##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8771875
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86790190238689	31.0	31.0	34.0	30.0	34.0
2	32.062441952262205	33.0	31.0	34.0	30.0	34.0
3	32.115629554684716	33.0	31.0	34.0	30.0	34.0
4	35.80616230851443	37.0	35.0	37.0	35.0	37.0
5	35.676525258282865	37.0	35.0	37.0	33.0	37.0
6	35.69359116494478	37.0	35.0	37.0	33.0	37.0
7	35.5827902244389	37.0	35.0	37.0	33.0	37.0
8	35.5688022230139	37.0	35.0	37.0	33.0	37.0
9	37.13096014250089	39.0	37.0	39.0	33.0	39.0
10-11	37.03718070537941	39.0	37.0	39.0	33.0	39.0
12-13	37.00265918061988	39.0	37.0	39.0	33.0	39.0
14-15	38.16215455646598	40.0	37.0	41.0	33.0	41.0
16-17	38.165813295333095	40.0	37.5	41.0	33.0	41.0
18-19	38.199433872461704	40.0	37.5	41.0	33.0	41.0
20-21	38.172607188186596	40.0	38.0	41.0	33.0	41.0
22-23	38.09022775381706	40.0	37.0	41.0	33.0	41.0
24-25	38.012748416892705	40.0	37.0	41.0	33.0	41.0
26-27	37.843777424796954	40.0	37.0	41.0	32.5	41.0
28-29	37.7396829732249	40.0	37.0	41.0	32.0	41.0
30-31	37.62069726299656	39.5	37.0	41.0	32.0	41.0
32-33	37.7938502785171	40.0	37.0	41.0	33.0	41.0
34-35	37.77814593945001	40.0	37.0	41.0	33.0	41.0
36-37	37.69545353048453	40.0	37.0	41.0	32.5	41.0
38-39	37.50538204206022	40.0	37.0	41.0	32.0	41.0
40-41	37.52592263095603	40.0	37.0	41.0	32.0	41.0
42-43	37.496683579979745	40.0	36.5	41.0	32.0	41.0
44-45	37.48033136704876	39.5	36.5	41.0	32.0	41.0
46-47	37.515800049998944	40.0	37.0	41.0	32.0	41.0
48-49	37.44145560584822	40.0	36.0	41.0	31.5	41.0
50-51	37.36595313735485	39.0	36.0	41.0	31.0	41.0
52-53	37.23194904029947	39.0	36.0	41.0	31.0	41.0
54-55	37.14036824185273	39.0	36.0	40.5	31.0	41.0
56-57	37.00933590401121	39.0	35.0	40.0	31.0	41.0
58-59	36.87492713647797	39.0	35.0	40.0	31.0	41.0
60-61	36.69483546919803	38.5	35.0	40.0	31.0	41.0
62-63	36.47442509003106	38.0	35.0	40.0	30.0	41.0
64-65	36.28421564162605	38.0	35.0	40.0	30.0	41.0
66-67	36.01188060494212	37.5	34.0	40.0	29.5	41.0
68-69	35.74913905794567	37.0	34.0	40.0	29.0	41.0
70-71	35.47541180077252	36.5	34.0	39.0	29.0	41.0
72-73	35.18203574366325	36.0	34.0	39.0	29.0	40.0
74-75	34.8516015080071	36.0	34.0	38.5	29.0	40.0
76-77	33.80514486233034	35.0	32.0	37.0	28.0	39.0
78-79	34.14646720350089	35.0	33.0	37.0	29.0	39.0
80-81	34.038046890004516	35.0	33.0	37.0	29.0	39.0
82-83	33.810642526548264	35.0	33.0	36.0	29.0	38.5
84-85	33.56804993154508	35.0	33.0	36.0	29.0	37.0
86-87	33.33952598877899	35.0	33.0	35.5	29.0	37.0
88-89	33.10843008886708	35.0	32.5	35.0	29.0	36.5
90-91	32.991740524406126	34.0	32.0	35.0	29.0	36.0
92-93	32.91017136046357	34.0	32.0	35.0	29.0	36.0
94-95	32.85093142192838	34.0	32.0	35.0	29.0	36.0
96-97	32.76835240348812	34.0	32.0	35.0	29.0	35.0
98-99	32.899181039695556	34.0	32.0	35.0	29.0	35.0
100-101	32.43673295671603	34.0	31.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	60.0
21	453.0
22	2109.0
23	6789.0
24	17429.0
25	36271.0
26	63346.0
27	96966.0
28	135935.0
29	176996.0
30	224045.0
31	275239.0
32	337402.0
33	424114.0
34	552904.0
35	753539.0
36	1122633.0
37	1756970.0
38	1959721.0
39	806960.0
40	21984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.1524303526897	23.782087638047738	16.275323120769507	23.790158888493053
