FastQCFastQC Report
Fri 27 May 2016
SRR2139973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772887
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA332500.032.333171
GTATCAA489200.028.9652561
CTTATAC344050.025.4675641
ATCAACG560250.024.4859143
TCAACGC574950.023.9064164
TATACAC387350.023.8476923
TATCAAC591500.023.7684962
CAACGCA583500.022.4972115
TTATACA413950.021.847022
AACGCAG607700.021.7749866
ACATCTC375500.021.492038
TACATGG407150.021.13532
GTACATG431250.020.3393481
ACGCAGA666500.019.8077587
CGCAGAG670000.019.7252148
ACATGGG423200.019.677343
GAGTACT379350.019.16938212-13
CATCTCC423000.019.0384779
TATGCCG243550.018.64341742-43
CCGTCTT251000.018.4411746-47
Produced by FastQC (version 0.11.5)