39.0
80-81	32.50232586356512	34.0	31.0	36.0	26.0	38.5
82-83	32.26366730665252	34.0	30.5	35.5	25.5	37.5
84-85	32.01070347958638	34.0	30.0	35.0	25.0	37.0
86-87	31.792394358297493	34.0	30.0	35.0	25.0	36.5
88-89	31.570874163622058	34.0	30.0	35.0	25.0	36.0
90-91	31.416824923928086	34.0	30.0	35.0	25.0	36.0
92-93	31.382353262942388	34.0	30.0	35.0	25.0	35.0
94-95	31.385680854286104	33.5	30.0	35.0	25.0	35.0
96-97	31.529876977613007	33.5	30.0	35.0	25.0	35.0
98-99	31.801614417494108	34.0	30.5	35.0	26.0	35.0
100	29.81408367652497	31.0	27.0	34.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	2.0
18	13.0
19	70.0
20	521.0
21	3486.0
22	15359.0
23	50267.0
24	123563.0
25	244236.0
26	411028.0
27	609146.0
28	822883.0
29	1032448.0
30	1245245.0
31	1458899.0
32	1709637.0
33	2013259.0
34	2410386.0
35	2926797.0
36	3635738.0
37	4190432.0
38	3265269.0
39	1083094.0
40	18997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.628336062017446	22.995810606929556	15.631957081089295	22.743896249963697
2	14.774778802592506	23.55661035966963	40.740465440390814	20.928145397347052
3	17.7772095667538	28.135675347118834	32.0683907197947	22.018724366332663
4	12.621467130792727	20.19909828669191	39.71415969753969	27.46527488497567
5	12.097895564101293	39.073955944634655	36.70108250678308	12.12706598448097
6	27.85176703442542	40.68594527709809	19.14963475920157	12.312652929274913
7	24.404868420319247	33.861199989321904	24.788205513477138	16.945726076881716
8	23.206587512744427	38.164094362208175	21.958942967020928	16.670375158026467
9	24.932678850062793	16.615104755361564	22.068510261680856	36.38370613289479
10-11	23.076931115960665	27.697366700973237	30.241298990681344	18.984403192384754
12-13	23.887748819238606	25.417044803789967	30.20275092534122	20.49245545163021
14-15	21.148486495497252	26.865890262663765	27.716769239581176	24.268854002257807
16-17	20.561447280619223	29.89393428103918	28.445210715535424	21.09940772280617
18-19	20.613713499587405	29.01222941243996	30.136079213456767	20.237977874515867
20-21	21.473478448846254	28.21486414552069	29.97311802572365	20.338539379909403
22-23	21.151607779458107	28.141582312642445	29.859443857182267	20.847366050717188
24-25	20.95938290307251	28.310799979663777	29.909654061613367	20.820163055650344
26-27	20.97263340677914	28.401679581992802	29.777782909839985	20.847904101388075
28-29	20.92698287478273	28.47113626867015	29.791834623910084	20.81004623263704
30-31	21.227110379155203	28.333376882665217	29.667403325083498	20.772109413096082
32-33	21.094801307314114	28.47107590121396	29.54607210265222	20.8880506888197
34-35	21.16215769560571	28.492171758474647	29.58522607248349	20.76044447343615
36-37	21.161674066500723	28.436781050524363	29.524357051347405	20.877187831627513
38-39	21.044050710111307	28.541725563919123	29.549445671946444	20.864778054023123
40-41	21.26953097101192	28.458893461606987	29.423748125811755	20.847827441569336
42-43	21.24185717085864	28.576803266646856	29.131310667109254	21.050028895385246
44-45	21.3036376127363	28.73601171561726	28.891943222414408	21.06840744923203
46-47	21.391605594239426	28.670505087945397	28.642932774028214	21.294956543786963
48-49	21.328162414760733	28.743209628619375	28.69546699786015	21.233160958759733
50-51	21.276962990039593	28.883476535936975	28.53013241659113	21.3094280574323
52-53	21.4555614180078	28.799485655069297	28.43456650760855	21.31038641931436
54-55	21.489739880093328	28.697260724841335	28.497777959664266	21.315221435401067
56-57	21.442161536165276	28.756069275934497	28.464175183704743	21.33759400419548
