##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8876667
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862471015303377	33.0	31.0	34.0	30.0	34.0
2	31.4830951752499	33.0	31.0	34.0	30.0	34.0
3	31.82996388171371	34.0	31.0	34.0	30.0	34.0
4	32.77313962549231	37.0	35.0	37.0	22.0	37.0
5	34.35417178542352	37.0	35.0	37.0	25.0	37.0
6	35.17631054538826	37.0	35.0	37.0	32.0	37.0
7	35.31400783649989	37.0	35.0	37.0	32.0	37.0
8	35.45679397458528	37.0	35.0	37.0	32.0	37.0
9	37.227805098467705	39.0	37.0	39.0	33.0	39.0
10-11	37.08457802911836	39.0	37.0	39.0	33.0	39.0
12-13	36.98163815314915	39.0	37.0	39.0	32.5	39.0
14-15	38.36212922034814	40.0	38.0	41.0	33.0	41.0
16-17	38.35600096297405	40.0	38.0	41.0	33.0	41.0
18-19	38.277895126628046	40.0	38.0	41.0	33.0	41.0
20-21	38.351489796076294	40.0	38.0	41.0	33.0	41.0
22-23	38.4002163553328	40.0	38.0	41.0	33.0	41.0
24-25	38.27326359186176	40.0	38.0	41.0	32.0	41.0
26-27	38.185257202020026	40.0	38.0	41.0	32.0	41.0
28-29	38.20676238706217	40.0	38.0	41.0	32.0	41.0
30-31	38.157193518609674	40.0	38.0	41.0	32.0	41.0
32-33	38.0554193177537	40.0	37.0	41.0	32.0	41.0
34-35	37.94095483528223	40.0	37.0	41.0	31.0	41.0
36-37	37.8983665132298	40.0	37.0	41.0	31.0	41.0
38-39	37.82571133828823	40.0	37.0	41.0	31.0	41.0
40-41	37.75824715488116	40.0	37.0	41.0	31.0	41.0
42-43	37.73758420103255	40.0	37.0	41.0	31.0	41.0
44-45	37.676078961411484	40.0	37.0	41.0	31.0	41.0
46-47	37.55291209001089	40.0	37.0	41.0	30.0	41.0
48-49	37.72140947376894	40.0	37.0	41.0	31.0	41.0
50-51	37.661474344962684	40.0	37.0	41.0	31.0	41.0
52-53	37.6092593344505	40.0	36.0	41.0	31.0	41.0
54-55	37.49742026073508	40.0	36.0	41.0	30.0	41.0
56-57	37.367655518548915	39.0	36.0	41.0	30.0	41.0
58-59	37.27798623117282	39.0	35.5	41.0	30.5	41.0
60-61	37.08235778774363	39.0	35.0	41.0	31.0	41.0
62-63	36.91634218238466	39.0	35.0	41.0	31.0	41.0
64-65	36.684349670377856	38.5	35.0	40.5	30.0	41.0
66-67	36.48415924270557	38.0	35.0	40.0	30.0	41.0
68-69	36.230643181531086	37.5	35.0	40.0	30.0	41.0
70-71	35.981163720265855	37.0	34.0	39.5	30.0	41.0
72-73	35.67225413291068	36.5	34.0	39.0	30.0	41.0
74-75	35.4844071791794	36.0	34.0	39.0	30.0	41.0
76-77	35.2603465627554	36.0	34.0	38.5	30.0	40.0
78-79	34.97256455103064	35.0	34.0	37.0	30.0	39.0
80-81	34.688348219194296	35.0	34.0	37.0	30.0	39.0
82-83	34.407340912053826	35.0	34.0	36.5	30.0	39.0
84-85	34.1752918192081	35.0	34.0	36.0	30.0	37.0
86-87	33.98567812602788	35.0	34.0	36.0	30.0	37.0
88-89	33.83216877997138	35.0	34.0	35.5	29.0	37.0
90-91	33.69652256525413	35.0	34.0	35.0	29.0	36.0
92-93	33.65446251646621	35.0	34.0	35.0	29.0	36.0
94-95	33.613929634442655	35.0	34.0	35.0	29.5	36.0
96-97	33.60052479098202	35.0	34.0	35.0	30.0	36.0
98-99	33.61971172700648	35.0	34.0	35.0	30.0	35.5
100-101	33.23042451051032	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	55.0
18	345.0
19	1224.0
20	3238.0
21	7476.0
22	13796.0
23	23518.0
24	38748.0
25	63012.0
26	100494.0
27	150480.0
28	174796.0
29	133480.0
30	153412.0
31	190541.0
32	238190.0
33	313494.0
34	429877.0
35	605982.0
36	922445.0
37	1627153.0
38	2334458.0
39	1281426.0
40	69024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.579551649284575	21.16639049318849	14.550078312051134	24.703979545475796
2	13.670570767414924	21.13495807462215	42.75751783924741	22.436953318715513
