##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10968284
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.769298643251762	33.0	31.0	34.0	30.0	34.0
2	31.472830481048813	33.0	31.0	34.0	29.0	34.0
3	31.798098316929067	33.0	31.0	34.0	30.0	34.0
4	33.17264970527751	37.0	35.0	37.0	28.0	37.0
5	34.52938253604666	37.0	35.0	37.0	30.0	37.0
6	35.121625588834135	37.0	35.0	37.0	32.0	37.0
7	35.24729055155756	37.0	35.0	37.0	32.0	37.0
8	35.385309497821176	37.0	35.0	37.0	32.0	37.0
9	37.131854809740524	39.0	37.0	39.0	33.0	39.0
10-11	37.01961108957427	39.0	37.0	39.0	33.0	39.0
12-13	36.91818013647349	39.0	37.0	39.0	32.5	39.0
14-15	38.24735947756276	40.0	38.0	41.0	33.0	41.0
16-17	38.23681120036644	40.0	38.0	41.0	32.5	41.0
18-19	38.180486984108	40.0	38.0	41.0	32.5	41.0
20-21	38.237116749517966	40.0	38.0	41.0	32.5	41.0
22-23	38.27027743397579	40.0	38.0	41.0	32.5	41.0
24-25	38.16041242255025	40.0	38.0	41.0	32.0	41.0
26-27	38.071216577824444	40.0	38.0	41.0	32.0	41.0
28-29	38.060953365628194	40.0	38.0	41.0	32.0	41.0
30-31	38.01353185476664	40.0	37.0	41.0	32.0	41.0
32-33	37.91410042453035	40.0	37.0	41.0	32.0	41.0
34-35	37.7975076511132	40.0	37.0	41.0	31.0	41.0
36-37	37.75083405252029	40.0	37.0	41.0	31.0	41.0
38-39	37.66766945952311	40.0	37.0	41.0	31.0	41.0
40-41	37.59176865579158	40.0	37.0	41.0	31.0	41.0
42-43	37.571341608524435	40.0	37.0	41.0	31.0	41.0
44-45	37.50713341515044	40.0	36.5	41.0	31.0	41.0
46-47	37.377480271627846	40.0	36.0	41.0	30.0	41.0
48-49	37.55702488213079	40.0	36.5	41.0	31.0	41.0
50-51	37.491177385128395	40.0	36.0	41.0	30.5	41.0
52-53	37.43424777818963	39.5	36.0	41.0	30.0	41.0
54-55	37.30810324756484	39.0	36.0	41.0	30.0	41.0
56-57	37.152553497744194	39.0	35.0	41.0	30.0	41.0
58-59	37.03551366471609	39.0	35.0	41.0	30.5	41.0
60-61	36.821584605333356	39.0	35.0	41.0	30.0	41.0
62-63	36.64476142565461	38.5	35.0	40.5	30.0	41.0
64-65	36.39157205546766	38.0	35.0	40.0	30.0	41.0
66-67	36.17024781166448	37.5	34.5	40.0	30.0	41.0
68-69	35.91106079677106	37.0	34.0	40.0	30.0	41.0
70-71	35.65800714423652	36.5	34.0	39.0	30.0	41.0
72-73	35.35657330146671	36.0	34.0	39.0	30.0	41.0
74-75	35.17981650830055	36.0	34.0	39.0	30.0	40.0
76-77	34.98854570730641	35.0	34.0	37.5	30.0	39.5
78-79	34.72951882377011	35.0	34.0	37.0	30.0	39.0
80-81	34.47822647130869	35.0	34.0	37.0	30.0	39.0
82-83	34.234354131666365	35.0	34.0	36.0	30.0	38.5
84-85	34.027944251386224	35.0	34.0	36.0	29.0	37.0
86-87	33.848109177361195	35.0	34.0	35.5	29.0	37.0
88-89	33.71804058497075	35.0	34.0	35.0	29.0	36.5
90-91	33.591459259023424	35.0	34.0	35.0	29.0	36.0
92-93	33.554034271808504	35.0	34.0	35.0	29.0	36.0
94-95	33.53514379965556	35.0	34.0	35.0	29.0	36.0
96-97	33.5497570222028	35.0	34.0	35.0	30.0	36.0
98-99	33.590742816503194	35.0	34.0	35.0	30.0	35.0
100-101	33.200944205351284	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	6.0
17	101.0
18	673.0
19	2668.0
20	7516.0
21	15637.0
22	26560.0
23	40697.0
24	60932.0
25	89330.0
26	126876.0
27	171971.0
28	188411.0
29	171754.0
30	204183.0
31	251859.0
32	315075.0
33	409416.0
34	558244.0
35	786547.0
36	1208918.0
37	2080646.0
38	2722165.0
39	1454438.0
