##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14772887
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.826363391258596	33.0	31.0	34.0	30.0	34.0
2	31.46291242869454	33.0	31.0	34.0	29.0	34.0
3	31.777672908484305	33.0	31.0	34.0	30.0	34.0
4	33.00206818071511	37.0	35.0	37.0	28.0	37.0
5	34.45871676944392	37.0	35.0	37.0	28.0	37.0
6	35.15112767057651	37.0	35.0	37.0	32.0	37.0
7	35.290362134361416	37.0	35.0	37.0	32.0	37.0
8	35.4309977460736	37.0	35.0	37.0	32.0	37.0
9	37.19008044940708	39.0	37.0	39.0	33.0	39.0
10-11	37.06194222564621	39.0	37.0	39.0	33.0	39.0
12-13	36.954367484162034	39.0	37.0	39.0	32.5	39.0
14-15	38.28963299455279	40.0	38.0	41.0	33.0	41.0
16-17	38.286639774608716	40.0	38.0	41.0	33.0	41.0
18-19	38.23062509717972	40.0	38.0	41.0	33.0	41.0
20-21	38.29780976850368	40.0	38.0	41.0	33.0	41.0
22-23	38.33431344737538	40.0	38.0	41.0	33.0	41.0
24-25	38.21122369670628	40.0	38.0	41.0	32.0	41.0
26-27	38.11579534284776	40.0	38.0	41.0	32.0	41.0
28-29	38.13086891474461	40.0	38.0	41.0	32.0	41.0
30-31	38.0758627187478	40.0	37.5	41.0	32.0	41.0
32-33	37.96730884248278	40.0	37.0	41.0	32.0	41.0
34-35	37.84587502206363	40.0	37.0	41.0	31.0	41.0
36-37	37.79623181037225	40.0	37.0	41.0	31.0	41.0
38-39	37.7015343327833	40.0	37.0	41.0	31.0	41.0
40-41	37.64286931419058	40.0	37.0	41.0	31.0	41.0
42-43	37.623554202874345	40.0	37.0	41.0	31.0	41.0
44-45	37.556698529139915	40.0	36.5	41.0	31.0	41.0
46-47	37.416594589827774	40.0	36.0	41.0	30.0	41.0
48-49	37.58696019885646	40.0	36.5	41.0	31.0	41.0
50-51	37.53028623081781	40.0	36.0	41.0	31.0	41.0
52-53	37.464396198608654	40.0	36.0	41.0	30.0	41.0
54-55	37.33142642792183	39.0	36.0	41.0	30.0	41.0
56-57	37.18015125543856	39.0	35.0	41.0	30.0	41.0
58-59	37.04962894373331	39.0	35.0	41.0	30.5	41.0
60-61	36.82546543862263	39.0	35.0	41.0	30.0	41.0
62-63	36.64458252263086	38.0	35.0	40.5	30.0	41.0
64-65	36.37884448854446	38.0	35.0	40.0	30.0	41.0
66-67	36.153965568577036	37.0	34.5	40.0	30.0	41.0
68-69	35.893063513948626	37.0	34.0	40.0	30.0	41.0
70-71	35.645456200568496	36.5	34.0	39.0	30.0	41.0
72-73	35.339650095482874	36.0	34.0	39.0	30.0	41.0
74-75	35.17376169421911	36.0	34.0	39.0	30.0	40.0
76-77	34.981200202829086	35.0	34.0	37.5	30.0	39.5
78-79	34.73287230395725	35.0	34.0	37.0	30.0	39.0
80-81	34.49180465499372	35.0	34.0	37.0	30.0	39.0
82-83	34.25012814106242	35.0	34.0	36.0	30.0	38.5
84-85	34.044298330298076	35.0	34.0	36.0	30.0	37.0
86-87	33.87338498460676	35.0	34.0	35.5	29.0	37.0
88-89	33.742352572854834	35.0	34.0	35.0	29.0	36.5
90-91	33.616352278545946	35.0	34.0	35.0	29.0	36.0
92-93	33.578000911813604	35.0	34.0	35.0	29.0	36.0
94-95	33.5579079017306	35.0	34.0	35.0	29.0	36.0
96-97	33.57226205785177	35.0	34.0	35.0	30.0	36.0
98-99	33.60994501557602	35.0	34.0	35.0	30.0	35.0
100-101	33.22241324754071	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	10.0
17	121.0
18	695.0
19	2734.0
20	7292.0
21	16341.0
22	29232.0
23	46201.0
24	73250.0
25	111941.0
26	168959.0
27	238494.0
28	267558.0
29	224687.0
30	266890.0
31	331174.0
32	414353.0
33	545449.0
34	748312.0
35	1061056.0
36	1625648.0
37	2807993.0
38	3662901.0
39	2011598.0
40	109994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29973288227278	20.651210558911064	14.31587475081885	24.733181807997312
