##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17151275
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.771095559951082	33.0	31.0	34.0	30.0	34.0
2	31.48104114708673	33.0	31.0	34.0	30.0	34.0
3	31.794251972520993	33.0	31.0	34.0	30.0	34.0
4	33.15020428510417	37.0	35.0	37.0	28.0	37.0
5	34.52255934325582	37.0	35.0	37.0	30.0	37.0
6	35.15556534426741	37.0	35.0	37.0	32.0	37.0
7	35.25807270888024	37.0	35.0	37.0	32.0	37.0
8	35.39612221248858	37.0	35.0	37.0	32.0	37.0
9	37.13052020913897	39.0	37.0	39.0	33.0	39.0
10-11	37.0198946142488	39.0	37.0	39.0	33.0	39.0
12-13	36.91995711689073	39.0	37.0	39.0	32.5	39.0
14-15	38.24724476751729	40.0	38.0	41.0	33.0	41.0
16-17	38.24178750559361	40.0	38.0	41.0	32.5	41.0
18-19	38.18559299527294	40.0	38.0	41.0	32.5	41.0
20-21	38.24107971117404	40.0	38.0	41.0	32.5	41.0
22-23	38.27723838849194	40.0	38.0	41.0	32.5	41.0
24-25	38.17246744709187	40.0	38.0	41.0	32.0	41.0
26-27	38.08367187528688	40.0	38.0	41.0	32.0	41.0
28-29	38.082128628774186	40.0	38.0	41.0	32.0	41.0
30-31	38.03066786651814	40.0	37.5	41.0	32.0	41.0
32-33	37.940983292539364	40.0	37.0	41.0	32.0	41.0
34-35	37.840495938304706	40.0	37.0	41.0	31.0	41.0
36-37	37.79834644384802	40.0	37.0	41.0	31.0	41.0
38-39	37.72925861456629	40.0	37.0	41.0	31.0	41.0
40-41	37.6480283052053	40.0	37.0	41.0	31.0	41.0
42-43	37.62441600740932	40.0	37.0	41.0	31.0	41.0
44-45	37.56613047225636	40.0	37.0	41.0	31.0	41.0
46-47	37.44048785095353	40.0	36.0	41.0	30.0	41.0
48-49	37.617160735708936	40.0	37.0	41.0	31.0	41.0
50-51	37.577466440112374	40.0	36.5	41.0	31.0	41.0
52-53	37.518142352230285	40.0	36.0	41.0	31.0	41.0
54-55	37.38755081516243	39.5	36.0	41.0	30.5	41.0
56-57	37.2616509907793	39.0	35.5	41.0	30.0	41.0
58-59	37.15388771075705	39.0	35.0	41.0	30.5	41.0
60-61	36.945404557634745	39.0	35.0	41.0	30.5	41.0
62-63	36.76642700612011	39.0	35.0	41.0	30.0	41.0
64-65	36.5128693452175	38.0	35.0	40.0	30.0	41.0
66-67	36.29534750132818	37.5	35.0	40.0	30.0	41.0
68-69	36.043861835792846	37.0	34.0	40.0	30.0	41.0
70-71	35.79579044917075	37.0	34.0	39.0	30.0	41.0
72-73	35.493593445052184	36.0	34.0	39.0	30.0	41.0
74-75	35.31258251291745	36.0	34.0	39.0	30.0	40.5
76-77	35.11805100323944	35.5	34.0	38.5	30.0	40.0
78-79	34.8541026663292	35.0	34.0	37.0	30.0	39.0
80-81	34.594169272725594	35.0	34.0	37.0	30.0	39.0
82-83	34.341284945885015	35.0	34.0	36.0	30.0	39.0
84-85	34.12445114524114	35.0	34.0	36.0	30.0	37.0
86-87	33.93575942219985	35.0	34.0	36.0	29.5	37.0
88-89	33.7924298766405	35.0	34.0	35.0	29.0	37.0
90-91	33.65704304443744	35.0	34.0	35.0	29.0	36.0
92-93	33.611061225269694	35.0	34.0	35.0	29.0	36.0
94-95	33.58521584688734	35.0	34.0	35.0	29.5	36.0
96-97	33.59077554331938	35.0	34.0	35.0	30.0	36.0
98-99	33.62278033197521	35.0	34.0	35.0	30.0	35.5
100-101	33.22982203475118	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	17.0
17	171.0
18	1079.0
19	4198.0
20	11832.0
21	24879.0
22	42533.0
23	64597.0
24	93905.0
25	136851.0
26	195027.0
27	261199.0
28	288194.0
29	258948.0
30	312087.0
31	384648.0
32	482852.0
33	633334.0
34	863776.0
35	1216318.0
36	1858272.0
37	3204900.0
38	4320016.0
39	2373195.0
40	118442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01522918850056	20.717981607781347	14.693216685056942	24.573572518661148
2	14.112190158402921	21.119796845309526	41.86274354114231	22.905269455145245
