FastQCFastQC Report
Fri 27 May 2016
SRR2139975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876667
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101850.055.03121
GTATCAA190250.042.204041
ATCAACG228200.034.470783
TCAACGC234100.033.681154
TATCAAC242700.032.8691142
CAACGCA241050.031.1424065
AACGCAG255400.029.5057536
ACGCAGA283250.026.4926517
CGCAGAG288100.026.05368
GCAGAGT327950.022.717069
TACATGG232150.021.4564192
ACATGGG238450.020.5786783
GTGGTAT53550.020.2097661
GTACATG260600.020.1114081
GAGTACT205850.019.75179712-13
CAGAGTA326750.019.2844910-11
CATGGGG154300.019.1168334
TGGTATC53700.018.9212342
AGAGTAC313850.018.72130610-11
GTACTTT233450.017.3869514-15
Produced by FastQC (version 0.11.5)