##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12800142
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831607961849173	33.0	31.0	34.0	30.0	34.0
2	31.496782770066144	33.0	31.0	34.0	30.0	34.0
3	31.796309134695537	33.0	31.0	34.0	30.0	34.0
4	33.07456378218304	37.0	35.0	37.0	28.0	37.0
5	34.489337540161664	37.0	35.0	37.0	28.0	37.0
6	35.15273463372516	37.0	35.0	37.0	32.0	37.0
7	35.29218324296715	37.0	35.0	37.0	32.0	37.0
8	35.42894008519593	37.0	35.0	37.0	32.0	37.0
9	37.18645683774446	39.0	37.0	39.0	33.0	39.0
10-11	37.06069842818931	39.0	37.0	39.0	33.0	39.0
12-13	36.952655876786366	39.0	37.0	39.0	32.5	39.0
14-15	38.291920355258554	40.0	38.0	41.0	33.0	41.0
16-17	38.285389060527606	40.0	38.0	41.0	33.0	41.0
18-19	38.22663014207186	40.0	38.0	41.0	33.0	41.0
20-21	38.2881502060509	40.0	38.0	41.0	33.0	41.0
22-23	38.32133463668982	40.0	38.0	41.0	33.0	41.0
24-25	38.19648379872959	40.0	38.0	41.0	32.0	41.0
26-27	38.10443340840462	40.0	38.0	41.0	32.0	41.0
28-29	38.11755264889561	40.0	38.0	41.0	32.0	41.0
30-31	38.05197004839482	40.0	37.5	41.0	32.0	41.0
32-33	37.95549330375008	40.0	37.0	41.0	32.0	41.0
34-35	37.838936360704835	40.0	37.0	41.0	31.0	41.0
36-37	37.78884668830064	40.0	37.0	41.0	31.0	41.0
38-39	37.70743881834368	40.0	37.0	41.0	31.0	41.0
40-41	37.63275938308415	40.0	37.0	41.0	31.0	41.0
42-43	37.606754036677444	40.0	37.0	41.0	31.0	41.0
44-45	37.537314286367405	40.0	36.0	41.0	31.0	41.0
46-47	37.39766865068966	40.0	36.0	41.0	30.0	41.0
48-49	37.56040097706732	40.0	36.0	41.0	31.0	41.0
50-51	37.493767992273156	40.0	36.0	41.0	31.0	41.0
52-53	37.4211125305129	39.5	36.0	41.0	30.0	41.0
54-55	37.27341499312567	39.0	35.5	41.0	30.0	41.0
56-57	37.10972312613856	39.0	35.0	41.0	30.0	41.0
58-59	36.97065473456219	39.0	35.0	41.0	30.5	41.0
60-61	36.74071564303948	38.5	35.0	41.0	30.0	41.0
62-63	36.541011993869	38.0	35.0	40.0	30.0	41.0
64-65	36.26850601994663	37.5	35.0	40.0	30.0	41.0
66-67	36.03017916554174	37.0	34.0	40.0	30.0	41.0
68-69	35.765079076800674	37.0	34.0	39.5	30.0	41.0
70-71	35.51335239991581	36.0	34.0	39.0	30.0	41.0
72-73	35.21030690223252	36.0	34.0	39.0	29.0	41.0
74-75	35.04349491018405	35.0	34.0	38.5	29.5	40.0
76-77	34.86444436599386	35.0	34.0	37.0	30.0	39.5
78-79	34.62624003432471	35.0	34.0	37.0	30.0	39.0
80-81	34.39209259289511	35.0	34.0	36.5	30.0	39.0
82-83	34.16195205896726	35.0	34.0	36.0	30.0	38.0
84-85	33.968566764916204	35.0	34.0	36.0	29.0	37.0
86-87	33.80693143380114	35.0	34.0	35.0	29.0	37.0
88-89	33.68963863494271	35.0	34.0	35.0	29.0	36.0
90-91	33.57031166787624	35.0	34.0	35.0	29.0	36.0
92-93	33.53846345512071	35.0	34.0	35.0	29.0	36.0
94-95	33.52592955824477	35.0	34.0	35.0	29.0	36.0
96-97	33.547170740720944	35.0	34.0	35.0	29.5	35.5
98-99	33.58907293722818	35.0	34.0	35.0	30.0	35.0
100-101	33.200889503481164	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	8.0
17	95.0
18	707.0
19	2585.0
20	7231.0
21	15413.0
22	27258.0
23	42989.0
24	66021.0
25	99048.0
26	147879.0
27	205174.0
28	227243.0
29	194501.0
30	233138.0
31	289234.0
32	362859.0
33	478129.0
34	656199.0
35	931534.0
