FastQCFastQC Report
Fri 27 May 2016
SRR2139974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12800142
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150250.054.982751
GTATCAA289150.042.5534861
ATCAACG355450.033.9523933
TCAACGC363350.033.287214
TATCAAC367800.033.0161742
CAACGCA373650.031.0044235
AACGCAG395400.029.5632536
CTTATAC278300.028.5935121
ACGCAGA433550.026.846227
TATACAC321900.026.1383443
CGCAGAG453300.025.7079688
TTATACA331450.024.6189862
ACATCTC328200.022.7890858
GCAGAGT523300.021.89669
TACATGG347850.020.205332
ATACACA433000.020.0125164
CATCTCC378300.019.9054439
GAGTACT310000.019.70055812-13
TACACAT391200.019.6991355
GTACATG367250.019.526251
Produced by FastQC (version 0.11.5)