##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14453804
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744026209294105	33.0	31.0	34.0	30.0	34.0
2	31.46204715381501	33.0	31.0	34.0	28.0	34.0
3	31.772384626220198	33.0	31.0	34.0	30.0	34.0
4	33.067276268586454	37.0	35.0	37.0	28.0	37.0
5	34.471241134859724	37.0	35.0	37.0	28.0	37.0
6	35.07745096031467	37.0	35.0	37.0	32.0	37.0
7	35.20075704638032	37.0	35.0	37.0	32.0	37.0
8	35.35300333393202	37.0	35.0	37.0	32.0	37.0
9	37.099059112742914	39.0	37.0	39.0	32.0	39.0
10-11	36.991655380133835	39.0	37.0	39.0	33.0	39.0
12-13	36.88854965101228	39.0	37.0	39.0	32.0	39.0
14-15	38.22589188285659	40.0	38.0	41.0	32.0	41.0
16-17	38.22431869838556	40.0	38.0	41.0	32.0	41.0
18-19	38.17518512773523	40.0	38.0	41.0	32.5	41.0
20-21	38.226903780629314	40.0	38.0	41.0	32.0	41.0
22-23	38.26719646172599	40.0	38.0	41.0	32.0	41.0
24-25	38.15938959625343	40.0	38.0	41.0	32.0	41.0
26-27	38.07568707634947	40.0	38.0	41.0	32.0	41.0
28-29	38.08300808902149	40.0	38.0	41.0	32.0	41.0
30-31	38.03317203219967	40.0	37.5	41.0	32.0	41.0
32-33	37.93569879793566	40.0	37.0	41.0	32.0	41.0
34-35	37.83016082924189	40.0	37.0	41.0	31.0	41.0
36-37	37.79363464317012	40.0	37.0	41.0	31.0	41.0
38-39	37.730094250374606	40.0	37.0	41.0	31.0	41.0
40-41	37.64335299372978	40.0	37.0	41.0	31.0	41.0
42-43	37.61833397434728	40.0	37.0	41.0	31.0	41.0
44-45	37.56407594693215	40.0	37.0	41.0	31.0	41.0
46-47	37.43553129257228	40.0	36.0	41.0	30.0	41.0
48-49	37.621104841503055	40.0	37.0	41.0	31.0	41.0
50-51	37.586875970215594	40.0	36.5	41.0	31.0	41.0
52-53	37.52311987771784	40.0	36.0	41.0	31.0	41.0
54-55	37.40324687732556	39.5	36.0	41.0	30.5	41.0
56-57	37.278148419501804	39.0	36.0	41.0	30.0	41.0
58-59	37.16851244505877	39.0	35.0	41.0	30.5	41.0
60-61	36.96320022149325	39.0	35.0	41.0	30.5	41.0
62-63	36.78152800558034	39.0	35.0	41.0	30.0	41.0
64-65	36.530299250982296	38.0	35.0	40.0	30.0	41.0
66-67	36.31762094525702	38.0	35.0	40.0	30.0	41.0
68-69	36.06896239651016	37.0	34.5	40.0	30.0	41.0
70-71	35.82436928953683	37.0	34.0	39.5	30.0	41.0
72-73	35.525492731036906	36.0	34.0	39.0	30.0	41.0
74-75	35.344395669878054	36.0	34.0	39.0	30.0	40.5
76-77	35.14974610768725	35.5	34.0	38.5	30.0	40.0
78-79	34.889576444619415	35.0	34.0	37.0	30.0	39.0
80-81	34.629712560017936	35.0	34.0	37.0	30.0	39.0
82-83	34.377839616154134	35.0	34.0	36.5	30.0	39.0
84-85	34.16048618325435	35.0	34.0	36.0	30.0	37.0
86-87	33.96856004895695	35.0	34.0	36.0	30.0	37.0
88-89	33.823472530840235	35.0	34.0	35.0	29.5	37.0
90-91	33.688041887733235	35.0	34.0	35.0	29.0	36.0
92-93	33.63835470396016	35.0	34.0	35.0	29.5	36.0
94-95	33.609646981642506	35.0	34.0	35.0	30.0	36.0
96-97	33.61428273814086	35.0	34.0	35.0	30.0	36.0
98-99	33.643295618905285	35.0	34.0	35.0	30.0	35.5
100-101	33.25178927953141	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	19.0
17	241.0
18	1232.0
19	4852.0
20	13389.0
21	27847.0
22	44854.0
23	63965.0
24	88567.0
25	122968.0
26	167778.0
27	217998.0
28	233380.0
29	215165.0
30	258092.0
31	317060.0
32	399528.0
33	521640.0
34	712186.0
35	1005830.0
36	1538446.0
37	2668909.0
38	3656234.0
39	2067143.0
40	106478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.127643906060996	20.751582074864167	14.775432128455595	24.345341890619245
2	14.093803183030177	21.13690036721321	42.02721044328799	22.742086006468618