58-59	21.39086515004249	28.777118234997918	28.507047411099585	21.324969203860007
60-61	21.437165603911893	28.83077267923729	28.471718811104836	21.26034290574598
62-63	21.393425215167237	28.833086614799214	28.436940649194486	21.33654752083906
64-65	21.339770666591427	28.826085361106323	28.431549625312492	21.402594346989755
66-67	21.427845561483892	28.898071491923965	28.25757420305271	21.416508743539424
68-69	21.384339857048353	28.86865766361725	28.31664488509549	21.43035759423891
70-71	21.388447772860104	28.60542887203134	28.42642809546173	21.579695259646826
72-73	21.42414130944663	28.629821363854063	28.404196032835976	21.541841293863328
74-75	21.316818686832484	28.731939018708335	28.340083148161423	21.611159146297755
76-77	21.4096123729532	28.69269065481661	28.336471216809727	21.56122575542046
78-79	21.41096082543936	28.73245145642807	28.27808302435706	21.57850469377551
80-81	21.41498731065214	28.65975140208768	28.307743573422716	21.617517713837465
82-83	21.433188164066493	28.56979841573538	28.468916008074874	21.52809741212325
84-85	21.535099521647748	28.604462353349753	28.393337083454234	21.467101041548265
86-87	21.337606628907107	28.611495873158173	28.481889924743363	21.569007573191357
88-89	21.429901438835476	28.578929447410477	28.47882757507344	21.51234153868061
90-91	21.37370761194006	28.495424687222442	28.680028458519452	21.450839242318043
92-93	21.34728646572575	28.435106280933482	28.691663086215925	21.52594416712484
94-95	21.098999033174294	28.52187170612031	28.839660112326214	21.53946914837918
96-97	21.11406917162587	28.67278397525973	29.000363506710837	21.212783346403562
98-99	22.100080038321952	30.639687116431176	29.35169679899453	17.908536046252344
100	21.912720593588773	30.965706306772596	23.150870480088635	23.970702619549993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6228.0
1	5418.0
2	7766.0
3	11490.0
4	15038.0
5	24444.5
6	33177.0
7	39870.5
8	44512.5
9	44129.5
10	39946.5
11	34840.5
12	36436.0
13	47846.0
14	75625.5
15	120684.5
16	159082.0
17	177542.0
18	184246.0
19	183046.5
20	176523.5
21	168979.5
22	163871.5
23	172183.0
24	201853.5
25	251108.5
26	319438.5
27	396129.5
28	475301.0
29	562785.0
30	646536.5
31	725870.0
32	807754.5
33	882178.0
34	948629.0
35	1003841.0
36	1054388.5
37	1092041.5
38	1100383.0
39	1096417.5
40	1101768.0
41	1104047.5
42	1093487.5
43	1086395.5
44	1080301.5
45	1074483.0
46	1067152.0
47	1051875.0
48	1029162.5
49	997770.0
50	963001.0
51	916952.5
52	857006.0
53	793010.5
54	727523.5
55	662221.0
56	600317.5
57	539150.5
58	475558.5
59	412944.0
60	349167.5
61	286028.0
62	228634.5
63	179077.5
64	137085.0
65	104212.0
66	79833.0
67	62296.5
68	49107.5
69	37227.5
70	27966.5
71	21501.0
72	16397.5
73	12349.0
74	9410.0
75	7390.5
76	5720.0
77	4212.5
78	2992.0
79	2135.0
80	1577.5
81	1191.5
82	905.0
83	620.0
84	411.5
85	311.5
86	251.5
87	197.0
88	142.0
89	99.0
90	76.0
91	60.0
92	46.5
93	29.5
94	12.5
95	7.5
96	7.0
97	6.5
98	3.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.10195162763245166
3	0.0
4	8.800629655716435E-5
5	0.0
6	0.0
7	0.0
8	1.0267401265002506E-4
9	0.0
10-11	1.1000787069645543E-5
12-13	9.167322558037953E-5
14-15	3.44691328182227E-4
16-17	6.728814757599857E-4
18-19	2.0168109627683495E-5
20-21	8.434588892178174E-5
22-23	1.8339525429567622E-6
24-25	0.006085452447977362
26-27	2.7536892184145144E-5
28-29	0.002324200462638992
30-31	0.001992802248528643
32-33	0.022320504177761973
34-35	0.015317021630684621
36-37	0.008279165503251484
38-39	0.003945286320553616
40-41	8.976939766126819E-4
42-43	2.7868712354014395E-4
44-45	0.0010410507554918728