36	1440916.0
37	2508006.0
38	3127636.0
39	1650484.0
40	85853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36675530630832	20.455827755660835	14.281693124966896	24.89572381306395
2	14.412037110585096	20.569185187538725	41.59314184949243	23.425635852383753
3	17.52584109594264	26.436824625254403	30.723556936744036	25.313777342058923
4	12.073413139936774	18.458333610427754	38.34644032571298	31.121812923922494
5	13.139864668339934	37.622749931270114	35.60829052315101	13.629094877238938
6	29.482784258973606	38.0351455958785	18.69130002334513	13.79077012180277
7	25.82188042242829	32.73785620262304	23.05811573291011	18.382147642038564
8	24.26880621897036	36.22424143134943	21.338460808897707	18.1684915407825
9	25.19351202399917	16.851012753094523	21.077359575609865	36.87811564729644
10-11	24.08823260059988	26.58087039167163	28.936322875997533	20.394574131730955
12-13	25.110801788499337	23.943207741649367	28.710879515273273	22.235110954578026
14-15	22.30256994305057	25.191337930370693	26.271608377666222	26.234483748912513
16-17	22.02965814555263	28.43562633680325	26.840134229928676	22.694581287715447
18-19	22.05444451564329	27.30717716161432	28.67395435042983	21.964423972312552
20-21	22.843370750705493	26.530425034838395	28.397975421435255	22.22822879302086
22-23	22.428387678473438	26.908247893966735	28.290347977041165	22.373016450518662
24-25	22.399146602810678	27.01240029158729	28.489019113252596	22.099433992349436
26-27	22.599917160423832	27.085000651162154	28.50671186613281	21.8083703222812
28-29	22.507266823187376	26.987806140551214	28.424156636562163	22.080770399699247
30-31	22.47391716210383	27.204187003378028	28.44829616870331	21.873599665814833
32-33	22.579748827429402	27.22351894473479	28.222699891785684	21.974032336050126
34-35	22.753474686595446	26.936129082327376	28.401770490027683	21.908625741049494
36-37	22.184219435313064	27.22991863317895	28.39791390427945	22.187948027228536
38-39	22.17402517754045	26.73913425222289	28.623740636189044	22.463099934047616
40-41	22.37509816830529	26.64454158568149	28.72984242724322	22.250517818770003
42-43	22.717097120565917	26.5071491771618	28.424588994734584	22.3511647075377
44-45	22.350133465105674	27.107824639728108	28.094826238800767	22.44721565636545
46-47	22.716621285688873	26.671266197569913	27.64149954525775	22.970612971483455
48-49	22.57511038053738	26.577992518819787	27.757683538474865	23.089213562167966
50-51	22.102848183706374	26.795713583849473	28.05570374265206	23.045734489792093
52-53	22.340901582839383	26.900169132667095	28.122554715427718	22.6363745690658
54-55	22.466758192005376	26.76122297848933	27.77464446638543	22.997374363119864
56-57	22.72100867280195	27.078586286943324	27.407092597826427	22.793312442428295
58-59	22.284624044202303	27.51683299921791	27.979933053262574	22.218609903317212
60-61	22.65747534891766	27.986836883127708	26.971662043959572	22.384025723995062
62-63	22.227398929111903	28.32449333202544	26.95138289087754	22.496724847985117
64-65	22.183914845962864	28.18245318051067	27.02480500640346	22.608826967123008
