FastQCFastQC Report
Fri 27 May 2016
SRR2139977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8771875
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92000.046.3420451
GTATCAA155850.032.325121
TCAACGC187650.025.9398694
ATCAACG189450.025.6260323
CAACGCA191350.025.3938165
AACGCAG199100.024.44816
TATCAAC208250.023.7623842
ACGCAGA230550.021.1130627
CGCAGAG232800.020.8358978
GTACATG205750.019.1292151
TACATGG205250.018.7819352
ACATGGG204300.018.181773
AGAGTAC253550.017.97281810-11
GAGTACT169250.017.18304612-13
GCAGAGT280550.017.1227689
CATGGGG141400.016.7608534
CAGAGTA275350.016.3026319
AGTACTT192550.015.744612-13
GTACTTT195250.014.98310114-15
TATACTG54800.014.4418465
Produced by FastQC (version 0.11.5)