2	13.571599655759758	23.63053751569949	41.25775597908794	21.54010684945281
3	16.513111454430707	28.24924941905217	32.396380156937994	22.84125896957913
4	11.86843177769861	20.302728892055573	40.008778054862844	27.820061275382972
5	11.994094763092269	38.94039757748486	36.97696330602066	12.08854435340221
6	28.056977556109725	40.805164232276454	19.024290701816888	12.113567509796937
7	24.3698297114357	33.62031492696829	25.02118418240114	16.98867117919487
8	22.89121767011044	38.670500890630564	21.890405415033847	16.547876024225154
9	24.651286070537942	16.693888136800854	22.137798361239756	36.51702743142145
10-11	23.010604916280727	27.718680441752763	30.41862771642323	18.852086925543286
12-13	23.695885999287494	25.37102386889918	30.30905593159957	20.62403420021375
14-15	20.96774634841468	26.92692269326683	27.59273245457784	24.512598503740648
16-17	20.68446597791236	29.927438546490915	28.336228001425006	21.051867474171715
18-19	20.686215888849304	29.18888493053082	29.890747417171358	20.234151763448523
20-21	21.42893462678151	28.41994723006885	29.663887255718464	20.487230887431178
22-23	21.130779385409724	28.382989800155656	29.509940452830307	20.97629036160431
24-25	20.97731598737834	28.50031587225133	29.556211318906776	20.966156821463553
26-27	21.04500926984602	28.596485182907884	29.397078352647554	20.961427194598535
28-29	21.01086823266684	28.658651289903474	29.4528340481644	20.877646429265283
30-31	21.249561262396426	28.514438902385503	29.343873663225157	20.89212617199291
32-33	21.127716387882668	28.600205434052427	29.28201245222101	20.990065725843895
34-35	21.21541603397772	28.637675372420635	29.28721433706636	20.859694256535285
36-37	21.171350389122377	28.5848167312244	29.273461397057975	20.970371482595244
38-39	21.140194049752388	28.643746437683532	29.309193386902805	20.906866125661278
40-41	21.26774232547848	28.52977192789794	29.24567316078679	20.956812585836794
42-43	21.189126697299187	28.693845912850907	28.972875786631295	21.144151603218603
44-45	21.293790031686395	28.788936747051743	28.770847473588656	21.14642574767321
46-47	21.362133036356642	28.706653059479947	28.61296237353399	21.31825153062942
48-49	21.31430876857284	28.81850934038902	28.614128387445962	21.253053503592177
50-51	21.308289813312665	28.86709671496427	28.509498972745156	21.315114498977906
52-53	21.416649049773866	28.812556158626073	28.45367328276597	21.317121508834095
54-55	21.423838512826677	28.72749804310697	28.455059553014856	21.393603891051498
56-57	21.450201350300983	28.779657240863006	28.420095575271905	21.350045833564106
58-59	21.404628458589624	28.842566298420792	28.45486523384127	21.297940009148313
60-61	21.448093745742273	28.886149572387033	28.38345477347668	21.282301908394018
62-63	21.475933652573453	28.876948578920747	28.334349155523817	21.312768612981984
64-65	21.41939164315926	28.808644155060335	28.353005319274338	21.418958882506065
66-67	21.49018318534162	28.844261592212696	28.220837836733732	21.444717385711954
68-69	21.471908476347288	28.808920663829905	28.277735873155862	21.44143498666694
70-71	21.464872653503555	28.60839312836082	28.35990857988872	21.5668256382469
72-73	21.48146214042846	28.681029692474556	28.326454215544434	21.51105395155255
74-75	21.39180904824705	28.76417603444903	28.269407278521413	21.574607638782506