46-47	0.0016025437801815633
48-49	0.0013382505783690755
50-51	3.541427829330802E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	4.476624774683336E-5
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	1.0971446037233087E-4
70-71	5.558728632873855E-4
72-73	6.6283705927301085E-6
74-75	4.9279597247707616E-5
76-77	2.6350882536488703E-4
78-79	1.773362152080452E-4
80-81	2.5730020925290383E-5
82-83	4.7575940418746774E-6
84-85	0.0
86-87	1.0117550139370488E-4
88-89	4.817053114542613E-4
90-91	5.173147309715088E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1255.0
20-21	4234.0
22-23	8281.0
24-25	15912.0
26-27	20214.0
28-29	33206.0
30-31	63866.0
32-33	82155.0
34-35	115099.0
36-37	146051.0
38-39	167154.0
40-41	187255.0
42-43	227676.0
44-45	271640.0
46-47	240366.0
48-49	220966.0
50-51	199489.0
52-53	193253.0
54-55	212111.0
56-57	229551.0
58-59	243842.0
60-61	248963.0
62-63	253822.0
64-65	272853.0
66-67	294259.0
68-69	303014.0
70-71	308011.0
72-73	306438.0
74-75	318902.0
76-77	291030.0
78-79	326863.0
80-81	353504.0
82-83	362654.0
84-85	382805.0
86-87	422080.0
88-89	478692.0
90-91	538246.0
92-93	626414.0
94-95	784872.0
96-97	1412129.0
98-99	2229419.0
100-101	1.387223E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.80410195654795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.82135723411487	73.54772720332616
2	7.3342800478647305	12.14616945722533
3	1.9864492406240897	4.934584363564332
4	0.8183191860961772	2.7104074127402877
5	0.39594906577391675	1.6393103405971658
6	0.21669150267879228	1.0765767168559381
7	0.12253889638444762	0.7102706288902467
8	0.08048962227524734	0.5331896711458892
9	0.052018358486782594	0.3876600113776627
>10	0.16932980812116272	2.0740899948888214
>50	0.0019647146877362935	0.10588345166991708
>100	5.559948814124516E-4	0.08427818367038972
>500	3.23809859757185E-5	0.018002131121027526
>1k	2.3947024654753675E-5	0.031850432926803056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1000787069645543E-5
2	0.0	2.2001574139291087E-5	0.0	1.4667716092860724E-5	2.2001574139291087E-5
3	0.0	2.9335432185721448E-5	0.0	1.4667716092860724E-5	2.2001574139291087E-5
4	0.0	7.333858046430363E-5	0.0	2.5668503162506268E-5	2.5668503162506268E-5
5	7.333858046430362E-6	7.333858046430363E-5	0.0	2.9335432185721448E-5	2.5668503162506268E-5
6	1.1000787069645543E-5	7.333858046430363E-5	0.0	3.300236120893663E-5	2.9335432185721448E-5
7	1.1000787069645543E-5	7.333858046430363E-5	0.0	3.666929023215181E-5	2.9335432185721448E-5
8	1.1000787069645543E-5	8.800629655716435E-5	0.0	4.033621925536699E-5	3.300236120893663E-5
9	1.1000787069645543E-5	1.4301023190539205E-4	0.0	8.800629655716435E-5	3.666929023215181E-5
10-11	2.2001574139291087E-5	1.4301023190539205E-4	0.0	1.4117676739378447E-4	4.033621925536699E-5
12-13	3.666929023215181E-5	1.4667716092860725E-4	0.0	1.5034408995182242E-4	4.950354181340494E-5
14-15	4.4003148278582174E-5	1.8517991567236665E-4	0.0	1.6134487702146796E-4	6.783818692948084E-5
16-17	6.233779339465809E-5	1.943472382304046E-4	0.0	1.6684527055629074E-4	7.70055094875188E-5
18-19	7.333858046430363E-5	2.2001574139291088E-4	0.0	1.8701338018397423E-4	8.617283204555675E-5
20-21	7.70055094875188E-5	2.5301810260184746E-4	0.0	2.530181026018475E-4	9.53401546035947E-5
22-23	8.617283204555675E-5	3.0435510892686E-4	0.0	4.400314827858217E-4	1.1000787069645544E-4
24-25	1.173417287428858E-4	3.336905411125815E-4	0.0	0.0010120724104073899	1.3200944483574652E-4
26-27	1.3017598032413893E-4	3.410243991590118E-4	0.0	0.0024073389037407664	1.3567637385896169E-4
