##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8733369
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78658270365079	33.0	31.0	34.0	30.0	34.0
2	31.470374834728727	33.0	31.0	34.0	29.0	34.0
3	31.800810317301377	33.0	31.0	34.0	30.0	34.0
4	33.00105594988601	37.0	35.0	37.0	28.0	37.0
5	34.45181292580217	37.0	35.0	37.0	28.0	37.0
6	35.1119454588487	37.0	35.0	37.0	32.0	37.0
7	35.25784253476522	37.0	35.0	37.0	32.0	37.0
8	35.41553231061232	37.0	35.0	37.0	32.0	37.0
9	37.15578810422416	39.0	37.0	39.0	33.0	39.0
10-11	37.04536674220452	39.0	37.0	39.0	33.0	39.0
12-13	36.94346986827191	39.0	37.0	39.0	32.5	39.0
14-15	38.28445294135631	40.0	38.0	41.0	33.0	41.0
16-17	38.28492853101707	40.0	38.0	41.0	33.0	41.0
18-19	38.227965920139184	40.0	38.0	41.0	33.0	41.0
20-21	38.285987955582364	40.0	38.0	41.0	32.5	41.0
22-23	38.32368348533511	40.0	38.0	41.0	33.0	41.0
24-25	38.209205445119835	40.0	38.0	41.0	32.0	41.0
26-27	38.12637976941392	40.0	38.0	41.0	32.0	41.0
28-29	38.13566107056478	40.0	38.0	41.0	32.0	41.0
30-31	38.086343020110675	40.0	37.5	41.0	32.0	41.0
32-33	37.98658066702788	40.0	37.0	41.0	32.0	41.0
34-35	37.87464091893689	40.0	37.0	41.0	31.5	41.0
36-37	37.83161814526305	40.0	37.0	41.0	31.0	41.0
38-39	37.758074718541565	40.0	37.0	41.0	31.0	41.0
40-41	37.69072951064158	40.0	37.0	41.0	31.0	41.0
42-43	37.67965891988028	40.0	37.0	41.0	31.0	41.0
44-45	37.6259929650446	40.0	37.0	41.0	31.0	41.0
46-47	37.502916248495154	40.0	36.5	41.0	30.5	41.0
48-49	37.687732182383016	40.0	37.0	41.0	31.0	41.0
50-51	37.65463332162814	40.0	37.0	41.0	31.0	41.0
52-53	37.606892324537185	40.0	36.5	41.0	31.0	41.0
54-55	37.48769262148482	40.0	36.0	41.0	31.0	41.0
56-57	37.36640879787108	39.0	36.0	41.0	30.5	41.0
58-59	37.270119456657305	39.0	35.5	41.0	31.0	41.0
60-61	37.06004024504047	39.0	35.0	41.0	31.0	41.0
62-63	36.8932595519247	39.0	35.0	41.0	31.0	41.0
64-65	36.65144380622269	38.5	35.0	40.5	30.0	41.0
66-67	36.446091176968736	38.0	35.0	40.0	30.0	41.0
68-69	36.20648619855723	37.5	34.5	40.0	30.0	41.0
70-71	35.97485349659472	37.0	34.0	39.5	30.0	41.0
72-73	35.675880373725164	36.5	34.0	39.0	30.0	41.0
74-75	35.494933599518205	36.0	34.0	39.0	30.0	41.0
76-77	35.28791489634894	36.0	34.0	39.0	30.0	40.0
78-79	35.0162233240491	35.0	34.0	37.5	30.0	39.0
80-81	34.74219224257685	35.0	34.0	37.0	30.0	39.0
82-83	34.47201258595911	35.0	34.0	36.5	30.0	39.0
84-85	34.23847805182032	35.0	34.0	36.0	30.0	37.5
86-87	34.031257138538564	35.0	34.0	36.0	30.0	37.0
88-89	33.875655730232964	35.0	34.0	35.5	30.0	37.0
90-91	33.72441733240815	35.0	34.0	35.0	29.0	36.0
92-93	33.66729772954996	35.0	34.0	35.0	29.5	36.0
94-95	33.63306942152704	35.0	34.0	35.0	30.0	36.0
96-97	33.631115331777735	35.0	34.0	35.0	30.0	36.0
98-99	33.65775825773302	35.0	34.0	35.0	30.0	36.0
100-101	33.26397687029792	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	1.0
16	4.0
17	84.0
18	504.0
19	1897.0
20	5482.0
21	11716.0
22	20133.0
23	30586.0
24	45853.0
25	66902.0
26	96636.0
27	132357.0
28	147803.0
29	128370.0
30	152394.0
31	189269.0
32	237927.0
33	313983.0
34	430093.0
35	605451.0
36	922188.0
37	1587444.0
38	2239254.0
39	1300112.0
40	66924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.18665294000517	21.461156628100795	14.854588189277242	24.497602242616797