3	16.881199435203097	27.224794732738246	31.449730630903556	24.4442752011551
4	11.366323988766286	19.01908953327541	40.03816986959083	29.576416608367467
5	12.252159631283535	38.384607854038315	36.568761278913136	12.794471235765009
6	27.943217379677517	39.633708446344876	19.36030037300855	13.06277380096906
7	24.809625421620982	33.36819890368403	24.043349150177075	17.77882652451791
8	23.26412636534371	37.38706134582218	22.086435915688995	17.26237637314511
9	24.796662789070723	17.069803711632133	21.847628278917437	36.2859052203797
10-11	23.215914681775196	27.380233732675723	29.854009475306885	19.549842110242192
12-13	24.263355551256392	24.687401322517758	29.627465605342746	21.4217775208831
14-15	21.435088520927582	26.080181302467665	27.27397518300243	25.210754993602325
16-17	21.044724970630817	29.32560609563155	27.84125704150619	21.78841189223144
18-19	21.02919321640463	28.37209136394246	29.56596034227389	21.032755077379022
20-21	21.975642865685586	27.656664490088307	29.23474107496306	21.13295156926305
22-23	21.426790084334453	27.8172977363711	29.205684518188324	21.550227661106124
24-25	21.334800784919818	27.84362322674149	29.431178222292598	21.390397766046096
26-27	21.42756758985668	28.022777455493188	29.236974446252628	21.312680508397506
28-29	21.307917049726665	27.89588704283511	29.39469138787092	21.4015045195673
30-31	21.412814917182	27.554643339401046	29.63432369066578	21.39821805275117
32-33	21.06721441606475	28.04061191245092	29.310589185629894	21.581584485854442
34-35	21.369827146526177	27.844494349348718	29.38946385548327	21.396214648641834
36-37	21.27041444792037	27.799284856596312	29.66613161807512	21.2641690774082
38-39	21.109787917691694	27.809425720986606	29.603712336921827	21.477074024399865
40-41	21.439306359380915	27.666362384736576	29.491137603880947	21.403193652001562
42-43	21.715587510738114	27.601233469221288	29.369531861361196	21.313647158679398
44-45	21.330875979650724	28.073366995097686	29.073091538083467	21.522665487168126
46-47	21.648018669659066	27.58745356430252	28.659790510350806	22.104737255687606
48-49	21.521817294466096	27.77313457645346	28.776119798654232	21.92892833042621
50-51	21.155295434427167	27.886845381256446	28.957820746925268	22.000038437391122
52-53	21.315815253407095	27.8783864319802	28.91549558484334	21.890302729769363
54-55	21.430737510121045	27.613327449882963	28.747565568391526	22.20836947160447
56-57	21.703715862909206	27.69405501067031	28.52530376606467	22.076925360355812
58-59	21.139730390590373	27.86537444658288	29.204121753048966	21.79077340977778
60-61	21.509204615844574	28.038720347520524	28.662265771426434	21.789809265208472
62-63	20.987348671236106	28.72039529330844	28.67120881190485	21.621047223550605
64-65	20.584062839971338	28.860176747770268	28.627696104846706	21.92806430741169
66-67	20.802134336579247	29.277165230699815	28.106891624644565	21.813808808076374
68-69	21.0319652673434	29.1645738900914	27.959430677230547	21.84403016533466
70-71	21.06146680308886	28.452700465597182	28.049105461980496	22.43672726933347
72-73	21.03064079730561	28.109252394557377	28.088625999402673	22.77148080873434
74-75	21.305080148230548	27.885032198357667	28.108484935032802	22.701402718378986
76-77	21.557382233210518	27.861629561372148	27.957327533227165	22.62366067219017
