FastQCFastQC Report
Fri 27 May 2016
SRR2139969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9447680
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103850.051.2398641
GTATCAA188650.039.7942851
CTTATAC127450.037.0278781
ATCAACG220950.033.1150553
TCAACGC225050.032.570624
TATCAAC238350.031.0107442
CAACGCA228800.030.48665
TATACAC169500.029.4764443
AACGCAG239250.029.3730186
TTATACA181250.026.8546312
ACGCAGA269300.026.010067
CGCAGAG272150.025.7161038
GCAGAGT317900.021.6934349
TACACAT229450.020.6188075
ACACATC215250.020.2786666
TACATGG212300.019.5021292
AGAGTAC295150.019.30819910-11
GTACATG222350.019.2663331
GAGTACT207050.019.12470412-13
ATACACA284150.018.9291924
Produced by FastQC (version 0.11.5)