FastQCFastQC Report
Fri 27 May 2016
SRR2139965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14453804
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162300.054.058271
GTATCAA300300.040.5268361
ATCAACG362150.032.7774663
TCAACGC365350.032.55154
TATCAAC386200.030.9735222
CAACGCA380800.029.7280145
CTTATAC129050.029.224151
AACGCAG396350.028.7343756
ACGCAGA452950.025.0577727
CGCAGAG457750.024.8170688
TATACAC189400.023.2926353
GTGGTAT79900.022.7608261
TGGTATC79800.021.2236712
GCAGAGT540950.020.7873559
TTATACA199550.020.3371522
GAGTACT332250.019.42929812-13
GTACATG354600.019.1701431
ACATCTC190850.018.9849018
AGAGTAC486000.018.95224810-11
TACATGG347600.018.8373152
Produced by FastQC (version 0.11.5)