##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7895941
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79958664838048	33.0	31.0	34.0	30.0	34.0
2	31.472673238059908	33.0	31.0	34.0	29.0	34.0
3	31.802065896895634	33.0	31.0	34.0	30.0	34.0
4	33.03954538160809	37.0	35.0	37.0	28.0	37.0
5	34.47514552097084	37.0	35.0	37.0	28.0	37.0
6	35.155044344936215	37.0	35.0	37.0	32.0	37.0
7	35.269158292849454	37.0	35.0	37.0	32.0	37.0
8	35.42229621523261	37.0	35.0	37.0	32.0	37.0
9	37.154224936584505	39.0	37.0	39.0	33.0	39.0
10-11	37.04218490234413	39.0	37.0	39.0	33.0	39.0
12-13	36.942089042965236	39.0	37.0	39.0	32.5	39.0
14-15	38.28073677348906	40.0	38.0	41.0	33.0	41.0
16-17	38.27744400572395	40.0	38.0	41.0	33.0	41.0
18-19	38.221687700047404	40.0	38.0	41.0	33.0	41.0
20-21	38.283521499700825	40.0	38.0	41.0	33.0	41.0
22-23	38.31867180968493	40.0	38.0	41.0	33.0	41.0
24-25	38.20629567025628	40.0	38.0	41.0	32.0	41.0
26-27	38.125027151732425	40.0	38.0	41.0	32.0	41.0
28-29	38.13373953378357	40.0	38.0	41.0	32.0	41.0
30-31	38.08339438165066	40.0	37.5	41.0	32.0	41.0
32-33	37.979782213983846	40.0	37.0	41.0	32.0	41.0
34-35	37.8639591033149	40.0	37.0	41.0	31.5	41.0
36-37	37.82577411201042	40.0	37.0	41.0	31.0	41.0
38-39	37.75389017247338	40.0	37.0	41.0	31.0	41.0
40-41	37.67865823011611	40.0	37.0	41.0	31.0	41.0
42-43	37.655305768662345	40.0	37.0	41.0	31.0	41.0
44-45	37.59443287627563	40.0	37.0	41.0	31.0	41.0
46-47	37.46843355874274	40.0	36.0	41.0	30.0	41.0
48-49	37.65267358307055	40.0	37.0	41.0	31.0	41.0
50-51	37.61045342383558	40.0	36.5	41.0	31.0	41.0
52-53	37.56235736765741	40.0	36.0	41.0	31.0	41.0
54-55	37.43389504728913	39.5	36.0	41.0	31.0	41.0
56-57	37.31415741006629	39.0	36.0	41.0	30.0	41.0
58-59	37.214439859274144	39.0	35.0	41.0	30.5	41.0
60-61	36.997262597802745	39.0	35.0	41.0	30.5	41.0
62-63	36.831222591531095	39.0	35.0	41.0	30.0	41.0
64-65	36.588517310809095	38.0	35.0	40.0	30.0	41.0
66-67	36.38008889475627	38.0	35.0	40.0	30.0	41.0
68-69	36.138509278194114	37.0	34.5	40.0	30.0	41.0
70-71	35.90266326023851	37.0	34.0	39.5	30.0	41.0
72-73	35.60661222737568	36.0	34.0	39.0	30.0	41.0
74-75	35.432955231086886	36.0	34.0	39.0	30.0	41.0
76-77	35.23164834919796	36.0	34.0	38.5	30.0	40.0
78-79	34.9638323362284	35.0	34.0	37.0	30.0	39.0
80-81	34.698286467582605	35.0	34.0	37.0	30.0	39.0
82-83	34.43193056882441	35.0	34.0	36.5	30.0	39.0
84-85	34.203958985484576	35.0	34.0	36.0	30.0	37.5
86-87	34.00306928405854	35.0	34.0	36.0	30.0	37.0
88-89	33.84958281821203	35.0	34.0	35.5	29.5	37.0
90-91	33.70232256698087	35.0	34.0	35.0	29.0	36.0
92-93	33.64861264581709	35.0	34.0	35.0	29.0	36.0
94-95	33.61666838694924	35.0	34.0	35.0	30.0	36.0
96-97	33.61860628785034	35.0	34.0	35.0	30.0	36.0
98-99	33.64360778928124	35.0	34.0	35.0	30.0	35.5
100-101	33.24743703434157	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	65.0
18	425.0
19	1659.0
20	4603.0
21	9887.0
22	16950.0
23	26634.0
24	40252.0
25	59789.0
26	87379.0
27	121169.0
28	136101.0
29	117774.0