3	17.427157592615284	26.483097243116756	31.02073296261596	25.069012201652
4	12.024228523508597	18.63475057814088	39.16099896284435	30.18002193550617
5	12.974846197449397	37.76442515749709	35.91905053903279	13.341678106020725
6	29.123689659534246	38.7040338411542	18.971038764275562	13.201237735035997
7	25.597343975295473	33.06690828283209	23.590972970563996	17.744774771308442
8	23.640897728005474	37.48978464523903	21.497582769626558	17.37173485712894
9	25.004951996936438	17.21595722636974	21.558265268599566	36.22082550809426
10-11	23.67052050688459	27.11316458106548	29.37216227507391	19.84415263697602
12-13	24.575945171957862	24.598478916726297	29.410755062218254	21.414820849097588
14-15	21.72841457900849	26.04634976488874	26.92888269136237	25.296352964740404
16-17	21.32075983413619	29.270500476484155	27.58720794676218	21.82153174261747
18-19	21.3735060250527	28.282206489816925	29.35542122742288	20.988866257707496
20-21	22.073491446624267	27.484273920192344	29.22501669722274	21.217217935960655
22-23	21.80238604120863	27.506814439741827	29.118671331263574	21.572128187785964
24-25	21.668949391481075	27.61834732295577	29.191974370966378	21.520728914596777
26-27	21.69275919196692	27.76185369070683	29.082769462456685	21.462617654869568
28-29	21.541372497914534	27.80315146855739	29.126455832409253	21.52902020111883
30-31	22.028429726406696	27.556195258939802	29.05306924150403	21.362305773149476
32-33	21.512195578535398	27.870722799936853	29.02455379099717	21.592527830530578
34-35	21.75929255982716	27.80747606765256	29.036617769799598	21.396613602720677
36-37	21.66540180294075	27.785197330306914	29.014778753289473	21.534622113462866
38-39	21.55442193658471	27.689230541434284	29.152069070064236	21.604278451916773
40-41	21.805526020895304	27.591513834855053	28.990323451611722	21.612636692637924
42-43	21.811631004389987	27.631085727731048	28.870878796790333	21.68640447108863
44-45	21.73425215231748	27.83334544847385	28.673304898732145	21.759097500476525
46-47	21.98641263957027	27.69153684122258	28.311231234798623	22.010819284408527
48-49	21.855438414662107	27.805423071089535	28.328743243831116	22.01039527041724
50-51	21.7775178680523	27.925365135727215	28.341042777686226	21.956074218534262
52-53	21.928164564294324	27.885798466739363	28.346972531509422	21.839064437456884
54-55	21.87779343037744	27.807220768694762	28.273237638305716	22.04174816262208
56-57	22.002762239205047	27.976185337389282	28.084138194108633	21.936914229297038
58-59	21.818672571247458	28.157708977603075	28.241263351969444	21.782355099180027
60-61	22.01469430630241	28.25800810919591	28.008656365174332	21.718641219327353
62-63	21.856815455899014	28.458000792083176	27.900424992730883	21.784758759286923
64-65	21.84684318405553	28.330668569769617	27.930299170902213	21.892189075272636
66-67	21.945309164280552	28.30101596312293	27.865802096196106	21.887872776400414
68-69	21.893720764127146	28.183322740559785	27.98263076570493	21.940325729608144
70-71	21.92421217203117	27.883589302395528	28.085425099108225	22.106773426465082
72-73	22.016112183183758	27.93182965548996	27.985735202235684	22.066322959090602
74-75	21.854341669395385	28.039708111908713	28.012978310561863	22.092971908134036
76-77	22.006682567063667	27.992729312876907	27.938960753775287	22.061627366284135
