##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9640740
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81611121138004	33.0	31.0	34.0	30.0	34.0
2	31.46797009358203	33.0	31.0	34.0	28.0	34.0
3	31.77278549157015	33.0	31.0	34.0	30.0	34.0
4	33.10848316622998	37.0	35.0	37.0	28.0	37.0
5	34.49843175938776	37.0	35.0	37.0	30.0	37.0
6	35.14780514773762	37.0	35.0	37.0	32.0	37.0
7	35.27376581050832	37.0	35.0	37.0	32.0	37.0
8	35.41517093086215	37.0	35.0	37.0	32.0	37.0
9	37.17276879160728	39.0	37.0	39.0	33.0	39.0
10-11	37.04792645585297	39.0	37.0	39.0	33.0	39.0
12-13	36.94534221439433	39.0	37.0	39.0	32.5	39.0
14-15	38.2688678981074	40.0	38.0	41.0	33.0	41.0
16-17	38.2647739177698	40.0	38.0	41.0	32.5	41.0
18-19	38.211921595230244	40.0	38.0	41.0	33.0	41.0
20-21	38.280855482440316	40.0	38.0	41.0	32.5	41.0
22-23	38.317674148802645	40.0	38.0	41.0	33.0	41.0
24-25	38.20498053031383	40.0	38.0	41.0	32.0	41.0
26-27	38.111497275229404	40.0	38.0	41.0	32.0	41.0
28-29	38.1070361205887	40.0	38.0	41.0	32.0	41.0
30-31	38.05031015401019	40.0	37.5	41.0	32.0	41.0
32-33	37.95172366212428	40.0	37.0	41.0	32.0	41.0
34-35	37.833599888957565	40.0	37.0	41.0	31.0	41.0
36-37	37.78443785032971	40.0	37.0	41.0	31.0	41.0
38-39	37.698318084204494	40.0	37.0	41.0	31.0	41.0
40-41	37.63867414718172	40.0	37.0	41.0	31.0	41.0
42-43	37.62108008624688	40.0	37.0	41.0	31.0	41.0
44-45	37.55511942199164	40.0	36.5	41.0	31.0	41.0
46-47	37.424338048863454	40.0	36.0	41.0	30.0	41.0
48-49	37.58887455488178	40.0	36.5	41.0	31.0	41.0
50-51	37.54045503073608	40.0	36.0	41.0	30.5	41.0
52-53	37.49178711476463	40.0	36.0	41.0	31.0	41.0
54-55	37.375380718008756	39.5	36.0	41.0	30.0	41.0
56-57	37.24810658823263	39.0	35.0	41.0	30.0	41.0
58-59	37.144625212167014	39.0	35.0	41.0	30.5	41.0
60-61	36.95109428811878	39.0	35.0	41.0	30.5	41.0
62-63	36.7926306967898	39.0	35.0	41.0	30.0	41.0
64-65	36.557060815917225	38.0	35.0	40.0	30.0	41.0
66-67	36.359474122294785	38.0	35.0	40.0	30.0	41.0
68-69	36.1238229849754	37.0	34.5	40.0	30.0	41.0
70-71	35.88983692770492	37.0	34.0	39.5	30.0	41.0
72-73	35.59479334148075	36.0	34.0	39.0	30.0	41.0
74-75	35.423067090739906	36.0	34.0	39.0	30.0	41.0
76-77	35.21608079175027	36.0	34.0	39.0	30.0	40.0
78-79	34.94552189389263	35.0	34.0	37.5	30.0	39.0
80-81	34.67797545031081	35.0	34.0	37.0	30.0	39.0
82-83	34.404505779577825	35.0	34.0	36.5	30.0	39.0
84-85	34.17500781147836	35.0	34.0	36.0	30.0	37.5
86-87	33.977944426696936	35.0	34.0	36.0	29.5	37.0
88-89	33.82494295674353	35.0	34.0	35.5	29.0	37.0
90-91	33.67497488822446	35.0	34.0	35.0	29.0	36.0
92-93	33.62576335281905	35.0	34.0	35.0	29.0	36.0
94-95	33.59519433116337	35.0	34.0	35.0	29.0	36.0
96-97	33.59614766373322	35.0	34.0	35.0	30.0	36.0
98-99	33.63042747705456	35.0	34.0	35.0	30.0	36.0
100-101	33.243422321628906	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	6.0
17	72.0
18	516.0
19	1769.0
20	5018.0
21	10785.0
22	19189.0
23	30500.0
24	47581.0
25	72810.0
26	108746.0
27	153464.0
28	173357.0
29	147417.0
30	175353.0