66-67	22.310785459654596	28.101738870909294	26.892449578064415	22.69502609137169
68-69	22.43387248476009	27.739979103262524	27.081759049644795	22.744389362332594
70-71	22.529542908909253	27.18844974213565	27.202202320393358	23.079805028561733
72-73	22.60355972287808	27.150325905297624	27.145054816716485	23.10105955510781
74-75	22.41756389696844	27.279034911042526	27.286580297620706	23.016820894368326
76-77	22.673463920732672	27.237841578277923	27.07881235496781	23.009882146021603
78-79	22.676233962838193	27.29259585493499	26.988514828164067	23.042655354062745
80-81	22.645713297307616	27.227490884220657	27.01941849876932	23.10737731970241
82-83	22.862776381054278	27.162078628035722	27.174626983516337	22.80051800739366
84-85	22.999482127799435	27.130827369048582	26.968601543621233	22.90108895953075
86-87	22.62440014670255	27.304681567651055	27.16778845905501	22.903129826591385
88-89	22.7573624690653	27.214502706511496	27.164633520884873	22.86350130353833
90-91	22.82780537321226	27.20610154777931	27.160032707709075	22.806060371299356
92-93	22.909856773185222	27.19668511232	27.15584392161571	22.737614192879064
94-95	22.573120167590208	27.388065361754364	27.225097044373413	22.813717426282015
96-97	22.578824002373864	27.28214855379773	27.340333290085294	22.798694153743117
98-99	22.92018569700047	27.907868406266	27.22679570061325	21.945150196120277
100-101	23.663313201050908	30.486923618944417	24.241399882737806	21.60836329726687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3019.0
1	2646.0
2	3657.0
3	5174.0
4	6880.0
5	11367.5
6	14934.0
7	16981.0
8	17612.5
9	16017.5
10	13406.0
11	11163.5
12	12332.5
13	17745.5
14	30892.5
15	48878.5
16	63176.5
17	71115.0
18	71687.5
19	65946.0
20	57959.0
21	51182.0
22	48457.5
23	52399.5
24	63275.0
25	79350.5
26	101084.5
27	126105.0
28	149103.5
29	173443.0
30	195993.5
31	218020.5
32	245396.0
33	273747.0
34	302870.0
35	332518.5
36	366589.5
37	396042.5
38	410077.5
39	420744.5
40	439086.0
41	456372.5
42	468471.0
43	487990.5
44	527556.5
45	579133.5
46	590088.0
47	577859.0
48	574021.0
49	562256.0
50	552782.5
51	539394.5
52	514074.5
53	488373.0
54	467060.5
55	438996.0
56	405926.0
57	377452.0
58	346659.0
59	298829.5
60	241054.0
61	188093.5
62	143614.5
63	108657.0
64	82071.5
65	60879.0
66	45169.0
67	34671.5
68	26350.0
69	19886.0
70	15041.5
71	11291.0
72	8259.5
73	5902.0
74	4299.0
75	3221.5
76	2252.5
77	1488.0
78	1026.5
79	716.0
80	503.0
81	383.5
82	292.5
83	199.5
84	140.5
85	111.0
86	91.0
87	68.5
88	46.0
89	31.5
90	21.5
91	15.0
92	11.0
93	6.5
94	3.5
95	2.0
96	2.0
97	2.5
98	2.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6464036102099493
3	0.006710863051363023
4	8.369344652582761
5	2.578096399243071E-4
6	0.07393667976495887
7	0.024820037152712835
8	6.328054798142083E-4
9	2.578096399243071E-4
10-11	0.27570787886571885
12-13	0.2928639385406818
14-15	1.0937378663455453E-4
16-17	1.2499861329663372E-4
18-19	0.4535145000735148
20-21	2.3831751894741484E-4
22-23	1.4068469207520785E-4
24-25	0.16550559425796918
26-27	0.14197279726265777