2	14.11397091196821	20.958111145148155	41.918633821882075	23.00928412100156
3	17.557052748101402	26.788173580945067	31.034209173823022	24.620564497130506
4	11.780430841952438	18.670407519715557	39.07604008340175	30.473121554930255
5	12.772622931921443	38.059739531305816	35.87051121074897	13.297126326023767
6	28.66056218735916	38.70152087661607	18.866467953041695	13.77144898298307
7	25.27200249492761	33.05823091161681	23.53177559247421	18.13799100098137
8	23.77908241245754	36.549732640447445	21.535797911879012	18.135387035215995
9	24.922866932826153	17.04490001148729	21.516854537246612	36.515378518439945
10-11	23.88503939723192	26.849233581047294	29.10544925009018	20.1602777716306
12-13	25.0158471495957	24.20963094582965	28.95635168997846	21.81817021459619
14-15	22.04902038696059	25.54081893611223	26.728620984650654	25.681539692276534
16-17	21.58378839047524	28.840008745761658	27.320686501675027	22.25551636208807
18-19	21.520326920639032	27.986523439731574	28.94173201506663	21.551417624562767
20-21	22.418874722220774	27.053856756743766	28.64160330685831	21.885665214177152
22-23	21.98160313737006	27.283667924306982	28.63529800027639	22.09943093804657
24-25	21.986927770122257	27.407640817686072	28.831692486849263	21.773738925342403
26-27	22.035464452460136	27.59951282097155	28.843919358474963	21.521103368093346
28-29	21.751731095208584	27.830052454140166	28.720770139500495	21.69744631115076
30-31	22.34711608658285	27.35244497503014	28.643682694834045	21.65675624355297
32-33	22.01478273123392	27.510831956397425	28.743539394904126	21.730845917464524
34-35	22.090180693249874	27.137450713605393	28.849288698643647	21.923079894501086
36-37	21.8831786686194	27.42237772144187	28.935773575294743	21.758670034643984
38-39	21.810200619363464	27.096673060466642	29.14653944974816	21.94658687042173
40-41	21.998508956828868	27.036314918998848	29.077706143915638	21.887469980256647
42-43	22.13499699682852	26.985174093067954	28.82911892996813	22.050709980135398
44-45	21.834439614706266	27.410932094881286	28.558651257865712	22.19597703254674
46-47	22.439140961683638	26.918044746075083	28.102939209687833	22.53987508255345
48-49	22.282701123714695	26.993772786180593	28.205364163809143	22.51816192629557
50-51	21.746764330287697	27.245257991274606	28.423638543782133	22.58433913465557
52-53	21.92906400207875	27.32531698211981	28.484793385346798	22.260825630454644
54-55	21.954422986467375	27.161792649939876	28.190263396296267	22.69352096729648
56-57	22.333358273709084	27.460943185451796	27.681929804381838	22.523768736457285
58-59	21.948629024576324	27.814251167156804	28.269826941978554	21.967292866288314
60-61	22.22696743104406	28.220195258872472	27.552242517105608	22.00059479297786
62-63	21.84709925112312	28.58724427734986	27.495718421771475	22.069938049755546
64-65	21.766607880570387	28.548988907210692	27.44297709212494	22.241426120093976
66-67	21.92741582570789	28.4553169373782	27.197007800381822	22.420259436532092
68-69	22.028865839247718	28.102487015182525	27.447515595322088	22.421131550247665
70-71	22.243539627908927	27.491835254166652	27.558519780013118	22.706105337911307
