FastQCFastQC Report
Fri 27 May 2016
SRR2139968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8733369
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100800.052.025531
CTTATAC96650.043.839691
GTATCAA183550.040.4542541
ATCAACG218650.033.195143
TATACAC145050.032.604853
TCAACGC224500.032.2657364
TATCAAC237100.031.0630422
CAACGCA228900.030.2924965
TTATACA144900.030.2664892
AACGCAG240050.029.1421746
ACGCAGA269500.025.8276317
CGCAGAG273300.025.4776468
ACATCTC169050.023.3817658
ACACATC175600.022.6049296
GCAGAGT318800.021.6274079
TACACAT202950.021.555265
CATCTCC189800.020.780619
CACATCT195600.020.3875927
ATACACA249850.020.074254
TATGCCG114900.019.78186842-43
Produced by FastQC (version 0.11.5)