28-29	1.4873163423619046E-4
30-31	1.0190473670853579E-4
32-33	1.0215559302657633E-4
34-35	3.6655484247719494E-4
36-37	2.7707081839080513E-4
38-39	0.0013856856521926625
40-41	0.001671518948551248
42-43	0.001150654136783304
44-45	8.144754440133245E-4
46-47	0.0013246341891046123
48-49	0.0023844161538960343
50-51	3.059908053535481E-4
52-53	0.0015676619150735425
54-55	5.280116346512064E-4
56-57	0.008674325580848085
58-59	1.9067922670732988E-4
60-61	0.0027310078101571433
62-63	0.001393709353462213
64-65	0.0013972463051903892
66-67	0.00473491189454811
68-69	0.0019253776146846801
70-71	0.002394135154447202
72-73	0.003608647398920579
74-75	7.281843862512273E-4
76-77	3.053491352318618E-4
78-79	7.066238230174271E-4
80-81	4.7218917588326095E-4
82-83	0.0016717433281831556
84-85	2.6518250140228E-4
86-87	4.494036800582593E-4
88-89	1.3083513584899993E-4
90-91	2.8653653945788564E-4
92-93	0.0
94-95	0.0
96-97	2.7794363292006634E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1298.0
20-21	3322.0
22-23	4263.0
24-25	5742.0
26-27	7958.0
28-29	13561.0
30-31	29664.0
32-33	37408.0
34-35	48706.0
36-37	69642.0
38-39	83683.0
40-41	87160.0
42-43	100687.0
44-45	121112.0
46-47	120798.0
48-49	111457.0
50-51	98863.0
52-53	89070.0
54-55	96489.0
56-57	104380.0
58-59	110482.0
60-61	121582.0
62-63	131944.0
64-65	147686.0
66-67	156786.0
68-69	164690.0
70-71	143049.0
72-73	122192.0
74-75	119544.0
76-77	124838.0
78-79	129932.0
80-81	130909.0
82-83	125606.0
84-85	125276.0
86-87	124159.0
88-89	129439.0
90-91	136073.0
92-93	135783.0
94-95	146606.0
96-97	219399.0
98-99	1022158.0
100-101	7796746.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.80616278428622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.73530417903054	70.01817967240578
2	7.557859233045175	12.063274885062475
3	2.2209804535446387	5.317437828489038
4	0.9976765148903597	3.184829374135975
5	0.5188035608076474	2.0701860713441222
6	0.3062315836805742	1.4663500570141001
7	0.19255851852750716	1.0757149532575059
8	0.13086878152519038	0.8355308225424425
9	0.08162295279396886	0.5862613191848629
>10	0.25434336039760164	2.9539061502972044
>50	0.0024980320604615133	0.13323868654113885
>100	0.0011164939504345692	0.16657783996434655
>500	3.8954793235881914E-5	0.019321357773187314
>1k	9.738095264333139E-5	0.1091909819878115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.812413331039609E-6	0.0	0.0	0.0
3	0.0	7.812413331039609E-6	0.0	0.0	0.0
4	0.0	7.812413331039609E-6	0.0	0.0	0.0
5	0.0	1.5624826662079218E-5	0.0	0.0	0.0
6	0.0	1.5624826662079218E-5	0.0	0.0	0.0
7	0.0	1.5624826662079218E-5	0.0	0.0	0.0
8	0.0	3.9062066655198044E-5	0.0	0.0	0.0
9	0.0	5.468689331727726E-5	0.0	0.0	0.0
10-11	0.0	5.468689331727726E-5	0.0	0.0	0.0
12-13	0.0	5.468689331727726E-5	0.0	1.1718619996559414E-5	0.0
14-15	0.0	6.249930664831687E-5	0.0	2.3437239993118826E-5	0.0
16-17	0.0	6.249930664831687E-5	0.0	2.3437239993118826E-5	0.0
18-19	0.0	7.42179266448763E-5	0.0	4.296827332071785E-5	0.0
20-21	0.0	9.765516663799511E-5	0.0	4.687447998623765E-5	0.0
