FastQCFastQC Report
Fri 27 May 2016
SRR2139971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15530453
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180650.042.7833061
GTATCAA299600.035.474891
ATCAACG340900.030.2251473
TCAACGC352200.029.3733484
CAACGCA352650.027.9278325
TATCAAC384100.027.3413332
AACGCAG371350.026.7688676
ACGCAGA415950.023.7682427
CGCAGAG422550.023.399468
TACATGG335650.019.8026872
GTACATG351150.019.7823051
GCAGAGT497500.019.5810999
ACATGGG334700.019.413113
AGAGTAC434350.018.6195110-11
GAGTACT308600.017.9989312-13
CGTCTTC275550.017.90581948-49
CATGGGG241500.017.6912944
CCGTCTT316050.017.21099746-47
TGCCGTC315000.017.20417444-45
TATGCCG314500.017.18446542-43
Produced by FastQC (version 0.11.5)