##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139971_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15530453
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.837071526503443	33.0	31.0	34.0	30.0	34.0
2	31.437530251049342	33.0	31.0	34.0	28.0	34.0
3	31.779504821913438	33.0	31.0	34.0	30.0	34.0
4	32.88990681727056	37.0	35.0	37.0	26.0	37.0
5	34.408106254209066	37.0	35.0	37.0	28.0	37.0
6	35.16733909822205	37.0	35.0	37.0	32.0	37.0
7	35.29331333735082	37.0	35.0	37.0	32.0	37.0
8	35.434824148400565	37.0	35.0	37.0	32.0	37.0
9	37.20111197014021	39.0	37.0	39.0	33.0	39.0
10-11	37.05949282355125	39.0	37.0	39.0	33.0	39.0
12-13	36.9563673706105	39.0	37.0	39.0	32.5	39.0
14-15	38.31937226814955	40.0	38.0	41.0	33.0	41.0
16-17	38.31487265696629	40.0	38.0	41.0	33.0	41.0
18-19	38.236441493367906	40.0	38.0	41.0	33.0	41.0
20-21	38.31037712468283	40.0	38.0	41.0	32.5	41.0
22-23	38.354979170729905	40.0	38.0	41.0	33.0	41.0
24-25	38.23020019351795	40.0	38.0	41.0	32.0	41.0
26-27	38.13949703116816	40.0	38.0	41.0	32.0	41.0
28-29	38.1588857932202	40.0	38.0	41.0	32.0	41.0
30-31	38.11278104279744	40.0	38.0	41.0	32.0	41.0
32-33	38.02055844974633	40.0	37.0	41.0	32.0	41.0
34-35	37.9007952367147	40.0	37.0	41.0	31.0	41.0
36-37	37.85173907468304	40.0	37.0	41.0	31.0	41.0
38-39	37.77341689120571	40.0	37.0	41.0	31.0	41.0
40-41	37.708651831697	40.0	37.0	41.0	31.0	41.0
42-43	37.688196572828176	40.0	37.0	41.0	31.0	41.0
44-45	37.6279672552898	40.0	37.0	41.0	31.0	41.0
46-47	37.49808698975063	40.0	36.0	41.0	30.0	41.0
48-49	37.669569160993554	40.0	37.0	41.0	31.0	41.0
50-51	37.59961771243674	40.0	36.5	41.0	30.0	41.0
52-53	37.54774463640296	40.0	36.0	41.0	30.5	41.0
54-55	37.432154029421696	39.5	36.0	41.0	30.0	41.0
56-57	37.295772487956285	39.0	36.0	41.0	30.0	41.0
58-59	37.202577245062145	39.0	35.0	41.0	30.5	41.0
60-61	37.00263726300423	39.0	35.0	41.0	30.5	41.0
62-63	36.84299863114313	39.0	35.0	41.0	30.0	41.0
64-65	36.61719986403398	38.5	35.0	40.5	30.0	41.0
66-67	36.42027550974949	38.0	35.0	40.0	30.0	41.0
68-69	36.17865538795381	37.0	34.5	40.0	30.0	41.0
70-71	35.93721547885285	37.0	34.0	39.5	30.0	41.0
72-73	35.63606930840735	36.5	34.0	39.0	30.0	41.0
74-75	35.45781168546352	36.0	34.0	39.0	30.0	41.0
76-77	35.23963968276263	36.0	34.0	39.0	30.0	40.0
78-79	34.95444191668366	35.0	34.0	37.0	30.0	39.0
80-81	34.66870456428431	35.0	34.0	37.0	30.0	39.0
82-83	34.38692628510712	35.0	34.0	36.5	30.0	39.0
84-85	34.156293252467584	35.0	34.0	36.0	30.0	37.0
86-87	33.96675087278698	35.0	34.0	36.0	29.5	37.0
88-89	33.80945190528139	35.0	34.0	35.5	29.0	37.0
90-91	33.67001775252385	35.0	34.0	35.0	29.0	36.0
92-93	33.62573058719308	35.0	34.0	35.0	29.0	36.0
94-95	33.588809324715	35.0	34.0	35.0	29.0	36.0
96-97	33.576365708832654	35.0	34.0	35.0	29.5	36.0
98-99	33.59894952490444	35.0	34.0	35.0	30.0	35.5
100-101	33.205830958937504	34.5	33.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	111.0
18	707.0
19	2498.0
20	7081.0
21	15779.0
22	28092.0
23	46369.0
24	73739.0
25	114561.0
26	176109.0
27	255560.0
28	293988.0
29	235276.0
30	274097.0
31	339504.0
32	424675.0
33	557523.0
34	764176.0
35	1074451.0
36	1628126.0
37	2819737.0
38	4002578.0
39	2274258.0
40	121454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88364666503932	21.06235407299452	13.905441135554772	25.14855812641138
2	13.811713700222588	21.038803946925793	42.52130169958156	22.628180653270057
3	17.07238104142865	26.85938630718332	31.341144067529214	24.72708858385882
