FastQCFastQC Report
Fri 27 May 2016
SRR2139976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11447607
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132600.054.624041
GTATCAA252350.044.225711
ATCAACG312950.035.0367163
TCAACGC319600.034.3628434
TATCAAC327050.033.8418352
CAACGCA332150.031.6914445
AACGCAG347600.030.520626
CTTATAC223850.028.1474151
ACGCAGA390300.027.0618177
CGCAGAG396850.026.6184658
TATACAC270000.025.0616783
GCAGAGT459000.022.755149
TTATACA292800.022.1186282
TACATGG306900.021.1475812
GTACATG330650.020.3131451
GAGTACT286900.019.83787512-13
ACATGGG325750.019.4724863
AGAGTAC425550.019.35061610-11
CAGAGTA450550.019.02471510-11
CATGGGG219600.018.947924
Produced by FastQC (version 0.11.5)