40	73659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.73740103739108	20.592902226091155	14.523976585580753	25.145720150937013
2	14.204367744020303	20.66241202402552	41.870893960416	23.26232627153818
3	17.401599449727	26.08137369847881	31.052503786819543	25.46452306497465
4	12.00550147840096	18.533746832528667	38.617372632928785	30.84337905614159
5	13.137523520134732	37.480824218093986	35.76028727249586	13.62136498927542
6	29.43186682885331	38.377432845277085	18.70654509237595	13.484155233493658
7	25.75747171498558	32.88823996157504	23.09911306883618	18.255175254603202
8	23.947927427859604	37.000779797580854	21.31928111287955	17.73201166167999
9	24.998543525077764	16.92146696336512	21.130456116889246	36.949533394667874
10-11	23.808828663122604	26.74379117934434	29.06809805189291	20.379282105640147
12-13	24.79168396986476	24.10061956914797	28.938724029375724	22.168972431611554
14-15	21.954179490012834	25.6407296825864	26.44188111240089	25.96320971499988
16-17	21.85601142495268	28.822604980747652	27.033184713764875	22.288198880534793
18-19	21.705961380621986	27.798691059073345	28.68876319967162	21.806584360633053
20-21	22.48234470795356	27.09179037525728	28.510282314606723	21.915582602182436
22-23	22.223121692612537	27.264149020159778	28.331762097916023	22.180967189311666
24-25	22.159312734549268	27.437160170875057	28.448349445781968	21.955177648793704
26-27	22.10857608629724	27.71701091353634	28.312616328468426	21.861796671698
28-29	21.847697589077132	27.484020100631046	28.46150915877001	22.206773151521812
30-31	22.5767224404835	27.328607730862466	28.340169290260974	21.754500538393064
32-33	22.367642040865494	27.461183573493003	28.439764933284025	21.73140945235748
34-35	22.24256019171868	27.58358579820559	28.48851944736156	21.685334562714168
36-37	22.287739545404712	27.27237055459702	28.336412055603965	22.103477844394305
38-39	21.90098827474887	27.204728807888067	28.70990290915909	22.18438000820397
40-41	22.163113607607773	27.048058121492808	28.689136445056796	22.09969182584263
42-43	22.306548879440314	27.052411363135874	28.587343802054217	22.053695955369594
44-45	22.088109993126974	27.469313956944998	28.267624382951755	22.174951666976273
46-47	22.428634012903544	27.05902931755646	27.78498293057514	22.727353738964855
48-49	22.299747635491286	27.12250584386674	28.050550668430795	22.527195852211182
50-51	22.045343105565575	27.45969612859378	28.02437723730824	22.470583528532405
52-53	22.121400799568466	27.58184126410141	28.064916133648076	22.231841802682055
54-55	22.153876129254876	27.464038853011196	27.84398211635081	22.538102901383116
56-57	22.43335091683056	27.652648142214726	27.523820886443374	22.390180054511344
58-59	22.051491714069385	28.029938260346132	27.955869672834098	21.962700352750385
60-61	22.333154895701355	28.164677922458676	27.392538759090858	22.109628422749118
62-63	21.999944629031557	28.468332609688495	27.336843398194844	22.194879363085104
64-65	21.977511317681994	28.43603905995018	27.32718203613384	22.25926758623399
66-67	22.1212706285117	28.31313020175978	27.203631273982182	22.36196789574634
68-69	22.238380064877397	28.063556365353936	27.41352249518218	22.284541074586485
70-71	22.29287220642559	27.538865916908183	27.5020107277954	22.666251148870828