78-79	21.966531565772094	28.001307328615116	27.914744097313726	22.11741700829906
80-81	21.962788264383445	28.00620635816132	27.924749314562707	22.10625606289253
82-83	21.9736096506838	28.01873374011142	27.987940807641486	22.019715801563297
84-85	22.085016535656703	27.975101843122058	27.93482642967745	22.005055191543786
86-87	21.938872542510637	27.993020662267558	28.002689024440286	22.06541777078152
88-89	21.96460382572011	28.015267948243157	27.95090029159034	22.069227934446392
90-91	22.022390622289635	27.9972613649012	27.986802066536676	21.99354594627249
92-93	22.031557791201532	27.981167613023977	27.94567717575877	22.04159742001572
94-95	21.88281540677277	28.06577209694636	27.98115604123637	22.070256455044497
96-97	21.918180407141705	28.06406160505141	28.000651190212334	22.01710679759455
98-99	22.16423724405294	28.619533923348982	28.06428607135783	21.151942761240246
100-101	22.787736448180958	31.21097196276766	25.2621453754004	20.739146213650983
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4064.0
1	3294.5
2	3607.0
3	4802.0
4	6068.5
5	9545.5
6	12599.0
7	15020.0
8	16684.5
9	16160.5
10	14160.0
11	12410.0
12	13558.0
13	18457.0
14	30907.5
15	48461.0
16	62926.5
17	71590.0
18	74457.5
19	72015.0
20	66945.0
21	62706.5
22	62947.5
23	70814.5
24	86023.5
25	108966.0
26	138577.0
27	171422.5
28	206080.0
29	240834.0
30	273411.5
31	308087.5
32	344509.5
33	378285.5
34	411830.5
35	442761.5
36	474952.5
37	503815.5
38	522637.0
39	537523.0
40	556308.0
41	575586.5
42	587702.5
43	598784.0
44	614132.5
45	630124.5
46	627381.0
47	614859.0
48	601239.5
49	580109.5
50	560582.0
51	538860.5
52	510809.5
53	479997.5
54	447987.0
55	413870.5
56	378709.5
57	341843.5
58	303298.0
59	263023.0
60	220704.0
61	180056.0
62	143686.0
63	112022.5
64	84860.0
65	63641.5
66	47789.5
67	36368.5
68	27901.5
69	20633.5
70	15097.0
71	11098.0
72	8043.0
73	5587.0
74	3823.0
75	2645.5
76	1854.5
77	1234.5
78	835.0
79	590.0
80	426.0
81	319.5
82	241.0
83	161.5
84	112.5
85	77.0
86	56.0
87	48.0
88	33.5
89	24.0
90	20.5
91	17.5
92	14.0
93	7.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6151803359171053
3	0.0063996993455840406
4	8.324043967940895
5	3.113367249203047E-4
6	0.07143448188449214
7	0.023080429207425257
8	4.981387598724875E-4
9	2.698251615975974E-4
10-11	0.26357421202058645
12-13	0.27581666390384146
14-15	6.226734498406094E-5
16-17	1.2453468996812187E-4
18-19	0.4147454884541122
20-21	1.5224093290198864E-4
22-23	1.349854817922583E-4
24-25	0.15365412713946708
26-27	0.13785853074186688
28-29	9.707100043383803E-5
30-31	1.2149767335426889E-4
32-33	9.74271609318198E-5
34-35	2.7222049525074703E-4
36-37	3.014074606898081E-4
38-39	0.0013114397168531144
40-41	0.0016076218166559484
42-43	0.001107839252810311
44-45	8.360998809242405E-4
46-47	0.001350245000317544
48-49	0.002424931113585684
50-51	2.928238475148355E-4
52-53	0.0014985578156124387
54-55	3.6553164693863476E-4
56-57	0.007674199284960855
58-59	1.612571484430027E-4
60-61	0.0026368907924310533
62-63	0.001468684877288637
64-65	0.0013809334635393998
66-67	0.004516770199654445
68-69	0.002064273210687981
70-71	0.0024742902197411407
72-73	0.0035703797649762315
74-75	6.906336928284012E-4
76-77	4.0195094294517917E-4
78-79	8.644693510522732E-4
80-81	4.1568380180248294E-4
82-83	0.0015028197367287655
84-85	2.3490611844010815E-4
86-87	3.9462547015925155E-4