3	17.42362263308223	26.40714790289679	30.96914001902601	25.200089444994973
4	11.971972111585357	18.626170668348806	39.02720214028842	30.374655079777412
5	13.000718199889524	37.776620360517924	35.76724073293004	13.455420706662514
6	29.165020038153745	38.64994452566158	18.953540715655436	13.231494720529238
7	25.720357093682765	33.07400588764511	23.375743762929414	17.82989325574271
8	23.868250277269457	37.27288596835228	21.422518536035934	17.436345218342336
9	25.123880284779474	17.094039170121615	21.397262564633827	36.38481798046509
10-11	23.694257153903703	27.041385380118122	29.266700392019228	19.997657073958948
12-13	24.625956575364754	24.58883855438141	29.225822964520283	21.559381905733556
14-15	21.80141231565135	25.98879732777728	26.788837441436534	25.42095291513483
16-17	21.437691751621813	29.191587216948218	27.425022767282385	21.945698264147588
18-19	21.48716394927934	28.20782707082772	29.141999405731617	21.16300957416133
20-21	22.280786617495785	27.352662295403174	28.999898532571045	21.366652554529995
22-23	21.91858649073436	27.47834993757187	28.87288140289313	21.730182168800635
24-25	21.775159278214986	27.603454209591483	28.960983988988403	21.66040252320513
26-27	21.763572034300093	27.74587877766778	28.899594790656646	21.590954397375477
28-29	21.501748242352843	27.604450587619255	29.07027130468836	21.82352986533954
30-31	21.951216302536093	27.37520654360918	29.11084984914563	21.562727304709092
32-33	21.66529183646882	27.74390437805627	28.907929558478006	21.682874226996898
34-35	21.71596568918653	27.753354074449515	29.0610325699247	21.469647666439258
36-37	21.922571072594366	27.567216850734365	28.832416022151573	21.677796054519693
38-39	21.68386330339552	27.58404163745852	28.97889792238012	21.753197136765845
40-41	21.834442378846244	27.48359805998061	28.941740123849264	21.740219437323884
42-43	21.90430925526747	27.501746179277937	28.82297170720393	21.770972858250666
44-45	21.845832957056942	27.709200626406943	28.607621284469015	21.8373451320671
46-47	22.09328144974872	27.557297990703184	28.195682494409642	22.15373806513846
48-49	21.9469073866425	27.64809693415208	28.290934119936356	22.114061559269064
50-51	21.84429325872546	27.86031532008877	28.22542948615364	22.069961935032126
52-53	21.96951149844829	27.848657289836588	28.2440815157782	21.937749695936926
54-55	21.97880016562816	27.805713664529442	28.108921593003178	22.10656457683922
56-57	22.08702081054807	27.992884680174445	27.912097978848323	22.00799653042916
58-59	21.868587101477416	28.150070658555304	28.150112644167287	21.831229595799993
60-61	22.0923974778498	28.261524052194957	27.829118991943975	21.816959478011267
62-63	21.92289216068564	28.42207065897009	27.807112860683027	21.84792431966124
64-65	21.958048646338586	28.29112341374164	27.822660958790173	21.928166981129593
66-67	22.001868577774054	28.26523056382299	27.74971671150111	21.983184146901852
68-69	22.004832241142445	28.092339973150732	27.851974849288013	22.050852936418806
70-71	22.0277501108583	27.793567932334696	27.964768527284868	22.21391342952214
72-73	22.096321636148303	27.80644423270352	27.92865761811819	22.168576513029986
74-75	21.950110763316953	27.904496420320818	27.918084007219985	22.227308809142244
76-77	22.05755934594931	27.893747153492715	27.87243673699318	22.17625676356479