72-73	22.296315122389164	27.485680555922297	27.626041914217563	22.591962407470977
74-75	22.190852591091424	27.639717475328535	27.512419087049928	22.65701084653011
76-77	22.31995023160627	27.609979303101877	27.417172334311907	22.652898130979942
78-79	22.412111820472404	27.587683293403302	27.394134201322018	22.606070684802276
80-81	22.353479707877685	27.56937766738584	27.40200392240089	22.67513870233558
82-83	22.427689347190626	27.600986587454134	27.485122281253705	22.48620178410153
84-85	22.6493173006325	27.573466111109507	27.341855415404424	22.435361172853565
86-87	22.345400025503196	27.65336903302616	27.423188953459537	22.57804198801111
88-89	22.468745742993516	27.543445446077996	27.41377525087555	22.57403356005294
90-91	22.538026518370017	27.562319641384747	27.402164410705105	22.49748942954013
92-93	22.5394240917754	27.60974562640804	27.376127136613622	22.474703145202948
94-95	22.371438494025554	27.722342664765243	27.461410808537995	22.444808032671208
96-97	22.286303643543658	27.684315992470875	27.622090825903555	22.407289538081912
98-99	22.6031329675029	28.22052438060877	27.623073928314728	21.553268723573606
100-101	23.291126299335463	30.81216112779874	24.64373278257864	21.25297979028716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1986.0
1	1762.0
2	2336.0
3	3259.0
4	4238.5
5	6789.0
6	8930.5
7	10345.5
8	10925.5
9	10150.0
10	8722.0
11	7477.5
12	8445.5
13	12148.5
14	20981.0
15	33272.5
16	43298.5
17	49412.5
18	51385.5
19	49547.0
20	45932.5
21	42894.0
22	42516.5
23	47627.5
24	57962.0
25	73321.0
26	93842.0
27	116866.0
28	139820.5
29	162216.5
30	182057.0
31	201652.5
32	224935.0
33	248547.5
34	274713.5
35	302689.5
36	333007.0
37	359190.5
38	373495.0
39	384705.5
40	403459.5
41	421697.5
42	432714.0
43	442321.5
44	454502.5
45	471738.0
46	491809.0
47	503202.5
48	501946.5
49	491130.5
50	471315.5
51	451434.0
52	428299.5
53	403355.0
54	379135.0
55	349029.5
56	317546.0
57	287046.0
58	256551.0
59	224085.5
60	186665.5
61	149960.5
62	117787.0
63	89774.5
64	66966.0
65	48954.5
66	36190.5
67	27556.5
68	20096.5
69	14676.5
70	10890.5
71	7972.5
72	5764.5
73	4047.0
74	2864.5
75	2070.0
76	1472.0
77	973.5
78	656.5
79	463.0
80	335.5
81	254.5
82	189.5
83	126.5
84	88.5
85	70.0
86	57.0
87	42.0
88	27.0
89	19.5
90	12.5
91	8.5
92	7.0
93	3.0
94	1.0
95	1.0
96	1.0
97	1.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.5902943432172252
3	0.005807654141705302
4	7.970891344534843
5	2.0057832200552064E-4
6	0.07359400978311649
7	0.022747405154717
8	4.832114121042088E-4
9	1.9146112555072425E-4
10-11	0.25970334101487524
12-13	0.2716514269688859
14-15	7.293757163837114E-5
16-17	1.0028916100276032E-4
18-19	0.40423825641276245
20-21	1.459009293478853E-4
22-23	1.368206609049054E-4
24-25	0.1542589518302459
26-27	0.1357359281868981
28-29	7.764876258246812E-5
30-31	8.68928374417029E-5
32-33	1.5585036128405662E-4
34-35	2.988239759440263E-4
36-37	2.631313872259037E-4
38-39	0.001490511224454971
40-41	0.0017336481812885716
42-43	0.0011763526761788112
44-45	8.825488351992361E-4
46-47	0.0013462568239291067
48-49	0.002529287871987276
50-51	2.2443167070448518E-4
52-53	0.0014902968395870057
54-55	4.809008730928872E-4
