##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11447607
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814648598611047	33.0	31.0	34.0	30.0	34.0
2	31.495109501924727	33.0	31.0	34.0	30.0	34.0
3	31.80605160537045	33.0	31.0	34.0	30.0	34.0
4	32.99653971349645	37.0	35.0	37.0	28.0	37.0
5	34.44019776360247	37.0	35.0	37.0	28.0	37.0
6	35.13984066713681	37.0	35.0	37.0	32.0	37.0
7	35.276447645346316	37.0	35.0	37.0	32.0	37.0
8	35.418414870461575	37.0	35.0	37.0	32.0	37.0
9	37.182886606781665	39.0	37.0	39.0	33.0	39.0
10-11	37.052677559598266	39.0	37.0	39.0	33.0	39.0
12-13	36.94591620764061	39.0	37.0	39.0	32.5	39.0
14-15	38.28266798467138	40.0	38.0	41.0	33.0	41.0
16-17	38.27489924313439	40.0	38.0	41.0	32.5	41.0
18-19	38.21533758103331	40.0	38.0	41.0	33.0	41.0
20-21	38.28429129824757	40.0	38.0	41.0	32.5	41.0
22-23	38.322521295710956	40.0	38.0	41.0	32.5	41.0
24-25	38.20605803799923	40.0	38.0	41.0	32.0	41.0
26-27	38.112746307960904	40.0	38.0	41.0	32.0	41.0
28-29	38.107887874597566	40.0	38.0	41.0	32.0	41.0
30-31	38.04391440505627	40.0	37.0	41.0	32.0	41.0
32-33	37.943087076064046	40.0	37.0	41.0	32.0	41.0
34-35	37.82337228192377	40.0	37.0	41.0	31.0	41.0
36-37	37.77288877999164	40.0	37.0	41.0	31.0	41.0
38-39	37.679476192764405	40.0	37.0	41.0	31.0	41.0
40-41	37.62196604216669	40.0	37.0	41.0	31.0	41.0
42-43	37.60481994506737	40.0	37.0	41.0	31.0	41.0
44-45	37.53643005133161	40.0	36.0	41.0	30.5	41.0
46-47	37.40395152178908	40.0	36.0	41.0	30.0	41.0
48-49	37.562552838839785	40.0	36.0	41.0	31.0	41.0
50-51	37.50916630955656	40.0	36.0	41.0	30.0	41.0
52-53	37.456373949688064	40.0	36.0	41.0	30.5	41.0
54-55	37.329757730792835	39.0	35.5	41.0	30.0	41.0
56-57	37.19971428188272	39.0	35.0	41.0	30.0	41.0
58-59	37.09064648617749	39.0	35.0	41.0	30.5	41.0
60-61	36.88969932704065	39.0	35.0	41.0	30.5	41.0
62-63	36.71540787598185	38.5	35.0	40.5	30.0	41.0
64-65	36.46494372119622	38.0	35.0	40.0	30.0	41.0
66-67	36.25616435689158	37.5	35.0	40.0	30.0	41.0
68-69	36.00321194750652	37.0	34.0	40.0	30.0	41.0
70-71	35.75788851899183	37.0	34.0	39.0	30.0	41.0
72-73	35.45394771445508	36.0	34.0	39.0	30.0	41.0
74-75	35.2798344424573	36.0	34.0	39.0	30.0	40.5
76-77	35.082734658282945	35.5	34.0	38.5	30.0	40.0
78-79	34.82288429759484	35.0	34.0	37.0	30.0	39.0
80-81	34.567810040840044	35.0	34.0	37.0	30.0	39.0
82-83	34.306301799679666	35.0	34.0	36.0	30.0	39.0
84-85	34.092561380313136	35.0	34.0	36.0	30.0	37.0
86-87	33.90874006231925	35.0	34.0	36.0	29.0	37.0
88-89	33.769316227580035	35.0	34.0	35.0	29.0	37.0
90-91	33.62999471563904	35.0	34.0	35.0	29.0	36.0
92-93	33.59172678963441	35.0	34.0	35.0	29.0	36.0
94-95	33.56413666259685	35.0	34.0	35.0	29.0	36.0
96-97	33.566264129226795	35.0	34.0	35.0	29.5	36.0
98-99	33.60306478611292	35.0	34.0	35.0	30.0	35.5
100-101	33.218590767395085	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	10.0
17	78.0
18	474.0
19	1843.0
20	4771.0
21	10804.0
22	19776.0
23	33398.0
24	54078.0
25	85947.0
26	132164.0
27	190618.0
28	214951.0
29	177825.0
30	211395.0
31	258303.0
32	323529.0
33	423570.0
34	578485.0
35	817938.0
36	1248268.0
37	2163872.0
38	2829935.0
39	1583960.0
40	81612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.68348144725793	21.041288367079687	14.609725857989359	24.665504327673023