78-79	22.0411271435669	27.93023596903248	27.795569678165315	22.233067209235305
80-81	22.01977803933924	27.898509910878527	27.838485490163734	22.2432265596185
82-83	22.064448804873642	27.876627157019946	27.907912984628506	22.1510110534779
84-85	22.164476411403683	27.85890890807468	27.838027493886873	22.138587186634762
86-87	21.988616291487887	27.884147722616948	27.904685963357423	22.22255002253774
88-89	22.070159626013684	27.902942905349704	27.840415073304765	22.186482395331847
90-91	22.11519656819388	27.9022584651304	27.87046543777715	22.11207952889857
92-93	22.10750986227711	27.887381713125375	27.86321320181514	22.141895222782374
94-95	21.98427644893104	27.95335493262783	27.896668108438515	22.16570051000262
96-97	21.999112630521633	27.973632449785708	27.907321968975175	22.119932950717487
98-99	22.26487251177226	28.526602967144694	27.95660985659247	21.25191466449058
100-101	22.900270865530747	31.12574838482732	25.118270377583702	20.85571037205823
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3723.0
1	3159.0
2	3805.0
3	5203.0
4	6745.5
5	10788.0
6	14200.0
7	16811.0
8	18655.0
9	18382.0
10	16442.0
11	14306.0
12	15601.0
13	21209.0
14	35322.5
15	55294.5
16	71852.0
17	81505.0
18	83748.0
19	80100.5
20	74023.5
21	68890.0
22	69444.5
23	78863.5
24	96466.0
25	122714.0
26	157634.5
27	196102.5
28	236169.0
29	278059.5
30	316909.0
31	354672.5
32	395027.5
33	435954.0
34	476452.5
35	514523.0
36	553535.5
37	586759.5
38	609003.0
39	629686.5
40	655159.5
41	678790.5
42	692627.5
43	707456.0
44	731052.5
45	753553.5
46	754358.0
47	746344.0
48	730963.0
49	703268.5
50	680249.0
51	653318.5
52	618075.0
53	580381.0
54	541469.0
55	499292.0
56	455551.0
57	412084.5
58	368030.5
59	319719.0
60	266546.5
61	214823.5
62	169448.0
63	131997.0
64	100383.5
65	75009.5
66	56145.0
67	42824.0
68	32596.0
69	23837.0
70	17844.5
71	13281.0
72	9583.0
73	6755.5
74	4602.0
75	3221.0
76	2266.0
77	1548.5
78	1075.0
79	750.5
80	539.0
81	399.0
82	306.0
83	222.5
84	164.5
85	119.5
86	83.5
87	62.0
88	44.0
89	33.5
90	27.5
91	22.0
92	17.5
93	8.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6037933039963501
3	0.006238603252527873
4	8.032638972904346
5	3.5565869009738344E-4
6	0.07416358259079865
7	0.02240066700580569
8	5.189118593224119E-4
9	2.273883428491468E-4
10-11	0.25922854131835676
12-13	0.2703938919992828
14-15	5.830470329465302E-5
16-17	1.078637010951081E-4
18-19	0.41658710504029584
20-21	1.8660641767461345E-4
22-23	1.137441786677226E-4
24-25	0.15336369058754346
26-27	0.14228172294859476
28-29	8.76223346527115E-5
30-31	1.0235235541136025E-4
32-33	9.67191742363098E-5
34-35	2.586533887341459E-4
36-37	2.4198860570090333E-4
38-39	0.00145426498375957
40-41	0.0018041920521815354
42-43	0.001178942829934369
44-45	9.127588433216696E-4
46-47	0.0014696758115611067
48-49	0.0025713938858681878
50-51	2.587686565186537E-4
52-53	0.0015747068263635216
54-55	4.1517794209668536E-4
56-57	0.007791149848662671
58-59	1.4856425245665525E-4
60-61	0.0027899690724586296
62-63	0.001308558012249619
64-65	0.0015052166914278143
66-67	0.0047542191590655
68-69	0.0021531280112278813
70-71	0.0025655636869288154
72-73	0.0036363200246112277
74-75	7.327449743470564E-4
76-77	3.453806618305303E-4
78-79	7.81409117582411E-4
80-81	4.2988900193107594E-4
82-83	0.0015703491538638056
84-85	2.4237297008908735E-4