30	140206.0
31	174263.0
32	219496.0
33	289217.0
34	396753.0
35	559642.0
36	851919.0
37	1458673.0
38	2004950.0
39	1124644.0
40	53486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.45639918028769	21.241800059042994	14.786128721073272	24.51567203959604
2	13.789236715249597	21.034498301937095	42.666274294148025	22.509990688665287
3	17.011819742904567	26.923269829118958	31.266729605997597	24.798180821978878
4	11.544387417300962	19.06551262755744	39.55364782890308	29.836452126238516
5	12.540481365242314	38.153453234120875	36.277433007464495	13.028632393172318
6	28.46354895423962	39.30475793597684	19.207773647730637	13.02391946205291
7	25.207045468105743	33.22919990641039	23.787265302337623	17.776489323146237
8	23.31566930466156	37.6880090619228	21.69310487372174	17.303216759693896
9	24.672017361875834	17.251958321264866	21.722405783948446	36.35361853291085
10-11	23.3471059555548	27.28090390190996	29.64682497679041	19.725165165744833
12-13	24.446427913699402	24.63809200841821	29.51790901274035	21.39757106514204
14-15	21.432990066094114	26.16631020698935	27.056755451775043	25.34394427514149
16-17	21.128981711399526	29.360976437289622	27.730142474043113	21.779899377267743
18-19	21.04732100815881	28.298864919550244	29.58065415494493	21.073159917346015
20-21	21.811395789565818	27.536277043709028	29.411922294922928	21.24040487180223
22-23	21.427702632333144	27.726274450776884	29.285691298813816	21.56033161807616
24-25	21.34480873460222	27.779915068760435	29.444302299254687	21.430973897382653
26-27	21.355987340813407	27.849569277455128	29.416355984483864	21.378087397247594
28-29	20.994893999210458	27.86744456805545	29.625836317051508	21.51182511568258
30-31	21.448102624168307	27.660095263675316	29.219281046768653	21.672521065387727
32-33	21.544264975979914	27.730868121176123	29.396454591031635	21.32841231181233
34-35	21.49984504625382	28.079329170259705	29.311970904434638	21.108854879051837
36-37	21.346888450734795	27.843003005873186	29.21968434071585	21.59042420267617
38-39	21.221111039762654	27.790859163317887	29.43576458607049	21.55226521084897
40-41	21.466590968303905	27.680759005707188	29.418191975714308	21.434458050274603
42-43	21.719114503101675	27.624399499003793	29.184751255801306	21.471734742093226
44-45	21.46304961078843	28.12825374604339	28.914121063010445	21.494575580157736
46-47	21.781108365664775	27.73514971348944	28.496181861763993	21.987560059081787
48-49	21.75015939112123	27.74516792703658	28.517814628829356	21.98685805301284
50-51	21.434741877652456	27.925243742870805	28.68134835018673	21.95866602929001
52-53	21.585127491922222	27.92764661050391	28.83605233429712	21.651173563276753
54-55	21.670211713104113	27.900950033123145	28.52125371533738	21.907584538435366
56-57	21.948618563660332	28.18950432074942	28.0839236582299	21.77795345736035
58-59	21.499961557014853	28.54764913820037	28.54702818980146	21.405361114983318
60-61	21.822344263020014	28.865156247491452	27.892888920309794	21.41961056917874