72-73	22.235370522972513	27.4618125568572	27.746658553895042	22.556158366275245
74-75	22.05275961672682	27.656165380202175	27.654038581455914	22.637036421615093
76-77	22.28575961097266	27.62915156431435	27.429075063859088	22.656013760853906
78-79	22.31283047100547	27.575828496277722	27.47004208897928	22.641298943737528
80-81	22.217470126037732	27.485999502493407	27.520637290533823	22.77589308093504
82-83	22.308520912961534	27.494994850843657	27.644426564248377	22.552057671946436
84-85	22.593609726503473	27.530777533647978	27.460752014983527	22.414860724865022
86-87	22.132180869216118	27.74425776905592	27.56778945923991	22.555771902488054
88-89	22.312339261841345	27.642830555867974	27.559038490696956	22.48579169159372
90-91	22.35807972628566	27.658101529511203	27.646356736151333	22.3374620080518
92-93	22.40338220552126	27.65015294158012	27.554140237844482	22.392324615054136
94-95	22.254217905522893	27.630719704156377	27.610351120319425	22.50471127000131
96-97	22.074680392427762	27.726752362140967	27.806488419322477	22.392078826108797
98-99	22.370391402852942	28.2906841510493	27.77728857288249	21.561635873215263
100-101	23.132292826455807	30.7293388609148	24.888242802155194	21.250125510474195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4044.0
1	3520.5
2	4565.0
3	6355.0
4	8207.0
5	13096.0
6	17271.5
7	20138.5
8	21552.0
9	20128.0
10	16841.0
11	13801.0
12	14950.0
13	21036.5
14	36131.0
15	57232.0
16	74327.0
17	83744.5
18	84867.0
19	79522.5
20	71344.5
21	63795.5
22	61142.0
23	66912.0
24	80556.0
25	101076.5
26	129470.5
27	161078.5
28	190976.5
29	219535.5
30	246627.5
31	274439.0
32	308137.0
33	344660.0
34	380214.5
35	415054.5
36	452850.5
37	484559.0
38	498998.0
39	508584.5
40	527808.0
41	543984.5
42	555027.5
43	577399.5
44	616589.5
45	665210.5
46	686258.0
47	700676.5
48	700020.0
49	663204.0
50	627977.5
51	600497.0
52	570307.5
53	534571.5
54	497174.0
55	459856.5
56	425682.5
57	387364.5
58	339936.0
59	291983.0
60	242699.5
61	190630.5
62	148587.0
63	113685.0
64	82736.5
65	59682.0
66	43394.5
67	32785.5
68	25455.0
69	19621.5
70	15153.5
71	11068.5
72	8040.5
73	5711.5
74	3830.5
75	2621.0
76	1790.5
77	1174.0
78	809.0
79	585.0
80	423.0
81	307.0
82	243.0
83	167.5
84	102.0
85	62.5
86	44.0
87	38.0
88	30.5
89	22.5
90	15.5
91	12.5
92	10.0
93	5.5
94	2.0
95	1.0
96	1.0
97	0.5
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.7551680995055334
3	0.006383315597012283
4	8.532259131204349
5	3.7907282442490757E-4
6	0.0740545839144373
7	0.025188035351519308
8	5.686092366373614E-4
9	2.707663031606483E-4
10-11	0.281928644008446
12-13	0.29714909482486396
14-15	8.799904852721069E-5
16-17	1.1507567884327553E-4
18-19	0.4679857092252855
20-21	2.0988058201104053E-4
22-23	1.3544963912999206E-4
24-25	0.16856368993332724
26-27	0.1546949872709403
28-29	1.0518980720269222E-4
30-31	9.8544581483711E-5
32-33	1.0562100328845884E-4
34-35	3.5209937543382143E-4
36-37	2.128700329224106E-4
38-39	0.0014093358204426026
40-41	0.001780152303155487
42-43	0.0011486548079030674
44-45	8.301147840334522E-4
46-47	0.0013452445160322282
48-49	0.0025005082084766654
50-51	3.417206857999714E-4
52-53	0.001637967425335069
54-55	4.5039509063444393E-4