2	13.912833573464292	20.948393552783838	42.41114006117893	22.727632812572935
3	17.015078292697567	27.097535016829514	31.404308240876333	24.483078449596583
4	11.537736468322485	18.75733517959539	39.39955651873224	30.30537183334988
5	12.468433243226833	38.157581983592145	36.20192591175503	13.17205886142599
6	28.41347661843523	39.17523646272947	19.06931297912792	13.34197393970738
7	24.98707468234126	33.32841980488079	23.70840098900852	17.976104523769436
8	23.72101364642296	37.05360504464627	21.695399763547492	17.52998154538328
9	24.916412427959635	16.920815107129673	21.528891490119086	36.6338809747916
10-11	23.490307162666692	27.158609539802676	29.46340712545057	19.88767617208006
12-13	24.544905007424937	24.529711662915936	29.23804062984211	21.687342699817012
14-15	21.74799502655383	25.870576925957945	26.954075202954193	25.427352844534035
16-17	21.349288365498765	29.112319710961	27.524151371598737	22.014240551941498
18-19	21.254696025792402	28.005018748994747	29.342390721195024	21.39789450401783
20-21	22.24395647387392	27.330404764386373	29.028255148417042	21.397383613322663
22-23	21.819245949300885	27.415220745242124	29.02801572435453	21.737517581102463
24-25	21.66585229591256	27.391430367797852	29.335547896384018	21.60716943990557
26-27	21.8376850151436	27.617158976898175	29.093653912747275	21.45150209521095
28-29	21.387801924012066	27.482713615673056	29.20860628516792	21.920878175146964
30-31	22.07755584922603	27.412732702096143	29.16513951642927	21.344571932248556
32-33	22.063602558522792	27.318646454320817	29.327086580536637	21.290664406619754
34-35	21.387692300507258	27.477977748574318	29.6358550402673	21.49847491065112
36-37	21.8965379311834	27.394009551401304	29.20995454739338	21.499497970021917
38-39	21.459314397919684	27.366506402159374	29.446607791477035	21.7275714084439
40-41	21.71641132207209	27.29956902056933	29.39242073128923	21.59159892606935
42-43	21.86871399273006	27.220448689205806	29.318393880955945	21.592443437108187
44-45	21.56726152068931	27.737551191738895	28.93780600879014	21.75738127878165
46-47	22.002841682596106	27.217803826867353	28.421958426295014	22.357396064241524
48-49	21.87393553340341	27.329733590677037	28.604277792619758	22.192053083299793
50-51	21.520375411814268	27.66374155822416	28.613410534239677	22.2024724957219
52-53	21.741097804189966	27.74727140897862	28.65584001815471	21.85579076867671
54-55	21.72352426383439	27.70492456289701	28.35333564066498	22.218215532603622
56-57	22.010309122087612	27.993205974711806	27.962149938102055	22.03433496509853
58-59	21.55827287200816	28.34122460810238	28.538565202416144	21.561937317473316
60-61	21.80662954741814	28.56484374846942	27.8105047342715	21.818021969840938
62-63	21.73561152059083	28.8018287551549	27.81170213493127	21.650857589322996
64-65	21.45662447740289	28.68815986388047	27.911203012861225	21.94401264585542
66-67	21.624916370323323	28.669207337343767	27.66518935707748	22.04068693525543
68-69	21.755305143702376	28.50351234297399	27.89326506575618	21.847917447567454
70-71	21.773687650567712	27.9111270687148	28.084311240886635	22.230874039830848
72-73	21.82929105388776	27.890474215569522	27.960521678573112	22.3197130519696
74-75	21.6964638123	28.018107526985027	27.881679272176264	22.403749388538714