2	13.706714284583041	21.223968947429466	42.705401174113604	22.36391559387389
3	16.963951384306874	27.134803450615824	31.402314808347086	24.498930356730213
4	11.464174478229658	19.200795541243224	39.91738109385168	29.417648886675433
5	12.452649877836055	38.18857056619536	36.46595474403511	12.89282481193348
6	28.1209579466025	39.568121920476685	19.357144494098772	12.953775638822046
7	24.848949847249052	33.3820780313739	24.085481432920034	17.683490688457013
8	23.155718441057957	37.71894418774596	21.9252374780625	17.200099893133583
9	24.617924305775976	17.271360307639178	21.89752429423401	36.21319109235084
10-11	23.127910140214887	27.408838450365458	29.855412887351086	19.60783852206857
12-13	24.25741147376039	24.8045375446613	29.664739085210673	21.273311896367638
14-15	21.307131130764354	26.326772763640577	27.20386334587623	25.16223275971884
16-17	20.969013293626578	29.434227933860036	27.899531886818334	21.697226885695056
18-19	20.853863307488517	28.458060317500113	29.74140196344849	20.946674411562878
20-21	21.68799154238886	27.65621205594378	29.556162657273955	21.099633744393405
22-23	21.303647282679727	27.924846294944405	29.389908370904532	21.381598051471336
24-25	21.17381548654865	28.058274195583806	29.62116470228852	21.146745615579025
26-27	21.383455383905396	28.15964995459696	29.443873000056254	21.013021661441396
28-29	21.050957162012356	28.02200282258941	29.703609758861717	21.22343025653652
30-31	21.579828562697063	27.96829333824411	29.430943160122247	21.020934938936577
32-33	21.371400243986294	27.839304444549057	29.466570321354506	21.322724990110142
34-35	21.10060535152728	28.192420154867676	29.79028085522121	20.91669363838384
36-37	21.45808452990268	27.951592603663933	29.39318896606918	21.19713390036421
38-39	21.10729293107055	27.934429993374355	29.626399791615555	21.331877283939537
40-41	21.37602557677255	27.803800429470538	29.590042637339536	21.230131356417374
42-43	21.543984131477988	27.769517762864925	29.35144959274625	21.335048512910834
44-45	21.305651420133113	28.25310941083659	29.08726973810995	21.353969430920348
46-47	21.64631224259901	27.851275435810173	28.636873597044072	21.86553872454674
48-49	21.56988628114877	27.8995391352362	28.718222533231526	21.812352050383506
50-51	21.336282971081637	28.08045698916824	28.77661733689849	21.806642702851637
52-53	21.42526516004427	28.123165099288883	28.924265182592023	21.52730455807483
54-55	21.50738520577064	28.041216039208717	28.638368140527128	21.813030614493517
56-57	21.772702079337655	28.37024205382523	28.22625150512577	21.63080436171135
58-59	21.40017909373697	28.652771296688893	28.723134987907745	21.223914621666385
60-61	21.675755300934565	28.95824532662772	28.0704138208229	21.29558555161482
62-63	21.41014383139154	29.167159774704032	28.001869723326113	21.420826670578315
64-65	21.343287906561777	29.087956517340004	28.058255849781183	21.51049972631704
66-67	21.46547412267659	28.958028223958816	28.045273129245867	21.531224524118727
68-69	21.49878822481522	28.68839288102441	28.21683525870611	21.595983635454264
70-71	21.590452573763557	28.256470396946188	28.338834579753513	21.814242449536742
72-73	21.668890810451437	28.24913752900945	28.39435820688452	21.68761345365459
