FastQCFastQC Report
Fri 27 May 2016
SRR2139970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10968284
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111650.051.4400751
GTATCAA197850.039.6033021
CTTATAC163600.032.4354821
ATCAACG240200.031.9687883
TCAACGC247250.031.1106744
TATCAAC262100.029.719692
CAACGCA253750.028.8151935
AACGCAG265300.027.7262426
TATACAC213350.026.9510843
TTATACA219850.025.0987822
ACGCAGA301550.024.3034217
CGCAGAG311900.023.5452868
ACATCTC245100.020.425738
GCAGAGT364200.020.0591039
GTGGTAT62650.019.4501381
TACATGG251850.019.262312
TACACAT277450.019.035835
ACACATC265700.018.893996
GTACATG264200.018.8840351
ATACACA322000.018.7949834
Produced by FastQC (version 0.11.5)