76-77	21.796452886208343	27.92853187084516	27.907430819041373	22.36758442390513
78-79	21.907877508685434	27.850052952370387	27.8847302574147	22.357339281529484
80-81	21.959184354724805	27.7635169438272	27.794168249248948	22.483130452199052
82-83	22.038956158166283	28.0172155985547	27.865253358150106	22.078574885128912
84-85	22.142757868915613	27.762005869010725	27.658574957547756	22.43666130452591
86-87	21.650081531485593	28.128093240392403	27.819935164420873	22.401890063701128
88-89	22.163005305389703	27.78267266475314	27.80556982962351	22.24875220023365
90-91	22.231782629184675	27.988384634569837	27.84739247084447	21.932440265401024
92-93	22.002287996101217	28.066538411595328	27.70154600338736	22.229627588916095
94-95	22.110556165952243	27.82053845692577	27.77292912714133	22.295976249980658
96-97	21.94053192944459	28.15202439854363	27.977910602495427	21.929533069516356
98-99	22.29533572183663	28.528563980158523	28.008444267990708	21.167656030014143
100-101	22.79950810537305	31.173138795840245	25.24987919688524	20.777473901901466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3111.0
1	2635.5
2	3511.0
3	4959.0
4	6495.0
5	10691.0
6	14066.5
7	16111.0
8	16789.0
9	15281.0
10	12811.5
11	10484.5
12	11444.0
13	16543.5
14	29072.0
15	46478.5
16	60680.5
17	68650.0
18	69629.5
19	65258.5
20	58424.0
21	52363.0
22	50682.0
23	56205.5
24	68367.0
25	86601.0
26	111895.5
27	139770.0
28	165977.5
29	189525.5
30	209804.0
31	230840.0
32	259049.5
33	290393.0
34	322154.0
35	350640.5
36	378715.0
37	402759.0
38	412280.0
39	420031.0
40	434616.5
41	443526.5
42	446571.5
43	453062.0
44	459135.0
45	465042.5
46	476696.5
47	494906.5
48	512342.0
49	504106.5
50	478823.0
51	456244.0
52	422284.0
53	388095.0
54	360627.5
55	334237.0
56	309872.5
57	282549.5
58	248599.5
59	213865.0
60	178836.5
61	139978.0
62	106245.5
63	80006.5
64	58408.0
65	42952.5
66	31768.5
67	24025.0
68	18422.0
69	13841.5
70	10385.5
71	7769.5
72	5817.0
73	4147.0
74	2977.0
75	2151.0
76	1459.0
77	933.5
78	626.5
79	443.5
80	311.0
81	216.5
82	156.0
83	106.5
84	76.0
85	55.0
86	42.0
87	36.0
88	29.5
89	22.0
90	15.0
91	10.0
92	5.5
93	2.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.74946606744973
3	0.0065865293943092205
4	8.57625528199911
5	3.668889052532988E-4
6	0.07508119382505007
7	0.026328646676986728
8	5.678042581301053E-4
9	2.7953440400251337E-4
10-11	0.2900082087024825
12-13	0.3032511510921016
14-15	1.0045767643840323E-4
16-17	1.4850265212633523E-4
18-19	0.47459263757045467
20-21	1.5289039065111083E-4
22-23	1.5729572986660534E-4
24-25	0.1723315920680263
26-27	0.15236623974471877
28-29	1.0939283650199164E-4
30-31	1.3144611184811E-4
32-33	1.1419689169237379E-4
34-35	3.436610680607087E-4
36-37	2.699078618629262E-4
38-39	0.0014779046357105295
40-41	0.001774616053302296
42-43	0.0011828095338060056
44-45	8.836980888297874E-4
46-47	0.0013256774844864663
48-49	0.0024134108638085745
50-51	3.990091242120567E-4
52-53	0.0016283683734696947
54-55	5.390635502496437E-4
56-57	0.009319791840981738
58-59	1.4094001155708094E-4
60-61	0.0027672143383823157
62-63	0.0015255573955803017
64-65	0.0014230694184286087
66-67	0.005130952202718796
68-69	0.002011481081300207
70-71	0.00251983272693011
72-73	0.0034740053851307186