31	215778.0
32	269728.0
33	353264.0
34	483433.0
35	677262.0
36	1031002.0
37	1770837.0
38	2412828.0
39	1410775.0
40	69248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.341896991309795	21.261230984343527	14.70874642402969	24.688125600316987
2	13.644319315621143	21.276474329094782	42.49852036256417	22.5806859927199
3	16.888697836333264	27.134997613629565	31.619869912987987	24.356434637049187
4	11.422494998057294	19.18303353688332	39.80122834991195	29.593243115147438
5	12.38010873674826	38.125533220305755	36.448468811701204	13.045889231244779
6	27.937774259477997	39.54091793368538	19.365271453886518	13.156036352950103
7	24.642514353325083	33.48173688072835	24.143041362123988	17.73270740382258
8	23.240136703879763	37.38078137959391	22.038086062701723	17.3409958538246
9	24.605832972882702	17.1207411072421	21.971047775526905	36.30237814434829
10-11	23.117328513472444	27.336192369528955	29.720155529128323	19.826323587870277
12-13	24.200840519707484	24.834751536965182	29.572598849486635	21.391809093840696
14-15	21.39124319423944	26.261527913342665	27.241557433928758	25.105671458489137
16-17	21.003179636056455	29.44779262474631	27.859224502387057	21.689803236810175
18-19	20.86803054596417	28.37284519531469	29.670717840577993	21.088406418143148
20-21	21.673922105202443	27.66252220359229	29.443265347789023	21.220290343416252
22-23	21.419029666629825	27.84513393489499	29.31686899456602	21.418967403909164
24-25	21.354211832397212	27.79418214441014	29.60658218192564	21.245023841267006
26-27	21.47186845519295	27.986246937837006	29.440369707672026	21.10151489929802
28-29	21.013691847273762	27.88822374748814	29.443837691732526	21.65424671350557
30-31	21.669106523328363	27.786526964600633	29.456751209915836	21.087615302155168
32-33	21.71387793770436	27.702414712717342	29.537018898069178	21.046688451509116
34-35	21.129352079579387	27.842862564641514	29.86628743819642	21.161497917582675
36-37	21.488635769136465	27.767542192636725	29.490356103815934	21.253465934410876
38-39	21.15361380430118	27.735034650276468	29.705068634104947	21.406282911317405
40-41	21.347547522332263	27.596763655524676	29.650030817695296	21.405658004447766
42-43	21.494207469302655	27.59558283327999	29.54478700077965	21.365422696637708
44-45	21.269947988542782	28.043456393477427	29.186440683456972	21.50015493452282
46-47	21.65276507338858	27.636801847426458	28.697507092116403	22.01292598706856
48-49	21.611343813240605	27.659681859879154	28.863790157905356	21.86518416897488
50-51	21.29170478498051	27.997443024062648	28.825501049250658	21.885351141706188
52-53	21.316450418732806	28.170327193888216	28.87579048097318	21.637431906405798
54-55	21.37770285864716	28.09970014316676	28.59057371285147	21.932023285334605
56-57	21.65281129766124	28.33595676849965	28.242341226888414	21.768890706950693
58-59	21.272933636764897	28.61458076572523	28.741420128518936	21.37106546899094
60-61	21.462190232594597	28.789123767744094	28.16717919154016	21.581506808121144