74-75	21.58237349779806	28.349439530430665	28.345310981717535	21.722875990053737
76-77	21.517157246678728	28.37717404428718	28.3296827260199	21.77598598301419
78-79	21.563860968362345	28.330262353100405	28.30612132183384	21.79975535670341
80-81	21.491407926248456	28.337219621084436	28.340884306526053	21.830488146141057
82-83	21.632605532562764	28.278558734643106	28.455751360069304	21.633084372724827
84-85	21.820577839875906	28.275735654807388	28.243162754506514	21.660523750810196
86-87	21.501330070626576	28.3837301820887	28.36666495692756	21.74827479035716
88-89	21.708363666299416	28.23684921328579	28.30612555158928	21.74866156882551
90-91	21.71178352427183	28.282724870582186	28.35931070467486	21.64618090047112
92-93	21.750783987355412	28.29200137560432	28.32002665522333	21.637187981816933
94-95	21.49192506290929	28.411161946032543	28.35865390405454	21.73825908700363
96-97	21.564322062816196	28.42274193828628	28.395664008671282	21.61727199022624
98-99	21.784254704905802	28.96747806806606	28.469214534249865	20.779052692778276
100-101	22.41379750766425	31.522547905884018	25.673151065148936	20.390503521302797
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1467.0
1	1309.5
2	1949.0
3	2828.0
4	3864.5
5	6623.5
6	8847.5
7	10024.0
8	10370.0
9	9473.5
10	7987.5
11	6734.5
12	7526.0
13	11107.5
14	19930.5
15	32221.0
16	42236.5
17	48004.0
18	49615.0
19	47604.0
20	43688.0
21	40202.0
22	39530.5
23	44231.0
24	54076.5
25	68424.5
26	86859.5
27	106708.0
28	126832.0
29	148204.5
30	169293.0
31	190838.5
32	214570.5
33	237711.5
34	262096.0
35	286994.5
36	310514.0
37	332295.5
38	344653.0
39	349508.0
40	356803.0
41	362142.0
42	365076.0
43	372200.0
44	388404.5
45	407430.0
46	404026.0
47	389150.5
48	372161.5
49	350626.5
50	333171.0
51	314289.5
52	293437.0
53	272901.5
54	251176.5
55	225974.0
56	200696.0
57	174456.5
58	149546.5
59	127858.5
60	104916.0
61	82423.0
62	63140.5
63	47995.5
64	35757.0
65	25991.5
66	19043.5
67	14591.0
68	11521.5
69	8707.5
70	6555.0
71	4866.5
72	3533.5
73	2499.0
74	1702.0
75	1171.5
76	804.0
77	526.5
78	354.0
79	261.0
80	200.5
81	162.5
82	126.5
83	81.0
84	48.0
85	33.5
86	26.0
87	21.5
88	13.5
89	6.5
90	3.5
91	3.0
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6731801896839582
3	0.0063091345390306995
4	8.540243747859504
5	2.6335770308113626E-4
6	0.0729271830836416
7	0.02476707442454338
8	4.1221205699656113E-4
9	2.40457033247994E-4
10-11	0.27302751091818056
12-13	0.28679653865535737
14-15	7.442717695771242E-5
16-17	1.2595368408228257E-4
18-19	0.43225014310056065
20-21	2.5767177631707785E-4
22-23	1.7182358895032282E-4
24-25	0.1638490020124471
26-27	0.14181083279774298
28-29	1.0897130968946504E-4
30-31	5.742251133563441E-5
32-33	1.0358875173482384E-4
34-35	3.63597433002123E-4
36-37	2.259759204694551E-4
38-39	0.0014568051447365047
40-41	0.0017421127604480573
42-43	0.0011813476778868904
44-45	0.0010007822185304478
46-47	0.0013777800774204107
48-49	0.002545109020261194
50-51	3.0028217331980634E-4
52-53	0.0014269744879200122
54-55	5.481058148172186E-4
56-57	0.007918193249287787
58-59	1.8396531657481193E-4
60-61	0.0027354202262096436
62-63	0.0014881989382929956
64-65	0.00151080915275654
66-67	0.00475863589830206