4	11.378504879342696	18.931227616538905	39.58003475676801	30.11023274735039
5	12.309016460365854	38.23695158835615	36.67609634680275	12.77793560447525
6	28.439203770985728	39.41322794994246	19.148967429683708	12.998600849388103
7	25.109441559344088	33.01965958070047	23.964276667437616	17.90662219251783
8	23.148582184272776	37.80528574725338	21.909738391260273	17.136393677213572
9	24.669553175379583	16.70078789286388	21.699979478971933	36.929679452784605
10-11	23.20457096857821	27.13580842212379	30.00059805761736	19.65902255168064
12-13	24.246439223633253	24.48338722077608	29.688995580011003	21.581177975579667
14-15	21.307115279740724	26.072117644290067	26.986408984392497	25.634358091576715
16-17	21.198125911456604	29.33094670691496	27.67851655358276	21.79241082804568
18-19	21.106983015079987	28.408850611716442	29.253021208329304	21.231145164874267
20-21	21.765381540830823	27.612138349263375	29.04229380764952	21.580186302256273
22-23	21.42593938805226	27.990656097420125	28.884062348896638	21.699342165630977
24-25	21.471154205547446	27.96380854049397	29.05937232442637	21.505664929532216
26-27	21.585399947882415	28.08326983298901	28.973914830209583	21.357415388918984
28-29	21.362178080599712	27.948468058267405	29.129529083592264	21.559824777540616
30-31	21.363301720823536	27.58971796719597	29.419069210627136	21.62791110135336
32-33	21.069325072073685	28.11592701551953	29.07461498371424	21.74013292869254
34-35	21.475880244374267	27.91595174257942	29.126134890017052	21.48203312302926
36-37	21.275554874828835	27.886859517708018	29.440453520752353	21.397132086710798
38-39	21.202432047707877	27.768297475216634	29.406135438506197	21.623135038569288
40-41	21.392497065536535	27.607893271680677	29.453186792489028	21.54642287029376
42-43	21.77826276081585	27.527531890083694	29.263530153459982	21.430675195640475
44-45	21.25255760939777	28.086085259361955	29.002843464054116	21.65851366718616
46-47	21.66756116043233	27.567058737098606	28.57744925529751	22.187930847171554
48-49	21.58063757885225	27.67427044667061	28.688605415584767	22.05648655889237
50-51	21.122372500802435	27.86832995068142	28.969032810589983	22.040264737926158
52-53	21.264030844275574	27.8546063265493	28.98058768365766	21.900775145517475
54-55	21.265034457346186	27.634633248967532	28.732575729489163	22.367756564197116
56-57	21.706351415242732	27.617793432554972	28.51861043949122	22.157244712711073
58-59	21.082143433885626	27.75401111744438	29.280530189272152	21.883315259397833
60-61	21.46218366385816	28.060718686493036	28.64922052506566	21.827877124583146
62-63	20.862338060707515	28.825234852365593	28.651343047722293	21.661084039204596
64-65	20.455138214589773	28.935846653327374	28.55069932549047	22.058315806592375
66-67	20.65172655547051	29.36281118125534	28.071357980358663	21.914104282915492
68-69	20.888565483759017	29.31543125164322	27.784886067584146	22.011117197013615
70-71	20.97021189917583	28.617000907546842	27.85540703599671	22.557380157280623
72-73	20.864102538973693	28.208177564289134	27.982991915849652	22.94472798088752
74-75	21.36575783889983	27.863346209650775	27.948561541678156	22.822334409771237
76-77	21.57646080129357	27.809356340533025	27.847105188674053	22.767077669499347
78-79	21.913325905203845	27.580778151564555	27.215870699825413	23.290025243406184
80-81	21.7148112316612	28.043110458031162	27.19522894695885	23.046849363348787
82-83	21.76402028830472	28.10759009254749	27.33382833107887	22.79456128806892