62-63	21.339573130490344	29.003028902022727	28.16852632127874	21.488871646208192
64-65	21.088154334156897	28.92326805163119	28.2451564277006	21.74342118651131
66-67	21.25454869656681	28.902850028836724	28.015133877295877	21.827467397300587
68-69	21.358366850858012	28.79078983981107	28.17427551837887	21.67656779095205
70-71	21.422507507350026	28.265179739382905	28.345032832753375	21.967279920513693
72-73	21.475374477816533	28.246552836541248	28.26233665716306	22.015736028479154
74-75	21.509321417099617	28.321504885082298	28.12912357162726	22.04005012619082
76-77	21.506754149075192	28.155332166512025	28.23622534702908	22.101688337383703
78-79	21.574384446259153	28.13563721638264	28.19882313260861	22.091155204749597
80-81	21.685674103466095	28.07593976808795	28.053470996170166	22.184915132275794
82-83	21.83225290197178	28.21113045623652	28.193383466823036	21.76323317496866
84-85	21.866720414703863	28.08112177928213	28.01414550615326	22.038012299860743
86-87	21.531425138073494	28.41315525765209	28.05897296104723	21.996446643227184
88-89	21.863402194781475	28.11838936416643	28.016953558057807	22.001254882994285
90-91	21.985729716256074	28.306856106241256	28.104080982648554	21.603333194854113
92-93	21.745867366022082	28.35521697798501	28.063489660594914	21.835425995398
94-95	21.703058793594384	28.177727603738557	28.179301874372204	21.93991172829485
96-97	21.64571844480088	28.43896323632154	28.31603155586916	21.599286763008422
98-99	21.978706092775667	28.845924616473003	28.354334117743317	20.821035173008013
100-101	22.485163108883977	31.477889117744738	25.56216390118059	20.47478387219069
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2110.0
1	1888.5
2	2626.0
3	3701.0
4	4963.0
5	8250.5
6	10961.0
7	12562.5
8	13040.5
9	11873.5
10	9978.0
11	8353.5
12	9297.0
13	13570.0
14	23657.0
15	37663.0
16	49334.0
17	56155.5
18	57285.5
19	53894.0
20	49027.0
21	44575.5
22	43270.5
23	48094.5
24	58418.0
25	73167.5
26	92779.0
27	115294.0
28	138037.5
29	161613.0
30	183233.5
31	203950.5
32	229600.5
33	257241.5
34	284923.0
35	311510.0
36	336534.5
37	358997.5
38	371556.5
39	377431.5
40	386593.5
41	392943.5
42	394158.0
43	397453.5
44	403369.5
45	409732.0
46	416131.0
47	427802.5
48	438250.0
49	424813.0
50	398573.0
51	374037.5
52	341035.5
53	308009.0
54	280524.0
55	253283.0
56	225722.5
57	197565.0
58	171526.0
59	147472.0
60	121614.5
61	95751.5
62	73343.5
63	55857.0
64	41579.5
65	30341.5
66	22585.5
67	17449.0
68	13446.0
69	9963.5
70	7301.5
71	5387.0
72	3910.0
73	2776.5
74	1951.0
75	1316.0
76	949.5
77	661.0
78	450.0
79	336.5
80	246.5
81	166.5
82	119.0
83	90.0
84	68.5
85	52.0
86	39.0
87	27.5
88	14.0
89	6.0
90	3.0
91	2.0
92	2.5
93	3.5
94	3.5
95	3.0
96	3.0
97	2.5
98	1.0
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6635341270483388
3	0.005985017747600288
4	8.191062096892978
5	2.593161935702031E-4
6	0.0757099558747565
7	0.02469727427562614
8	4.9788709165479E-4
9	3.1117943228424374E-4
10-11	0.27049790783695027
12-13	0.28669998361121657
14-15	1.0372647742808126E-4