74-75	6.778401046713218E-4
76-77	3.076102948854824E-4
78-79	7.480326332239295E-4
80-81	4.53190705743938E-4
82-83	0.0016001658801329168
84-85	2.1512677505772617E-4
86-87	5.271157509520169E-4
88-89	9.861956395185764E-5
90-91	3.820751949847282E-4
92-93	0.0
94-95	0.0
96-97	3.686767331800457E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	955.0
20-21	2438.0
22-23	3498.0
24-25	4954.0
26-27	6671.0
28-29	11441.0
30-31	26210.0
32-33	33224.0
34-35	43496.0
36-37	62892.0
38-39	75209.0
40-41	79879.0
42-43	92681.0
44-45	112638.0
46-47	112138.0
48-49	104186.0
50-51	92439.0
52-53	80467.0
54-55	86516.0
56-57	100086.0
58-59	76611.0
60-61	116055.0
62-63	114892.0
64-65	122369.0
66-67	132953.0
68-69	136165.0
70-71	120418.0
72-73	102973.0
74-75	101941.0
76-77	106259.0
78-79	109843.0
80-81	111943.0
82-83	105333.0
84-85	104631.0
86-87	106535.0
88-89	111685.0
90-91	115788.0
92-93	117205.0
94-95	127184.0
96-97	189023.0
98-99	941158.0
100-101	7044625.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.52760601460976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57246049132598	72.21100662681414
2	7.1685236137088975	11.688651377697715
3	2.1585442127161425	5.27942826418313
4	0.8823059577290314	2.877291700243016
5	0.4375486273749333	1.7836146052428428
6	0.24878478052505149	1.2169696541446535
7	0.15290470445590798	0.8726168151863127
8	0.10163842904380564	0.6629070239221805
9	0.06848626222870932	0.5025168903955917
>10	0.20483102097895822	2.4502711581590115
>50	0.002961332336741532	0.1589526127442973
>100	8.915727424882375E-4	0.13518814584591252
>500	5.9500039420724636E-5	0.03224173926231733
>1k	5.949479383561916E-5	0.12834338615890004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6206350375235626E-5
2	0.0	0.0	0.0	2.6206350375235626E-5	2.6206350375235626E-5
3	0.0	0.0	0.0	2.6206350375235626E-5	2.6206350375235626E-5
4	0.0	0.0	0.0	2.6206350375235626E-5	2.6206350375235626E-5
5	0.0	8.735450125078543E-6	0.0	2.6206350375235626E-5	2.6206350375235626E-5
6	0.0	8.735450125078543E-6	0.0	2.6206350375235626E-5	6.988360100062834E-5
7	0.0	8.735450125078543E-6	0.0	2.6206350375235626E-5	6.988360100062834E-5
8	0.0	8.735450125078543E-6	0.0	2.6206350375235626E-5	6.988360100062834E-5
9	0.0	8.735450125078543E-6	0.0	4.3677250625392715E-5	7.861905112570689E-5
10-11	0.0	8.735450125078543E-6	0.0	7.861905112570689E-5	7.861905112570689E-5
12-13	0.0	2.620635037523563E-5	0.0	8.735450125078543E-5	8.298677618824616E-5
14-15	0.0	7.861905112570689E-5	0.0	9.17222263133247E-5	1.9217990275172792E-4
16-17	0.0	1.1792857668856032E-4	0.0	1.0045767643840323E-4	2.445926035021992E-4
18-19	0.0	1.2666402681363887E-4	0.0	1.048254015009425E-4	3.013730293152097E-4
20-21	0.0	1.3103175187617814E-4	0.0	1.1792857668856032E-4	3.668889052532988E-4
22-23	0.0	1.3103175187617814E-4	0.0	1.3976720200125668E-4	3.930952556285344E-4
24-25	0.0	1.6160582731395304E-4	0.0	2.3148942831458137E-4	4.3240478119138784E-4
26-27	0.0	1.834444526266494E-4	0.0	4.0619843081615226E-4	4.5861113156662347E-4
28-29	0.0	1.8781217768918868E-4	0.0	0.001528703771888745	4.717143067542413E-4
30-31	0.0	1.9217990275172795E-4	0.0	0.005734823007114063	4.848174819418591E-4
32-33	0.0	1.9217990275172795E-4	0.0	0.01593346102814326	5.284947325672519E-4