76-77	21.487353054318916	28.732784134105426	28.22378851365215	21.556074297923505
78-79	21.439873804776212	28.760189236922866	28.23153356970476	21.56840338859616
80-81	21.47164798838648	28.730608364103364	28.2446364102798	21.553107237230353
82-83	21.46027918123951	28.722463190567666	28.309122990246706	21.50813463794612
84-85	21.56642974004528	28.71519363357783	28.2265647652993	21.49181186107759
86-87	21.42604040545957	28.718818238006495	28.26970552763641	21.585435828897527
88-89	21.488380005155783	28.693795974892538	28.240619173516063	21.577204846435617
90-91	21.46738368876769	28.71439259351323	28.31194726119368	21.506276456525395
92-93	21.48571361221525	28.711783333056772	28.263681606699294	21.538821448028685
94-95	21.32639512114235	28.766260643704406	28.329318144707543	21.578026090445697
96-97	21.33423900876484	28.821443932566453	28.35704013805835	21.487276920610356
98-99	21.42322241177848	29.428727433471362	28.464091472652765	20.6839586820974
100-101	21.946506916409685	32.06666687018963	25.801963131561944	20.184863081838746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2391.0
1	1998.0
2	2313.0
3	3157.0
4	4027.5
5	5950.5
6	7571.0
7	8933.0
8	9882.5
9	9824.0
10	9262.5
11	8764.0
12	9790.0
13	13258.0
14	21292.5
15	32301.0
16	40821.5
17	45075.0
18	46447.0
19	46031.0
20	44671.5
21	43940.0
22	45583.5
23	51601.0
24	62680.5
25	79094.0
26	100294.0
27	124772.0
28	153190.5
29	183855.0
30	211826.5
31	238795.0
32	266139.5
33	289981.5
34	310839.0
35	327829.5
36	342480.5
37	353999.0
38	358840.0
39	359409.5
40	358951.0
41	357684.5
42	353455.0
43	348262.5
44	343452.0
45	339563.0
46	335498.5
47	329859.5
48	322625.5
49	312584.0
50	300669.5
51	286145.5
52	268682.0
53	250218.5
54	230454.0
55	208969.0
56	187944.5
57	166970.0
58	146033.5
59	125782.5
60	105408.5
61	85867.0
62	68346.5
63	53035.5
64	39772.5
65	29650.5
66	22445.5
67	17504.5
68	13718.5
69	10358.0
70	7717.5
71	5767.5
72	4324.0
73	3260.0
74	2519.5
75	1972.5
76	1472.5
77	1026.0
78	697.5
79	496.5
80	351.0
81	250.0
82	178.5
83	126.0
84	94.5
85	63.5
86	42.5
87	36.0
88	30.5
89	23.5
90	16.5
91	10.0
92	7.0
93	5.5
94	4.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010716066975418597
3	3.4200213751335945E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.002502421905483126
22-23	3.534553164639373E-4
24-25	0.0012259536322668258
26-27	0.011659398176547701
28-29	2.796611306128774E-4
30-31	0.002519660205823672
32-33	0.0028795661804741483
34-35	0.0016302500941412026
36-37	0.057150157847272204
38-39	0.023480916292475187
40-41	0.023520942880783744
42-43	0.003145751284343931
44-45	0.0016917739326267004
46-47	0.0036591455208745895
48-49	0.0030653794241133703
50-51	7.34680514766957E-4
52-53	9.545154423772184E-4
54-55	0.001270553437658106
56-57	0.011822603698234886
58-59	0.008942765983702687
60-61	0.004748458494415331
62-63	0.0024763052144077197
64-65	0.0012207576861875077
66-67	4.437578089954145E-4
68-69	8.048789386206578E-4
70-71	3.3357201522929704E-5
72-73	3.6433013653879086E-4
74-75	1.432963119350543E-4
76-77	0.003279236516418033
78-79	6.70949280008521E-4
80-81	5.807859847559261E-4
82-83	3.8766646326142363E-4
84-85	0.002590275654079164
86-87	0.0034385312365474846
88-89	0.04107201325450723