84-85	21.884002155972595	28.100700985893972	27.183249809321552	22.832047048811884
86-87	22.00328846480453	28.534339655820624	27.054447546000247	22.4079243333746
88-89	22.24192585472748	28.232979367012984	27.394000805675624	22.131093972583912
90-91	21.987792965357766	28.224148512173088	27.71668918570172	22.071369336767425
92-93	21.822839093648625	27.779089024999138	27.558329576170397	22.83974230518184
94-95	21.54068105593054	27.8254169558879	28.256603296584405	22.377298691597154
96-97	21.198577553875875	28.079114704160475	28.488024661827975	22.234283080135672
98-99	21.488732017870394	28.201506109383562	28.7387889102614	21.57097296248465
100-101	22.246098139579964	30.995456248394948	26.080862518541064	20.677583093484028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2474.0
1	2247.0
2	3487.5
3	5133.0
4	6957.0
5	11784.5
6	15637.0
7	17614.5
8	17963.0
9	16155.0
10	13528.5
11	11317.5
12	12467.5
13	17709.0
14	30454.5
15	48241.5
16	62525.5
17	70303.0
18	72295.5
19	69979.5
20	65788.5
21	62453.0
22	63247.0
23	72841.5
24	90864.5
25	115728.0
26	148535.5
27	184023.5
28	218049.0
29	252110.0
30	284178.5
31	317047.5
32	355180.0
33	394682.0
34	434687.0
35	473484.0
36	512189.5
37	547779.0
38	571325.5
39	587105.5
40	607310.0
41	625264.5
42	634931.5
43	647104.5
44	662395.5
45	686713.0
46	723520.0
47	739649.0
48	722217.0
49	681622.0
50	635629.0
51	597162.5
52	553931.0
53	510635.0
54	469219.0
55	424986.0
56	380760.0
57	332546.5
58	284297.0
59	241074.0
60	197934.0
61	156057.0
62	119393.0
63	90559.0
64	66995.0
65	48622.0
66	35759.5
67	26960.0
68	20897.0
69	16044.5
70	12241.0
71	9291.0
72	6782.0
73	4854.0
74	3459.5
75	2426.0
76	1740.0
77	1181.5
78	854.5
79	646.5
80	452.5
81	316.5
82	241.0
83	164.0
84	104.5
85	75.5
86	57.5
87	48.5
88	39.0
89	26.0
90	18.5
91	16.5
92	13.5
93	8.5
94	5.0
95	5.0
96	5.0
97	4.5
98	3.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.8831839612147825
3	0.006793105133507696
4	8.908742069532678
5	3.6702084607577126E-4
6	0.07567068391372743
7	0.026592913934963776
8	5.47311788007729E-4
9	2.7043641289793675E-4
10-11	0.30259902914615566
12-13	0.3182070735476937
14-15	6.117014101262855E-5
16-17	1.5131561197860745E-4
18-19	0.5053394128297481
20-21	2.575950781823602E-4
22-23	1.674785673329383E-4
24-25	0.18253559389309926
26-27	0.16865971418357414
28-29	1.1934977981901025E-4
30-31	1.130697593248792E-4
32-33	1.1661477794942826E-4
34-35	3.217818419221674E-4
36-37	3.3963648576299367E-4
38-39	0.0014431182768234185
40-41	0.00175666823170837
42-43	0.0011643893152309607
44-45	9.093043103977035E-4
46-47	0.0014367719691752772
48-49	0.0025010383072507428
50-51	3.2798157171669214E-4
52-53	0.001636973098415066
54-55	6.40528486129373E-4
56-57	0.011114149358334857
58-59	1.9915180199363533E-4
60-61	0.0026981892834167147
62-63	0.0014861720206161499
64-65	0.001590350314325739
66-67	0.004879637962156934
68-69	0.0020496567951256133
70-71	0.0026735955664360937
72-73	0.0035895010171962173
74-75	7.262617597245585E-4
76-77	3.5773399312443046E-4
78-79	8.072847167574625E-4
80-81	4.388601397817421E-4
82-83	0.001637209163940747
84-85	2.9479939474408713E-4
86-87	5.889257815408015E-4
88-89	1.3869201160910977E-4
90-91	2.77038558397043E-4
92-93	0.0
94-95	0.0
96-97	2.6785365119347552E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1362.0
20-21	3602.0
22-23	4905.0
24-25	6306.0
26-27	9942.0
28-29	17734.0
30-31	39758.0
32-33	49043.0
34-35	65143.0
36-37	93825.0
38-39	69978.0
40-41	111036.0
42-43	121122.0
44-45	144019.0
46-47	144023.0
48-49	135405.0