78-79	21.823693855624686	27.77451773816274	27.384335042629047	23.017453363583527
80-81	21.671101652020813	27.994202620874866	27.351124560639935	22.983571166464394
82-83	21.69948983972119	28.11255250656968	27.485590846152647	22.70236680755648
84-85	21.916356669390204	28.091481175719245	27.32497249319941	22.667189661691133
86-87	21.831498419231774	28.49725981065177	27.279975120456545	22.39126664965991
88-89	22.184625585719438	28.16549661826146	27.581630735770258	22.068247060248847
90-91	21.998133107934294	28.202932637536293	27.83521370034177	21.963720554187642
92-93	21.777567340224127	27.849568276017244	27.587717566312044	22.78514681744658
94-95	21.56303450006026	27.87279791170224	28.198948974761112	22.365218613476383
96-97	21.166931426296028	28.116612505179035	28.499202649209042	22.217253419315895
98-99	21.44996135897051	28.319199176103698	28.734123693791148	21.496715771134646
100-101	22.283878541167322	31.164646619174707	25.998401265563608	20.55307357409436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2129.0
1	1821.0
2	2344.0
3	3313.5
4	4528.5
5	7602.0
6	10020.5
7	11211.5
8	11307.5
9	10078.5
10	8506.5
11	7210.5
12	8031.0
13	11638.0
14	20241.0
15	32217.0
16	42003.5
17	47349.5
18	48000.5
19	45085.0
20	40801.5
21	37068.5
22	36028.0
23	40415.5
24	49701.5
25	63353.5
26	83156.0
27	105686.5
28	127271.5
29	146315.5
30	161978.5
31	179627.0
32	201697.5
33	225743.5
34	252930.5
35	279095.5
36	304178.5
37	322952.5
38	330365.5
39	337814.5
40	350805.0
41	359411.0
42	362304.5
43	368433.5
44	376237.0
45	386671.5
46	401951.0
47	409396.0
48	401535.5
49	381236.5
50	355376.0
51	332194.5
52	308133.5
53	284888.5
54	261103.0
55	235109.0
56	210857.5
57	186216.0
58	160233.0
59	136162.0
60	111905.0
61	85530.0
62	63109.5
63	46570.5
64	33898.0
65	24772.5
66	18242.0
67	13721.5
68	10248.5
69	7325.5
70	5441.5
71	3925.5
72	2804.5
73	2013.0
74	1383.0
75	966.5
76	687.5
77	449.0
78	318.0
79	248.0
80	184.5
81	135.5
82	104.5
83	72.5
84	47.0
85	35.5
86	34.0
87	30.5
88	20.0
89	12.5
90	10.0
91	8.0
92	7.0
93	3.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.9864212547344626
3	0.00626361223193345
4	9.35632709889872
5	4.055576265280651E-4
6	0.07467893072929287
7	0.029098759703388672
8	6.308674190436569E-4
9	3.717611576507263E-4
10-11	0.3271272877533876
12-13	0.3377450117256849
14-15	1.0138940663201628E-4
16-17	1.6898234438669378E-4
18-19	0.5296244637767756
20-21	2.760384227457463E-4
22-23	2.253842124669925E-4
24-25	0.19433246300150347
26-27	0.17956263085473076
28-29	1.1283742975517381E-4
30-31	1.4122333413943614E-4
32-33	9.625920860888004E-5
34-35	3.918920599772055E-4
36-37	3.193969238540053E-4
38-39	0.0014172090025246977
40-41	0.0017231907822317938
42-43	0.0011783563681686822
44-45	0.0011123538901210972
46-47	0.001566976044898062
48-49	0.002734498141974188
50-51	4.578727820958729E-4
52-53	0.001819712030571162
54-55	5.836400584758702E-4
56-57	0.011592117628179598
58-59	1.466448573176089E-4
60-61	0.0027379506230863506
62-63	0.0015322076879606314
64-65	0.0015745627937837766
66-67	0.004591986735446117
68-69	0.0021369841605060483
70-71	0.0024343419471185225
72-73	0.003586209064628837
74-75	7.009970982725829E-4
76-77	3.7123493528632605E-4
78-79	7.375154511194221E-4
80-81	4.2853255454683756E-4
82-83	0.0016582680404869292