86-87	3.395362583602315E-4
88-89	1.1837316023766123E-4
90-91	2.2430257272343815E-4
92-93	0.0
94-95	0.0
96-97	8.478045153341797E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1825.0
20-21	4400.0
22-23	5646.0
24-25	7151.0
26-27	9883.0
28-29	16075.0
30-31	36155.0
32-33	45188.0
34-35	61374.0
36-37	89659.0
38-39	108292.0
40-41	110480.0
42-43	128907.0
44-45	156041.0
46-47	155210.0
48-49	144018.0
50-51	131411.0
52-53	129356.0
54-55	139602.0
56-57	150568.0
58-59	157494.0
60-61	155474.0
62-63	153117.0
64-65	161331.0
66-67	171635.0
68-69	178161.0
70-71	164367.0
72-73	148268.0
74-75	149651.0
76-77	154684.0
78-79	160762.0
80-81	161897.0
82-83	155896.0
84-85	154623.0
86-87	157948.0
88-89	163729.0
90-91	170465.0
92-93	171822.0
94-95	187152.0
96-97	286755.0
98-99	1383795.0
100-101	1.0771008E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.67720026280547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.38157853558891	75.68581825463737
2	7.369312271895402	12.480254620928738
3	1.8324542180594854	4.655012783851369
4	0.6515768838258819	2.206948251133557
5	0.3106707898376042	1.3153366343441382
6	0.1631896158825412	0.8291063870937745
7	0.08228989971295196	0.4877654822319868
8	0.05745402030498829	0.3892036466615028
9	0.03624640578899584	0.2762319745621541
>10	0.1126650907231419	1.4328382861875604
>50	0.0020806833973424426	0.12012091655164703
>100	4.247728317045829E-4	0.06262714077495246
>500	3.411237051247046E-5	0.019898502766247078
>1k	2.2699780511677205E-5	0.038837118274979196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.830470329465302E-6
2	0.0	1.1660940658930604E-5	0.0	0.0	5.830470329465302E-6
3	0.0	1.1660940658930604E-5	0.0	0.0	5.830470329465302E-6
4	5.830470329465302E-6	5.247423296518772E-5	0.0	0.0	1.1660940658930604E-5
5	5.830470329465302E-6	6.413517362411832E-5	0.0	0.0	1.1660940658930604E-5
6	5.830470329465302E-6	6.413517362411832E-5	0.0	0.0	1.7491410988395906E-5
7	5.830470329465302E-6	6.996564395358362E-5	0.0	0.0	2.3321881317861208E-5
8	5.830470329465302E-6	8.162658461251424E-5	0.0	0.0	2.915235164732651E-5
9	5.830470329465302E-6	1.0494846593037544E-4	0.0	3.498282197679181E-5	4.081329230625712E-5
10-11	5.830470329465302E-6	1.0494846593037544E-4	0.0	3.498282197679181E-5	4.081329230625712E-5
12-13	5.830470329465302E-6	1.0494846593037544E-4	0.0	3.498282197679181E-5	4.081329230625712E-5
14-15	5.830470329465302E-6	1.428465230718999E-4	0.0	3.498282197679181E-5	4.955899780045507E-5
16-17	5.830470329465302E-6	1.5159222856609786E-4	0.0	3.498282197679181E-5	5.830470329465302E-5
18-19	5.830470329465302E-6	1.661684043897611E-4	0.0	4.3728527470989764E-5	7.579611428304893E-5
20-21	5.830470329465302E-6	1.8074458021342436E-4	0.0	4.6643762635722416E-5	9.03722901067122E-5
22-23	5.830470329465302E-6	2.098969318607509E-4	0.0	5.247423296518772E-5	1.25355112083504E-4
24-25	5.830470329465302E-6	2.1572740219021617E-4	0.0	8.745705494197953E-5	1.6033793406029583E-4
26-27	5.830470329465302E-6	2.2155787251968147E-4	0.0	1.8365981537815703E-4	1.719988747192264E-4
28-29	5.830470329465302E-6	2.244731076844141E-4	0.0	7.550459076657566E-4	1.7491410988395905E-4
30-31	5.830470329465302E-6	2.50710224167008E-4	0.0	0.003055166452639818	1.7782934504869172E-4
32-33	8.745705494197953E-6	2.50710224167008E-4	0.0	0.008518317151348806	1.9823599120182028E-4