50-51	121188.0
52-53	23046.0
54-55	24643.0
56-57	26689.0
58-59	30390.0
60-61	151065.0
62-63	164422.0
64-65	181536.0
66-67	200480.0
68-69	205937.0
70-71	182398.0
72-73	152390.0
74-75	151322.0
76-77	159199.0
78-79	164528.0
80-81	165865.0
82-83	157971.0
84-85	155294.0
86-87	157407.0
88-89	164327.0
90-91	169064.0
92-93	169043.0
94-95	181552.0
96-97	267692.0
98-99	1352933.0
100-101	9632869.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.79729396364719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.56203694142314	69.87213601870478
2	7.677793870582569	12.253343489663317
3	2.2844306821526557	5.468741600499316
4	1.0397906981952683	3.318899360182151
5	0.5021170775867014	2.0033792022176726
6	0.30892247907078163	1.4790706724633886
7	0.18003924089441561	1.0056650961450502
8	0.11715211290008931	0.7478737273240649
9	0.07794011511923188	0.5597469249956865
>10	0.24602253783000327	2.9190590168538484
>50	0.002725540678395219	0.14489000579494413
>100	9.340346694247062E-4	0.12298410459673326
>500	4.73355002399374E-5	0.032194512952835944
>1k	4.7333397135545304E-5	0.07201626760618549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.897532995335036E-5	0.0
12-13	0.0	0.0	0.0	5.151169769484509E-5	0.0
14-15	0.0	0.0	0.0	1.1912080091932927E-4	0.0
16-17	0.0	0.0	0.0	2.1248575299123598E-4	0.0
18-19	0.0	0.0	0.0	2.865338184275758E-4	0.0
20-21	0.0	0.0	0.0	3.8311825160541033E-4	0.0
22-23	0.0	0.0	0.0	5.183364580543787E-4	0.0
24-25	0.0	0.0	0.0	7.43700135469326E-4	0.0
26-27	0.0	0.0	0.0	0.0010881846138036024	0.0
28-29	0.0	0.0	0.0	0.00374747600729998	0.0
30-31	0.0	0.0	0.0	0.013222408902045547	0.0
32-33	0.0	0.0	0.0	0.03431644910808461	0.0
34-35	0.0	0.0	0.0	0.061955694402474934	0.0
36-37	0.0	0.0	0.0	0.10404397090026929	0.0
38-39	0.0	0.0	0.0	0.18350076459456782	0.0
40-41	0.0	0.0	0.0	0.5433099729930608	0.0
42-43	0.0	0.0	0.0	0.6216785820735558	0.0
44-45	0.0	0.0	0.0	0.7249209021784491	0.0
46-47	0.0	0.0	0.0	0.841424264958659	0.0
48-49	0.0	0.0	0.0	0.9496406833722106	0.0
50-51	0.0	0.0	0.0	1.0569202327839373	0.0
52-53	0.0	0.0	0.0	1.3070385004223637	0.0
54-55	0.0	0.0	0.0	2.0270110601410014	0.0
56-57	0.0	0.0	0.0	2.8146571127062425	0.0
58-59	0.0	0.0	0.0	3.6276469205373467	0.0
60-61	0.0	0.0	0.0	4.250500613214566	0.0
62-63	0.0	0.0	0.0	4.283474538701479	0.0
64-65	0.0	0.0	0.0	4.292089870140941	0.0
66-67	0.0	0.0	0.0	4.298319566080912	0.0
68-69	0.0	0.0	0.0	4.304410824333328	0.0
70-71	0.0	0.0	0.0	4.309880722732299	0.0
72-73	0.0	0.0	0.0	4.314130437792123	0.0
74-75	0.0	0.0	0.0	4.317498015028924	0.0
76-77	0.0	0.0	0.0	4.320585497409509	0.0
78-79	0.0	0.0	0.0	4.323408982339408	0.0
80-81	0.0	0.0	0.0	4.324896382610346	0.0
82-83	0.0	0.0	0.0	4.325456572322778	0.0
84-85	0.0	0.0	0.0	4.325920177602031	0.0
86-87	0.0	0.0	0.0	4.326029639959632	0.0
88-89	0.0	0.0	0.0	4.326061834770692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18065	0.0	42.783306	1
GTATCAA	29960	0.0	35.47489	1
ATCAACG	34090	0.0	30.225147	3
TCAACGC	35220	0.0	29.373348	4
CAACGCA	35265	0.0	27.927832	5
TATCAAC	38410	0.0	27.341333	2
AACGCAG	37135	0.0	26.768867	6
ACGCAGA	41595	0.0	23.768242	7
CGCAGAG	42255	0.0	23.39946	8
TACATGG	33565	0.0	19.802687	2
GTACATG	35115	0.0	19.782305	1
GCAGAGT	49750	0.0	19.581099	9
ACATGGG	33470	0.0	19.41311	3
AGAGTAC	43435	0.0	18.61951	10-11
GAGTACT	30860	0.0	17.99893	12-13
CGTCTTC	27555	0.0	17.905819	48-49
CATGGGG	24150	0.0	17.691294	4
CCGTCTT	31605	0.0	17.210997	46-47
TGCCGTC	31500	0.0	17.204174	44-45
TATGCCG	31450	0.0	17.184465	42-43
>>END_MODULE