22-23	0.0	1.1327999330007432E-4	0.0	8.59365466414357E-5	0.0
24-25	0.0	1.3281102662767336E-4	0.0	1.79685506613911E-4	0.0
26-27	0.0	1.4062343995871295E-4	0.0	4.3749514653821807E-4	0.0
28-29	0.0	1.4062343995871295E-4	0.0	0.0017304495528252732	0.0
30-31	0.0	1.5624826662079217E-4	0.0	0.007753820231056812	0.0
32-33	0.0	1.5624826662079217E-4	0.0	0.022070067660186897	0.0
34-35	0.0	1.874979199449506E-4	0.0	0.04176125546107223	0.0
36-37	0.0	2.1874757326910903E-4	0.0	0.0723077915854371	0.0
38-39	0.0	2.1874757326910903E-4	0.0	0.12240489207072859	0.0
40-41	0.0	2.499972265932675E-4	0.0	0.19373613198978573	0.0
42-43	0.0	2.499972265932675E-4	0.0	0.2472628819274036	3.9062066655198045E-6
44-45	0.0	2.773406732519061E-4	0.0	0.3116840422551562	7.812413331039609E-6
46-47	0.0	3.0468411991054476E-4	0.0	0.3835269952473965	7.812413331039609E-6
48-49	0.0	3.0859032657606456E-4	0.0	0.451479366400779	7.812413331039609E-6
50-51	0.0	3.710896332243814E-4	0.0	0.519037210680944	7.812413331039609E-6
52-53	0.0	3.749958398899012E-4	0.0	0.5923371787594232	7.812413331039609E-6
54-55	0.0	3.749958398899012E-4	0.0	0.6973828884085818	7.812413331039609E-6
56-57	0.0	3.8280825322094085E-4	0.0	0.8120417726615845	7.812413331039609E-6
58-59	0.0	3.8280825322094085E-4	0.0	0.9095289724129623	7.812413331039609E-6
60-61	0.0	4.023392865485398E-4	0.0	0.986996863003551	7.812413331039609E-6
62-63	0.0	4.179641132106191E-4	0.0	1.0207933630736283	7.812413331039609E-6
64-65	0.0	4.921820398554954E-4	0.0	1.0304377873307968	7.812413331039609E-6
66-67	0.0	4.99994453186535E-4	0.0	1.036015850449159	7.812413331039609E-6
68-69	0.0	5.117130731830944E-4	0.0	1.041953284580749	7.812413331039609E-6
70-71	0.0	5.19525486514134E-4	0.0	1.047203226339208	7.812413331039609E-6
72-73	0.0	5.273378998451735E-4	0.0	1.051601615044583	7.812413331039609E-6
74-75	0.0	5.39056519841733E-4	0.0	1.055328136203489	7.812413331039609E-6
76-77	0.0	5.703061731658914E-4	0.0	1.0586757553158395	7.812413331039609E-6
78-79	0.0	5.781185864969311E-4	0.0	1.0614647868750207	7.812413331039609E-6
80-81	0.0	5.781185864969311E-4	0.0	1.0628632088612768	7.812413331039609E-6
82-83	0.0	5.820247931624509E-4	0.0	1.063316328834477	1.1718619996559414E-5
84-85	0.0	5.898372064934904E-4	0.0	1.063691324674367	1.5624826662079218E-5
86-87	0.0	6.015558264900498E-4	0.0	1.0637928860476704	1.5624826662079218E-5
88-89	0.0	6.171806531521291E-4	0.0	1.0638631977676498	1.5624826662079218E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15025	0.0	54.98275	1
GTATCAA	28915	0.0	42.553486	1
ATCAACG	35545	0.0	33.952393	3
TCAACGC	36335	0.0	33.28721	4
TATCAAC	36780	0.0	33.016174	2
CAACGCA	37365	0.0	31.004423	5
AACGCAG	39540	0.0	29.563253	6
CTTATAC	27830	0.0	28.593512	1
ACGCAGA	43355	0.0	26.84622	7
TATACAC	32190	0.0	26.138344	3
CGCAGAG	45330	0.0	25.707968	8
TTATACA	33145	0.0	24.618986	2
ACATCTC	32820	0.0	22.789085	8
GCAGAGT	52330	0.0	21.8966	9
TACATGG	34785	0.0	20.20533	2
ATACACA	43300	0.0	20.012516	4
CATCTCC	37830	0.0	19.905443	9
GAGTACT	31000	0.0	19.700558	12-13
TACACAT	39120	0.0	19.699135	5
GTACATG	36725	0.0	19.52625	1
>>END_MODULE