62-63	21.523000886203747	29.09139556369262	27.83401269460034	21.551590855503296
64-65	21.470759685219374	28.966581256004257	27.96576908987194	21.596889968904428
66-67	21.589804286257767	28.828284298494506	27.913622915821435	21.668288499426293
68-69	21.64913539425331	28.56447438160271	28.088935707559408	21.697454516584568
70-71	21.700888475500207	28.097830193279062	28.223558911795237	21.977722419425497
72-73	21.75124027753326	28.137627545070565	28.29128352360703	21.819848653789148
74-75	21.70764847368275	28.226526298261135	28.225181685985785	21.840643542070335
76-77	21.67765635661742	28.244303623245	28.14103025408732	21.93700976605026
78-79	21.671903890513946	28.199424842187458	28.196742333315996	21.931928933982604
80-81	21.65541895077118	28.23605375705999	28.191257252002444	21.917270040166382
82-83	21.767682666225912	28.163011142077615	28.291441925341378	21.777864266355095
84-85	21.945806452026268	28.13692579774151	28.138495548075927	21.778772202156294
86-87	21.62317920166994	28.25186438754032	28.229056715321143	21.895899695468593
88-89	21.801606965142355	28.127482582313036	28.175572315314707	21.895338137229903
90-91	21.839952659689644	28.202943488706083	28.16038295201904	21.796720899585225
92-93	21.79875506069138	28.18874318990275	28.213713126971125	21.79878862243475
94-95	21.60316407789781	28.269427325085783	28.21046122235082	21.916947374665586
96-97	21.595284003671654	28.28147323890043	28.290843933752118	21.832398823675796
98-99	21.872223842236252	28.82899635550381	28.305601945063795	20.99317785719614
100-101	22.481427369550357	31.46499322535882	25.54088804564616	20.512691359444663
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1604.0
1	1382.0
2	1873.5
3	2643.0
4	3486.0
5	5739.0
6	7583.5
7	8796.5
8	9339.5
9	8640.5
10	7314.5
11	6252.0
12	7081.0
13	10200.5
14	17757.0
15	28442.0
16	37350.5
17	42332.0
18	43551.5
19	41961.0
20	38605.0
21	35481.0
22	35024.0
23	39105.0
24	47701.5
25	60252.5
26	75990.0
27	93581.0
28	112764.5
29	133324.5
30	152355.5
31	170328.5
32	190824.5
33	211034.5
34	230795.0
35	250690.5
36	270061.5
37	288007.5
38	298542.5
39	303115.5
40	310291.5
41	316211.0
42	318054.0
43	320951.0
44	324888.5
45	332537.5
46	346824.5
47	353031.5
48	344886.5
49	327864.0
50	306921.0
51	288475.0
52	270176.5
53	253038.5
54	234922.5
55	213775.0
56	191822.5
57	168397.0
58	146396.5
59	126277.0
60	104283.0
61	82974.5
62	64739.0
63	50115.5
64	38035.0
65	28053.0
66	20840.5
67	15704.5
68	11877.0
69	8878.5
70	6875.0
71	5279.0
72	3809.5
73	2696.0
74	1872.5
75	1282.5
76	880.0
77	580.5
78	393.0
79	277.0
80	200.0
81	144.5
82	108.5
83	79.5
84	57.0
85	45.0
86	35.0
87	25.5
88	16.5
89	10.0
90	7.5
91	7.5
92	6.5
93	4.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6617525384244893
3	0.006421020623127756
4	8.413145437636881
5	3.039536389646275E-4
6	0.0734174685449144
7	0.02408832588794673
8	5.699130730586765E-4
9	2.532946991371896E-4
10-11	0.272323463409871
12-13	0.28534914331300093
14-15	4.432657234900818E-5