56-57	0.00936479238560493
58-59	1.0892550213060159E-4
60-61	0.0027428468413292313
62-63	0.0014687479579418078
64-65	0.0013347658267820841
66-67	0.004950368525250222
68-69	0.0020540599871667117
70-71	0.0025177085581528343
72-73	0.0036272528416554537
74-75	6.011383321288456E-4
76-77	3.7331478783765587E-4
78-79	7.986701631993265E-4
80-81	4.7778999657540787E-4
82-83	0.0016002965127053512
84-85	2.4288290169288056E-4
86-87	4.875162082366892E-4
88-89	1.0422451410418236E-4
90-91	2.986734304711229E-4
92-93	0.0
94-95	0.0
96-97	6.26022523519907E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1563.0
20-21	4461.0
22-23	5533.0
24-25	7754.0
26-27	14816.0
28-29	16480.0
30-31	36685.0
32-33	45832.0
34-35	57730.0
36-37	82127.0
38-39	94873.0
40-41	100327.0
42-43	116189.0
44-45	140244.0
46-47	138630.0
48-49	126295.0
50-51	113752.0
52-53	98770.0
54-55	108285.0
56-57	119317.0
58-59	129089.0
60-61	140090.0
62-63	147619.0
64-65	166213.0
66-67	176675.0
68-69	186754.0
70-71	163017.0
72-73	139426.0
74-75	138669.0
76-77	147347.0
78-79	154330.0
80-81	151242.0
82-83	144774.0
84-85	143435.0
86-87	143515.0
88-89	150842.0
90-91	156085.0
92-93	155651.0
94-95	175732.0
96-97	249090.0
98-99	1176673.0
100-101	9006956.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.74521155579632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.03897813717082	71.08725914841983
2	7.464822075903903	12.054972754904783
3	2.221283768409151	5.3807408341696
4	0.953507918806361	3.079647944965867
5	0.48221839516743054	1.9468413166945382
6	0.2594359749552482	1.2568927609767475
7	0.17530486882660162	0.9908520104115548
8	0.10587403648499917	0.6839057179397884
9	0.0748873286690233	0.5442113876605863
>10	0.220470723711565	2.6309088730696244
>50	0.0022395503251147683	0.1196472407455057
>100	8.642682943977758E-4	0.12130815931311412
>500	6.454655058116379E-5	0.040117736028966344
>1k	4.840672484005525E-5	0.06269411469948474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	6.769157579016207E-6	6.769157579016207E-6	0.0
6	0.0	0.0	6.769157579016207E-6	6.769157579016207E-6	0.0
7	0.0	0.0	6.769157579016207E-6	6.769157579016207E-6	0.0
8	0.0	0.0	6.769157579016207E-6	6.769157579016207E-6	0.0
9	0.0	0.0	6.769157579016207E-6	2.030747273704862E-5	0.0
10-11	0.0	0.0	6.769157579016207E-6	3.384578789508104E-5	0.0
12-13	0.0	3.3845787895081035E-6	6.769157579016207E-6	5.0768681842621554E-5	0.0
14-15	0.0	1.3538315158032414E-5	6.769157579016207E-6	9.13836273167188E-5	0.0
16-17	0.0	2.030747273704862E-5	6.769157579016207E-6	1.4553688794884847E-4	0.0
18-19	0.0	2.3692051526556726E-5	6.769157579016207E-6	2.0984388494950243E-4	0.0
20-21	0.0	2.7076630316064828E-5	6.769157579016207E-6	3.3507330016130225E-4	0.0
22-23	0.0	2.7076630316064828E-5	6.769157579016207E-6	5.347634487422804E-4	0.0
24-25	0.0	3.0461209105572933E-5	6.769157579016207E-6	7.81837700376372E-4	0.0
26-27	0.0	3.384578789508104E-5	6.769157579016207E-6	0.0011609105248012795	0.0
28-29	0.0	3.384578789508104E-5	6.769157579016207E-6	0.003208580692453682	0.0
30-31	0.0	3.384578789508104E-5	6.769157579016207E-6	0.010113121423050214	0.0
32-33	0.0	3.384578789508104E-5	6.769157579016207E-6	0.02719847515248712	0.0