28-29	1.3200944483574652E-4	3.44691328182227E-4	0.0	0.008113080463863588	1.3750983837056927E-4
30-31	1.5217755446343E-4	3.85027547437594E-4	0.0	0.009484511918546067	1.4117676739378447E-4
32-33	1.6134487702146796E-4	3.923614054840244E-4	0.0	0.012801249220044195	1.540110189750376E-4
34-35	1.7601259311432867E-4	4.290306957161762E-4	0.0	0.017362908924923884	1.576779479982528E-4
36-37	1.906803092071894E-4	4.7303384399475834E-4	0.0	0.025094628770373092	1.6684527055629074E-4
38-39	1.943472382304046E-4	4.987023471572647E-4	0.0	0.049023174111363754	1.723456640911135E-4
40-41	1.9801416725361977E-4	5.940425017608593E-4	0.0	0.07247501867933645	1.723456640911135E-4
42-43	1.9801416725361977E-4	6.178775404117581E-4	0.0	0.08774044420298124	1.723456640911135E-4
44-45	2.1268188334648048E-4	6.820487983180237E-4	0.0	0.10892979356363017	1.7784605762593628E-4
46-47	2.2368267041612605E-4	7.29718875619821E-4	0.0	0.13066551534873816	1.8884684469558182E-4
48-49	2.2734959943934122E-4	7.370527336662514E-4	0.0	0.15137083007832267	2.1634881236969568E-4
50-51	2.310165284625564E-4	7.993905270609094E-4	0.0	0.17332290067580036	2.2734959943934122E-4
52-53	2.4018385102059437E-4	8.103913141305549E-4	0.0	0.22486708849062453	2.585184961366703E-4
54-55	2.4201731553220195E-4	8.158917076653777E-4	0.0	0.2895407156730707	2.915208573456069E-4
56-57	2.4201731553220195E-4	8.360598172930612E-4	0.0	0.36201023395887233	3.061885734384676E-4
58-59	2.4201731553220195E-4	8.433936753394916E-4	0.0	0.4222102077329959	3.2085628953132833E-4
60-61	2.530181026018475E-4	8.580613914323524E-4	0.0	0.445123013734556	3.281901475777587E-4
62-63	2.566850316250627E-4	8.708956430136055E-4	0.0	0.4690423917529886	3.336905411125815E-4
64-65	2.566850316250627E-4	9.552350105475546E-4	0.0	0.47759733716414965	3.3552400562418907E-4
66-67	2.6035196064827786E-4	9.680692621288078E-4	0.0	0.4835230944656653	3.428578636706194E-4
68-69	2.7868660576435377E-4	9.772365846868456E-4	0.0	0.48938468050927486	3.5019172171704975E-4
70-71	2.841869992991765E-4	9.882373717564914E-4	0.0	0.4939390063561081	3.666929023215181E-4
72-73	2.860204638107841E-4	9.974046943145292E-4	0.0	0.4978277845852278	4.070291215768851E-4
74-75	2.860204638107841E-4	0.001021239732965428	0.0	0.500873169139008	4.106960506001003E-4
76-77	2.896873928339993E-4	0.0010707432747788328	0.0	0.5035646950420479	4.198633731581382E-4
78-79	2.915208573456069E-4	0.0010890779198949087	0.0	0.5056548445852806	4.25363766692961E-4
80-81	2.933543218572145E-4	0.0010927448489181238	0.0	0.5064414008607603	4.473653408322521E-4
82-83	2.9702125088042967E-4	0.0010927448489181238	0.0	0.5071912878460078	4.877015600876191E-4
84-85	3.061885734384676E-4	0.0011019121714761619	0.0	0.5075836492514918	4.877015600876191E-4
86-87	3.080220379500752E-4	0.001114746423057415	0.0	0.5078109988509312	4.913684891108342E-4
88	3.080220379500752E-4	0.0011477487842663516	0.0	0.5078330004250704	4.987023471572646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	35540	0.0	50.855522	1
GTATCAA	60745	0.0	32.81497	1
ATCAACG	76025	0.0	25.70617	3
TCAACGC	76310	0.0	25.631943	4
CAACGCA	77700	0.0	25.162664	5
AACGCAG	79440	0.0	24.66387	6
TATCAAC	80535	0.0	24.467209	2
ACGCAGA	91850	0.0	21.3134	7
CGCAGAG	92690	0.0	21.106798	8
GTGGTAT	15790	0.0	18.625288	1
GTACATG	76360	0.0	18.292812	1
TACATGG	75330	0.0	18.084606	2
GAGTACT	66745	0.0	18.024439	12-13
GCAGAGT	107810	0.0	17.838482	9
TGGTATC	15250	0.0	17.702744	2
AGAGTAC	99095	0.0	17.581375	10-11
ACATGGG	75925	0.0	17.574148	3
CAGAGTA	106485	0.0	17.309547	10-11
AGTACTT	73200	0.0	15.819031	12-13
GTACTTT	76565	0.0	15.6867485	14-15
>>END_MODULE