FastQCFastQC Report
Fri 27 May 2016
SRR2139963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9371342
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117500.054.5344351
GTATCAA209400.041.3505821
CTTATAC101750.039.5408971
ATCAACG257150.033.0239523
TCAACGC265300.032.064
TATACAC137650.031.9127253
TATCAAC273050.031.4585672
CAACGCA271750.029.7632165
AACGCAG284800.028.609796
TTATACA159100.026.3583182
ACGCAGA318750.025.449757
CGCAGAG325750.024.9352668
ACATCTC166150.022.7698848
ACACATC169750.022.1598116
GCAGAGT367800.021.899519
TACACAT195900.021.227225
ATACACA229600.020.689254
CACATCT187950.019.8630947
CATCTCC191250.019.7147069
TACATGG253050.019.6465782
Produced by FastQC (version 0.11.5)