FastQCFastQC Report
Fri 27 May 2016
SRR2139964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17151275
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195150.052.6801571
GTATCAA355800.040.6542781
ATCAACG429050.032.8798523
TCAACGC432950.032.6767274
CTTATAC158800.032.224021
TATCAAC451350.031.669242
CAACGCA448600.030.1079355
AACGCAG468300.028.9994136
ACGCAGA538750.025.2431377
CGCAGAG544700.024.9786518
TATACAC242300.024.7812213
TTATACA248300.022.003842
GCAGAGT641300.020.9765859
GTGGTAT92300.020.9153441
ACATCTC242150.020.1148728
GTACATG461300.019.412651
GAGTACT371700.019.3429312-13
TGGTATC92750.019.170672
TACATGG452300.019.0943762
AGAGTAC579450.018.76312310-11
Produced by FastQC (version 0.11.5)