88-89	9.98580517793956E-5
90-91	2.666539285778486E-4
92-93	0.0
94-95	0.0
96-97	3.839563456993194E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1916.0
20-21	4569.0
22-23	5779.0
24-25	6865.0
26-27	9177.0
28-29	14440.0
30-31	32009.0
32-33	40293.0
34-35	54086.0
36-37	78379.0
38-39	93780.0
40-41	95418.0
42-43	110661.0
44-45	134074.0
46-47	132448.0
48-49	122575.0
50-51	110922.0
52-53	109512.0
54-55	117709.0
56-57	124974.0
58-59	127574.0
60-61	128854.0
62-63	128069.0
64-65	136063.0
66-67	143561.0
68-69	148522.0
70-71	138295.0
72-73	126275.0
74-75	127280.0
76-77	131832.0
78-79	136158.0
80-81	136953.0
82-83	131145.0
84-85	130727.0
86-87	132836.0
88-89	139060.0
90-91	143073.0
92-93	144493.0
94-95	158368.0
96-97	240325.0
98-99	1155463.0
100-101	9069292.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.20941239684518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8976769200587	76.60128226199632
2	7.046716272103767	12.008931057464988
3	1.711892574656063	4.376080811188967
4	0.6116348085809653	2.0846817056256373
5	0.2892883481318248	1.2325045078783385
6	0.1435462250481928	0.7338893692885086
7	0.08594437767196136	0.5126288944168258
8	0.05573394358703343	0.37992452668880294
9	0.03488888329560813	0.2675575120320789
>10	0.11938512702409283	1.5155699025896066
>50	0.0025237486228974182	0.140238033500791
>100	7.154386487802424E-4	0.10850711372548574
>500	4.000428490860432E-5	0.022929201193868327
>1k	1.3328285156496523E-5	0.01527510240965381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.918593887117883E-6
2	0.0	3.459296943558941E-5	0.0	2.075578166135365E-5	6.918593887117883E-6
3	0.0	3.459296943558941E-5	0.0	3.459296943558941E-5	6.918593887117883E-6
4	0.0	7.610453275829671E-5	0.0	6.226734498406095E-5	6.918593887117883E-6
5	0.0	7.610453275829671E-5	0.0	6.226734498406095E-5	6.918593887117883E-6
6	0.0	7.610453275829671E-5	0.0	6.226734498406095E-5	6.918593887117883E-6
7	0.0	8.994172053253247E-5	0.0	6.226734498406095E-5	6.918593887117883E-6
8	0.0	1.0377890830676824E-4	0.0	7.610453275829671E-5	1.3837187774235766E-5
9	6.918593887117883E-6	2.006392227264186E-4	0.0	8.30231266454146E-5	1.3837187774235766E-5
10-11	2.075578166135365E-5	2.1101711355709542E-4	0.0	1.1415679913744507E-4	1.3837187774235766E-5
12-13	3.459296943558941E-5	2.2139500438777225E-4	0.0	1.3837187774235765E-4	1.3837187774235766E-5
14-15	4.151156332270729E-5	2.802030524282742E-4	0.0	1.6604625329082916E-4	1.3837187774235766E-5
16-17	4.843015720982518E-5	2.9404024020251E-4	0.0	1.7988344106506494E-4	2.7674375548471532E-5
18-19	6.226734498406095E-5	3.217146157509815E-4	0.0	1.7988344106506494E-4	3.459296943558942E-5
20-21	7.264523581473777E-5	3.701447729608067E-4	0.0	2.040985196699775E-4	4.4970860266266234E-5
22-23	8.30231266454146E-5	4.635457904368981E-4	0.0	2.6636586465403843E-4	5.8808048040502E-5
24-25	1.003196113632093E-4	4.946794629289285E-4	0.0	2.9749953714606895E-4	6.918593887117882E-5
26-27	1.0377890830676824E-4	5.119759476467233E-4	0.0	3.943598515657193E-4	6.918593887117882E-5
28-29	1.0377890830676824E-4	5.119759476467233E-4	0.0	0.001006655410575652	7.610453275829671E-5
30-31	1.1069750219388613E-4	5.707839956872253E-4	0.0	0.0034523783496718235	7.610453275829671E-5
32-33	1.3837187774235765E-4	5.846211834614611E-4	0.0	0.009644519878642328	7.610453275829671E-5
