FastQCFastQC Report
Fri 27 May 2016
SRR2139967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9881835
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109700.055.538111
GTATCAA204200.040.51141
CTTATAC86100.033.21661
ATCAACG246550.032.9704933
TCAACGC247300.032.8867074
TATCAAC263000.031.1353032
CAACGCA255500.030.1828085
AACGCAG266350.029.0490076
TATACAC127000.026.1580663
ACGCAGA305750.025.2721427
CGCAGAG310850.024.8371568
TTATACA133850.022.5152032
GCAGAGT367200.020.8394059
ACATCTC134200.020.3874268
GTACATG253100.020.0044861
GAGTACT217700.019.76062212-13
TACATGG248350.019.6368792
GTGGTAT53300.019.5754051
ACACATC138400.019.4182076
TACACAT157750.019.3218485
Produced by FastQC (version 0.11.5)