16-17	1.4521706839931375E-4
18-19	0.45356995417364226
20-21	2.1267266621445197E-4
22-23	1.4527947048538598E-4
24-25	0.1633639032938012
26-27	0.14268622117532692
28-29	1.0393545358887043E-4
30-31	1.7692029943448467E-4
32-33	7.301943391370918E-5
34-35	4.2895808122237573E-4
36-37	2.5212462267974436E-4
38-39	0.0012943134628569544
40-41	0.0016906514999986443
42-43	0.0010493701904981671
44-45	8.260946564094145E-4
46-47	0.0012814039388721245
48-49	0.0024933715121901925
50-51	2.9424901405760784E-4
52-53	0.0015385301375395594
54-55	5.014868521698463E-4
56-57	0.008831410698308447
58-59	9.744958947067504E-5
60-61	0.002614599241096698
62-63	0.001329931973979531
64-65	0.0013330362903794242
66-67	0.004729262274495698
68-69	0.0020268321110224817
70-71	0.002582170998600966
72-73	0.003661861249594635
74-75	6.400328901607556E-4
76-77	3.553500165935767E-4
78-79	8.220073985803417E-4
80-81	4.680972237933819E-4
82-83	0.001487670600765492
84-85	2.3986669808032015E-4
86-87	4.249279173864586E-4
88-89	1.0929834492931848E-4
90-91	2.631098279967967E-4
92-93	0.0
94-95	0.0
96-97	5.794902325834754E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	938.0
20-21	2450.0
22-23	3284.0
24-25	4283.0
26-27	6351.0
28-29	9691.0
30-31	21057.0
32-33	26703.0
34-35	34983.0
36-37	50806.0
38-39	59969.0
40-41	64142.0
42-43	73827.0
44-45	89807.0
46-47	87456.0
48-49	80612.0
50-51	71998.0
52-53	62249.0
54-55	67689.0
56-57	78461.0
58-59	58061.0
60-61	91496.0
62-63	92941.0
64-65	100615.0
66-67	109115.0
68-69	112453.0
70-71	101369.0
72-73	88015.0
74-75	87747.0
76-77	92256.0
78-79	95403.0
80-81	95721.0
82-83	92041.0
84-85	90777.0
86-87	92602.0
88-89	97074.0
90-91	100649.0
92-93	100062.0
94-95	110539.0
96-97	162584.0
98-99	797759.0
100-101	5974705.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.35696418046348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52621652003184	72.90750442972217
2	7.397514934195981	12.18473744920044
3	2.113805187322357	5.222597342903556
4	0.8628498258253703	2.842467687944767
5	0.4353982582523658	1.7929039379563134
6	0.22204112663533676	1.097197986773771
7	0.1367182088460003	0.788178764012302
8	0.08563854397063822	0.5642344398605518
9	0.05828133837965207	0.4319886687590228
>10	0.1589045042990428	1.8645737394256072
>50	0.00194264140548105	0.10978036777440574
>100	5.807242660052773E-4	0.08789674743391547
>500	6.491232153984704E-5	0.03717229843731203
>1k	4.327424838358665E-5	0.06876613979580505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0372647742808125E-5	0.0	0.0	0.0
3	0.0	1.0372647742808125E-5	0.0	1.0372647742808125E-5	0.0
4	0.0	4.14905909712325E-5	0.0	1.0372647742808125E-5	0.0
5	0.0	5.186323871404062E-5	0.0	1.0372647742808125E-5	0.0
6	0.0	5.186323871404062E-5	0.0	1.0372647742808125E-5	0.0
7	0.0	5.186323871404062E-5	0.0	1.0372647742808125E-5	0.0
8	0.0	5.186323871404062E-5	0.0	1.0372647742808125E-5	0.0
9	0.0	7.260853419965687E-5	0.0	3.1117943228424376E-5	0.0
10-11	0.0	9.335382968527312E-5	0.0	3.1117943228424376E-5	0.0