90-91	3.883930784697953E-4
92-93	5.195220490197744E-4
94-95	1.9777916152869366E-4
96-97	0.01600575752284822
98-99	1.1892083083528803E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	216.0
20-21	790.0
22-23	1557.0
24-25	2908.0
26-27	4282.0
28-29	7145.0
30-31	16174.0
32-33	21568.0
34-35	30688.0
36-37	47741.0
38-39	50694.0
40-41	53729.0
42-43	63636.0
44-45	75032.0
46-47	75669.0
48-49	69153.0
50-51	63662.0
52-53	63391.0
54-55	70068.0
56-57	73538.0
58-59	74847.0
60-61	73421.0
62-63	72161.0
64-65	77294.0
66-67	82852.0
68-69	83832.0
70-71	84321.0
72-73	83141.0
74-75	84689.0
76-77	86507.0
78-79	93969.0
80-81	95219.0
82-83	92820.0
84-85	94480.0
86-87	99505.0
88-89	107436.0
90-91	115563.0
92-93	123411.0
94-95	156542.0
96-97	204287.0
98-99	726013.0
100-101	5267924.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.58890912428295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.02597227245408	79.7286561332148
2	6.5393252878721455	11.455447367471175
3	1.429460250462015	3.756145919234765
4	0.49681268867471007	1.7406112576047943
5	0.21000002980206361	0.9196836763214832
6	0.1055899289851931	0.5549104016594141
7	0.05730852825083396	0.3513714031106088
8	0.037315364930615075	0.26147296862776787
9	0.025215234381239394	0.1987717385489285
>10	0.07172341489632855	0.9140748523929789
>50	9.702636767214549E-4	0.059030075993403604
>100	2.6912750149938946E-4	0.03862697143059573
>500	3.760811258682991E-5	0.021197234389248934
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.4200213751335945E-5	0.0
3	0.0	1.1400071250445315E-5	0.0	3.4200213751335945E-5	0.0
4	0.0	5.7000356252226574E-5	0.0	3.4200213751335945E-5	0.0
5	0.0	5.7000356252226574E-5	0.0	4.560028500178126E-5	0.0
6	7.98004987531172E-5	5.7000356252226574E-5	0.0	4.560028500178126E-5	0.0
7	9.120057000356252E-5	5.7000356252226574E-5	0.0	4.560028500178126E-5	0.0
8	9.120057000356252E-5	7.98004987531172E-5	0.0	4.560028500178126E-5	0.0
9	9.120057000356252E-5	1.3680085500534378E-4	0.0	5.7000356252226574E-5	1.1400071250445315E-5
10-11	1.0260064125400783E-4	1.596009975062344E-4	0.0	1.3680085500534378E-4	1.1400071250445315E-5
12-13	1.0260064125400783E-4	1.596009975062344E-4	0.0	1.5390096188101174E-4	1.1400071250445315E-5
14-15	1.0260064125400783E-4	2.1090131813323833E-4	0.0	1.8240114000712504E-4	2.8500178126113287E-5
16-17	1.596009975062344E-4	2.3940149625935163E-4	0.0	1.881011756323477E-4	3.4200213751335945E-5
18-19	1.9380121125757037E-4	2.565016031350196E-4	0.0	1.99501246882793E-4	4.560028500178126E-5
20-21	1.9380121125757037E-4	2.964018525115782E-4	0.0	2.0520128250801567E-4	4.560028500178126E-5
22-23	2.16601353758461E-4	3.3060206626291415E-4	0.0	2.33701460634129E-4	8.550053437833985E-5
24-25	2.16601353758461E-4	3.99002493765586E-4	0.0	2.6220163876024223E-4	1.1400071250445315E-4
26-27	2.16601353758461E-4	4.1040256501603134E-4	0.0	2.850017812611329E-4	1.1400071250445315E-4
28-29	2.16601353758461E-4	4.16102600641254E-4	0.0	4.3890274314214463E-4	1.1400071250445315E-4
30-31	2.16601353758461E-4	4.674029212682579E-4	0.0	9.234057712860706E-4	1.1400071250445315E-4
32-33	2.16601353758461E-4	4.7310295689348056E-4	0.0	0.002394014962593516	1.2540078375489848E-4
34-35	2.16601353758461E-4	5.529034556465977E-4	0.0	0.004001425008906306	1.7100106875667974E-4