34-35	0.0	4.061494547409724E-5	6.769157579016207E-6	0.04945884985108191	0.0
36-37	0.0	5.0768681842621554E-5	6.769157579016207E-6	0.08128065963003711	0.0
38-39	0.0	6.0922418211145866E-5	6.769157579016207E-6	0.13572499403806446	0.0
40-41	0.0	6.0922418211145866E-5	6.769157579016207E-6	0.2219031391765198	0.0
42-43	0.0	6.0922418211145866E-5	6.769157579016207E-6	0.2782969909673038	0.0
44-45	0.0	6.430699700065397E-5	6.769157579016207E-6	0.34682117313968486	0.0
46-47	0.0	7.446073336917828E-5	6.769157579016207E-6	0.4231840397885667	0.0
48-49	0.0	7.446073336917828E-5	6.769157579016207E-6	0.49411465748028804	0.0
50-51	0.0	7.446073336917828E-5	6.769157579016207E-6	0.563650828710732	0.0
52-53	0.0	7.446073336917828E-5	6.769157579016207E-6	0.6460923988655705	3.3845787895081035E-6
54-55	0.0	7.446073336917828E-5	6.769157579016207E-6	0.7847924376596125	6.769157579016207E-6
56-57	0.0	7.446073336917828E-5	6.769157579016207E-6	0.934312974843712	6.769157579016207E-6
58-59	0.0	7.446073336917828E-5	6.769157579016207E-6	1.0390183042759347	6.769157579016207E-6
60-61	0.0	7.446073336917828E-5	6.769157579016207E-6	1.1180719110624753	6.769157579016207E-6
62-63	0.0	7.446073336917828E-5	6.769157579016207E-6	1.1488648088894202	6.769157579016207E-6
64-65	0.0	7.446073336917828E-5	1.015373636852431E-5	1.1589373153669964	6.769157579016207E-6
66-67	0.0	7.446073336917828E-5	1.3538315158032414E-5	1.164616638575791	6.769157579016207E-6
68-69	0.0	7.446073336917828E-5	1.3538315158032414E-5	1.170258731417901	6.769157579016207E-6
70-71	0.0	7.784531215868638E-5	1.3538315158032414E-5	1.175755287372062	6.769157579016207E-6
72-73	3.3845787895081035E-6	8.122989094819448E-5	1.3538315158032414E-5	1.180151855219633	6.769157579016207E-6
74-75	6.769157579016207E-6	8.122989094819448E-5	1.3538315158032414E-5	1.1835262802727726	6.769157579016207E-6
76-77	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.1866840922833837	6.769157579016207E-6
78-79	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.1895372922029392	6.769157579016207E-6
80-81	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.19090804661269	6.769157579016207E-6
82-83	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.1915409628463278	6.769157579016207E-6
84-85	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.191828652043436	6.769157579016207E-6
86-87	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.1919234202495423	6.769157579016207E-6
88-89	6.769157579016207E-6	8.799904852721069E-5	1.3538315158032414E-5	1.1919471123010688	6.769157579016207E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	33250	0.0	32.33317	1
GTATCAA	48920	0.0	28.965256	1
CTTATAC	34405	0.0	25.467564	1
ATCAACG	56025	0.0	24.485914	3
TCAACGC	57495	0.0	23.906416	4
TATACAC	38735	0.0	23.847692	3
TATCAAC	59150	0.0	23.768496	2
CAACGCA	58350	0.0	22.497211	5
TTATACA	41395	0.0	21.84702	2
AACGCAG	60770	0.0	21.774986	6
ACATCTC	37550	0.0	21.49203	8
TACATGG	40715	0.0	21.1353	2
GTACATG	43125	0.0	20.339348	1
ACGCAGA	66650	0.0	19.807758	7
CGCAGAG	67000	0.0	19.725214	8
ACATGGG	42320	0.0	19.67734	3
GAGTACT	37935	0.0	19.169382	12-13
CATCTCC	42300	0.0	19.038477	9
TATGCCG	24355	0.0	18.643417	42-43
CCGTCTT	25100	0.0	18.44117	46-47
>>END_MODULE