FastQCFastQC Report
Fri 27 May 2016
SRR2139966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7895941
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94200.052.807871
CTTATAC88250.041.7347221
GTATCAA170350.040.9425241
TATACAC123600.033.134623
ATCAACG207750.032.654353
TCAACGC210850.032.259664
TATCAAC222750.030.976272
TTATACA126950.030.4076442
CAACGCA215650.029.9889855
AACGCAG225550.028.9535796
ACGCAGA250500.025.9829857
CGCAGAG254550.025.581038
ACATCTC153600.022.6619958
GCAGAGT298550.021.6384819
ACACATC167300.020.7739726
TACACAT180700.020.5794325
CACATCT170300.020.4188447
CATCTCC170300.020.2382059
GAGTACT194200.019.4513912-13
ATACACA228000.019.4475234
Produced by FastQC (version 0.11.5)