68-69	0.0019909278045335235
70-71	0.0023974486256642734
72-73	0.003495778521208765
74-75	6.106051568936073E-4
76-77	3.225444550401077E-4
78-79	7.302520007486855E-4
80-81	4.446369610928316E-4
82-83	0.0015380689848606853
84-85	2.0546053943224353E-4
86-87	3.7114314538322797E-4
88-89	1.1266875244068685E-4
90-91	3.2706128664541077E-4
92-93	0.0
94-95	0.0
96-97	4.760254917997865E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	864.0
20-21	1987.0
22-23	2759.0
24-25	3485.0
26-27	4720.0
28-29	7952.0
30-31	18135.0
32-33	23186.0
34-35	30660.0
36-37	45281.0
38-39	56109.0
40-41	57593.0
42-43	67409.0
44-45	81798.0
46-47	80619.0
48-49	75032.0
50-51	65588.0
52-53	65345.0
54-55	70003.0
56-57	74184.0
58-59	75676.0
60-61	77939.0
62-63	80662.0
64-65	87603.0
66-67	95200.0
68-69	98407.0
70-71	86423.0
72-73	74406.0
74-75	74506.0
76-77	77202.0
78-79	80469.0
80-81	80981.0
82-83	77955.0
84-85	77727.0
86-87	78395.0
88-89	82350.0
90-91	84955.0
92-93	85648.0
94-95	94009.0
96-97	141937.0
98-99	693995.0
100-101	5494215.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.65340098205652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.05307423545837	74.49592527703646
2	7.218478885520703	12.077006173819438
3	1.991963468330413	4.999035562735562
4	0.8016009085518558	2.682265689226769
5	0.381148835566861	1.5942198187759278
6	0.19509506530376064	0.9792219436485594
7	0.11682509016394341	0.6840971278574486
8	0.07294984907793564	0.48820023811976465
9	0.045855065604996616	0.34523389711019614
>10	0.12140372679222086	1.4546051527734856
>50	0.0010711768672829667	0.05556535913160206
>100	4.4445524353351666E-4	0.07447147633602139
>500	4.4619163311299435E-5	0.02243256338672769
>1k	4.461835480001158E-5	0.047719720041918545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.1450334916571143E-5	0.0	2.2900669833142286E-5	0.0
5	0.0	1.1450334916571143E-5	0.0	2.2900669833142286E-5	0.0
6	0.0	1.1450334916571143E-5	0.0	2.2900669833142286E-5	0.0
7	0.0	1.1450334916571143E-5	0.0	2.2900669833142286E-5	0.0
8	0.0	1.1450334916571143E-5	0.0	2.2900669833142286E-5	0.0
9	0.0	4.580133966628457E-5	0.0	2.2900669833142286E-5	0.0
10-11	0.0	4.580133966628457E-5	0.0	3.435100474971343E-5	0.0
12-13	0.0	4.580133966628457E-5	0.0	5.152650712457014E-5	0.0
14-15	0.0	6.870200949942686E-5	0.0	5.725167458285571E-5	0.0
16-17	0.0	6.870200949942686E-5	0.0	5.725167458285571E-5	0.0
18-19	5.725167458285571E-6	6.870200949942686E-5	0.0	6.297684204114128E-5	0.0
20-21	1.1450334916571143E-5	6.870200949942686E-5	0.0	8.0152344415998E-5	0.0
22-23	1.1450334916571143E-5	9.160267933256914E-5	0.0	9.732784679085472E-5	0.0
24-25	1.1450334916571143E-5	9.160267933256914E-5	0.0	1.1450334916571142E-4	0.0
26-27	1.1450334916571143E-5	9.160267933256914E-5	0.0	1.774801912068527E-4	5.725167458285571E-6
28-29	1.1450334916571143E-5	9.160267933256914E-5	0.0	6.984704299108396E-4	1.1450334916571143E-5
30-31	1.1450334916571143E-5	1.3740401899885372E-4	0.0	0.00275380554743536	1.1450334916571143E-5
32-33	1.1450334916571143E-5	1.3740401899885372E-4	0.0	0.00787210525514266	1.1450334916571143E-5
34-35	1.1450334916571143E-5	1.6602985629028157E-4	0.0	0.015062915582749338	1.1450334916571143E-5