36-37	2.280014250089063E-4	6.384039900249376E-4	0.0	0.006292839330245814	1.7100106875667974E-4
38-39	2.3940149625935163E-4	6.897043106519416E-4	0.0	0.010174563591022444	1.9380121125757037E-4
40-41	2.6220163876024223E-4	7.410046312789455E-4	0.0	0.01488849305308158	1.9380121125757037E-4
42-43	2.964018525115782E-4	7.638047737798361E-4	0.0	0.018946918418240113	1.9380121125757037E-4
44-45	2.964018525115782E-4	8.265051656572854E-4	0.0	0.02379194869967937	1.99501246882793E-4
46-47	3.3060206626291415E-4	8.778054862842893E-4	0.0	0.029560384752404703	2.1660135375846097E-4
48-49	3.648022800142501E-4	8.83505521909512E-4	0.0	0.03511221945137157	2.5080156750979695E-4
50-51	3.762023512646954E-4	9.861061631635196E-4	0.0	0.04071535447096544	2.5080156750979695E-4
52-53	3.762023512646954E-4	9.918061987887424E-4	0.0	0.04601638760242252	2.5080156750979695E-4
54-55	3.762023512646954E-4	9.918061987887424E-4	0.0	0.05217242607766298	2.565016031350196E-4
56-57	3.762023512646954E-4	0.0010089063056644104	0.0	0.05860206626291414	2.6220163876024223E-4
58-59	3.933024581403634E-4	0.001014606341289633	0.0	0.06486070537940862	2.6790167438546487E-4
60-61	4.1610260064125403E-4	0.0010260064125400784	0.0	0.0712219451371571	2.7360171001068756E-4
62-63	4.446027787673673E-4	0.0010260064125400784	0.0	0.07705878161738511	2.850017812611329E-4
64-65	4.446027787673673E-4	0.0011400071250445315	0.0	0.08372212326327039	2.907018168863555E-4
66-67	4.560028500178126E-4	0.00117990737442109	0.0	0.0901916636978981	3.078019237620235E-4
68-69	4.560028500178126E-4	0.001248307801923762	0.0	0.09717990737442109	3.192019950124688E-4
70-71	4.560028500178126E-4	0.0012711079444246527	0.0	0.10394014962593517	3.3060206626291415E-4
72-73	4.959030993943712E-4	0.0012882080513003207	0.0	0.1092810830067688	3.648022800142501E-4
74-75	5.073031706448166E-4	0.00135090844317777	0.0	0.11429141432133952	3.648022800142501E-4
76-77	5.187032418952618E-4	0.0014022087638047736	0.0	0.11909654435340221	3.648022800142501E-4
78-79	5.244032775204845E-4	0.0014193088706804416	0.0	0.1237477734235839	3.648022800142501E-4
80-81	5.358033487709298E-4	0.0014250089063056644	0.0	0.1266661916636979	3.762023512646954E-4
82-83	5.358033487709298E-4	0.0014364089775561098	0.0	0.1286042037762736	3.9900249376558606E-4
84-85	5.586034912718205E-4	0.0014421090131813323	0.0	0.1301660135375846	3.9900249376558606E-4
86-87	5.586034912718205E-4	0.0015048094050587816	0.0	0.13034841467759173	3.9900249376558606E-4
88-89	5.586034912718205E-4	0.0015219095119344496	0.0	0.1303940149625935	3.9900249376558606E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9200	0.0	46.342045	1
GTATCAA	15585	0.0	32.32512	1
TCAACGC	18765	0.0	25.939869	4
ATCAACG	18945	0.0	25.626032	3
CAACGCA	19135	0.0	25.393816	5
AACGCAG	19910	0.0	24.4481	6
TATCAAC	20825	0.0	23.762384	2
ACGCAGA	23055	0.0	21.113062	7
CGCAGAG	23280	0.0	20.835897	8
GTACATG	20575	0.0	19.129215	1
TACATGG	20525	0.0	18.781935	2
ACATGGG	20430	0.0	18.18177	3
AGAGTAC	25355	0.0	17.972818	10-11
GAGTACT	16925	0.0	17.183046	12-13
GCAGAGT	28055	0.0	17.122768	9
CATGGGG	14140	0.0	16.760853	4
CAGAGTA	27535	0.0	16.302631	9
AGTACTT	19255	0.0	15.7446	12-13
GTACTTT	19525	0.0	14.983101	14-15
TATACTG	5480	0.0	14.441846	5
>>END_MODULE