12-13	0.0	1.1409912517088937E-4	0.0	3.1117943228424376E-5	0.0
14-15	0.0	1.244717729136975E-4	0.0	3.630426709982844E-5	0.0
16-17	0.0	1.244717729136975E-4	0.0	5.704956258544469E-5	0.0
18-19	0.0	1.2965809678510158E-4	0.0	9.335382968527313E-5	0.0
20-21	0.0	1.3484442065650563E-4	0.0	1.5558971614212187E-4	0.0
22-23	0.0	1.6596236388493E-4	0.0	2.3857089808458686E-4	0.0
24-25	0.0	1.8670765937054624E-4	0.0	3.3711105164126404E-4	0.0
26-27	0.0	2.1263927872756656E-4	0.0	5.186323871404062E-4	0.0
28-29	0.0	2.333845742131828E-4	0.0	0.001680368934334916	0.0
30-31	0.0	2.6968884131301125E-4	0.0	0.006762966328310897	0.0
32-33	0.0	2.9562046067003155E-4	0.0	0.019894738370705982	0.0
34-35	0.0	3.3711105164126404E-4	0.0	0.03716001053861011	0.0
36-37	0.0	3.5267002325547624E-4	0.0	0.06350653580534274	0.0
38-39	0.0	3.734153187410925E-4	0.0	0.10882982011754284	0.0
40-41	0.0	3.8378796648390063E-4	0.0	0.18918672218107738	0.0
42-43	0.0	4.097195858409209E-4	0.0	0.23763217346386273	0.0
44-45	0.0	4.615828245549615E-4	0.0	0.29761200903665075	0.0
46-47	0.0	4.8751444391198186E-4	0.0	0.3656099013146294	0.0
48-49	0.0	5.134460632690021E-4	0.0	0.4282658800050618	0.0
50-51	0.0	5.912409213400631E-4	0.0	0.4922599302543166	0.0
52-53	0.0	5.964272452114671E-4	0.0	0.5707808736673741	0.0
54-55	0.0	6.327315123112956E-4	0.0	0.7093801928067762	0.0
56-57	0.0	6.845947510253363E-4	0.0	0.8553440918435722	0.0
58-59	0.0	6.949673987681444E-4	0.0	0.9562129048185097	0.0
60-61	0.0	7.157126942537606E-4	0.0	1.3434549630007655	0.0
62-63	0.0	7.157126942537606E-4	0.0	1.3681989141912343	0.0
64-65	0.0	7.572032852249931E-4	0.0	1.3761806666293253	0.0
66-67	0.0	7.986938761962257E-4	0.0	1.3817041015523706	0.0
68-69	0.0	8.090665239390338E-4	0.0	1.3874557347257577	0.0
70-71	0.0	8.090665239390338E-4	0.0	1.3929739833249313	0.0
72-73	0.0	8.090665239390338E-4	0.0	1.39721639625174	0.0
74-75	0.0	8.194391716818419E-4	0.0	1.400452662347496	0.0
76-77	0.0	8.401844671674582E-4	0.0	1.4037926549206805	0.0
78-79	0.0	8.713024103958824E-4	0.0	1.4065310339247818	0.0
80-81	0.0	8.713024103958824E-4	0.0	1.4079002234268323	0.0
82-83	0.0	8.868613820100946E-4	0.0	1.4084759053765583	0.0
84-85	0.0	9.12793001367115E-4	0.0	1.4088285753998138	0.0
86-87	0.0	9.335382968527312E-4	0.0	1.4089374882011132	0.0
88-89	0.0	9.490972684669434E-4	0.0	1.4089789787920843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13805	0.0	41.83578	1
GTATCAA	23080	0.0	36.57438	1
ATCAACG	27205	0.0	30.429022	3
TCAACGC	27955	0.0	29.710121	4
TATCAAC	28600	0.0	29.272945	2
CAACGCA	28080	0.0	28.116043	5
AACGCAG	29275	0.0	27.188704	6
ACGCAGA	32690	0.0	24.237328	7
CGCAGAG	33035	0.0	23.901375	8
GCAGAGT	37645	0.0	20.702284	9
CTTATAC	23535	0.0	20.338541	1
TACATGG	22630	0.0	19.355215	2
GAGTACT	23155	0.0	19.15133	12-13
AGAGTAC	32475	0.0	19.147758	10-11
GTACATG	23735	0.0	18.94078	1
TATACAC	27285	0.0	18.848475	3
ACATGGG	22955	0.0	18.37237	3
CAGAGTA	36595	0.0	17.846867	10-11
ACATCTC	25735	0.0	17.65994	8
AGTACTT	25185	0.0	17.24436	12-13
>>END_MODULE