34-35	8.735450125078543E-6	2.09650803001885E-4	0.0	0.03039499871021079	5.678042581301053E-4
36-37	8.735450125078543E-6	2.445926035021992E-4	0.0	0.050949512854520596	5.852751583802624E-4
38-39	8.735450125078543E-6	2.882698541275919E-4	0.0	0.08357205134662643	6.11481508755498E-4
40-41	8.735450125078543E-6	3.232116546279061E-4	0.0	0.1461528160426891	6.202169588805765E-4
42-43	8.735450125078543E-6	3.494180050031417E-4	0.0	0.17354281990987286	6.245846839431158E-4
44-45	8.735450125078543E-6	3.494180050031417E-4	0.0	0.20732717326861413	6.464233092558122E-4
46-47	8.735450125078543E-6	3.494180050031417E-4	0.0	0.24034280701634847	6.813651097561264E-4
48-49	8.735450125078543E-6	3.53785730065681E-4	0.0	0.2679468294115967	7.07571460131362E-4
50-51	8.735450125078543E-6	3.5815345512822027E-4	0.0	0.2908468119144901	7.250423603815191E-4
52-53	8.735450125078543E-6	3.6252118019075954E-4	0.0	0.32887222630895696	7.425132606316762E-4
54-55	8.735450125078543E-6	3.974629806910737E-4	0.0	0.4178165794825067	7.643518859443725E-4
56-57	8.735450125078543E-6	4.01830705753613E-4	0.0	0.5099799460271478	7.730873360694511E-4
58-59	8.735450125078543E-6	4.105661558786915E-4	0.0	0.5544783289642979	7.774550611319903E-4
60-61	8.735450125078543E-6	4.4550795637900566E-4	0.0	0.8933963229170953	7.774550611319903E-4
62-63	8.735450125078543E-6	4.542434065040842E-4	0.0	0.9002885930657822	7.905582363196082E-4
64-65	8.735450125078543E-6	4.542434065040842E-4	0.0	0.9065431753553385	7.992936864446866E-4
66-67	8.735450125078543E-6	4.8044975687931983E-4	0.0	0.9121338634353887	8.123968616323044E-4
68-69	8.735450125078543E-6	4.8044975687931983E-4	0.0	0.9179953504693165	8.255000368199222E-4
70-71	8.735450125078543E-6	4.848174819418591E-4	0.0	0.923450639072428	8.473386621326186E-4
72-73	8.735450125078543E-6	4.891852070043984E-4	0.0	0.9281852530402206	9.521640636335611E-4
74-75	8.735450125078543E-6	4.891852070043984E-4	0.0	0.9317405812411275	0.001013312214509111
76-77	8.735450125078543E-6	4.979206571294769E-4	0.0	0.9349552268871564	0.0010264153896967288
78-79	8.735450125078543E-6	5.022883821920161E-4	0.0	0.9374055206472409	0.001030783114759268
80-81	8.735450125078543E-6	5.15391557379634E-4	0.0	0.9386590577401897	0.0010657249152595822
82-83	8.735450125078543E-6	5.241270075047126E-4	0.0	0.9391919201978195	0.0011399762413227498
84-85	8.735450125078543E-6	5.241270075047126E-4	0.0	0.9395631768281354	0.001222963017510996
86-87	8.735450125078543E-6	5.241270075047126E-4	0.0	0.9396723699546988	0.001222963017510996
88-89	8.735450125078543E-6	5.328624576297911E-4	0.0	0.9397509890058245	0.0012579048180113102
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13260	0.0	54.62404	1
GTATCAA	25235	0.0	44.22571	1
ATCAACG	31295	0.0	35.036716	3
TCAACGC	31960	0.0	34.362843	4
TATCAAC	32705	0.0	33.841835	2
CAACGCA	33215	0.0	31.691444	5
AACGCAG	34760	0.0	30.52062	6
CTTATAC	22385	0.0	28.147415	1
ACGCAGA	39030	0.0	27.061817	7
CGCAGAG	39685	0.0	26.618465	8
TATACAC	27000	0.0	25.061678	3
GCAGAGT	45900	0.0	22.75514	9
TTATACA	29280	0.0	22.118628	2
TACATGG	30690	0.0	21.147581	2
GTACATG	33065	0.0	20.313145	1
GAGTACT	28690	0.0	19.837875	12-13
ACATGGG	32575	0.0	19.472486	3
AGAGTAC	42555	0.0	19.350616	10-11
CAGAGTA	45055	0.0	19.024715	10-11
CATGGGG	21960	0.0	18.94792	4
>>END_MODULE