16-17	1.45644452003884E-4
18-19	0.4276501052882741
20-21	2.216673080240779E-4
22-23	1.520391105407955E-4
24-25	0.16094398842485919
26-27	0.14078856558723485
28-29	1.0149949646734174E-4
30-31	1.2702315333580904E-4
32-33	1.336586627616273E-4
34-35	3.446241127365031E-4
36-37	2.3702031891916685E-4
38-39	0.001346084684055247
40-41	0.0017498459163456995
42-43	0.0010959742712211443
44-45	8.417376121048182E-4
46-47	0.0013408791640189281
48-49	0.0022305551356053602
50-51	3.174714680961439E-4
52-53	0.001633429329067542
54-55	4.869514166514066E-4
56-57	0.008188487926544839
58-59	2.0930801097108868E-4
60-61	0.002506724499711304
62-63	0.001336360616628065
64-65	0.0013788464699699089
66-67	0.004422984616946655
68-69	0.0017201399870504334
70-71	0.0023001350156922804
72-73	0.0033103066397227768
74-75	6.305204247215967E-4
76-77	2.685207796339724E-4
78-79	6.512406871612628E-4
80-81	4.545460957546805E-4
82-83	0.001545043851910003
84-85	1.6017834898088927E-4
86-87	3.724348649879084E-4
88-89	1.3920046365217963E-4
90-91	2.2374063852595026E-4
92-93	0.0
94-95	0.0
96-97	5.1771767957555433E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	788.0
20-21	1887.0
22-23	2513.0
24-25	3147.0
26-27	4307.0
28-29	7027.0
30-31	15820.0
32-33	19897.0
34-35	26404.0
36-37	38828.0
38-39	48392.0
40-41	49206.0
42-43	57435.0
44-45	69710.0
46-47	69600.0
48-49	64345.0
50-51	56563.0
52-53	55354.0
54-55	59448.0
56-57	62922.0
58-59	64385.0
60-61	66783.0
62-63	70132.0
64-65	75824.0
66-67	81934.0
68-69	86018.0
70-71	75176.0
72-73	64419.0
74-75	64057.0
76-77	66896.0
78-79	69392.0
80-81	69823.0
82-83	67754.0
84-85	67178.0
86-87	68570.0
88-89	71539.0
90-91	74853.0
92-93	74869.0
94-95	83204.0
96-97	127898.0
98-99	636104.0
100-101	5055540.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.3084851328132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.91986610775562	74.077793436497
2	7.315318087922264	12.188561363389427
3	2.0512340019358017	5.126555920625689
4	0.789319210839202	2.6302795096496605
5	0.3599953890592158	1.4995335258660492
6	0.1947929003964849	0.9736740867994874
7	0.1099234448419494	0.6410288969254208
8	0.0762412752784586	0.508123611843375
9	0.044899455592998114	0.3366455065866621
>10	0.13549466283984973	1.654241225595638
>50	0.0018972407501671733	0.1021415099501644
>100	8.36269686597467E-4	0.15063647165410562
>500	1.5595991054058561E-4	0.08090167839101114
>1k	2.599319080501045E-5	0.029883256226323544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.266473495685948E-5	0.0	0.0	0.0
3	0.0	1.266473495685948E-5	0.0	1.266473495685948E-5	0.0
4	0.0	3.799420487057844E-5	0.0	1.266473495685948E-5	0.0
5	0.0	3.799420487057844E-5	0.0	1.266473495685948E-5	0.0
6	0.0	3.799420487057844E-5	0.0	1.266473495685948E-5	0.0
7	0.0	3.799420487057844E-5	0.0	1.266473495685948E-5	0.0
8	0.0	3.799420487057844E-5	0.0	1.266473495685948E-5	0.0
9	0.0	6.33236747842974E-5	0.0	6.33236747842974E-5	0.0
10-11	0.0	6.33236747842974E-5	0.0	6.33236747842974E-5	0.0