84-85	2.9736993286944566E-4
86-87	3.797842152078068E-4
88-89	1.5960126380986208E-4
90-91	3.3809741997858813E-4
92-93	0.0
94-95	0.0
96-97	5.37582714780422E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	729.0
20-21	1672.0
22-23	2398.0
24-25	3231.0
26-27	4615.0
28-29	8244.0
30-31	19714.0
32-33	25012.0
34-35	33369.0
36-37	48065.0
38-39	39393.0
40-41	60391.0
42-43	69230.0
44-45	83006.0
46-47	83350.0
48-49	77126.0
50-51	69484.0
52-53	18560.0
54-55	19928.0
56-57	20727.0
58-59	22305.0
60-61	84433.0
62-63	87845.0
64-65	95768.0
66-67	103958.0
68-69	106962.0
70-71	95928.0
72-73	81995.0
74-75	80922.0
76-77	84227.0
78-79	87704.0
80-81	89072.0
82-83	84684.0
84-85	83888.0
86-87	84598.0
88-89	88724.0
90-91	91828.0
92-93	92719.0
94-95	100576.0
96-97	149148.0
98-99	774691.0
100-101	5616448.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.24823012928192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08997396179812	71.57154476530627
2	7.5715615557624165	12.303519514412173
3	2.246184405140348	5.474955223849416
4	0.9153067707629235	2.974682205993436
5	0.44498333435172693	1.8077054176552156
6	0.2501040760770072	1.2192308117625668
7	0.14837112907778846	0.8438424147897484
8	0.0953057688531627	0.619474003234393
9	0.06026901752937439	0.4407075905303104
>10	0.1737119439451679	2.0665729946104
>50	0.002277487453897277	0.12916338178049439
>100	0.001625715752527074	0.24827958936298195
>500	1.9989773540569303E-4	0.1155570542318875
>1k	1.2493576010148198E-4	0.18476503248074394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.3796468877338756E-5	0.0	0.0	0.0
3	0.0	3.3796468877338756E-5	0.0	1.1265489625779585E-5	0.0
4	0.0	5.632744812889793E-5	0.0	1.1265489625779585E-5	0.0
5	1.1265489625779585E-5	5.632744812889793E-5	0.0	1.1265489625779585E-5	0.0
6	1.1265489625779585E-5	5.632744812889793E-5	0.0	1.1265489625779585E-5	1.1265489625779585E-5
7	1.1265489625779585E-5	5.632744812889793E-5	0.0	2.253097925155917E-5	1.1265489625779585E-5
8	1.1265489625779585E-5	5.632744812889793E-5	0.0	3.3796468877338756E-5	4.506195850311834E-5
9	1.1265489625779585E-5	9.012391700623668E-5	0.0	6.759293775467751E-5	4.506195850311834E-5
10-11	3.942921369022855E-5	9.012391700623668E-5	0.0	7.88584273804571E-5	4.506195850311834E-5
12-13	6.759293775467751E-5	9.012391700623668E-5	0.0	7.88584273804571E-5	4.506195850311834E-5
14-15	6.759293775467751E-5	9.012391700623668E-5	0.0	1.1265489625779585E-4	6.759293775467751E-5
16-17	1.1265489625779585E-4	9.012391700623668E-5	0.0	1.3518587550935502E-4	1.0138940663201627E-4
18-19	1.520841099480244E-4	1.1265489625779585E-4	0.0	1.4081862032224482E-4	1.1265489625779585E-4
20-21	1.577168547609142E-4	1.464513651351346E-4	0.0	1.63349599573804E-4	1.1265489625779585E-4
22-23	2.253097925155917E-4	2.0277881326403254E-4	0.0	2.0277881326403254E-4	1.4081862032224482E-4
24-25	3.26699199147608E-4	2.1404430288981212E-4	0.0	2.929027302702692E-4	1.577168547609142E-4
26-27	3.4923017839916715E-4	2.1404430288981212E-4	0.0	5.576417364760895E-4	1.577168547609142E-4
28-29	3.6049566802494673E-4	2.1404430288981212E-4	0.0	0.0018137438297505136	1.577168547609142E-4
30-31	3.830266472765059E-4	2.5910626139293046E-4	0.0	0.007294404532692282	1.577168547609142E-4
32-33	3.830266472765059E-4	2.5910626139293046E-4	0.0	0.020767930125124665	1.8024783401247337E-4