34-35	1.1660940658930604E-5	2.944387516379978E-4	0.0	0.015873455471969285	2.157274021902162E-4
36-37	1.4576175823663255E-5	3.4399774943845283E-4	0.0	0.026420776297972015	2.1864263735494883E-4
38-39	1.7491410988395906E-5	3.7898057141524466E-4	0.0	0.04048095549747759	2.390492835080774E-4
40-41	1.7491410988395906E-5	4.489462153688283E-4	0.0	0.05372195361569329	2.390492835080774E-4
42-43	1.7491410988395906E-5	4.6352239119249155E-4	0.0	0.0656598416152735	2.390492835080774E-4
44-45	1.7491410988395906E-5	5.130813889929467E-4	0.0	0.07852768963240342	2.4779498900227533E-4
46-47	1.7491410988395906E-5	5.568099164639363E-4	0.0	0.09078625350010422	2.6237116482593855E-4
48-49	1.7491410988395906E-5	5.655556219581343E-4	0.0	0.10143269232170786	2.8569304614379983E-4
50-51	1.7491410988395906E-5	6.35521265911718E-4	0.0	0.11069147920489877	2.8860828130853244E-4
52-53	1.7491410988395906E-5	6.442669714059159E-4	0.0	0.1186238340881363	3.0609969229692833E-4
54-55	1.7491410988395906E-5	6.500974417353812E-4	0.0	0.12639876627247829	3.206758681205916E-4
56-57	1.7491410988395906E-5	6.530126769001139E-4	0.0	0.13384136164804075	3.265063384500569E-4
58-59	1.7491410988395906E-5	6.588431472295791E-4	0.0	0.14004206684342707	3.4399774943845283E-4
60-61	2.0406646153128555E-5	6.792497933827077E-4	0.0	0.1463185681530965	3.4399774943845283E-4
62-63	2.3321881317861208E-5	6.821650285474403E-4	0.0	0.15202076813531357	3.498282197679181E-4
64-65	2.3321881317861208E-5	7.812830241483504E-4	0.0	0.15752473212632878	3.527434549326508E-4
66-67	2.3321881317861208E-5	7.929439648072811E-4	0.0	0.16289751053493107	3.5565869009738344E-4
68-69	2.3321881317861208E-5	7.987744351367464E-4	0.0	0.16853557534352404	3.585739252621161E-4
70-71	2.3321881317861208E-5	8.01689670301479E-4	0.0	0.1739812346312446	3.7023486592104666E-4
72-73	2.3321881317861208E-5	8.046049054662117E-4	0.0	0.17803924198055246	4.197938637215018E-4
74-75	2.3321881317861208E-5	8.250115516193403E-4	0.0	0.18139176241999502	4.197938637215018E-4
76-77	2.3321881317861208E-5	8.512486681019341E-4	0.0	0.1845781144550478	4.2270909888623444E-4
78-79	2.3321881317861208E-5	8.570791384313994E-4	0.0	0.18743212968132106	4.2562433405096705E-4
80-81	2.3321881317861208E-5	8.599943735961321E-4	0.0	0.1886390370395204	4.4020050987463033E-4
82-83	2.3321881317861208E-5	8.629096087608648E-4	0.0	0.18912296607686602	4.751833318514221E-4
84-85	2.3321881317861208E-5	8.6874007909033E-4	0.0	0.18945238765048078	4.780985670161548E-4
86-87	2.3321881317861208E-5	8.949771955729239E-4	0.0	0.18957482752739957	4.92674742839818E-4
88-89	2.3321881317861208E-5	9.328752527144483E-4	0.0	0.18960106464388216	4.955899780045507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19515	0.0	52.680157	1
GTATCAA	35580	0.0	40.654278	1
ATCAACG	42905	0.0	32.879852	3
TCAACGC	43295	0.0	32.676727	4
CTTATAC	15880	0.0	32.22402	1
TATCAAC	45135	0.0	31.66924	2
CAACGCA	44860	0.0	30.107935	5
AACGCAG	46830	0.0	28.999413	6
ACGCAGA	53875	0.0	25.243137	7
CGCAGAG	54470	0.0	24.978651	8
TATACAC	24230	0.0	24.781221	3
TTATACA	24830	0.0	22.00384	2
GCAGAGT	64130	0.0	20.976585	9
GTGGTAT	9230	0.0	20.915344	1
ACATCTC	24215	0.0	20.114872	8
GTACATG	46130	0.0	19.41265	1
GAGTACT	37170	0.0	19.34293	12-13
TGGTATC	9275	0.0	19.17067	2
TACATGG	45230	0.0	19.094376	2
AGAGTAC	57945	0.0	18.763123	10-11
>>END_MODULE