36-37	1.1450334916571143E-5	1.946556935817094E-4	0.0	0.024864402271334234	1.1450334916571143E-5
38-39	1.1450334916571143E-5	2.1183119595656613E-4	0.0	0.03853037699426189	1.1450334916571143E-5
40-41	1.1450334916571143E-5	2.4045703324799398E-4	0.0	0.051715437650693565	1.1450334916571143E-5
42-43	1.1450334916571143E-5	2.4618220070627957E-4	0.0	0.06282226251976757	1.1450334916571143E-5
44-45	1.1450334916571143E-5	2.576325356228507E-4	0.0	0.07511992222016498	1.1450334916571143E-5
46-47	1.1450334916571143E-5	2.6335770308113626E-4	0.0	0.08709697254289839	1.1450334916571143E-5
48-49	1.7175502374856715E-5	2.6335770308113626E-4	0.0	0.09722479377660556	1.1450334916571143E-5
50-51	2.2900669833142286E-5	2.7480803799770744E-4	0.0	0.10611025367186477	1.1450334916571143E-5
52-53	2.2900669833142286E-5	2.7480803799770744E-4	0.0	0.11322091165505546	1.1450334916571143E-5
54-55	2.2900669833142286E-5	2.80533205455993E-4	0.0	0.12042889748503699	1.1450334916571143E-5
56-57	2.2900669833142286E-5	2.8625837291427857E-4	0.0	0.12707009173664824	1.1450334916571143E-5
58-59	2.2900669833142286E-5	2.8625837291427857E-4	0.0	0.13350517995976124	1.1450334916571143E-5
60-61	2.2900669833142286E-5	2.9198354037256413E-4	0.0	0.1396998111496262	1.1450334916571143E-5
62-63	2.2900669833142286E-5	2.977087078308497E-4	0.0	0.14594024367915748	1.1450334916571143E-5
64-65	2.2900669833142286E-5	3.3205971258056313E-4	0.0	0.15182571582627508	1.1450334916571143E-5
66-67	2.2900669833142286E-5	3.3205971258056313E-4	0.0	0.15715584672993893	1.1450334916571143E-5
68-69	2.2900669833142286E-5	3.377848800388487E-4	0.0	0.16259475581531022	1.1450334916571143E-5
70-71	2.2900669833142286E-5	3.492352149554198E-4	0.0	0.16805656557051465	1.1450334916571143E-5
72-73	2.2900669833142286E-5	3.549603824137054E-4	0.0	0.17226456365235454	1.1450334916571143E-5
74-75	2.2900669833142286E-5	3.6641071733027657E-4	0.0	0.17558516077816017	1.1450334916571143E-5
76-77	2.2900669833142286E-5	4.0648688953827557E-4	0.0	0.17860232402867668	1.1450334916571143E-5
78-79	2.2900669833142286E-5	4.2366239191313226E-4	0.0	0.18127025206423775	1.1450334916571143E-5
80-81	2.2900669833142286E-5	4.2366239191313226E-4	0.0	0.18258131541218514	1.1450334916571143E-5
82-83	2.2900669833142286E-5	4.2366239191313226E-4	0.0	0.1830736798135977	1.1450334916571143E-5
84-85	2.2900669833142286E-5	4.293875593714178E-4	0.0	0.18341718986109484	1.1450334916571143E-5
86-87	2.2900669833142286E-5	4.4656306174627457E-4	0.0	0.18352024287534396	1.1450334916571143E-5
88-89	2.2900669833142286E-5	4.923644014125591E-4	0.0	0.1835488687126354	1.1450334916571143E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10080	0.0	52.02553	1
CTTATAC	9665	0.0	43.83969	1
GTATCAA	18355	0.0	40.454254	1
ATCAACG	21865	0.0	33.19514	3
TATACAC	14505	0.0	32.60485	3
TCAACGC	22450	0.0	32.265736	4
TATCAAC	23710	0.0	31.063042	2
CAACGCA	22890	0.0	30.292496	5
TTATACA	14490	0.0	30.266489	2
AACGCAG	24005	0.0	29.142174	6
ACGCAGA	26950	0.0	25.827631	7
CGCAGAG	27330	0.0	25.477646	8
ACATCTC	16905	0.0	23.381765	8
ACACATC	17560	0.0	22.604929	6
GCAGAGT	31880	0.0	21.627407	9
TACACAT	20295	0.0	21.55526	5
CATCTCC	18980	0.0	20.78061	9
CACATCT	19560	0.0	20.387592	7
ATACACA	24985	0.0	20.07425	4
TATGCCG	11490	0.0	19.781868	42-43
>>END_MODULE