56-57	0.007418034718003135
58-59	1.7178224074775798E-4
60-61	0.002677259212083636
62-63	0.0014100803389399264
64-65	0.0013685923108722959
66-67	0.005056698496362139
68-69	0.0021123174969745343
70-71	0.0028211362134253144
72-73	0.003861644856074516
74-75	7.174351991950488E-4
76-77	4.105729393512903E-4
78-79	8.479937123258162E-4
80-81	4.262960118855937E-4
82-83	0.0016271009941587074
84-85	2.301994530697097E-4
86-87	5.318116906192063E-4
88-89	1.3313765325883756E-4
90-91	2.3917438474229327E-4
92-93	0.0
94-95	0.0
96-97	5.77406099333096E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1217.0
20-21	2942.0
22-23	3821.0
24-25	4901.0
26-27	6439.0
28-29	10372.0
30-31	23842.0
32-33	29666.0
34-35	40314.0
36-37	58858.0
38-39	68612.0
40-41	71650.0
42-43	83658.0
44-45	100004.0
46-47	99357.0
48-49	93083.0
50-51	84520.0
52-53	78818.0
54-55	86687.0
56-57	97037.0
58-59	108587.0
60-61	107628.0
62-63	106098.0
64-65	116259.0
66-67	125134.0
68-69	129665.0
70-71	114855.0
72-73	99440.0
74-75	99187.0
76-77	103396.0
78-79	106235.0
80-81	106811.0
82-83	102880.0
84-85	102644.0
86-87	104857.0
88-89	108184.0
90-91	112072.0
92-93	113434.0
94-95	124117.0
96-97	188805.0
98-99	884430.0
100-101	6757768.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.99193693004793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.35508374947626	75.05106558665162
2	7.195303262804628	12.086949156841088
3	1.8982353037596749	4.78309379735319
4	0.712234794742332	2.392879198375343
5	0.3329203616016109	1.398131300718563
6	0.18079871858311813	0.9111380740960043
7	0.10067381345859501	0.5919052013365204
8	0.06274005944563076	0.4215727292755901
9	0.03929790574053064	0.2970636498397456
>10	0.11681927902136345	1.4632529990560188
>50	0.003763240046778892	0.21854883007883874
>100	0.002013787117424175	0.29846082952952513
>500	9.643772241712032E-5	0.057638840357103774
>1k	1.9286479692895853E-5	0.02829980649086722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.8234392909592786E-5	0.0	2.735158936438918E-5	0.0
5	0.0	1.8234392909592786E-5	0.0	5.470317872877836E-5	0.0
6	0.0	1.8234392909592786E-5	0.0	5.470317872877836E-5	0.0
7	0.0	1.8234392909592786E-5	0.0	6.382037518357476E-5	1.8234392909592786E-5
8	0.0	2.735158936438918E-5	0.0	7.293757163837114E-5	4.5585982273981966E-5
9	0.0	6.382037518357476E-5	0.0	2.55281500734299E-4	4.5585982273981966E-5
10-11	0.0	6.382037518357476E-5	0.0	2.643986971890954E-4	4.5585982273981966E-5
12-13	0.0	6.382037518357476E-5	0.0	2.735158936438918E-4	5.014458050138016E-5
14-15	0.0	8.205476809316754E-5	0.0	2.8719168832608635E-4	6.382037518357476E-5
16-17	0.0	9.117196454796393E-5	0.0	3.0086748300828096E-4	6.382037518357476E-5
18-19	0.0	9.573056277536213E-5	0.0	3.1454327769047557E-4	8.661336632056574E-5
20-21	0.0	1.0028916100276032E-4	0.0	3.373362688274665E-4	1.1852355391235311E-4
22-23	0.0	1.0028916100276032E-4	0.0	3.692464564192539E-4	1.1852355391235311E-4
24-25	0.0	1.1396495568495491E-4	0.0	4.1939103692063405E-4	1.1852355391235311E-4
26-27	0.0	1.1852355391235311E-4	0.0	5.151215996959962E-4	1.1852355391235311E-4
28-29	0.0	1.1852355391235311E-4	0.0	0.0014861030221318121	1.367579468219459E-4
30-31	0.0	1.367579468219459E-4	0.0	0.00538370450655727	1.4587514327674229E-4
