FastQCFastQC Report
Fri 27 May 2016
SRR2139972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9640740
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138050.041.835781
GTATCAA230800.036.574381
ATCAACG272050.030.4290223
TCAACGC279550.029.7101214
TATCAAC286000.029.2729452
CAACGCA280800.028.1160435
AACGCAG292750.027.1887046
ACGCAGA326900.024.2373287
CGCAGAG330350.023.9013758
GCAGAGT376450.020.7022849
CTTATAC235350.020.3385411
TACATGG226300.019.3552152
GAGTACT231550.019.1513312-13
AGAGTAC324750.019.14775810-11
GTACATG237350.018.940781
TATACAC272850.018.8484753
ACATGGG229550.018.372373
CAGAGTA365950.017.84686710-11
ACATCTC257350.017.659948
AGTACTT251850.017.2443612-13
Produced by FastQC (version 0.11.5)