12-13	0.0	6.33236747842974E-5	0.0	7.598840974115689E-5	0.0
14-15	0.0	8.232077721958662E-5	0.0	1.0765024713330559E-4	0.0
16-17	0.0	8.865314469801637E-5	0.0	1.4564445200388403E-4	0.0
18-19	0.0	9.49855121764461E-5	0.0	1.773062893960327E-4	0.0
20-21	0.0	1.0131787965487584E-4	0.0	2.2163286174504092E-4	0.0
22-23	0.0	1.0765024713330559E-4	0.0	2.532946991371896E-4	0.0
24-25	0.0	1.2031498209016505E-4	0.0	2.849565365293383E-4	0.0
26-27	0.0	1.393120845254543E-4	0.0	3.7360968122735464E-4	0.0
28-29	0.0	1.4564445200388403E-4	0.0	8.675343445448744E-4	0.0
30-31	0.0	1.7730628939603273E-4	0.0	0.0027102532807679286	0.0
32-33	0.0	1.899710243528922E-4	0.0	0.007776147263511721	0.0
34-35	0.0	2.216328617450409E-4	0.0	0.014672095447521707	0.0
36-37	0.0	2.7229180157247883E-4	0.0	0.023885690128636977	0.0
38-39	0.0	3.1028600644305725E-4	0.0	0.03651876324810431	0.0
40-41	0.0	3.482802113136357E-4	0.0	0.04817031940841503	0.0
42-43	0.0	3.672773137489249E-4	0.0	0.0582767779039889	0.0
44-45	0.0	3.7360968122735464E-4	0.0	0.06926976784654293	0.0
46-47	0.0	3.799420487057844E-4	0.0	0.07983848916804216	0.0
48-49	0.0	3.799420487057844E-4	0.0	0.08919139593368289	0.0
50-51	0.0	4.0527151861950337E-4	0.0	0.0971385171191122	0.0
52-53	0.0	4.1793625357636286E-4	0.0	0.10415478028521237	0.0
54-55	0.0	4.3693335601165204E-4	0.0	0.11082276323999887	0.0
56-57	0.0	4.432657234900818E-4	0.0	0.11744008725495797	0.0
58-59	0.0	4.432657234900818E-4	0.0	0.12362681028138381	0.0
60-61	0.0	4.432657234900818E-4	0.0	0.1293259410119706	0.0
62-63	0.0	4.432657234900818E-4	0.0	0.13449315287436925	0.0
64-65	0.0	5.065893982743792E-4	0.0	0.1399326565383404	0.0
66-67	0.0	5.065893982743792E-4	0.0	0.14547981044944486	0.0
68-69	0.0	5.319188681880982E-4	0.0	0.15090031701098072	0.0
70-71	0.0	5.38251235666528E-4	0.0	0.15592821678885393	0.0
72-73	0.0	5.445836031449577E-4	0.0	0.16000626144496266	0.0
74-75	0.0	5.509159706233873E-4	0.0	0.16319777465409127	0.0
76-77	0.0	5.572483381018171E-4	0.0	0.1661423255315611	0.0
78-79	0.0	5.63580705580247E-4	0.0	0.16868793725788983	0.0
80-81	0.0	5.762454405371063E-4	0.0	0.16992908128366208	0.0
82-83	0.0	5.889101754939658E-4	0.0	0.1703660146396737	0.0
84-85	0.0	6.07907277929255E-4	0.0	0.17079661562820694	0.0
86-87	0.0	6.07907277929255E-4	0.0	0.17089160114038338	0.0
88-89	0.0	6.205720128861145E-4	0.0	0.17091059824281868	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9420	0.0	52.80787	1
CTTATAC	8825	0.0	41.734722	1
GTATCAA	17035	0.0	40.942524	1
TATACAC	12360	0.0	33.13462	3
ATCAACG	20775	0.0	32.65435	3
TCAACGC	21085	0.0	32.25966	4
TATCAAC	22275	0.0	30.97627	2
TTATACA	12695	0.0	30.407644	2
CAACGCA	21565	0.0	29.988985	5
AACGCAG	22555	0.0	28.953579	6
ACGCAGA	25050	0.0	25.982985	7
CGCAGAG	25455	0.0	25.58103	8
ACATCTC	15360	0.0	22.661995	8
GCAGAGT	29855	0.0	21.638481	9
ACACATC	16730	0.0	20.773972	6
TACACAT	18070	0.0	20.579432	5
CACATCT	17030	0.0	20.418844	7
CATCTCC	17030	0.0	20.238205	9
GAGTACT	19420	0.0	19.45139	12-13
ATACACA	22800	0.0	19.447523	4
>>END_MODULE