34-35	1.418311746859166E-4	6.745629039939936E-4	0.0	0.01865252911966981	8.30231266454146E-5
36-37	1.4529047162947553E-4	7.679639214700849E-4	0.0	0.031140591085917588	8.648242358897352E-5
38-39	1.4529047162947553E-4	7.990975939621155E-4	0.0	0.047364693751209025	9.686031441965035E-5
40-41	1.5220906551659342E-4	8.994172053253248E-4	0.0	0.062886559136958	9.686031441965035E-5
42-43	1.5220906551659342E-4	9.582252533658268E-4	0.0	0.07758165255319638	1.003196113632093E-4
44-45	1.5566836246015236E-4	0.0010896785372210664	0.0	0.09343215114858344	1.0377890830676824E-4
46-47	1.591276594037113E-4	0.0011830795546971579	0.0	0.10856657527665381	1.0377890830676824E-4
48-49	1.591276594037113E-4	0.0011969167424713935	0.0	0.12189178710324286	1.0377890830676824E-4
50-51	1.591276594037113E-4	0.0013837187774235766	0.0	0.13328325193838245	1.0377890830676824E-4
52-53	1.660462532908292E-4	0.0014113931529720482	0.0	0.14313532963363831	1.1069750219388613E-4
54-55	1.7296484717794708E-4	0.0014252303407462838	0.0	0.1526241811498205	1.17616096081004E-4
56-57	1.7296484717794708E-4	0.0014598233101818733	0.0	0.16003399520292372	1.2799398691168082E-4
58-59	1.7296484717794708E-4	0.0014805790918432267	0.0	0.16598398594584512	1.3837187774235765E-4
60-61	1.7988344106506494E-4	0.001525549952109493	0.0	0.17177138973241923	1.4529047162947553E-4
62-63	1.7988344106506494E-4	0.0015497650307144057	0.0	0.17718173015214542	1.4529047162947553E-4
64-65	1.7988344106506494E-4	0.0016812183145696455	0.0	0.18234300119193536	1.4529047162947553E-4
66-67	1.7988344106506494E-4	0.0016846776115132045	0.0	0.187310551602886	1.591276594037113E-4
68-69	1.8680203495218282E-4	0.0017573228473279421	0.0	0.19277970007065268	1.7296484717794705E-4
70-71	1.8680203495218282E-4	0.001774619332045737	0.0	0.19794097111044262	1.7988344106506494E-4
72-73	1.8680203495218282E-4	0.0017780786289892958	0.0	0.20200564501912438	2.1447641050065437E-4
74-75	1.8680203495218282E-4	0.0018472645678604747	0.0	0.20527813992773114	2.179357074442133E-4
76-77	1.8680203495218282E-4	0.0019199098036752123	0.0	0.20822892022058692	2.2485430133133117E-4
78-79	2.006392227264186E-4	0.001944124882280125	0.0	0.21091333464878864	2.283135982748901E-4
80-81	2.006392227264186E-4	0.001951043476167243	0.0	0.21205144334321954	2.4561008299268483E-4
82-83	2.006392227264186E-4	0.0019648806639414786	0.0	0.21259801226030187	2.6636586465403843E-4
84-85	2.006392227264186E-4	0.001982177148659273	0.0	0.21292664546993995	2.698251615975974E-4
86-87	2.006392227264186E-4	0.0020271480089255394	0.0	0.2130304243782467	2.732844585411564E-4
88-89	2.006392227264186E-4	0.0021586012927807792	0.0	0.2130580987537952	2.767437554847153E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16230	0.0	54.05827	1
GTATCAA	30030	0.0	40.526836	1
ATCAACG	36215	0.0	32.777466	3
TCAACGC	36535	0.0	32.5515	4
TATCAAC	38620	0.0	30.973522	2
CAACGCA	38080	0.0	29.728014	5
CTTATAC	12905	0.0	29.22415	1
AACGCAG	39635	0.0	28.734375	6
ACGCAGA	45295	0.0	25.057772	7
CGCAGAG	45775	0.0	24.817068	8
TATACAC	18940	0.0	23.292635	3
GTGGTAT	7990	0.0	22.760826	1
TGGTATC	7980	0.0	21.223671	2
GCAGAGT	54095	0.0	20.787355	9
TTATACA	19955	0.0	20.337152	2
GAGTACT	33225	0.0	19.429298	12-13
GTACATG	35460	0.0	19.170143	1
ACATCTC	19085	0.0	18.984901	8
AGAGTAC	48600	0.0	18.952248	10-11
TACATGG	34760	0.0	18.837315	2
>>END_MODULE