32-33	0.0	1.4587514327674229E-4	0.0	0.01430943983580294	1.5043374150414048E-4
34-35	0.0	1.641095361863351E-4	0.0	0.026622213648005467	1.6410953618633506E-4
36-37	0.0	1.7322673264113148E-4	0.0	0.044104437850077546	1.6410953618633506E-4
38-39	0.0	1.8234392909592786E-4	0.0	0.0722993678865354	1.6866813441373326E-4
40-41	0.0	2.0057832200552064E-4	0.0	0.12083020461541659	1.8234392909592786E-4
42-43	0.0	2.0057832200552064E-4	0.0	0.14515032615858597	1.9146112555072425E-4
44-45	0.0	2.1425411668771525E-4	0.0	0.1771881545007405	2.0969551846031703E-4
46-47	0.0	2.2792991136990983E-4	0.0	0.21056621072175008	2.0969551846031703E-4
48-49	0.0	2.3704710782470622E-4	0.0	0.2372841549325309	2.0969551846031703E-4
50-51	0.0	2.507229025069008E-4	0.0	0.26144472553774134	2.3704710782470622E-4
52-53	0.0	2.55281500734299E-4	0.0	0.29632256057556494	2.55281500734299E-4
54-55	0.0	2.5984009896169724E-4	0.0	0.36962482007212794	2.55281500734299E-4
56-57	0.0	2.643986971890954E-4	0.0	0.44586281682713536	2.826330900986882E-4
58-59	0.0	2.643986971890954E-4	0.0	0.4910658768500159	3.0998467946307735E-4
60-61	4.5585982273981964E-6	2.643986971890954E-4	0.0	0.5115020727034421	3.1910187591787373E-4
62-63	9.117196454796393E-6	2.643986971890954E-4	0.0	0.5240564522216966	3.418948670548648E-4
64-65	9.117196454796393E-6	2.9175028655348457E-4	0.0	0.5336431842939151	3.4645346528226295E-4
66-67	9.117196454796393E-6	2.9175028655348457E-4	0.0	0.5394098110515738	3.510120635096611E-4
68-69	9.117196454796393E-6	2.9175028655348457E-4	0.0	0.5454727466940135	3.829222511014485E-4
70-71	9.117196454796393E-6	2.9175028655348457E-4	0.0	0.5512940766304009	4.148324386932359E-4
72-73	9.117196454796393E-6	2.9175028655348457E-4	0.0	0.5557979716790703	4.239496351480322E-4
74-75	9.117196454796393E-6	3.0086748300828096E-4	0.0	0.5593172095106217	4.3762542983022683E-4
76-77	9.117196454796393E-6	3.0998467946307735E-4	0.0	0.5627954199581265	4.4674262628502327E-4
78-79	9.117196454796393E-6	3.0998467946307735E-4	0.0	0.5658177705828915	4.4674262628502327E-4
80-81	9.117196454796393E-6	3.0998467946307735E-4	0.0	0.5670349163096069	4.6953561742201427E-4
82-83	9.117196454796393E-6	3.0998467946307735E-4	0.0	0.5675819480968947	4.923286085590053E-4
84-85	9.117196454796393E-6	3.1910187591787373E-4	0.0	0.5680058977320427	4.923286085590053E-4
86-87	9.117196454796393E-6	3.1910187591787373E-4	0.0	0.5681563314735468	4.923286085590053E-4
88-89	9.117196454796393E-6	3.282190723726701E-4	0.0	0.5682566206345496	5.151215996959962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11165	0.0	51.440075	1
GTATCAA	19785	0.0	39.603302	1
CTTATAC	16360	0.0	32.435482	1
ATCAACG	24020	0.0	31.968788	3
TCAACGC	24725	0.0	31.110674	4
TATCAAC	26210	0.0	29.71969	2
CAACGCA	25375	0.0	28.815193	5
AACGCAG	26530	0.0	27.726242	6
TATACAC	21335	0.0	26.951084	3
TTATACA	21985	0.0	25.098782	2
ACGCAGA	30155	0.0	24.303421	7
CGCAGAG	31190	0.0	23.545286	8
ACATCTC	24510	0.0	20.42573	8
GCAGAGT	36420	0.0	20.059103	9
GTGGTAT	6265	0.0	19.450138	1
TACATGG	25185	0.0	19.26231	2
TACACAT	27745	0.0	19.03583	5
ACACATC	26570	0.0	18.89399	6
GTACATG	26420	0.0	18.884035	1
ATACACA	32200	0.0	18.794983	4
>>END_MODULE