34-35	3.830266472765059E-4	2.872699854573794E-4	0.0	0.039631992503492586	1.9151332363825295E-4
36-37	4.844160539085222E-4	3.210664543347182E-4	0.0	0.06551445491872118	1.9151332363825295E-4
38-39	4.844160539085222E-4	3.26699199147608E-4	0.0	0.11283514409180834	2.0277881326403254E-4
40-41	4.90048798721412E-4	3.6049566802494673E-4	0.0	0.3962748630764227	2.1404430288981212E-4
42-43	4.956815435343018E-4	3.6049566802494673E-4	0.0	0.4382050154635743	2.1967704770270191E-4
44-45	4.956815435343018E-4	3.773939024636161E-4	0.0	0.4981599512519733	2.253097925155917E-4
46-47	4.956815435343018E-4	3.942921369022855E-4	0.0	0.5608467682746239	2.365752821413713E-4
48-49	5.351107572245304E-4	3.999248817151753E-4	0.0	0.6136143216817753	2.5910626139293046E-4
50-51	5.97070950166318E-4	4.3372135059251404E-4	0.0	0.6614419578880226	2.5910626139293046E-4
52-53	6.196019294178772E-4	4.3935409540540383E-4	0.0	0.8405745084275438	2.7037175101871005E-4
54-55	6.308674190436568E-4	4.3935409540540383E-4	0.0	1.4462466599231445	2.8163724064448963E-4
56-57	6.53398398295216E-4	4.506195850311834E-4	0.0	2.101391209110356	2.872699854573794E-4
58-59	6.984603567983343E-4	4.506195850311834E-4	0.0	2.780480556497163	2.98535475083159E-4
60-61	7.32256825675673E-4	4.506195850311834E-4	0.0	3.287191014375103	3.041682198960488E-4
62-63	7.435223153014526E-4	4.61885074656963E-4	0.0	3.2972004019076078	3.041682198960488E-4
64-65	7.604205497401221E-4	5.182125227858609E-4	0.0	3.3035823017806116	3.041682198960488E-4
66-67	7.716860393659015E-4	5.238452675987507E-4	0.0	3.3100205291017453	3.098009647089386E-4
68-69	7.773187841787914E-4	5.294780124116405E-4	0.0	3.3160250350722853	3.154337095218284E-4
70-71	7.773187841787914E-4	5.294780124116405E-4	0.0	3.321263487748273	3.26699199147608E-4
72-73	7.829515289916812E-4	5.294780124116405E-4	0.0	3.3255274755716306	3.3796468877338756E-4
74-75	7.88584273804571E-4	5.294780124116405E-4	0.0	3.3289071224593645	3.3796468877338756E-4
76-77	7.88584273804571E-4	5.463762468503098E-4	0.0	3.331824884272441	3.3796468877338756E-4
78-79	7.88584273804571E-4	5.632744812889793E-4	0.0	3.335001752346911	3.3796468877338756E-4
80-81	7.998497634303506E-4	5.632744812889793E-4	0.0	3.3365113279567655	3.5486292321205694E-4
82-83	7.998497634303506E-4	5.632744812889793E-4	0.0	3.3370408059691776	3.717611576507263E-4
84-85	7.998497634303506E-4	5.858054605405384E-4	0.0	3.3372999122305704	3.717611576507263E-4
86-87	7.998497634303506E-4	5.97070950166318E-4	0.0	3.3375026910438343	3.717611576507263E-4
88-89	7.998497634303506E-4	6.871948671725547E-4	0.0	3.3375477530023376	3.830266472765059E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10185	0.0	55.0312	1
GTATCAA	19025	0.0	42.20404	1
ATCAACG	22820	0.0	34.47078	3
TCAACGC	23410	0.0	33.68115	4
TATCAAC	24270	0.0	32.869114	2
CAACGCA	24105	0.0	31.142406	5
AACGCAG	25540	0.0	29.505753	6
ACGCAGA	28325	0.0	26.492651	7
CGCAGAG	28810	0.0	26.0536	8
GCAGAGT	32795	0.0	22.71706	9
TACATGG	23215	0.0	21.456419	2
ACATGGG	23845	0.0	20.578678	3
GTGGTAT	5355	0.0	20.209766	1
GTACATG	26060	0.0	20.111408	1
GAGTACT	20585	0.0	19.751797	12-13
CAGAGTA	32675	0.0	19.28449	10-11
CATGGGG	15430	0.0	19.116833	4
TGGTATC	5370	0.0	18.921234	2
AGAGTAC	31385	0.0	18.721306	10-11
GTACTTT	23345	0.0	17.38695	14-15
>>END_MODULE